PROTEIN NAME | SITE | SCORE | PROTEIN NOTES | PMID REFERENCE | REFERENCE | ARTICLE NOTES | GENE/CONSTRUCT (Target RNA) | SPLICING ASSAY | BINDING ASSAY |
9G8 | ACACGACGAU | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAAUGAU | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAGACUA | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAGAGAA | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAGAGAC | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAGAGAU | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAGAUCA | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAUAGAA | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAUAGAC | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAUUGAC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACGAUUGAU | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | ACUAGAGAC | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACAACGAU | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACAACGUU | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACCACGCU | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACGACGAA | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACGACGAU | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACGACGUU | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACAAG | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACACC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACAUA | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACAUC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACGAC | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACGAG | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACGAU | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACGCU | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUACGUU | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AGACUUCGAU | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AUACGACGAG | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | AUCCGACGAG | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CAACGACGAG | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CAGAGAGAC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CCGAGAUCA | 2 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CCGAGAUCU | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CGACGAGGAC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CGACGAUGAC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CUACGACGAG | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | CUGAGAGAC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | UAGAGAGAC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | UCGAGAGAU | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | UCGAUUGAC | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | UGACGACGAA | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | UGACGACGAU | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
9G8 | GGACGACGA | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
9G8 | AAAGGACAAA | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
9G8 | UCUUCA | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
9G8 | UCUCCG | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
9G8 | UGGACAA | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
9G8 | GGAAGAAGAUAAAGAC | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 12826680 | Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003) The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20. J Biol Chem. 278(35):32943-32953. | Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10. | In vivo splicing in SVK14 and HEK293-EBNA cells. | UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts. | |
9G8 | GACGACGAG | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 10523623 | Bourgeois CF, Popielarz M, Hildwein G, Stevenin J. (1999) Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation. Mol Cell Biol. 19(11):7347-7356. | Construct and variants of Adenovirus E1A unit. | in vitro splicing with Hela nuclear extracts, in vivo splicing in Hela cells, in vitro complementation assays. | UV crosslink, immunoprecipitation. | |
9G8 | AGAGGAAGGCGA | 7 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
9G8 | AGGAGCAGGGGACGAAG | 10 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
9G8 | GAAGAAGAA | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
9G8 | CUAGCUCACGCAGCAUAUCUCACGCAUCAUG | 2 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
9G8 | ACAACAAGAAGACGCGCAUCAU | 2 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11336712 | Huang Y, Steitz JA. (2001) Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA. Mol Cell. 7(4):899-905. | Sequences wt and mutated deriving from mouse Histone 2A | UV cross-linking and immunoprecipitation with HeLa NE | ||
9G8 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
9G8 | UCAACA | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 8769651 | Lynch KW, Maniatis T. (1996) Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer. Genes Dev. 10(16):2089-2101. | Sequences deriving from D. melanogaster dsx [40940]. | UV-cross link, immunoprecipitation with HeLa extracts. | ||
9G8 | GACGACGAGGAGCAGCAG | 8 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11454855 | Tian H, Kole R. (2001) Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein. J Biol Chem. 276(36):33833-33839. | Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2). | In vitro splicing with HeLa NE | Filter binding assay, UV cross-linking and immunoprecipitation with HeLa NE | |
9G8 | GACGACUCAGCAG | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 11454855 | Tian H, Kole R. (2001) Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein. J Biol Chem. 276(36):33833-33839. | Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2). | In vitro splicing with HeLa NE | Filter binding assay, UV cross-linking and immunoprecipitation with HeLa NE | |
9G8 | CUCUUCAC | 5 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
9G8 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | 6 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
9G8 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | 4 | Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20 The shuttling protein 9G8 binds TAP and can function as export factors (18364396). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
CUG-BP1 | UGUGU | -2 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUG | -3 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUGU | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUGUG | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUGUGU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUGUGUG | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUGUGUGU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUUGUGUGU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUGUGUGUGUGUGU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | UGUUUGU | -2 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 16938098 | Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006) CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding. Biochem J. 400(2): 291-301. | Sequences of 35nt random for SELEX. | SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA. | ||
CUG-BP1 | CUGCUGCUGCUGCUGCUGCUGCUG | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 8789448 | Timchenko LT, Timchenko NA, Caskey CT, Roberts R. (1996) Novel proteins with binding specificity for DNA CTG repeats and RNA CUG repeats: implications for myotonic dystrophy. Hum Mol Genet. 5(1):115-121. | CUG-BP1 has no affinity to (CGG)8 repeats. | synthesized oligos | EMSA with HeLa whole cell extract. | |
CUG-BP1 | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUG | -10 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCG | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | UCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUG | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGA | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | UCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCG | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGA | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUG | -2 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCG | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | UAUGUAUGUAUGUAUGUAUGUAUGUAUG | -6 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CAUGCAUGCAUGCAUGCAUGCAUGCAUG | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG | -2 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CCUGCCUGCCUGCCUGCCUGCCUGCCUG | -2 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG | -2 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
CUG-BP1 | CUGUCUG | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 12649496 | Gromak N, Matlin AJ, Cooper TA, Smith CW. (2003) Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein. RNA. 9(4):443-456. | Construct of rat Actn1 [81634] EX_NM - INT | In vitro splicing with HeLa nuclear extracts. | UV cross-link with recombinant protein and with HeLa nuclear extract containing increasing protein concentrations. Mutation analysis. | |
CUG-BP1 | CUGCUGCUGCUGCUGCUGCUGCUGCUGCUG | -10 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 8912635 | Bhagavati S, Ghatpande A, Leung B. (1996) Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy. Biochem Biophys Res Commun. 228(1):55-62. | Synthesized sequences | EMSA, UV-crosslink, protein purification | ||
CUG-BP1 | CUCUCUCUCUCUCUCUCUCUCUCUCUCUCU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 8912635 | Bhagavati S, Ghatpande A, Leung B. (1996) Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy. Biochem Biophys Res Commun. 228(1):55-62. | Synthesized sequences | EMSA, UV-crosslink, protein purification | ||
CUG-BP1 | UUUUUUUGUUGUGUUUUUUCCUU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 8912635 | Bhagavati S, Ghatpande A, Leung B. (1996) Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy. Biochem Biophys Res Commun. 228(1):55-62. | Synthesized sequences | EMSA, UV-crosslink, protein purification | ||
CUG-BP1 | UGUGUGUGUGUGUGUGUGUGUGUUUUU | -4 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
CUG-BP1 | UGUGUGUGUGUGUGUGUGUGUGUGUUUUU | -6 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
CUG-BP1 | UGUGUGUGUGUGUGUGUGUGUGUGUGUUUUU | -8 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
CUG-BP1 | UGUGUGUGUGUGUGUGUGUGUGUGUGUGUG | -10 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | UGUGUGUGUGUGUAUGUGUGUGUGUGUGUG | -9 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | UAUGUAUGUAUGUAUGUAUGUAUGUAUGUA | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | GUUGGUUGGUUGGUUGGUUGGUUGGUUGGU | -3 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | UGGUGGUGGUGGUGGUGGUGGUGGUGGUGG | -3 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | UUGUUGUUGUUGUUGUUGUUGUUGUUGUUG | -3 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | CUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUG | -1 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18039683 | Mori D, Sasagawa N, Kino Y, Ishiura S. (2008) Quantitative analysis of CUG-BP1 binding to RNA repeats. J Biochem. 143(3):377-383. | Synthesized sequences | Surface plasmon resonance (SPR) with recombinant protein. | ||
CUG-BP1 | UUUCUUGUUUGUUUGUUUGGGU | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18243120 | Vlasova IA, Tahoe NM, Fan D, Larsson O, Rattenbacher B, Sternjohn JR, Vasdewani J, Karypis G, Reilly CS, Bitterman PB, Bohjanen PR. (2008) Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1. Mol Cell. 29(2):263-270. | Sequences deriving from wt and mutated JUN [3725], JUNB [3726], TNFRSF1B [7133]. | Mutagenesis, EMSA with cytoplasmic extracts and supershift, UV cross-linking, siRNA-knockdown in primary human T cells and HeLa. | ||
CUG-BP1 | GUGUUUGUGUUUGUGUGUGUUUGUU | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18243120 | Vlasova IA, Tahoe NM, Fan D, Larsson O, Rattenbacher B, Sternjohn JR, Vasdewani J, Karypis G, Reilly CS, Bitterman PB, Bohjanen PR. (2008) Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1. Mol Cell. 29(2):263-270. | Sequences deriving from wt and mutated JUN [3725], JUNB [3726], TNFRSF1B [7133]. | Mutagenesis, EMSA with cytoplasmic extracts and supershift, UV cross-linking, siRNA-knockdown in primary human T cells and HeLa. | ||
CUG-BP1 | GUUUGUUUGUUUGUUUGUUUGUUU | -5 | Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2. Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906). | 18243120 | Vlasova IA, Tahoe NM, Fan D, Larsson O, Rattenbacher B, Sternjohn JR, Vasdewani J, Karypis G, Reilly CS, Bitterman PB, Bohjanen PR. (2008) Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1. Mol Cell. 29(2):263-270. | Sequences deriving from wt and mutated JUN [3725], JUNB [3726], TNFRSF1B [7133]. | Mutagenesis, EMSA with cytoplasmic extracts and supershift, UV cross-linking, siRNA-knockdown in primary human T cells and HeLa. | ||
DAZAP1 | UUUUUUU | -7 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 10857750 | Tsui S, Dai T, Roettger S, Schempp W, Salido EC, Yen PH. (2000) Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1. Genomics. 65(3):266-273. | DAZAP1 has no affinity to poly (C). | Homopolymers | In vitro RNA-binding assay of homopolymers and SDS-PAGE with recombinant protein. | |
DAZAP1 | GGGGGGG | -7 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 10857750 | Tsui S, Dai T, Roettger S, Schempp W, Salido EC, Yen PH. (2000) Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1. Genomics. 65(3):266-273. | DAZAP1 has no affinity to poly (C). | Homopolymers | In vitro RNA-binding assay of homopolymers and SDS-PAGE with recombinant protein. | |
DAZAP1 | AAAAAAA | -3 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 10857750 | Tsui S, Dai T, Roettger S, Schempp W, Salido EC, Yen PH. (2000) Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1. Genomics. 65(3):266-273. | DAZAP1 has no affinity to poly (C). | Homopolymers | In vitro RNA-binding assay of homopolymers and SDS-PAGE with recombinant protein. | |
DAZAP1 | AGAAC | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 18503770 | Skoko N, Baralle M, Buratti E, Baralle FE. (2008) The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors. FEBS Lett. 582(15):2231-2236. | Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37. | In vivo splicing in HeLa, siRNA. | UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts. | |
DAZAP1 | UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
DAZAP1 | UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
DAZAP1 | UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
DAZAP1 | UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
DAZAP1 | UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
DAZAP1 | UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
DAZAP1 | UGCAGAUGCUUAGUUUGUGU | -8 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
DAZAP1 | UGCAGAUGGUUAGUUUGUGU | -10 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
DAZAP1 | AGAUAU | 8 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
DAZAP1 | AAUUUA | 8 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
DAZAP1 | AGUAGG | 5 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
DAZAP1 | GUAACG | 8 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
DAZAP1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -6 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
DAZAP1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -4 | Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
ESRP1 | GUGUGGUGAUGGGCCUGCAGAGGUGAGCUGGCCGGUGUCUCUC | 5 | Gene Name and Synonymous: ESRP1, epithelial splicing regulatory protein 1, RBM35A, RMB35A, FLJ20171 It promotes exon inclusion or exclusion depending on epithelial or mesenchymal cells. | 19285943 | Warzecha CC, Sato TK, Nabet B, Hogenesch JB, Carstens RP. (2009) ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing. Mol Cell. 33(5):591-601. | Sequences deriving from wt and mutated rat Fgfr2 [25022]. | In vivo splicing in PNT2. | Pull-down and immunoblotting with KATO III NE | |
ESRP1 | AGGGAU | 5 | Gene Name and Synonymous: ESRP1, epithelial splicing regulatory protein 1, RBM35A, RMB35A, FLJ20171 It promotes exon inclusion or exclusion depending on epithelial or mesenchymal cells. | 20526337 | Dominguez C, Fisette JF, Chabot B, Allain FH. (2010) Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs. Nat Struct Mol Biol. 17(7):853-861. | Synthesized sequences. Constructs of wt and mutant BCL2L1 [598] EX2 - INT2 - EX3. | In vitro splicing assays in HeLa nuclear extracts. | NMR spectroscopy. In vitro splicing assays in HeLa nuclear extracts with increasing amount of splicing factor. | |
ESRP2 | GUGUGGUGAUGGGCCUGCAGAGGUGAGCUGGCCGGUGUCUCUC | 5 | Gene Name and Synonymous: ESRP2, epithelial splicing regulatory protein 2, RBM35B, FLJ21918, FLJ22248 It promotes exon inclusion or exclusion depending on epithelial or mesenchymal cells. | 19285943 | Warzecha CC, Sato TK, Nabet B, Hogenesch JB, Carstens RP. (2009) ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing. Mol Cell. 33(5):591-601. | Sequences deriving from wt and mutated rat Fgfr2 [25022]. | In vivo splicing in PNT2. | Pull-down and immunoblotting with KATO III NE | |
ETR-3 | AUGUG | 7 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | AUGUU | 8 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | CGUGU | 6 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | GUCUGU | 4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | GUGUG | 8 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | GUUGU | 8 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UAUGU | 9 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UGUGU | 7 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UGUUC | 6 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UGUUG | 7 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UGUUU | 6 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UUGUU | 6 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | CAUCG | 2 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | CUGCUGCUGCUGCUGCUGCUGCUG | 5 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 8948631 | Timchenko LT, Miller JW, Timchenko NA, DeVore DR, Datar KV, Lin L, Roberts R, Caskey CT, Swanson MS. (1996) Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy. Nucleic Acids Res. 24(22): 4407-4414. | Synthesized sequences. | Purification of HeLa extract by DEAE chromatography and detection by bandshift/supershift analysis. | ||
ETR-3 | GUGUGU | 7 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | AUGUGU | 7 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UGUGUG | 7 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UGUUGU | 6 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | GUAUGU | 4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | GUUGUU | 4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UAUGUG | 4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UAUGUU | 4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | UUGUGU | 4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 15657417 | Faustino NA, Cooper TA. (2005) Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment. Mol Cell Biol. 25 (3): 879-887. | Sequences of 20nt random for SELEX. | SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA. | ||
ETR-3 | CUGCUGCUGCUGCUGCUGCUGCUGCUGCUG | -10 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 8912635 | Bhagavati S, Ghatpande A, Leung B. (1996) Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy. Biochem Biophys Res Commun. 228(1):55-62. | Synthesized sequences | EMSA, UV-crosslink, protein purification | ||
ETR-3 | CUCUCUCUCUCUCUCUCUCUCUCUCUCUCU | -8 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 8912635 | Bhagavati S, Ghatpande A, Leung B. (1996) Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy. Biochem Biophys Res Commun. 228(1):55-62. | Synthesized sequences | EMSA, UV-crosslink, protein purification | ||
ETR-3 | UUUUUUUGUUGUGUUUUUUCCUU | -8 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 8912635 | Bhagavati S, Ghatpande A, Leung B. (1996) Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy. Biochem Biophys Res Commun. 228(1):55-62. | Synthesized sequences | EMSA, UV-crosslink, protein purification | ||
ETR-3 | UGUGUGUGUGUGUGUGUGUGUGUUUUU | -4 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
ETR-3 | UGUGUGUGUGUGUGUGUGUGUGUGUUUUU | -6 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
ETR-3 | UGUGUGUGUGUGUGUGUGUGUGUGUGUUUUU | -8 | Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3. Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542). | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
FMRP | GAAGAGGACAAGGAGGAAGAGGACGUGGAGGAGGC | 5 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11532944 | Schaeffer C, Bardoni B, Mandel JL, Ehresmann B, Ehresmann C, Moine H. (2001) The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif. EMBO J. 20(17): 4803-4813. | This long motif form a correct G-quartet structure. | Synthesized sequences. | EMSA, competition assay, ladder selection with recombinant protein. | |
FMRP | AAGAGAGGGAGAGCUUCCUGCGCAGAGGAGACGGACGGCGGCGUGGAGGGGGAGGAAGAGGACAAGGAGGAAGAGGACGUGGAGGAGGCUUCAAAGGAAAC | 10 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11532944 | Schaeffer C, Bardoni B, Mandel JL, Ehresmann B, Ehresmann C, Moine H. (2001) The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif. EMBO J. 20(17): 4803-4813. | This long motif form a correct G-quartet structure. | Synthesized sequences. | EMSA, competition assay, ladder selection with recombinant protein. | |
FMRP | GGAGGAAGAGGACAAGGAGGAAGAGGACGUGGAGGAGGCUUCAAA | 5 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 18653529 | Didiot MC, Tian Z, Schaeffer C, Subramanian M, Mandel JL, Moine H. (2008) The G-quartet containing FMRP binding site in FMR1 mRNA is a potent exonic splicing enhancer. Nucleic Acids Res. 36(15): 4902-4912. | This short motif is still able to form a G-quartet structure. | Construct of beta-globin [3043] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4, with putative and mutant ESEs inserted in EX2. | In vivo splicing in HeLa cells. | Site directed mutagenesis, EMSA, competition assay. |
FMRP | GCUGCGGUGUGGAAGGAGUGGCUGGGUUGCGCAGC | 10 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11719189 | Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001) Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function. Cell. 107(4): 489-499. | Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. | Synthesized sequences. | Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein. | |
FMRP | AAGGGUAGGAUGGGAUGGCUGGCGAGG | 2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11719189 | Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001) Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function. Cell. 107(4): 489-499. | Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. | Synthesized sequences. | Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein. | |
FMRP | AAGGUAGGGUGGUUGGG | 2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11719189 | Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001) Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function. Cell. 107(4): 489-499. | Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. | Synthesized sequences. | Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein. | |
FMRP | GUGGGUGGUUGGGUGG | 2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11719189 | Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001) Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function. Cell. 107(4): 489-499. | Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. | Synthesized sequences. | Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein. | |
FMRP | GAGGAGUUGGAAGGAUGGG | 2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 11719189 | Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001) Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function. Cell. 107(4): 489-499. | Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. | Synthesized sequences. | Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein. | |
FMRP | AAGCGGCUGG | 10 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GAGCGAAGGGAG | 7 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GAGCGACUGG | 6 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GAGCGACUGGUG | 2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GAGCGGCUGG | 7 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGACUAAGGAGU | 8 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCAUAGGCAC | 8 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCGAAGGAAG | 10 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCGAAGGAGU | 9 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCGAAGGGAG | 10 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUAAGGAAU | 6 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUAAGGAGU | 2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUAAGGAUU | 7 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUAUGGAGU | 6 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUGAAGGAAC | 8 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUGAAGGAAG | 4 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGCUGAGGAUG | 6 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GUGCGACUGGGC | 4 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GUGCGGCUGGGC | 8 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | UAGCAGCUGG | 7 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | UAGCGACUGG | 10 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | UAGCGGCUGG | 7 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | UAGUGGCUGG | 8 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 15805463 | Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005) Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes. Genes Dev. 19(8): 903-918. | Sequences of 52nt random for SELEX. | SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein. | ||
FMRP | GGGGUUGGGGAUUUAGCUCAGUGGUAGAGCGCUUGCCUAGCAAGCGCAAGGCCCUGGGUUCGGUCCUCAGCUCUGG | 5 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 16006558 | Zalfa F, Adinolfi S, Napoli I, Kuhn-Holsken E, Urlaub H, Achsel T, Pastore A, Bagni C. (2005) Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif. J Biol Chem. 280(39): 33403-33410. | Synthesized sequences, Rodent brain-specific BC1 RNA. | EMSA, competition assay with recombinant protein. | ||
FMRP | GGGGGGG | 7 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 7688265 | Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. (1993) The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein. Cell. 74(2):291-298. | Homopolymers | Homopolymer binding assay with recombinant protein | ||
FMRP | UUUUUUU | 4 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 7688265 | Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. (1993) The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein. Cell. 74(2):291-298. | Homopolymers | Homopolymer binding assay with recombinant protein | ||
FMRP | GGCUGCGGUGUGGAAGGUGAGGCUGGGUUGCGCAGCU | 5 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 16819831 | Zanotti KJ, Lackey PE, Evans GL, Mihailescu MR. (2006) Thermodynamics of the fragile X mental retardation protein RGG box interactions with G quartet forming RNA. Biochemistry. 45(27):8319-8330. | Synthesized sequences | EMSA with recombinant protein. Fluorescence and Circular Dichroism Spectroscopy. | ||
FMRP | AAAAAAA | -2 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 8917439 | Dejgaard K, Leffers H. (1996) Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains. Eur J Biochem. 241(2):425-431. | Synthesized oligos | Homopolymer binding assay with recombinant protein. | ||
FMRP | GGCUGGUGAUUGGAAGGGAGGGAGGUGGCCAGCC | 5 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 17693432 | Menon L, Mihailescu MR. (2007) Interactions of the G quartet forming semaphorin 3F RNA with the RGG box domain of the fragile X protein family. Nucleic Acids Res. 35(16):5379-5392. | Synthesized sequences | EMSA supershift with recombinant protein. Fluorescence, UV and CD spectroscopy. | ||
FMRP | GGAUUGGAAGGGAGGGAGGUG | 5 | Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1. FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463). | 19396385 | Bole M, Menon L, Mihailescu MR. (2008) Fragile X mental retardation protein recognition of G quadruplex structure per se is sufficient for high affinity binding to RNA. Mol Biosyst. 4(12):1212-1219. | Synthesized sequences | Fluorescence, UV and CD spectroscopy with recombinant protein. | ||
Fox-1 | AGCAUG | 1 | Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1. Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 16537540 | Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006) Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16. J Biol Chem. 281(18): 12468-12474. | Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay. | |
Fox-1 | UGCAUG | 10 | Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1. Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 16537540 | Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006) Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16. J Biol Chem. 281(18): 12468-12474. | Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay. | |
Fox-1 | UGCAUG | -5 | Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1. Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 17101796 | Zhou HL, Baraniak AP, Lou H. (2007) Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Mol Cell Biol. 27(3): 830-841. | Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4. | In vivo splicing in HeLa cell. | siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation. | |
Fox-1 | UGACUG | -5 | Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1. Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 17101796 | Zhou HL, Baraniak AP, Lou H. (2007) Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Mol Cell Biol. 27(3): 830-841. | Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4. | In vivo splicing in HeLa cell. | siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation. | |
Fox-2 | UGCAUG | 10 | Gene Name and Synonymous: RBFOX2, RNA binding protein, fox-1 homolog (C. elegans) 2, RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3. Fox-2 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 16537540 | Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006) Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16. J Biol Chem. 281(18): 12468-12474. | Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay. | |
Fox-2 | UGCAUG | -5 | Gene Name and Synonymous: RBFOX2, RNA binding protein, fox-1 homolog (C. elegans) 2, RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3. Fox-2 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 17101796 | Zhou HL, Baraniak AP, Lou H. (2007) Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Mol Cell Biol. 27(3): 830-841. | Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4. | In vivo splicing in HeLa cell. | siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation. | |
Fox-2 | UGACUG | -5 | Gene Name and Synonymous: RBFOX2, RNA binding protein, fox-1 homolog (C. elegans) 2, RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3. Fox-2 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796). | 17101796 | Zhou HL, Baraniak AP, Lou H. (2007) Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing. Mol Cell Biol. 27(3): 830-841. | Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4. | In vivo splicing in HeLa cell. | siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation. | |
hnRNP A0 | AUUUA | -3 | Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. | 7585247 | Myer VE, Steitz JA. (1995) Isolation and characterization of a novel, low abundance hnRNP protein: A0. RNA. 1(2): 171-182. | Sequence of 30nt. | RNA affinity chromatography with HeLa nuclear extract confirmed by UV crosslink | ||
hnRNP A0 | AGAUAU | -8 | Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A0 | AAUUUA | -10 | Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A0 | AGUAGG | -9 | Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A0 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -6 | Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP A0 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -4 | Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP A1 | UAGGAA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | UAGAGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | UAGGAU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | UAGGUA | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | UAGGCU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | CAGGGA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | UAGGGA | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | UAGGGU | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 7510636 | Burd CG, Dreyfuss G. (1994) RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing. EMBO J. 13(5): 1197-1204. | Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear extracts. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract | |
hnRNP A1 | AUUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 8473331 | Hamilton BJ, Nagy E, Malter JS, Arrick BA, Rigby WF. (1993) Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences. J Biol Chem. 268(12): 8881-7. | Partial sequence of colony stimulating factor 2 CSF2 [1437] 3'UTR. | UV crosslink and immunoprecipitation with cytoplasmic lysates of lymphocytes. | ||
hnRNP A1 | UAGACA | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12833158 | Kashima T, Manley JL. (2003) A negative element in SMN2 exon 7 inhibits splicing in spinal muscular atrophy. Nat Genet. 34(4): 460-463. | Constructs and mutants of SMN1 [6606] and SMN2 [6607] EX7. | In vivo splicing in HeLa. | UV crosslink, SDS-PAGE, immunoprecipitation, siRNA and Western blot. | |
hnRNP A1 | AUAGAA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12419255 | Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, Branlant C. (2002) A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding. J Mol Biol. 323(4): 629-652. | Constructs of HIV-1 A7 3' splice site | In vitro splicing with HeLa cell nuclear or cytoplasmic S100 extracts. | Competition assay, UV crosslink, immunoprecipitation with HeLa nuclear extracts, EMSA and supershift assays. | |
hnRNP A1 | CUAGACUAGA | -6 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 10406810 | Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J. 18(14):4060-4067. | Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2 | In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay. | RNA affinity chromatography, immunoblotting. | |
hnRNP A1 | AGAAC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18503770 | Skoko N, Baralle M, Buratti E, Baralle FE. (2008) The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors. FEBS Lett. 582(15):2231-2236. | Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37. | In vivo splicing in HeLa, siRNA. | UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts. | |
hnRNP A1 | GGAGGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP A1 | UAGGGCAGGC | -6 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9858549 | Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999) hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol. 19(1):251-260. | Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing. | In vivo splicing in HeLa, SVK14 and 293 cells. | UV crosslink, immunoprecipitation in HeLa cell nuclear extracts. | |
hnRNP A1 | UAGGGC | -6 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9858549 | Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999) hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol. 19(1):251-260. | Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing. | In vivo splicing in HeLa, SVK14 and 293 cells. | UV crosslink, immunoprecipitation in HeLa cell nuclear extracts. | |
hnRNP A1 | UAGAGU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9121425 | Chabot B, Blanchette M, Lapierre I, La Branche H. (1997) An intron element modulating 5' splice site selection in the hnRNP A1 pre-mRNA interacts with hnRNP A1. Mol Cell Biol. 17(4):1776-1786. | Construct of murine hnRNP A1 [15382] EX7 - 7b - partial_EX8. | In vitro splicing assay in HeLa nuclear extracts. In vivo splicing assay using tranfected HeLa cells. | RNase protection, immunoprecipitation in HeLa nuclear extract. | |
hnRNP A1 | UAGGCA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11598017 | Tange TO, Damgaard CK, Guth S, Valcarcel J, Kjems J. (2001) The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element. EMBO J. 20(20):5748-5758. | Construct of HIV-1 Tat [155871] INT2. | In vitro splicing with HeLa nuclear extracts, immunodepletion. | UV crosslink, immunoblot, EMSA. | |
hnRNP A1 | UAGUGA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11598017 | Tange TO, Damgaard CK, Guth S, Valcarcel J, Kjems J. (2001) The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element. EMBO J. 20(20):5748-5758. | Construct of HIV-1 Tat [155871] INT2. | In vitro splicing with HeLa nuclear extracts, immunodepletion. | UV crosslink, immunoblot, EMSA. | |
hnRNP A1 | UUUUUUU | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 8449401 | Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B. (1993) Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev. 7(3):393-406. | PSF has no affinity to poly(rA), poly(rC) and poly(rG). | Construct of tropomyosin 1 alpha TPM1 [7168] EX2 - INT2 - EX3 for splicing. Construct of branchpoint and polypyrimidine tract element upstream of TPM1 EX3 for UV-crosslink. | In vitro splicing in HeLa nuclear extracts. | RNA affinity chromatography confirmed by UV crosslink and Western blot using HeLa nuclear extracts. |
hnRNP A1 | UUAGAUUAGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9275159 | Li HP, Zhang X, Duncan R, Comai L, Lai MM. (1997) Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA. Proc Natl Acad Sci U S A. 94(18): 9544-9549. | Sequence of (-)-strand leader RNA of mouse hepatitis virus (MHV). Intergenic sequences of mouse hepatitis virus (MHV). | UV crosslink with cytoplasmic extracts from HeLa cells. Protein identified by peptide sequencing and immunoprecipitation. | ||
hnRNP A1 | GUUUAGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9275159 | Li HP, Zhang X, Duncan R, Comai L, Lai MM. (1997) Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA. Proc Natl Acad Sci U S A. 94(18): 9544-9549. | Sequence of (-)-strand leader RNA of mouse hepatitis virus (MHV). Intergenic sequences of mouse hepatitis virus (MHV). | UV crosslink with cytoplasmic extracts from HeLa cells. Protein identified by peptide sequencing and immunoprecipitation. | ||
hnRNP A1 | CAAGCACCGAACCCGCAACUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A1 | GCCAAGGAGCCAGAGAGCAUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A1 | GCCCUUGGGUUUGCAUGCCACUGCAUGAGAGACGUUUAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16537540 | Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006) Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16. J Biol Chem. 281(18): 12468-12474. | Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay. | |
hnRNP A1 | GCCCUUGGGUUUGACUGCCACUGACUGAGAGACGUUUAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16537540 | Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006) Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16. J Biol Chem. 281(18): 12468-12474. | Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay. | |
hnRNP A1 | CGUAGGUC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP A1 | UGAAGUAGAUUAGCAUCUACUGCCCUAAGUGCUCCUUCUGGCA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17558416 | Guil S, Caceres JF. (2007) The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a. Nat Struct Mol Biol. 14(7): 591-596. | hnRNP A1 facilitates processing of miR-18a. | HeLa endogenous RNAs. | In vivo UV cross-linking and immunoprecipitation (CLIP) in HeLa cells. | |
hnRNP A1 | UAGGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP A1 | UAGUUAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP A1 | CUUAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP A1 | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
hnRNP A1 | UCGGGC | -3 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9858549 | Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999) hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol. 19(1):251-260. | Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing. | In vivo splicing in HeLa, SVK14 and 293 cells. | UV crosslink, immunoprecipitation in HeLa cell nuclear extracts. | |
hnRNP A1 | UACGGC | -3 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9858549 | Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999) hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol. 19(1):251-260. | Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing. | In vivo splicing in HeLa, SVK14 and 293 cells. | UV crosslink, immunoprecipitation in HeLa cell nuclear extracts. | |
hnRNP A1 | UAGAUCCUAGACUAGAGCCCUG | -6 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9858549 | Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999) hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol. 19(1):251-260. | Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing. | In vivo splicing in HeLa, SVK14 and 293 cells. | UV crosslink, immunoprecipitation in HeLa cell nuclear extracts. | |
hnRNP A1 | UAGGGACUUA | -6 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 10406810 | Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J. 18(14):4060-4067. | Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2 | In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay. | RNA affinity chromatography, immunoblotting. | |
hnRNP A1 | GAGUGGUGCG | -6 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 10406810 | Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J. 18(14):4060-4067. | Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2 | In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay. | RNA affinity chromatography, immunoblotting. | |
hnRNP A1 | GUAAGUACGC | -3 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 10406810 | Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J. 18(14):4060-4067. | Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2 | In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay. | RNA affinity chromatography, immunoblotting. | |
hnRNP A1 | UAGUGAA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11779509 | Zhu J, Mayeda A, Krainer AR. (2001) Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell. 8(6): 1351-1361. | Sequence of HIV-1 Tat [155871] EX3. | In Vitro Splicing with HeLa S100 and HeLa nuclear extracts. | UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot. | |
hnRNP A1 | AAGGUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP A1 | CUGAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP A1 | AUAGCA | -9 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12426391 | Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002) Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch. EMBO J. 21(22):6195-6204. | Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17 | In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells. | RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting. | |
hnRNP A1 | UUUAUA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12426391 | Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002) Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch. EMBO J. 21(22):6195-6204. | Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17 | In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells. | RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting. | |
hnRNP A1 | AUUUAA | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12426391 | Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002) Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch. EMBO J. 21(22):6195-6204. | Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17 | In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells. | RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting. | |
hnRNP A1 | UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | CUGAUUUGUAUUUAUUAGACUC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | AACAGAAAAAGAAAUAUUU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | CUGAUUUGUACCUAUUAGAUUC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | UACUGAAGAACAAGUAUUU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A1 | CCAUGGUUUGGGGGCAGUAGUUGG | -1 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP A1 | AAGAAUGGGAAUAUUUUAUACUGACAGAAAUCAGUAAUAUUUAUAUAUUUAUAUUUUUAAAAUAUUUAUUUAUUUAUUUAUUUAAGUUCAUAUUCCAUAUUUAUUCAAGAUGUUUUACCGUAAUAAUUAUUAUUAAAAAUAUGCUUCUAAA | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9353343 | Hamilton BJ, Burns CM, Nichols RC, Rigby WF. (1997) Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation. J Biol Chem. 272(45):28732-28741. | Synthesized sequences and sequence deriving from CSF2 [1437] and HBB [3043] | EMSA, UV cross-linking, immunoblotting, competition assay with human T lymphocytes extracts and recombinant protein | ||
hnRNP A1 | GGAUCCAUUUAUUUAUUUAUUUAAGCUUGG | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9353343 | Hamilton BJ, Burns CM, Nichols RC, Rigby WF. (1997) Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation. J Biol Chem. 272(45):28732-28741. | Synthesized sequences and sequence deriving from CSF2 [1437] and HBB [3043] | EMSA, UV cross-linking, immunoblotting, competition assay with human T lymphocytes extracts and recombinant protein | ||
hnRNP A1 | UUGGUAUCAAGGUUACAAGACAGGUUUAAGGAGACCAAUAGAAACUGGGCAUGUGGAGACAGAGAAGACUCUUGGGUUUCUGAUAGGCACUGACUCUCUCUGCCUAUUGGUCUAUUUUCCCACCCUUAG | -3 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9353343 | Hamilton BJ, Burns CM, Nichols RC, Rigby WF. (1997) Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation. J Biol Chem. 272(45):28732-28741. | Synthesized sequences and sequence deriving from CSF2 [1437] and HBB [3043] | EMSA, UV cross-linking, immunoblotting, competition assay with human T lymphocytes extracts and recombinant protein | ||
hnRNP A1 | UAGGUUAGGAAUAGGGAAUUAAGG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9740129 | Mayeda A, Munroe SH, Xu RM, Krainer AR. (1998) Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1. RNA. 4(9):1111-1123. | Synthesized sequences | Filter binding assay with recombinant protein | ||
hnRNP A1 | UUAGAUCGAUGGGAAAAAAUUCGGUUAAGG | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9925777 | Najera I, Krieg M, Karn J. (1999) Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1. J Mol Biol. 285(5):1951-1964. | p17gag [155030] and synthesized sequences. | Mutation analysis, EMSA supershift and UV cross-linking in HeLa NE. | ||
hnRNP A1 | UAUGAUAGGGACUUAGGGUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 9925777 | Najera I, Krieg M, Karn J. (1999) Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1. J Mol Biol. 285(5):1951-1964. | p17gag [155030] and synthesized sequences. | Mutation analysis, EMSA supershift and UV cross-linking in HeLa NE. | ||
hnRNP A1 | CUAGUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | GAAGUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | CUUCUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | CUAGUACUAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | CUAGUAGAUUCUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | CUAGUAGAAACUAUUCUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | GAUCUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | AGCUAGAUUAGACUUC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A1 | AGCUAAGACUGGGGGCAGGAUGGCGGAAAGGAAGGGGCGUGGUGGCUAGAGGGAAGAGAAGAAACAGAAGCGGCUCAGUUCACCUUGAUAAGGUGCUUCCGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 15155834 | Christian K, Lang M, Maurel P, Raffalli-Mathieu F. (2004) Interaction of heterogeneous nuclear ribonucleoprotein A1 with cytochrome P450 2A6 mRNA: implications for post-transcriptional regulation of the CYP2A6 gene. Mol Pharmacol. 65(6):1405-1414. | Sequences deriving from CYP2A6 [1548] | UV cross-linking in primary hepatocyte NE and HepG2 NE | ||
hnRNP A1 | AGGGUUGAGGGGAGCAGGGU | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP A1 | AUUUAUUU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 15514164 | Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004) RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res. 95(11):1058-1066. | Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] | EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF. | ||
hnRNP A1 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP A1 | UAGGGAUAGGGUUAGGGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16157593 | Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005) TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing. J Biol Chem. 280(45):37572-37584. | Synthesized sequences | EMSA with recombinant protein | ||
hnRNP A1 | GGUUUCAGACAAAAUCA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16385450 | Cartegni L, Hastings ML, Calarco JA, de Stanchina E, Krainer AR. (2006) Determinants of exon 7 splicing in the spinal muscular atrophy genes, SMN1 and SMN2. Am J Hum Genet. 78(1):63-77. | Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vitro splicing in HeLa | RNA affinity chromatography with HeLa NE, western blot. | |
hnRNP A1 | GGUUUUAGACAAAAUCA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16385450 | Cartegni L, Hastings ML, Calarco JA, de Stanchina E, Krainer AR. (2006) Determinants of exon 7 splicing in the spinal muscular atrophy genes, SMN1 and SMN2. Am J Hum Genet. 78(1):63-77. | Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vitro splicing in HeLa | RNA affinity chromatography with HeLa NE, western blot. | |
hnRNP A1 | GAGGAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16990281 | Hallay H, Locker N, Ayadi L, Ropers D, Guittet E, Branlant C. (2006) Biochemical and NMR study on the competition between proteins SC35, SRp40, and heterogeneous nuclear ribonucleoprotein A1 at the HIV-1 Tat exon 2 splicing site. J Biol Chem. 281(48):37159-37174. | Sequences deriving from HIV-1 Tat [155871] | Competition assays with recombinant protein | ||
hnRNP A1 | UAUAAUGUUCUCUUUUUAGAAAAGGU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17287399 | Lewis SM, Veyrier A, Hosszu Ungureanu N, Bonnal S, Vagner S, Holcik M. (2007) Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translation. Mol Biol Cell. 18(4):1302-1311. | Sequences deriving from XIAP [331] | UV cross-linking, competition assay with recombinant protein | ||
hnRNP A1 | GGACAAGUCCUAUUUUCAAGAGAAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17287399 | Lewis SM, Veyrier A, Hosszu Ungureanu N, Bonnal S, Vagner S, Holcik M. (2007) Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translation. Mol Biol Cell. 18(4):1302-1311. | Sequences deriving from XIAP [331] | UV cross-linking, competition assay with recombinant protein | ||
hnRNP A1 | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP A1 | CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP A1 | GAGAAUUCGCUGGUCUCGAACUCCUGACCUCAAGUGAUCCCACCGAAUUCGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17353911 | Donev R, Newall A, Thome J, Sheer D. (2007) A role for SC35 and hnRNPA1 in the determination of amyloid precursor protein isoforms. Mol Psychiatry. 12(7):681-690. | Synthesized sequences | EMSA with recombinant protein | ||
hnRNP A1 | GAGAAUUCGCUCCUGACCUCAAGUGAUCCCACCGAAUUCGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17353911 | Donev R, Newall A, Thome J, Sheer D. (2007) A role for SC35 and hnRNPA1 in the determination of amyloid precursor protein isoforms. Mol Psychiatry. 12(7):681-690. | Synthesized sequences | EMSA with recombinant protein | ||
hnRNP A1 | CAGAAGGGGAGGGGUUCCA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP A1 | AACAAGGGGUGGGGGAAAA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP A1 | AUGUCUCUUUGGCUGCCUAGUGAGGCCACUGUCUACUUGCCUCCUGUCCCAGUAUCUAAGGUUGUAAGCACGGAUGAAUAUGUUGCACGCACAAACAUAUAUUAUCAUGCAGGAACAUCCAGACUACUU | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17869320 | Zhao X, Fay J, Lambkin H, Schwartz S. (2007) Identification of a 17-nucleotide splicing enhancer in HPV-16 L1 that counteracts the effect of multiple hnRNP A1-binding splicing silencers. Virology. 369(2):351-363. | Sequences deriving from HPV-16 L1 mRNA and synthesized sequences. | UV cross-linking with HeLa NE and recombinant protein | ||
hnRNP A1 | UAGUGUAGUGUAGUGUAGUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17869320 | Zhao X, Fay J, Lambkin H, Schwartz S. (2007) Identification of a 17-nucleotide splicing enhancer in HPV-16 L1 that counteracts the effect of multiple hnRNP A1-binding splicing silencers. Virology. 369(2):351-363. | Sequences deriving from HPV-16 L1 mRNA and synthesized sequences. | UV cross-linking with HeLa NE and recombinant protein | ||
hnRNP A1 | AGACCUUUAGGGUUAGGGACAUCC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17925491 | Eiring AM, Neviani P, Santhanam R, Oaks JJ, Chang JS, Notari M, Willis W, Gambacorti-Passerini C, Volinia S, Marcucci G, Caligiuri MA, Leone GW, Perrotti D. (2008) Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis. Blood. 111(2):816-828. | Sequences deriving from E2F3 [1871] and synthesized sequences. | EMSA, UV cross-linking, competition assays, western blot with K562 cytoplasmic extracts. | ||
hnRNP A1 | AGACCUCUAGGGAGAAAGACAUCC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17925491 | Eiring AM, Neviani P, Santhanam R, Oaks JJ, Chang JS, Notari M, Willis W, Gambacorti-Passerini C, Volinia S, Marcucci G, Caligiuri MA, Leone GW, Perrotti D. (2008) Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis. Blood. 111(2):816-828. | Sequences deriving from E2F3 [1871] and synthesized sequences. | EMSA, UV cross-linking, competition assays, western blot with K562 cytoplasmic extracts. | ||
hnRNP A1 | GCUAAACGCAAAAAACGUAAGCUGUAAGUAUUGUAUGUAUGUUGAAUUAGUGUUGUUUGUUGUGUAUAUGUUUGUAUGU | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 17950949 | Cheunim T, Zhang J, Milligan SG, McPhillips MG, Graham SV. (2008) The alternative splicing factor hnRNP A1 is up-regulated during virus-infected epithelial cell differentiation and binds the human papillomavirus type 16 late regulatory element. Virus Res. 131(2):189-198. | Sequences deriving from HPV-16 LRE. | EMSA supershift, UV cross-linking with HeLa NE and W12E NE. EMSA with recombinant protein. | ||
hnRNP A1 | CAGCAUUAUGAAAG | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A1 | CCGCAUUAUGAAAG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A1 | CAGCAUUAUGAACG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A1 | CCGCAUUAUGAACG | -1 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A1 | UGCAGAUGCUUAGUUUGUGU | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
hnRNP A1 | UGCAGAUGGUUAGUUUGUGU | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
hnRNP A1 | CCCCCCC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 3733753 | Kumar A, Williams KR, Szer W. (1986) Purification and domain structure of core hnRNP proteins A1 and A2 and their relationship to single-stranded DNA-binding proteins. J Biol Chem. 261(24):11266-11273. | Synthesized oligos | Filter binding assay with purified protein | ||
hnRNP A1 | AAAAAAA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 3733753 | Kumar A, Williams KR, Szer W. (1986) Purification and domain structure of core hnRNP proteins A1 and A2 and their relationship to single-stranded DNA-binding proteins. J Biol Chem. 261(24):11266-11273. | Synthesized oligos | Filter binding assay with purified protein | ||
hnRNP A1 | GUAUCCUUCCCUGGCCGUGAUAGUAGAGCAGCAGGGCCAGGGGGGUGACACACC | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP A1 | AGGUAGGGCCCUAAGGGCA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 20010808 | David CJ, Chen M, Assanah M, Canoll P, Manley JL. (2010) HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. 463(7279):364-368. | Sequences deriving from PKM2 [5315]. Construct of PKM2 [5315] EX9 - INT9 - AdML. Construct of PKM2 [5315] EX8 - INT8 - EX9 - INT9 - EX10 - INT10 - EX11. | In vitro splicing with HeLa NE. In vivo splicing in HeLa. | Protein affinity purification, mass spectrometry, UV cross-linking and immunoblotting with HeLa NE. siRNA. | |
hnRNP A1 | AGGUAGGGCCCUAAGGGCA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 20010808 | David CJ, Chen M, Assanah M, Canoll P, Manley JL. (2010) HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. 463(7279):364-368. | Sequences deriving from PKM2 [5315]. Construct of PKM2 [5315] EX9 - INT9 - AdML. Construct of PKM2 [5315] EX8 - INT8 - EX9 - INT9 - EX10 - INT10 - EX11. | In vitro splicing with HeLa NE. In vivo splicing in HeLa. | Protein affinity purification, mass spectrometry, UV cross-linking and immunoblotting with HeLa NE. siRNA. | |
hnRNP A1 | AUAUAUUUAAUCUUAAUCUGUUUAUUUACAAGGGAAGAUUUAUGUUUGGUGAACUAUAUUA | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18846111 | Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. (2009) hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ. 16(2):244-252. | Sequences deriving fromwt and mutated BIRC2 [329] | RNA-af?nity chromatography, western blot with HEK293 protein extracts. UV cross-linking with recombinant protein. | ||
hnRNP A1 | AUAUAGCAAUCUUAAUCUGUUUAUUUACAAGGGAAGAUUUAUGUUUGGUGAACUAUAUUA | -3 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18846111 | Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. (2009) hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ. 16(2):244-252. | Sequences deriving fromwt and mutated BIRC2 [329] | RNA-af?nity chromatography, western blot with HEK293 protein extracts. UV cross-linking with recombinant protein. | ||
hnRNP A1 | AUAUAUUUAAUCUUAAUCUGUUUAGCACAAGGGAAGAUUUAUGUUUGGUGAACUAUAUUA | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18846111 | Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. (2009) hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA. Cell Death Differ. 16(2):244-252. | Sequences deriving fromwt and mutated BIRC2 [329] | RNA-af?nity chromatography, western blot with HEK293 protein extracts. UV cross-linking with recombinant protein. | ||
hnRNP A1 | CAAAAAGAAGGAAGGUGCUCACAU | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19953646 | Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010) A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy. Hum Mutat. 31(1):E1110-1125. | Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein | |
hnRNP A1 | CAAAAAGAACGAAGGUGCUCACAU | -4 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 19953646 | Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010) A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy. Hum Mutat. 31(1):E1110-1125. | Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein | |
hnRNP A1 | UAUGAUAGGCUCAUAGGGUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UAUGAUAGGCACUUAGGCUG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UUAGAUCGAUGGGAAAAAAUUCGUUAAGG | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UUAGAUCGAUCCCAAAAAAUUCGUUAAGG | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UAAAUGUGGGGACCUAGAGGAGGAGCUGAAAAUUGUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UAAACUACGCGACCUAGAGGAGGAGCUGAAAAUUGUUA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UAUGACCCGGACUCCCGGUG | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | UGCAAUGUACUUGCAAACAAUGGCCUGAGUGUGCAAAGAAAAUGUCUGCUAACUGC | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP A1 | AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP A1 | AUUUUCCUUACAGGGUUUCAGACAAAAUCAAAAAG | -4 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP A1 | GAAUGGCCGGAGGAGAUUCAGCCUGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 20120036 | Homolova K, Zavadakova P, Doktor TK, Schroeder LD, Kozich V, Andresen BS. (2010) The deep intronic c.903+469T>C mutation in the MTRR gene creates an SF2/ASF binding exonic splicing enhancer, which leads to pseudoexon activation and causes the cblE type of homocystinuria. Hum Mutat. 31(4):437-444. | Sequences deriving from wt or mutated MTRR [4552]. Constructs of wt or mutated HBB [3043]_EX1 - MTRR [4552]_INT6 - HBB [3043]_EX2 and Tat [155871]_EX1 - MTRR [4552]_INT6 - Tat [155871]_EX2. | In vivo splicing in HEK293, protein overexpression and siRNA knockdown | Pulldown and Western blotting with HeLa NE | |
hnRNP A1 | GAAUGGCUGGAGGAGAUUCAGCCUGA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 20120036 | Homolova K, Zavadakova P, Doktor TK, Schroeder LD, Kozich V, Andresen BS. (2010) The deep intronic c.903+469T>C mutation in the MTRR gene creates an SF2/ASF binding exonic splicing enhancer, which leads to pseudoexon activation and causes the cblE type of homocystinuria. Hum Mutat. 31(4):437-444. | Sequences deriving from wt or mutated MTRR [4552]. Constructs of wt or mutated HBB [3043]_EX1 - MTRR [4552]_INT6 - HBB [3043]_EX2 and Tat [155871]_EX1 - MTRR [4552]_INT6 - Tat [155871]_EX2. | In vivo splicing in HEK293, protein overexpression and siRNA knockdown | Pulldown and Western blotting with HeLa NE | |
hnRNP A1 | UUUAGUCAGCCUUAUAGCUAA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
hnRNP A1 | GAGGAAG | 5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 23430061 | Oh HK, Lee E, Jang HN, Lee J, Moon H, Sheng Z, Jun Y, Loh TJ, Cho S, Zhou J, Green MR, Zheng X, Shen H. (2013) hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene. Apoptosis. 18(7):825-835. | Constructs of wt or mutated FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. Synthesized sequences. | In vivo splicing in HeLa, HCT-116, MDA-MB-231. | In vivo splicing using wt and mutated sequences in MDA-MB-231, HeLa, HCT-116 | |
hnRNP A1 | CAAAGAGGAA | 5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 23430061 | Oh HK, Lee E, Jang HN, Lee J, Moon H, Sheng Z, Jun Y, Loh TJ, Cho S, Zhou J, Green MR, Zheng X, Shen H. (2013) hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene. Apoptosis. 18(7):825-835. | Constructs of wt or mutated FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. Synthesized sequences. | In vivo splicing in HeLa, HCT-116, MDA-MB-231. | In vivo splicing using wt and mutated sequences in MDA-MB-231, HeLa, HCT-116 | |
hnRNP A1 | AGAUAU | -8 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A1 | AAUUUA | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A1 | AGUAGG | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A1 | UUCGAUUAGUGAA | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCCAUUAGUGAA | -4 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGCUUAGUGAA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGACUAGUGAA | -10 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGAUCAGUGAA | -7 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGAUUCGUGAA | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGAUUACUGAA | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCCAUUACUGAA | -3 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGCUUCGUGAA | -1 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | UUCGACCAGUGAA | -9 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24628426 | Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014) Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element. Biochemistry. 53(13):2172-2184. | RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. | Sequences deriving from HIV-1 Tat [155871] | Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences. | |
hnRNP A1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -5 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 24371143 | Zamiri B, Reddy K, Macgregor RB Jr, Pearson CE. (2014) TMPyP4 porphyrin distorts RNA G-quadruplex structures of the disease-associated r(GGGGCC)n repeat of the C9orf72 gene and blocks interaction of RNA-binding proteins. J Biol Chem. 289(8):4653-4659. | Synthetic sequences. | EMSA with recombinant protein. | ||
hnRNP A1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP A1 | GCAGUGACGACGCGCUGCUCAAGAACUACGGUCUGCUCUCCUGCUUCCGGAAGGACCUGCAUAAGACGGAGACGUACCUGAGGGUCAUGAAGUGCCGCCGCUUCGGGGAGGCCAG | -2 | Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835. hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471). | 8276242 | Sun Q, Mayeda A, Hampson RK, Krainer AR, Rottman FM. (1993) General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes Dev. 7(12B):2598-2608. | Construct of cow GH1 [280804] EX4 - INT4 - EX5 | In vitro splicing in HeLa nuclear extract, using also competing RNAs or protein excess | UV crosslinking and immunoprecipitation in HeLa nuclear extract, UV crosslinking with purified HeLa protein or recombinant protein | |
hnRNP A2/B1 | GCCAAGGAGCC | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A2/B1 | AGAAC | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18503770 | Skoko N, Baralle M, Buratti E, Baralle FE. (2008) The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors. FEBS Lett. 582(15):2231-2236. | Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37. | In vivo splicing in HeLa, siRNA. | UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts. | |
hnRNP A2/B1 | CUAGACUAGA | -4 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 10406810 | Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999) hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J. 18(14):4060-4067. | Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2 | In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay. | RNA affinity chromatography, immunoblotting. | |
hnRNP A2/B1 | CAAGCACCGAACCCGCAACUG | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A2/B1 | GCGAAGGAGCC | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A2/B1 | GCCAAGAAGCC | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A2/B1 | GCCAAGGGGCC | -2 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A2/B1 | GCCAAGGAACC | -2 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 11024030 | Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000) Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2. J Biol Chem. 275(49): 38286-38295. | synthesized oligos | UV crosslink, EMSA, IAsys resonant mirror biosensor. | ||
hnRNP A2/B1 | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
hnRNP A2/B1 | UAGACA | -8 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12833158 | Kashima T, Manley JL. (2003) A negative element in SMN2 exon 7 inhibits splicing in spinal muscular atrophy. Nat Genet. 34(4): 460-463. | Constructs and mutants of SMN1 [6606] and SMN2 [6607] EX7. | In vivo splicing in HeLa. | UV crosslink, SDS-PAGE, immunoprecipitation, siRNA and Western blot. | |
hnRNP A2/B1 | AUAGCA | -9 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12426391 | Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002) Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch. EMBO J. 21(22):6195-6204. | Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17 | In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells. | RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting. | |
hnRNP A2/B1 | UUUAUA | -7 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12426391 | Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002) Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch. EMBO J. 21(22):6195-6204. | Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17 | In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells. | RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting. | |
hnRNP A2/B1 | AUUUAA | -2 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12426391 | Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002) Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch. EMBO J. 21(22):6195-6204. | Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17 | In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells. | RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting. | |
hnRNP A2/B1 | UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A2/B1 | UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A2/B1 | UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A2/B1 | UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A2/B1 | UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A2/B1 | UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP A2/B1 | CUAGUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | GAAGUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | CUUCUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | CUAGUACUAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | CUAGUAGAUUCUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | CUAGUAGAAACUAUUCUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | GAUCUAGAAACUAAACUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 12060656 | Hutchison S, LeBel C, Blanchette M, Chabot B. (2002) Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor. J Biol Chem. 277(33):29745-29752. | Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EX | In vitro splicing in HeLa NE | EMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay. | |
hnRNP A2/B1 | UAGGGAUAGGGUUAGGGA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 16157593 | Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005) TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing. J Biol Chem. 280(45):37572-37584. | Synthesized sequences | EMSA with recombinant protein | ||
hnRNP A2/B1 | GCCAAGGAGCCAGAGAGCAUG | -7 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 16548521 | Landsberg MJ, Moran-Jones K, Smith R. (2006) Molecular recognition of an RNA trafficking element by heterogeneous nuclear ribonucleoprotein A2. Biochemistry. 45(12):3943-3951. | Synthesized sequences | Pull-down assay, UV cross-linking, CD spectroscopy with recombinant protein | ||
hnRNP A2/B1 | CAGCAUUAUGAAAG | -10 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A2/B1 | CCGCAUUAUGAAAG | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A2/B1 | CAGCAUUAUGAACG | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A2/B1 | CCGCAUUAUGAACG | -1 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18371932 | Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008) Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice. Am J Hum Genet. 82(4):834-848. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vitro splicing in HeLa NE and in vivo splicing in HEK293. | RNA-affinity chromatography, western blot with HeLa NE. | |
hnRNP A2/B1 | UGCAGAUGCUUAGUUUGUGU | -8 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
hnRNP A2/B1 | UGCAGAUGGUUAGUUUGUGU | -10 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
hnRNP A2/B1 | GUAUCCUUCCCUGGCCGUGAUAGUAGAGCAGCAGGGCCAGGGGGGUGACACACC | -2 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP A2/B1 | CCAAGGAGCCAGAGAGCAUGG | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18480411 | Raju CS, Goritz C, Nord Y, Hermanson O, Lopez-Iglesias C, Visa N, Castelo-Branco G, Percipalle P. (2008) In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences. Mol Biol Cell. 19(7):3008-3019. | Sequences deriving from mouse Mbp [17196]. | Protein affinity purification and EMSA with HeLa NE, cytoplasmic or protein extracts | ||
hnRNP A2/B1 | AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP A2/B1 | UUUAGUCAGCCUUAUAGCUAA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
hnRNP A2/B1 | AGAUAU | -1 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A2/B1 | AAUUUA | -5 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A2/B1 | AGUAGG | -9 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP A2/B1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP A2/B1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244. hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP A3 | CCAAGGAGCCAGAGAGCAUGG | -5 | Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. | 18480411 | Raju CS, Goritz C, Nord Y, Hermanson O, Lopez-Iglesias C, Visa N, Castelo-Branco G, Percipalle P. (2008) In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences. Mol Biol Cell. 19(7):3008-3019. | Sequences deriving from mouse Mbp [17196]. | Protein affinity purification and EMSA with HeLa NE, cytoplasmic or protein extracts | ||
hnRNP A3 | AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG | -5 | Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP A3 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP A3 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP C | AUUUA | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 8473331 | Hamilton BJ, Nagy E, Malter JS, Arrick BA, Rigby WF. (1993) Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences. J Biol Chem. 268(12): 8881-7. | Partial sequence of colony stimulating factor 2 CSF2 [1437] 3'UTR. | UV crosslink and immunoprecipitation with cytoplasmic lysates of lymphocytes. | ||
hnRNP C | AGUAUUUUUGUGGA | -10 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 9649627 | Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998) Oligonucleotide binding specificities of the hnRNP C protein tetramer. Nucleic Acids Res. 26(14): 3410-3417. | hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. | synthesized oligos | Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions. | |
hnRNP C | GGGGGGGGGGGGGGGGGGGG | -10 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 9649627 | Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998) Oligonucleotide binding specificities of the hnRNP C protein tetramer. Nucleic Acids Res. 26(14): 3410-3417. | hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. | synthesized oligos | Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions. | |
hnRNP C | AGUAGGGGGGUGGA | -8 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 9649627 | Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998) Oligonucleotide binding specificities of the hnRNP C protein tetramer. Nucleic Acids Res. 26(14): 3410-3417. | hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. | synthesized oligos | Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions. | |
hnRNP C | GAUCACUUGUGUCAACACAG | -8 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 9649627 | Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998) Oligonucleotide binding specificities of the hnRNP C protein tetramer. Nucleic Acids Res. 26(14): 3410-3417. | hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. | synthesized oligos | Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions. | |
hnRNP C | AUCGCUUCUCGGCCUUUUGGCUAAGAUCA | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 8798770 | Temsamani J, Pederson T. (1996) The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle. J Biol Chem. 271(40): 24922-24926. | hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. | Sequences of U2 snRNA [6066] and homopolymers | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
hnRNP C | UUUUUUU | -7 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 8798770 | Temsamani J, Pederson T. (1996) The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle. J Biol Chem. 271(40): 24922-24926. | hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. | Sequences of U2 snRNA [6066] and homopolymers | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
hnRNP C | UGGAUUUUUUUCGGGUA | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 12509468 | Kim JH, Paek KY, Choi K, Kim TD, Hahm B, Kim KT, Jang SK. (2003) Heterogeneous nuclear ribonucleoprotein C modulates translation of c-myc mRNA in a cell cycle phase-dependent manner. Mol Cell Biol. 23(2):708-720. | Synthesized sequences. | UV cross-linking, Immunoprecipitation, deletion assay | ||
hnRNP C | GGGGGGG | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 12509468 | Kim JH, Paek KY, Choi K, Kim TD, Hahm B, Kim KT, Jang SK. (2003) Heterogeneous nuclear ribonucleoprotein C modulates translation of c-myc mRNA in a cell cycle phase-dependent manner. Mol Cell Biol. 23(2):708-720. | Synthesized sequences. | UV cross-linking, Immunoprecipitation, deletion assay | ||
hnRNP C | UCCCUUUUUUUUCCACAG | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP C | UCUUUCCUUCUUUUUUCCUUUCUUUUCCUUCCUUCUUUAAU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 10037806 | Gontarek RR, Gutshall LL, Herold KM, Tsai J, Sathe GM, Mao J, Prescott C, Del Vecchio AM. (1999) hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3'NTR of the HCV RNA genome. Nucleic Acids Res. 27(6):1457-1463. | HCV 3' NTR | UV cross-linking, immunoprecipitation, competition assay with HepG2 cell extract | ||
hnRNP C | AUCGCUUCUCGGCCUUUUAAGAUUCUAGA | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 8798770 | Temsamani J, Pederson T. (1996) The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle. J Biol Chem. 271(40): 24922-24926. | hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. | Sequences of U2 snRNA [6066] and homopolymers | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
hnRNP C | AUCGCUUCUCGGCCAAAAGGCUAAGAUCA | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 8798770 | Temsamani J, Pederson T. (1996) The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle. J Biol Chem. 271(40): 24922-24926. | hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. | Sequences of U2 snRNA [6066] and homopolymers | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
hnRNP C | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP C | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP C | CCCUUUUUUUUCCACAG | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP C | CCUUCUUCUUUUUCCUACAG | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP C1 | UUUUUU | -10 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 8083209 | Gorlach M, Burd CG, Dreyfuss G. (1994) The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. J Biol Chem. 269(37): 23074-8. | hnRNP C1 has no affinity to poly(rC). | Sequences of 20 nt random for SELEX. | SELEX of 20nt random sequences with purified protein confirmed by filter binding assay. | |
hnRNP C1 | UUUUUA | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 8083209 | Gorlach M, Burd CG, Dreyfuss G. (1994) The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. J Biol Chem. 269(37): 23074-8. | hnRNP C1 has no affinity to poly(rC). | Sequences of 20 nt random for SELEX. | SELEX of 20nt random sequences with purified protein confirmed by filter binding assay. | |
hnRNP C1 | UUUUUC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 8083209 | Gorlach M, Burd CG, Dreyfuss G. (1994) The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. J Biol Chem. 269(37): 23074-8. | hnRNP C1 has no affinity to poly(rC). | Sequences of 20 nt random for SELEX. | SELEX of 20nt random sequences with purified protein confirmed by filter binding assay. | |
hnRNP C1 | UUUUUG | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 8083209 | Gorlach M, Burd CG, Dreyfuss G. (1994) The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. J Biol Chem. 269(37): 23074-8. | hnRNP C1 has no affinity to poly(rC). | Sequences of 20 nt random for SELEX. | SELEX of 20nt random sequences with purified protein confirmed by filter binding assay. | |
hnRNP C1 | UUUUUUU | -7 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP C1 | UUUUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 11172920 | Sokolowski M, Schwartz S. (2001) Heterogeneous nuclear ribonucleoprotein C binds exclusively to the functionally important UUUUU-motifs in the human papillomavirus type-1 AU-rich inhibitory element. Virus Res. 73(2):163-175 | Sequence of Papillomavirus HPV-1 AU-rich element (h1ARE). | UV crosslink and SDS-PAGE with recombinant protein, competition assay. | ||
hnRNP C1 | GGGGGGG | -4 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 7688265 | Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. (1993) The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein. Cell. 74(2):291-298. | Homopolymers | Homopolymer binding assay with recombinant protein | ||
hnRNP C1 | UGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
hnRNP C1 | UGAACUUUAUAGUUGUUUUAUAUGUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
hnRNP C1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP C1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 Isoform C1 is due to Alternative Splicing. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP C2 | UUUUUUU | -7 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP C2 | UUUUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 9393875 | Sokolowski M, Zhao C, Tan W, Schwartz S. (1997) AU-rich mRNA instability elements on human papillomavirus type 1 late mRNAs and c-fos mRNAs interact with the same cellular factors. Oncogene. 15(19):2303-2319. | hnRNP C1 and C2 do not bind AUUUA motifs. | Sequences and mutants of 3' UTR HPV-1 late mRNA. | EMSA, UV crosslink, Western blot and immunoprecipitation with HeLa nuclear extracts. | |
hnRNP C2 | GGAUAC | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP C2 | GCAUAC | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP C2 | GGAUUC | -4 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP C2 | GGGUAC | -7 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP C2 | GGAUAU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP C2 | UUUUUUUUUUUUUUUUUUUUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 16157593 | Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005) TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing. J Biol Chem. 280(45):37572-37584. | Synthesized sequences | EMSA with recombinant protein | ||
hnRNP C2 | UGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
hnRNP C2 | UGAACUUUAUAGUUGUUUUAUAUGUU | -5 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
hnRNP C2 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP C2 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677 isoform C2 is due to Alternative Splicing. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP D | AUUUA | -1 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | UUAGGG | -8 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP D | UUAGGA | -6 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP D | UUAGAG | -4 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP D | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP D | AUAAAAGAACUUUUUUAUGCUUACCAUCUUUUUUUUUUCUUUAACAGAUUUGUAUUUAAGAAUUGUUUUUAAAAAAUUUUAAGAUUUACACAAUGUUUCUCUGUAAAUA | -9 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | UUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU | -10 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | AUUUAUUUAUUUAUUUAUUUA | -8 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | AUUUAUUUAUUUAUUUA | -6 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | AUUUAUUUAUUUA | -3 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | AUUUAUUUA | -2 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 8647811 | DeMaria CT, Brewer G. (1996) AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation. J Biol Chem. 271(21): 12179-84. | hnRNP D has no affinity to poly(rU). | WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] | UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein. | |
hnRNP D | GUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAG | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 11514570 | Wilson GM, Sutphen K, Moutafis M, Sinha S, Brewer G. (2001) Structural remodeling of an A + U-rich RNA element by cation or AUF1 binding. J Biol Chem. 276(42):38400-38409. | Synthesized sequences | Resonance energy transfer (RET) | ||
hnRNP D | GAUCUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUA | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 11514570 | Wilson GM, Sutphen K, Moutafis M, Sinha S, Brewer G. (2001) Structural remodeling of an A + U-rich RNA element by cation or AUF1 binding. J Biol Chem. 276(42):38400-38409. | Synthesized sequences | Resonance energy transfer (RET) | ||
hnRNP D | UUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 11719186 | Chen CY, Gherzi R, Ong SE, Chan EL, Raijmakers R, Pruijn GJ, Stoecklin G, Moroni C, Mann M, Karin M. (2001) AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell. 107(4):451-464. | Sequences deriving from FOS [2353] | UV crosslinking, imunoprecipitation with Jurkat extracts | ||
hnRNP D | UCAGCUAUUUACUGCCAAAGGGAAAUAUCAUUUAUUUUUUACAUUAUUAAGAAAAAAAGAUUUAUUUAUUUAAGACAGUCCCAUCAAAACUCCUGUCUUUGGAAAUC | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 11856759 | Lapucci A, Donnini M, Papucci L, Witort E, Tempestini A, Bevilacqua A, Nicolin A, Brewer G, Schiavone N, Capaccioli S. (2002) AUF1 Is a bcl-2 A+U-rich element-binding protein involved in bcl-2 mRNA destabilization during apoptosis. J Biol Chem. 277(18):16139-16146. | Sequences deriving from BCL2 [596] | EMSA supershift in Jurkat cytoplasmic extracts | ||
hnRNP D | GUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAC | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 12819194 | Wilson GM, Lu J, Sutphen K, Suarez Y, Sinha S, Brewer B, Villanueva-Feliciano EC, Ysla RM, Charles S, Brewer G. (2003) Phosphorylation of p40AUF1 regulates binding to A+U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure. J Biol Chem. 278(35):33039-33048. | Sequences deriving from TNF [7124] | EMSA with recombinant protein | ||
hnRNP D | UUUUUUAAAGUUUCUUGCAUUUAUUAUUCUCAAAAGUUUUUUCUAAGUUAAACAGUCAGUAUGCAAUCUUAAUAUAUGCUUUCUUUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 16289864 | Sommer S, Cui Y, Brewer G, Fuqua SA. (2005) The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 97(3):219-229. | Sequences deriving from YES1 [7525] | EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts. | ||
hnRNP D | UUUUUAUGUAAAACAUUUUUAGAACUCCAGUUUUCAAAUCAUGUUUGAAUCUACAUUCACUUUUUUUUGUUUUCUUUUUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 16289864 | Sommer S, Cui Y, Brewer G, Fuqua SA. (2005) The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 97(3):219-229. | Sequences deriving from YES1 [7525] | EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts. | ||
hnRNP D | UGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
hnRNP D | UGAACUUUAUAGUUGUUUUAUAUGUU | -10 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
hnRNP D | AAAAAAA | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 16834569 | Sagliocco F, Laloo B, Cosson B, Laborde L, Castroviejo M, Rosenbaum J, Ripoche J, Grosset C. (2006) The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP. Biochem J. 400(2):337-347. | Synthesized oligos | Homopolymer binding assay and Western blot with HeLa extracts or recombinant protein. | ||
hnRNP D | AGACUUUAUGUAGUUUUUAUAUGUUGUAAUAUUUCUUCAAAUAAAUCUCUCCUAUAA | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 17030625 | Torrisani J, Unterberger A, Tendulkar SR, Shikimi K, Szyf M. (2007) AUF1 cell cycle variations define genomic DNA methylation by regulation of DNMT1 mRNA stability. Mol Cell Biol. 27(1):395-410. | Sequences deriving from DNMT1 [1786]. Synthesized sequences. | UV cross-linking in HeLa cytoplasmic extracts, MALDI-TOF, Western blot | ||
hnRNP D | CAUAAAUUAUUUUCAAGUGUAACUUAUUAACCUAUUUAUUAUUUAUGUAUUUAUUUAAGC | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 18650551 | Palanisamy V, Park NJ, Wang J, Wong DT. (2008) AUF1 and HuR proteins stabilize interleukin-8 mRNA in human saliva. J Dent Res. 87(8):772-776. | Sequences deriving from IL8 [3576] | UV cross-linking, immunoprecipitation with salivary protein extracts. | ||
hnRNP D | CCAUUUAUAUCAUUUUUUAUAUAUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
hnRNP D | CCAUUUAUAUCAUAAAAUAUAUAUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
hnRNP D | CCAUUUAUAUCAUUUUUAAAAAAUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
hnRNP D | CCAUUUAUAUCAUAAAAAUAUUAUU | -5 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
hnRNP D | AGAUAU | -9 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP D | AAUUUA | -9 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP D | AGUAGG | -9 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP D0 | UUAGGG | -6 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD Isoform:Isoform D0 is due to Alternative Splicing. | 7673195 | Kajita Y, Nakayama J, Aizawa M, Ishikawa F. (1995) The UUAG-specific RNA binding protein, heterogeneous nuclear ribonucleoprotein D0. Common modular structure and binding properties of the 2xRBD-Gly family. J Biol Chem. 270(38): 22167-22175. | Synthesized sequences | Filter Binding Assay of recombinant protein and synthesized oligos. | ||
hnRNP D0 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD Isoform:Isoform D0 is due to Alternative Splicing. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP D0 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD Isoform:Isoform D0 is due to Alternative Splicing. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP DL | AACCUUGCC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | AAUACCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACACCAGAC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACAUUAGCC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACCACGCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUACAGCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAACU | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAAGUA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAGAG | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAGCC | -10 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAGCG | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAGCU | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUAGGA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUGCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | ACUUUAA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | AGUAGCC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | AUCUGAC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | AUGCGCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | AUUAGGCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | CCUUUAGGC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GAACUAAGC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GACUAGCA | -5 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GACUAGCG | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GCACUAGAU | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GCACUAGGC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GCGAGCA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GCUAGUA | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GGACUAGCC | -5 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GGACUAGCU | -3 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GGAGUAGCC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GGAGUAGCU | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | GGAUUAGCC | -7 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | UGUCGC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 12406575 | Kamei D, Yamada M. (2003) Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites. Gene 298(1):49-57. | Sequences of 20 nt random for SELEX. | SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells. | ||
hnRNP DL | UUUAGUCAGCCUUAUAGCUAA | -5 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
hnRNP DL | AGAUAU | -7 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP DL | AAUUUA | -9 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP DL | AGUAGG | -9 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 24452013 | Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014) The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration. Nat Commun. 5:3078. | Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin. | In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins. | RNA affinity chromatography and SPR analysis with recombinant protein. | |
hnRNP DL | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP DL | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP E1 | UUAGGG | -8 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP E1 | UUAGGA | -6 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP E1 | UUAGAG | -4 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP E1 | CCCCCCC | -7 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 7607214 | Leffers H, Dejgaard K, Celis JE. (1995) Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains. Eur J Biochem. 230(2): 447-453. | hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). | homopolymers | Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers. | |
hnRNP E1 | GGGGGGG | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP E1 | UUUUUUU | -4 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 7607214 | Leffers H, Dejgaard K, Celis JE. (1995) Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains. Eur J Biochem. 230(2): 447-453. | hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). | homopolymers | Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers. | |
hnRNP E1 | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP E1 | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU | -2 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP E1 | CCCAACGGGCCCUCCUCCCC | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 9122208 | Holcik M, Liebhaber SA. (1997) Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components. Proc Natl Acad Sci U S A. 94(6):2410-2414. | Synthesized sequences. | RNase H mapping, EMSA, immunoblot | ||
hnRNP E1 | CCCAGCCGGCCCUCCUCCCC | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 9122208 | Holcik M, Liebhaber SA. (1997) Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components. Proc Natl Acad Sci U S A. 94(6):2410-2414. | Synthesized sequences. | RNase H mapping, EMSA, immunoblot | ||
hnRNP E1 | CCCCACGGGCCCUCCUCCCC | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 9122208 | Holcik M, Liebhaber SA. (1997) Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components. Proc Natl Acad Sci U S A. 94(6):2410-2414. | Synthesized sequences. | RNase H mapping, EMSA, immunoblot | ||
hnRNP E1 | CCCCACCCUCUUCCCCAAGCCCCACCCUCUUCCCCAAG | -4 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 9160751 | Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M, Hentze MW. (1997) mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end. Cell. 16 | Constructs of luciferase gene reporter. | In vivo splicing assay with luciferase gene reporter in HeLa | UV cross-linking. Cell-free translation of different mRNAs. | |
hnRNP E1 | CCCUCCC | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 12011088 | Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002) Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA. J Biol Chem. 277(30):27183-27192. | Sequences deriving from AR [367]. Synthesized oligos. | Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts | ||
hnRNP E1 | CCCUCUGCCACCCAGGCAGGCCCUGCCUUCAGCCCUGGCCCAGAGCUGGAACACUCUCUGAGAUGCCCCUCUGCCUGGGCUUAUGCCCUCAGAUGGAGACAUUGGAUGUGGAGCUCCUGCUGGAUGCGUGCCCUGACCCCUGCACCAGCCCUUCCCUGCUUUGAG | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 12600897 | Skalweit A, Doller A, Huth A, Kahne T, Persson PB, Thiele BJ. (2003) Posttranscriptional control of renin synthesis: identification of proteins interacting with renin mRNA 3'-untranslated region. Circ Res. 92(4):419-427. | Sequences deriving from REN [5972] | EMSA, UV cross-linking with Calu-6 cell extracts. RNA-affinity chromatography with MALDI-TOF-MS. Western blot. | ||
hnRNP E1 | CCCAACCUGGCUCCCUCCCACCCAACCAACUUUCCCCCCAACCC | -10 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 15514164 | Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004) RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res. 95(11):1058-1066. | Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] | EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF. | ||
hnRNP E1 | CUCCUUCCCCCGCUCCCCCAA | -4 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 15514164 | Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004) RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res. 95(11):1058-1066. | Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] | EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF. | ||
hnRNP E1 | GCCGCUCCAGCGCCGCGCAGCCACCGCCGCCGC | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 15967798 | Thomson AM, Cahill CM, Cho HH, Kassachau KD, Epis MR, Bridges KR, Leedman PJ, Rogers JT. (2005) The acute box cis-element in human heavy ferritin mRNA 5'-untranslated region is a unique translation enhancer that binds poly(C)-binding proteins. J Biol Chem. 280(34):30032-30045. | Sequences deriving from FTH1 [2495] | EMSA, UV cross-linking, western blot with HepG2 cytoplasmic extract or recombinant protein | ||
hnRNP E1 | UCCCCAA | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 16854432 | Messias AC, Harnisch C, Ostareck-Lederer A, Sattler M, Ostareck DH. (2006) The DICE-binding activity of KH domain 3 of hnRNP K is affected by c-Src-mediated tyrosine phosphorylation. J Mol Biol. 361(3):470-481. | Synthesized sequences | UV cross-linking, Chemical shift mapping (NMR) with recombinant protein. | ||
hnRNP E1 | AAAAAAA | -2 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 8917439 | Dejgaard K, Leffers H. (1996) Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains. Eur J Biochem. 241(2):425-431. | Synthesized oligos | Homopolymer binding assay with recombinant protein. | ||
hnRNP E1 | CCCUUCCC | -10 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | AAAUUCCC | -10 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | CCCUUAAA | -10 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | ACAUUAAA | -1 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | CAAUUAAA | -2 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | CCAUUAAA | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | ACCUUAAA | -7 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | AAACCAAA | -9 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | AAAUUCCA | -10 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | AAAUUACC | -9 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | AAAUUCAC | -3 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | CUCUGGGGUUGUACCCACCCCAGAG | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E1 | AGCCACCUCCCACCCCACCCCA | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17928403 | Lee PT, Liao PC, Chang WC, Tseng JT. (2007) Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 18(12):5004-5013. | Sequences deriving from mutated GAST [2520] and homopolymers. | EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts. | ||
hnRNP E1 | CCCAGCCCUGUCCCCUGAAAAACUGA | -5 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 17928403 | Lee PT, Liao PC, Chang WC, Tseng JT. (2007) Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 18(12):5004-5013. | Sequences deriving from mutated GAST [2520] and homopolymers. | EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts. | ||
hnRNP E1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP E1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP E2 | UUAGGG | -8 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP E2 | UUAGGA | -6 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP E2 | UUAGAG | -4 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 8321232 | Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993) Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n. Mol Cell Biol. 13(7): 4301-4310. | synthesized oligos | EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos. | ||
hnRNP E2 | CCCCCCC | -7 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 7607214 | Leffers H, Dejgaard K, Celis JE. (1995) Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains. Eur J Biochem. 230(2): 447-453. | hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). | homopolymers | Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers. | |
hnRNP E2 | GGGGGGG | -7 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP E2 | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP E2 | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU | -2 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP E2 | CCCAACGGGCCCUCCUCCCC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 9122208 | Holcik M, Liebhaber SA. (1997) Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components. Proc Natl Acad Sci U S A. 94(6):2410-2414. | Synthesized sequences. | RNase H mapping, EMSA, immunoblot | ||
hnRNP E2 | CCCAGCCGGCCCUCCUCCCC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 9122208 | Holcik M, Liebhaber SA. (1997) Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components. Proc Natl Acad Sci U S A. 94(6):2410-2414. | Synthesized sequences. | RNase H mapping, EMSA, immunoblot | ||
hnRNP E2 | CCCCACGGGCCCUCCUCCCC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 9122208 | Holcik M, Liebhaber SA. (1997) Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components. Proc Natl Acad Sci U S A. 94(6):2410-2414. | Synthesized sequences. | RNase H mapping, EMSA, immunoblot | ||
hnRNP E2 | CUCGCCAUGCCGGGAGAACUCUAACUCCCCCAUGGAG | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 11753385 | Perrotti D, Cesi V, Trotta R, Guerzoni C, Santilli G, Campbell K, Iervolino A, Condorelli F, Gambacorti-Passerini C, Caligiuri MA, Calabretta B. (2002) BCR-ABL suppresses C/EBPalpha expression through inhibitory action of hnRNP E2. Nat Genet. 30(1):48-58. | Sequences deriving from CEBPA [1050] | RNA affinity chromatography and EMSA supershift in K562 cytoplasmic extracts. Protein sequencing. | ||
hnRNP E2 | CCCUCCC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 12011088 | Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002) Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA. J Biol Chem. 277(30):27183-27192. | Sequences deriving from AR [367]. Synthesized oligos. | Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts | ||
hnRNP E2 | UUUUUUU | -7 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 12011088 | Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002) Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA. J Biol Chem. 277(30):27183-27192. | Sequences deriving from AR [367]. Synthesized oligos. | Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts | ||
hnRNP E2 | UCCCCA | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 15331611 | Du Z, Yu J, Chen Y, Andino R, James TL. (2004) Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2. J Biol Chem. 279(46):48126-48134. | Synthesized sequences and HPV-1 IRES. | NMR using recombinant protein | ||
hnRNP E2 | CUAACCCUAA | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 15331611 | Du Z, Yu J, Chen Y, Andino R, James TL. (2004) Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2. J Biol Chem. 279(46):48126-48134. | Synthesized sequences and HPV-1 IRES. | NMR using recombinant protein | ||
hnRNP E2 | GGAGGACAUCCCUCACGGGUGACCGUGGUCCUCC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 15331611 | Du Z, Yu J, Chen Y, Andino R, James TL. (2004) Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2. J Biol Chem. 279(46):48126-48134. | Synthesized sequences and HPV-1 IRES. | NMR using recombinant protein | ||
hnRNP E2 | GGGGUUGUACCCACCCCAC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 15331611 | Du Z, Yu J, Chen Y, Andino R, James TL. (2004) Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2. J Biol Chem. 279(46):48126-48134. | Synthesized sequences and HPV-1 IRES. | NMR using recombinant protein | ||
hnRNP E2 | GCCGCUCCAGCGCCGCGCAGCCACCGCCGCCGC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 15967798 | Thomson AM, Cahill CM, Cho HH, Kassachau KD, Epis MR, Bridges KR, Leedman PJ, Rogers JT. (2005) The acute box cis-element in human heavy ferritin mRNA 5'-untranslated region is a unique translation enhancer that binds poly(C)-binding proteins. J Biol Chem. 280(34):30032-30045. | Sequences deriving from FTH1 [2495] | EMSA, UV cross-linking, western blot with HepG2 cytoplasmic extract or recombinant protein | ||
hnRNP E2 | CCAUUC | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17451601 | Woolaway K, Asai K, Emili A, Cochrane A. (2007) hnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expression. Retrovirology. 4:28. | Sequences deriving from HIV-1 Tat [155871] | Protein affinity purification with HeLa NE, mass spectrometry, western blot. | ||
hnRNP E2 | CCCUUCCC | -10 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | AAAUUCCC | -10 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | CCCUUAAA | -10 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | ACAUUAAA | -1 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | CAAUUAAA | -2 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | CCAUUAAA | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | ACCUUAAA | -7 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | AAACCAAA | -9 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | AAAUUCCA | -10 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | AAAUUACC | -9 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | AAAUUCAC | -3 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | CUCUGGGGUUGUACCCACCCCAGAG | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 17609276 | Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007) Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site. J Virol. 81(18):10017-10028. | Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. | Pull-down assay with recombinant protein and HeLa cell extracts. | ||
hnRNP E2 | CGCUCAGCACUACCCCAGUUGAGCU | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 18929541 | Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Gorlach M. (2008) Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA. Biochem Biophys Res Commun. 377(2):500-503. | Sequences deriving from CVB3 5' UTR | EMSA with recombinant protein | ||
hnRNP E2 | CAGGUCGAUGAGUCACCGCAUUCCCCACGGGCGACCGUGGCGGUGGCUGCGUUGGCGGCCUG | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 18929541 | Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Gorlach M. (2008) Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA. Biochem Biophys Res Commun. 377(2):500-503. | Sequences deriving from CVB3 5' UTR | EMSA with recombinant protein | ||
hnRNP E2 | CCUGUGGGUUGAUCCCACCCACAGG | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 18929541 | Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Gorlach M. (2008) Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA. Biochem Biophys Res Commun. 377(2):500-503. | Sequences deriving from CVB3 5' UTR | EMSA with recombinant protein | ||
hnRNP E2 | CUCUGCCCUUCCGU | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 20211135 | Eiring AM, Harb JG, Neviani P, Garton C, Oaks JJ, Spizzo R, Liu S, Schwind S, Santhanam R, Hickey CJ, Becker H, Chandler JC, Andino R, Cortes J, Hokland P, Huettner CS, Bhatia R, Roy DC, Liebhaber SA, Caligiuri MA, Marcucci G, Garzon R, Croce CM, Calin GA, Perrotti D. (2010) miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts. Cell. 140(5):652-665. | Sequences deriving from MIR328 [442901] | EMSA, UV cross-linking and immunoprecipitation with human recombinant protein expressed in murine cells. | ||
hnRNP E2 | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP E2 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP E2 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP F | GGGGGGG | -7 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 7512260 | Matunis MJ, Xing J, Dreyfuss G. (1994) The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization. Nucleic Acids Res. 22(6): 1059-67. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP F | GGGGGCUG | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
hnRNP F | GGAGGA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP F | AAGGGGAA | 5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP F | AGGGA | -8 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP F | CGGGC | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AAGGUG | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP F | GGGGGAGGUGUGGG | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | AGGGGAGGUGUGGG | -6 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GAGGGAGGUGUGGG | -3 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGAGGAGGUGUGGG | -1 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGAGAGGUGUGGG | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGAAGGUGUGGG | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAAGUGUGGG | -3 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGAUGUGGG | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGGUAUGGG | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGGUGUAGG | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGGUGUGAG | -10 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGGUGUGGA | -7 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGGUGUGAA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAGGUGUAAG | -3 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGGGAAAUGUGGG | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGGAAAGGUGUGGG | -3 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GGAAGAGGUGUGGG | -1 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | GAAGGAGGUGUGGG | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | AAGGGAGGUGUGGG | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP F | CGAUGGGAA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | CGGGAUGGGGUA | -10 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | CUGGGGU | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | UGGGA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | UGGGGU | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 12228232 | Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002) Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene. J Biol Chem. 277(46):43821-43829. | Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9 | In vivo splicing in HeLa | Mutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting | |
hnRNP F | GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 11421362 | Hastings ML, Wilson CM, Munroe SH. (2001) A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA. RNA. 7(6):859-874. | Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing with HeLa NE | UV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins. | |
hnRNP F | AGGGUUGAGGGGAGCAGGGU | -7 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP F | CGGGUUGACGGGAGCACGGU | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP F | AGUGUUGAGUGGAGCAGUGGU | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP F | UGGGC | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGC | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGU | -6 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGG | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | GGGGG | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | CGGGGGGGGC | -6 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGGAGGGG | -6 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | CAGAAGGGGAGGGGUUCCA | -10 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP F | AACAAGGGGUGGGGGAAAA | -10 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP F | GGGGAAUAUACGUGCUUGGCGGGUAAUUCUAUUGGGA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 18573884 | Mauger DM, Lin C, Garcia-Blanco MA. (2008) hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. Mol Cell Biol. 28(17):5403-5419. | Constructs of FGFR2 [2263] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10. | In vivo splicing in HEK293 | Splicing assays with wt and mutant minigenes. siRNA. | |
hnRNP F | GGGAAUGUGGG | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 19506027 | Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009) A physical and functional link between splicing factors promotes pre-mRNA 3' end processing. Nucleic Acids Res. 37(14):4672-4683. | Sequences deriving from HBB [3043] | UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein | ||
hnRNP F | AAGGCGAA | 1 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP F | CGGGA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP F | GGGGA | -4 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | UGGGG | -3 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | GGGGU | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 16885237 | Dominguez C, Allain FH. (2006) NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition. Nucleic Acids Res. 34(13):3634-3645. | Synthesized sequences. | NMR titration using recombinant protein | ||
hnRNP F | GGGGC | -2 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 12228232 | Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002) Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene. J Biol Chem. 277(46):43821-43829. | Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9 | In vivo splicing in HeLa | Mutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting | |
hnRNP F | AGGGAU | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 20526337 | Dominguez C, Fisette JF, Chabot B, Allain FH. (2010) Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs. Nat Struct Mol Biol. 17(7):853-861. | Synthesized sequences. Constructs of wt and mutant BCL2L1 [598] EX2 - INT2 - EX3. | In vitro splicing assays in HeLa nuclear extracts. | NMR spectroscopy. In vitro splicing assays in HeLa nuclear extracts with increasing amount of splicing factor. | |
hnRNP F | GGGAUGGGGUAAACUGGGG | 5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 20526337 | Dominguez C, Fisette JF, Chabot B, Allain FH. (2010) Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs. Nat Struct Mol Biol. 17(7):853-861. | Synthesized sequences. Constructs of wt and mutant BCL2L1 [598] EX2 - INT2 - EX3. | In vitro splicing assays in HeLa nuclear extracts. | NMR spectroscopy. In vitro splicing assays in HeLa nuclear extracts with increasing amount of splicing factor. | |
hnRNP F | GGAGGGUUAGGGUUAGGGUUAGGGUUA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 23275549 | Samatanga B, Dominguez C, Jelesarov I, Allain FH. (2013) The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA. Nucleic Acids Res. 41(4):2505-2516. | Synthesized sequences | NMR spectroscopy, isothermal titration calorimetry | ||
hnRNP F | GAGUUAGGGUUAGGGUUAGGGUUAGGGUUA | -5 | Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. | 23275549 | Samatanga B, Dominguez C, Jelesarov I, Allain FH. (2013) The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA. Nucleic Acids Res. 41(4):2505-2516. | Synthesized sequences | NMR spectroscopy, isothermal titration calorimetry | ||
hnRNP G | GGGUACGACGGAUAUCGUGGGGGGGGAAAUUGCUUUCGGUUCCGACUCUG | -5 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 21327109 | Kanhoush R, Beenders B, Perrin C, Moreau J, Bellini M, Penrad-Mobayed M. (2010) Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts. Nucleus. 1(1):109-122. | Synthesized sequences | 1D- and 2D-Northwestern assays, Westernblot with HeLa NE | ||
hnRNP G | AUCAAA | 5 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | UAAGAC | 5 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | ACCAAA | 5 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | UCAAAA | 5 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | AAAAU | 5 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | AUCCCA | 1 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | AUCCCC | 1 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
hnRNP G | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP G | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP H1 | CGGGA | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
hnRNP H1 | AUUGGGUGU | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11526107 | Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001) A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J Biol Chem. 276(44): 40464-75. | Constructs of HIV-1 Tat [155871] EX2. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | EMSA, UV crosslink, immunoprecipitation and immunoblot. | |
hnRNP H1 | GGGGGGG | -7 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 7512260 | Matunis MJ, Xing J, Dreyfuss G. (1994) The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization. Nucleic Acids Res. 22(6): 1059-67. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP H1 | GGGGGCUG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
hnRNP H1 | UGUGGG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 10072387 | Chen CD, Kobayashi R, Helfman DM. (1999) Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene. Genes Dev. 13(5):593-606. | Construct of rat beta-tropomyosin Tpm2 [500450] EX5-INT5-EX7 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assays, immunoblot, immunodepletion in HeLa nuclear extracts. | |
hnRNP H1 | GGAGGA | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP H1 | CGAAUCGACAAAGGGGAGGAAGUGGGAGAAA | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 10934202 | Fogel BL, McNally MT. (2000) A cellular protein, hnRNP H, binds to the negative regulator of splicing element from Rous sarcoma virus. J Biol Chem. 275(41):32371-32378. | Sequence and variants of NRS5' region Rous sarcoma virus. | UV crosslink in HeLa nuclear extracts. RNA affinity selection with HeLa extracts. Immunoprecipitation, EMSA supershift. | ||
hnRNP H1 | AAGGGGAA | 5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP H1 | AGGGA | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP H1 | CGGGC | -6 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AUCGGGCGU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11526107 | Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001) A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J Biol Chem. 276(44): 40464-75. | Constructs of HIV-1 Tat [155871] EX2. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | EMSA, UV crosslink, immunoprecipitation and immunoblot. | |
hnRNP H1 | AAGGUG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP H1 | UGGGC | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | UGGGG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H1 | GAUCACUGGGGUGGAUCAUCCAGGUGGGGCUUUU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16396608 | Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006) Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. PLoS Biol. 4(2):e21. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Immunoprecipitation, Gel shift and RNase H protection assays | |
hnRNP H1 | GGGUCGAGGGG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16396608 | Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006) Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. PLoS Biol. 4(2):e21. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Immunoprecipitation, Gel shift and RNase H protection assays | |
hnRNP H1 | GAUCACUGGGGUGGAUCAU | -1 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H1 | GAUCACUGUGGUGGAUCAUCCAGGUGUGGCUUUU | -1 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H1 | GAUCACUGUGGUGGAUCAUCCAGGUGGGGCUUUU | -1 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H1 | CCAUGGUUUGGGAGUGGGAAGGUGGGGAG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H1 | CCAUGGUUUGGGGGCAGUAGUUGG | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H1 | UGGGGU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 12228232 | Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002) Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene. J Biol Chem. 277(46):43821-43829. | Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9 | In vivo splicing in HeLa | Mutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting | |
hnRNP H1 | GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC | -10 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11421362 | Hastings ML, Wilson CM, Munroe SH. (2001) A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA. RNA. 7(6):859-874. | Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing with HeLa NE | UV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins. | |
hnRNP H1 | AGGGUUGAGGGGAGCAGGGU | -7 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H1 | CGGGUUGACGGGAGCACGGU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H1 | AGUGUUGAGUGGAGCAGUGGU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H1 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP H1 | AUACCUUUUUGGGGAGGGGGCAGAGAGC | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16254078 | Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005) Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene. Nucleic Acids Res. 33(18):6000-6010. | Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3 | In vivo splicing in Hep3B | Pull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA. | |
hnRNP H1 | AAGAA | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | CAGGAC | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGAGA | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGAGG | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGAG | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGC | -6 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGU | -6 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGG | -6 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | GGGGG | -6 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | CGGGGGGGGC | -6 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGGAGGGG | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | CAGAAGGGGAGGGGUUCCA | -3 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP H1 | AACAAGGGGUGGGGGAAAA | -3 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP H1 | GUAUCCUUCCCUGGCGGGGGUGGGAGAGCAGCAGGGCCAGGGGGGUGACACACC | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGGGAUGGGCGAGAUGGGGUGCUGU | -10 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGGGAUGGGCGAGAUGGGGUGCUGU | -4 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGUGAUGUGCGAGAUGGUGUGCUGU | -8 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGUGAUGUGCGAGAUGGUGUGCUGU | -1 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | GGUUGGGAGAGGGAUUUC | -10 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | GGUUGUGAGAGUGAUUUC | -1 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H1 | GGGGAAUAUACGUGCUUGGCGGGUAAUUCUAUUGGGA | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 18573884 | Mauger DM, Lin C, Garcia-Blanco MA. (2008) hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc. Mol Cell Biol. 28(17):5403-5419. | Constructs of FGFR2 [2263] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10. | In vivo splicing in HEK293 | Splicing assays with wt and mutant minigenes. siRNA. | |
hnRNP H1 | GGGAAUGUGGG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 19506027 | Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009) A physical and functional link between splicing factors promotes pre-mRNA 3' end processing. Nucleic Acids Res. 37(14):4672-4683. | Sequences deriving from HBB [3043] | UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein | ||
hnRNP H1 | GGGGU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H1 | UGGGA | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H1 | UGGGU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 20100605 | Russo A, Siciliano G, Catillo M, Giangrande C, Amoresano A, Pucci P, Pietropaolo C, Russo G. (2010) hnRNP H1 and intronic G runs in the splicing control of the human rpL3 gene. Biochim Biophys Acta. 1799(5-6):419-428. | Construct of RPL3 [6122] EX3 - INT3 - EX4 | In vivo splicing in HeLa | GST pull-down, mass spectrometry, Immunoprecipitation, western blotting, RNA pull-down assay, REMSA, RNA interference in HeLa and Calu6 | |
hnRNP H1 | CUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | CUGAGGCUGGGGGCUGGAAUUCCCAUGUGCUUCCU | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | UAUGACCCGGACUCCCGGUG | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | UAUGAUAGGCACUUAGGCUG | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | CUGGGGGCUG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | GGGGC | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | AAGGCGAA | 1 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP H1 | CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H1 | GGGGA | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 24290757 | Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V, Miller JW, Smith BN, Gallo JM, Ule J, Hirth F, Rogelj B, Houart C, Shaw CE. (2013) Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic. Cell Rep. 5(5):1178-1186. | Synthetic sequences. | RNA pull-down assay using SH-SY5Y cell extract. | ||
hnRNP H1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP H1 | UGGGACUGGGACUGGGACUG | -5 | Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP H2 | CGGGA | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
hnRNP H2 | AUUGGGUGU | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11526107 | Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001) A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J Biol Chem. 276(44): 40464-75. | Constructs of HIV-1 Tat [155871] EX2. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | EMSA, UV crosslink, immunoprecipitation and immunoblot. | |
hnRNP H2 | GGGGGGG | -7 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 7512260 | Matunis MJ, Xing J, Dreyfuss G. (1994) The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization. Nucleic Acids Res. 22(6): 1059-67. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP H2 | GGGGGCUG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
hnRNP H2 | UGUGGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 10072387 | Chen CD, Kobayashi R, Helfman DM. (1999) Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene. Genes Dev. 13(5):593-606. | Construct of rat beta-tropomyosin Tpm2 [500450] EX5-INT5-EX7 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assays, immunoblot, immunodepletion in HeLa nuclear extracts. | |
hnRNP H2 | GGAGGA | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP H2 | CGAAUCGACAAAGGGGAGGAAGUGGGAGAAA | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 10934202 | Fogel BL, McNally MT. (2000) A cellular protein, hnRNP H, binds to the negative regulator of splicing element from Rous sarcoma virus. J Biol Chem. 275(41):32371-32378. | Sequence and variants of NRS5' region Rous sarcoma virus. | UV crosslink in HeLa nuclear extracts. RNA affinity selection with HeLa extracts. Immunoprecipitation, EMSA supershift. | ||
hnRNP H2 | AAGGGGAA | 5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP H2 | AGGGA | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19404736 | Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009) The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding. Breast Cancer Res Treat. 120(2):391-399. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates. | In vitro splicing in HeLa nuclear extract | Mutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract. | |
hnRNP H2 | CGGGC | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AUCGGGCGU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11526107 | Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001) A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J Biol Chem. 276(44): 40464-75. | Constructs of HIV-1 Tat [155871] EX2. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | EMSA, UV crosslink, immunoprecipitation and immunoblot. | |
hnRNP H2 | AAGGUG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP H2 | UGGGC | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | UGGGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H2 | GGGGGAGGUGUGGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | AGGGGAGGUGUGGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GAGGGAGGUGUGGG | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGAGGAGGUGUGGG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGAGAGGUGUGGG | -4 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGAAGGUGUGGG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAAGUGUGGG | -3 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGAUGUGGG | -4 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGGUAUGGG | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGGUGUAGG | -4 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGGUGUGAG | -10 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGGUGUGGA | -10 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGGUGUGAA | -4 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAGGUGUAAG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGGGAAAUGUGGG | -7 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGGAAAGGUGUGGG | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGAAGAGGUGUGGG | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GAAGGAGGUGUGGG | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | AAGGGAGGUGUGGG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GGAGAAGGUGUGGG | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16171461 | Alkan SA, Martincic K, Milcarek C. (2006) The hnRNPs F and H2 bind to similar sequences to influence gene expression. Biochem J. 393(Pt 1):361-371. | SVL (SV40 late) pre-mRNA and mutants | UV cross-linking, EMSA, filter binding assays using recombinant protein | ||
hnRNP H2 | GAUCACUGGGGUGGAUCAUCCAGGUGGGGCUUUU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16396608 | Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006) Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. PLoS Biol. 4(2):e21. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Immunoprecipitation, Gel shift and RNase H protection assays | |
hnRNP H2 | GGGUCGAGGGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16396608 | Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006) Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. PLoS Biol. 4(2):e21. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Immunoprecipitation, Gel shift and RNase H protection assays | |
hnRNP H2 | GAUCACUGGGGUGGAUCAU | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H2 | GAUCACUGUGGUGGAUCAUCCAGGUGUGGCUUUU | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H2 | GAUCACUGUGGUGGAUCAUCCAGGUGGGGCUUUU | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H2 | CCAUGGUUUGGGAGUGGGAAGGUGGGGAG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H2 | CCAUGGUUUGGGGGCAGUAGUUGG | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H2 | UGGGGU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 12228232 | Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002) Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene. J Biol Chem. 277(46):43821-43829. | Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9 | In vivo splicing in HeLa | Mutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting | |
hnRNP H2 | GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC | -10 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11421362 | Hastings ML, Wilson CM, Munroe SH. (2001) A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA. RNA. 7(6):859-874. | Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing with HeLa NE | UV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins. | |
hnRNP H2 | AGGGUUGAGGGGAGCAGGGU | -7 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H2 | CGGGUUGACGGGAGCACGGU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H2 | AGUGUUGAGUGGAGCAGUGGU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H2 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP H2 | AAGAA | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | CAGGAC | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGAGA | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGAGG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGAG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGC | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGU | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGG | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | GGGGG | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | CGGGGGGGGC | -6 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGGAGGGG | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | GUAUCCUUCCCUGGCGGGGGUGGGAGAGCAGCAGGGCCAGGGGGGUGACACACC | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGGGAUGGGCGAGAUGGGGUGCUGU | -10 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGGGAUGGGCGAGAUGGGGUGCUGU | -4 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGUGAUGUGCGAGAUGGUGUGCUGU | -8 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGUGAUGUGCGAGAUGGUGUGCUGU | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | GGUUGGGAGAGGGAUUUC | -10 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | GGUUGUGAGAGUGAUUUC | -1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H2 | GGGAAUGUGGG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 19506027 | Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009) A physical and functional link between splicing factors promotes pre-mRNA 3' end processing. Nucleic Acids Res. 37(14):4672-4683. | Sequences deriving from HBB [3043] | UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein | ||
hnRNP H2 | GGGGU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H2 | UGGGA | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H2 | CUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | CUGAGGCUGGGGGCUGGAAUUCCCAUGUGCUUCCU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | UAUGACCCGGACUCCCGGUG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | UAUGAUAGGCACUUAGGCUG | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | CUGGGGGCUG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | GGGGC | -2 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | AAGGCGAA | 1 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 15828859 | Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005) A combinatorial code for splicing silencing: UAGG and GGGG motifs. PLoS Biol. 3(5):e158. | In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. | Synthesized sequences based on GRIN1 [2902] EX19. | Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP H2 | UGGGU | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 18806275 | Masuda A, Shen XM, Ito M, Matsuura T, Engel AG, Ohno K. (2008) hnRNP H enhances skipping of a nonfunctional exon P3A in CHRNA1 and a mutation disrupting its binding causes congenital myasthenic syndrome. Hum Mol Genet. 17(24):4022-4035. | Construct of CHRNA1 [1134] EX2-INT2-EX3-INT-EX_P3A-INT-EX4 | In vivo splicing assay in HeLa | Western blotting using HEK293T nuclear extract, immunodepletion, surface plasmon resonance | |
hnRNP H2 | CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H2 | CAGAAGGGGAGGGGUUCCA | -3 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP H2 | AACAAGGGGUGGGGGAAAA | -3 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP H2 | GGGGA | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H2 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 24290757 | Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V, Miller JW, Smith BN, Gallo JM, Ule J, Hirth F, Rogelj B, Houart C, Shaw CE. (2013) Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic. Cell Rep. 5(5):1178-1186. | Synthetic sequences. | RNA pull-down assay using SH-SY5Y cell extract. | ||
hnRNP H2 | UGGGACUGGGACUGGGACUG | -5 | Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP H3 | AGGGC | -6 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGU | -6 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGA | -6 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGG | -6 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | GGGGG | -6 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | CGGGGGGGGC | -6 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGGAGGGG | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | UGGGC | -2 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | CGGGC | -2 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP H3 | GGGGGGG | -7 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP H3 | UGUGGG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 10072387 | Chen CD, Kobayashi R, Helfman DM. (1999) Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene. Genes Dev. 13(5):593-606. | Construct of rat beta-tropomyosin Tpm2 [500450] EX5-INT5-EX7 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assays, immunoblot, immunodepletion in HeLa nuclear extracts. | |
hnRNP H3 | GGGGGCUG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
hnRNP H3 | GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC | -10 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 11421362 | Hastings ML, Wilson CM, Munroe SH. (2001) A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA. RNA. 7(6):859-874. | Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing with HeLa NE | UV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins. | |
hnRNP H3 | AUUGGGUGU | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 11526107 | Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001) A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J Biol Chem. 276(44): 40464-75. | Constructs of HIV-1 Tat [155871] EX2. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | EMSA, UV crosslink, immunoprecipitation and immunoblot. | |
hnRNP H3 | AUCGGGCGU | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 11526107 | Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001) A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H. J Biol Chem. 276(44): 40464-75. | Constructs of HIV-1 Tat [155871] EX2. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | EMSA, UV crosslink, immunoprecipitation and immunoblot. | |
hnRNP H3 | CGGGA | 5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
hnRNP H3 | GGAGGA | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP H3 | AAGGUG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
hnRNP H3 | UGGGG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H3 | GGGGU | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H3 | UGGGA | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 12732620 | Pagani F, Buratti E, Stuani C, Baralle FE. (2003) Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9. J Biol Chem. 278(29):26580-26588. | Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in Hep3B cells of WT and mutant CFTR constructs. | UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs. | |
hnRNP H3 | AGGGUUGAGGGGAGCAGGGU | -7 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H3 | CGGGUUGACGGGAGCACGGU | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H3 | AGUGUUGAGUGGAGCAGUGGU | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
hnRNP H3 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP H3 | GUAUCCUUCCCUGGCGGGGGUGGGAGAGCAGCAGGGCCAGGGGGGUGACACACC | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGGGAUGGGCGAGAUGGGGUGCUGU | -10 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGGGAUGGGCGAGAUGGGGUGCUGU | -4 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGUGAUGUGCGAGAUGGUGUGCUGU | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGUGAUGUGCGAGAUGGUGUGCUGU | -1 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | GGUUGGGAGAGGGAUUUC | -10 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | GGUUGUGAGAGUGAUUUC | -1 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16308319 | McNally LM, Yee L, McNally MT. (2006) Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing. J Biol Chem. 281(5):2478-2488. | Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking, MALDI-TOF and western blot with HeLa NE | |
hnRNP H3 | GAUCACUGGGGUGGAUCAUCCAGGUGGGGCUUUU | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16396608 | Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006) Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. PLoS Biol. 4(2):e21. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Immunoprecipitation, Gel shift and RNase H protection assays | |
hnRNP H3 | GGGUCGAGGGG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16396608 | Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006) Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing. PLoS Biol. 4(2):e21. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Immunoprecipitation, Gel shift and RNase H protection assays | |
hnRNP H3 | CAGAAGGGGAGGGGUUCCA | -3 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP H3 | AACAAGGGGUGGGGGAAAA | -3 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP H3 | UGGGU | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 18806275 | Masuda A, Shen XM, Ito M, Matsuura T, Engel AG, Ohno K. (2008) hnRNP H enhances skipping of a nonfunctional exon P3A in CHRNA1 and a mutation disrupting its binding causes congenital myasthenic syndrome. Hum Mol Genet. 17(24):4022-4035. | Construct of CHRNA1 [1134] EX2-INT2-EX3-INT-EX_P3A-INT-EX4 | In vivo splicing assay in HeLa | Western blotting using HEK293T nuclear extract, immunodepletion, surface plasmon resonance | |
hnRNP H3 | GGGAAUGUGGG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 19506027 | Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009) A physical and functional link between splicing factors promotes pre-mRNA 3' end processing. Nucleic Acids Res. 37(14):4672-4683. | Sequences deriving from HBB [3043] | UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein | ||
hnRNP H3 | GAUCACUGGGGUGGAUCAU | -1 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H3 | GAUCACUGUGGUGGAUCAUCCAGGUGUGGCUUUU | -1 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H3 | GAUCACUGUGGUGGAUCAUCCAGGUGGGGCUUUU | -1 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H3 | CCAUGGUUUGGGAGUGGGAAGGUGGGGAG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H3 | CCAUGGUUUGGGGGCAGUAGUUGG | -8 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 19926721 | Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010) hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection. RNA. 16(1):228-238. | Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3 | In vitro splicing with HeLa NE | Filter binding assay, EMSA using recombinant protein | |
hnRNP H3 | GGGGA | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP H3 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 24290757 | Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V, Miller JW, Smith BN, Gallo JM, Ule J, Hirth F, Rogelj B, Houart C, Shaw CE. (2013) Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic. Cell Rep. 5(5):1178-1186. | Synthetic sequences. | RNA pull-down assay using SH-SY5Y cell extract. | ||
hnRNP H3 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -4 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP H3 | UGGGACUGGGACUGGGACUG | -5 | Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
hnRNP I (PTB) | CUCUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
hnRNP I (PTB) | UCUUC | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11788707 | Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002) Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein. Nucleic Acids Res. 30(2):456-462. | Synthesized sequences | Chemical shift mapping (NMR) | ||
hnRNP I (PTB) | CCUCUGCGCUUCUUCCCUUCCCUCCUCCCUGGCUCAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11931771 | Charlet-B N, Logan P, Singh G, Cooper TA. (2002) Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Mol Cell. 9(3):649-658. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | UV crosslink, western blot, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP I (PTB) | UUAUUUUUCCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9858533 | Lou H, Helfman DM, Gagel RF, Berget SM. (1999) Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon. Mol Cell Biol. 19(1):78-85. | Constructs of CALCA [796] EX4 - INT4 - EX5 - INT5 - EX6 fused to a heterologous first exon from adenovirus used for in vivo splicing. | In vivo splicing in HeLa and T98G cells. | EMSA, UV crosslink, immunoprecipitation with recombinant protein. | |
hnRNP I (PTB) | UUUUUUU | -7 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 8449401 | Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B. (1993) Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev. 7(3):393-406. | PSF has no affinity to poly(rA), poly(rC) and poly(rG). | Construct of tropomyosin 1 alpha TPM1 [7168] EX2 - INT2 - EX3 for splicing. Construct of branchpoint and polypyrimidine tract element upstream of TPM1 EX3 for UV-crosslink. | In vitro splicing in HeLa nuclear extracts. | RNA affinity chromatography confirmed by UV crosslink and Western blot using HeLa nuclear extracts. |
hnRNP I (PTB) | CUCCGCUCCUCUUC | 5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9858533 | Lou H, Helfman DM, Gagel RF, Berget SM. (1999) Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon. Mol Cell Biol. 19(1):78-85. | Constructs of CALCA [796] EX4 - INT4 - EX5 - INT5 - EX6 fused to a heterologous first exon from adenovirus used for in vivo splicing. | In vivo splicing in HeLa and T98G cells. | EMSA, UV crosslink, immunoprecipitation with recombinant protein. | |
hnRNP I (PTB) | UCUUCUU | -8 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9436911 | Gooding C, Roberts GC, Smith CW. (1998) Role of an inhibitory pyrimidine element and polypyrimidine tract binding protein in repression of a regulated alpha-tropomyosin exon. RNA. 4(1): 85-100. | Constructs of rat alpha-tropomyosin (TM) Tpm2 [24851] EX1 - INT - EX3 - INT3 - EX4 and EX2 - INT2 - EX3 - INT3 - EX4. | In vivo splicing in transiently transfected L fibroblast and PA smooth muscle cells | in vitro splicing in HeLa nuclear extract. | |
hnRNP I (PTB) | CCUUCCUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16260624 | Lin JC, Tarn WY. (2005) Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB. Mol Cell Biol. 25(22): 10111-10121. | PTB and RBM4 are in competition for CU1 element. PTB appear to bind with more affinity than RBM4. | Construct of alpha-TM [TPM1, 7168] EX8 - INT8 - EX9a - INT9a - EX9b and EX1 - INT1 - EX2b - INT2b - EX3. | In vivo splicing in HEK293 cells. | Mutational analysis. |
hnRNP I (PTB) | UUCUUCUUCUUCUUCUUCUUCUUCUUCUUC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
hnRNP I (PTB) | UCUUU | -6 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11788707 | Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002) Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein. Nucleic Acids Res. 30(2):456-462. | Synthesized sequences | Chemical shift mapping (NMR) | ||
hnRNP I (PTB) | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP I (PTB) | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU | -2 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP I (PTB) | CCUUUCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
hnRNP I (PTB) | UCUUUCUCCUUUUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19147685 | Bian Y, Masuda A, Matsuura T, Ito M, Okushin K, Engel AG, Ohno K. (2009) Tannic acid facilitates expression of the polypyrimidine tract binding protein and alleviates deleterious inclusion of CHRNA1 exon P3A due to an hnRNP H-disrupting mutation in congenital myasthenic syndrome. Hum Mol Genet. 18(7):1229-1237. | Construct of CHRNA1 [1134] INT3 - EX_P3A - INT_P3A. | In vivo splicing in HEK293T and HeLa cells. Downregulation by siRNA and upregulation by overexpression. | In vitro UV cross-linking and immunoprecipitation, Immunoblotting, Surface plasmon resonance analysis. | |
hnRNP I (PTB) | UCUUCUCUCUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 12649496 | Gromak N, Matlin AJ, Cooper TA, Smith CW. (2003) Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein. RNA. 9(4):443-456. | Construct of rat Actn1 [81634] EX_NM - INT | In vitro splicing with HeLa nuclear extracts. | UV cross-link with recombinant protein and with HeLa nuclear extract containing increasing protein concentrations. Mutation analysis. | |
hnRNP I (PTB) | CUUCUCUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 15840818 | Amir-Ahmady B, Boutz PL, Markovtsov V, Phillips ML, Black DL. (2005) Exon repression by polypyrimidine tract binding protein. RNA. 11(5):699-716. | Several constructs based on beta globin HBB [3043] and SRC [20779] | In vitro splicing with HeLa and WERI nuclear extracts. | UV-crosslink, EMSA | |
hnRNP I (PTB) | CUUUCUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
hnRNP I (PTB) | UUUCUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
hnRNP I (PTB) | UCUUUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
hnRNP I (PTB) | UUACUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
hnRNP I (PTB) | UCUAUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
hnRNP I (PTB) | UCCCUUUUUUUUCCACAG | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP I (PTB) | CCUUCUUCUUUUUCCUACAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP I (PTB) | CCCUUUUUUUUCCACAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP I (PTB) | CCCUUUUUUUUCCGGAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP I (PTB) | GCAGCCUGGUGCCUCCCUCUUGGCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9261396 | Tsuchihara K, Tanaka T, Hijikata M, Kuge S, Toyoda H, Nomoto A, Yamamoto N, Shimotohno K. (1997) Specific interaction of polypyrimidine tract-binding protein with the extreme 3'-terminal structure of the hepatitis C virus genome, the 3'X. J Virol. 71(9):6720-6726 | Synthesized sequences | UV cross-linking, immunoprecipitation, competition assay in PH5CH cells | ||
hnRNP I (PTB) | CCUUUUCCUUCUUCUUAUU | -8 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9292499 | Ashiya M, Grabowski PJ. (1997) A neuron-specific splicing switch mediated by an array of pre-mRNA repressor sites: evidence of a regulatory role for the polypyrimidine tract binding protein and a brain-specific PTB counterpart. RNA. 3(9):996-1015. | Constructs of rat GABRG2 [29709] EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing in HeLa nuclear extract. | UV cross-linking, competition assay, immunoprecipitation | |
hnRNP I (PTB) | UGUUUCUCUUUCUCUCCUUU | -2 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9292499 | Ashiya M, Grabowski PJ. (1997) A neuron-specific splicing switch mediated by an array of pre-mRNA repressor sites: evidence of a regulatory role for the polypyrimidine tract binding protein and a brain-specific PTB counterpart. RNA. 3(9):996-1015. | Constructs of rat GABRG2 [29709] EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing in HeLa nuclear extract. | UV cross-linking, competition assay, immunoprecipitation | |
hnRNP I (PTB) | GCAAUUCUCUUUUCUGUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9292499 | Ashiya M, Grabowski PJ. (1997) A neuron-specific splicing switch mediated by an array of pre-mRNA repressor sites: evidence of a regulatory role for the polypyrimidine tract binding protein and a brain-specific PTB counterpart. RNA. 3(9):996-1015. | Constructs of rat GABRG2 [29709] EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing in HeLa nuclear extract. | UV cross-linking, competition assay, immunoprecipitation | |
hnRNP I (PTB) | UCUUUCCUUCUUUUUUCCUUUCUUUUCCUUCCUUCUUUAAU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 10037806 | Gontarek RR, Gutshall LL, Herold KM, Tsai J, Sathe GM, Mao J, Prescott C, Del Vecchio AM. (1999) hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3'NTR of the HCV RNA genome. Nucleic Acids Res. 27(6):1457-1463. | HCV 3' NTR | UV cross-linking, immunoprecipitation, competition assay with HepG2 cell extract | ||
hnRNP I (PTB) | UUUCUCCUCUUCUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 10856256 | Conte MR, Grune T, Ghuman J, Kelly G, Ladas A, Matthews S, Curry S. (2000) Structure of tandem RNA recognition motifs from polypyrimidine tract binding protein reveals novel features of the RRM fold. EMBO J. 19(12):3132-3141. | Synthesized sequences | Filter binding assay using recombinant protein | ||
hnRNP I (PTB) | UCUCU | -6 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | UCCUCUUC | -3 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11788707 | Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002) Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein. Nucleic Acids Res. 30(2):456-462. | Synthesized sequences | Chemical shift mapping (NMR) | ||
hnRNP I (PTB) | UCUUCUCU | -6 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11788707 | Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002) Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein. Nucleic Acids Res. 30(2):456-462. | Synthesized sequences | Chemical shift mapping (NMR) | ||
hnRNP I (PTB) | CCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP I (PTB) | CAGCCACCUCUCCCCUCUCCGCACUGCUGCCA | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP I (PTB) | UACCACCACCCCUUCCCCAUUCCCUUGCCCUGCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP I (PTB) | CUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP I (PTB) | GUCUCUCUCUCUCAACCUCUUUCUUCCAAUCUCUCUUUCUCAAUCUCUCUGUUUCCCUUUGUC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 12517964 | Kosinski PA, Laughlin J, Singh K, Covey LR. (2003) A complex containing polypyrimidine tract-binding protein is involved in regulating the stability of CD40 ligand (CD154) mRNA. J Immunol. 170(2):979-988. | Sequences deriving from CD40LG [959] | UV cross-linking, EMSA with Jurkat total extracts or immunodepleted extracts | ||
hnRNP I (PTB) | UUUGUCUUCUUCUUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16109372 | Izquierdo JM, Majos N, Bonnal S, Martinez C, Castelo R, Guigo R, Bilbao D, Valcarcel J. (2005) Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition. Mol Cell. 19(4):475-484. | Sequences deriving from wt and mutated FAS [355]. Construct of FAS [355] EX5 - INT5 - EX6 - INT6 - EX7 | In vitro splicing in HeLa NE | UV cross-linking, immunoprecipitation with HeLa NE | |
hnRNP I (PTB) | CUCUCUAAAAACUCUCU | -2 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | CUCUCUAAAAAAAAAACUCUCU | -4 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | CUCUCUAAAAAAAAAAAAAAACUCUCU | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | CUCUCUAAAAAAAAAAAAAAAAAAAACUCUCU | -8 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | CUCUCUAAAAAAAAAAAAAAAAAAAAAAAAACUCUCU | -6 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | CUCUCUAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACUCUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16179478 | Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005) Structure of PTB bound to RNA: specific binding and implications for splicing regulation. Science. 309(5743):2054-2057. | Synthesized sequences | NMR, EMSA with recombinant protein | ||
hnRNP I (PTB) | UAACACCCAGUCUGUUCCCCAUGG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16950790 | Pautz A, Linker K, Hubrich T, Korhonen R, Altenhofer S, Kleinert H. (2006) The polypyrimidine tract-binding protein (PTB) is involved in the post-transcriptional regulation of human inducible nitric oxide synthase expression. J Biol Chem. 281(43):32294-32302. | Sequences deriving from NOS2 [4843] | UV cross-linking using recombinant protein | ||
hnRNP I (PTB) | GAGUUUUCUCCUCUCUCAACUUGUUCUCUCUCCUUCUUU | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16982681 | Sauliere J, Sureau A, Expert-Bezancon A, Marie J. (2006) The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit. Mol Cell Biol. 26(23):8755-8769. | Sequences deriving from chicken TPM3 [396430]. Constructs of chicken TPM3 [396430] EX5 - INT5 - EX6A - INT6 - EX6B - INT6 - EX7. | In vitro splcing in HeLa NE | EMSA with recombinant protein | |
hnRNP I (PTB) | CCUCCUUCCCUGUGCUCCUUCCGCUCCCACCUUCUUCCCUU | -1 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16982681 | Sauliere J, Sureau A, Expert-Bezancon A, Marie J. (2006) The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit. Mol Cell Biol. 26(23):8755-8769. | Sequences deriving from chicken TPM3 [396430]. Constructs of chicken TPM3 [396430] EX5 - INT5 - EX6A - INT6 - EX6B - INT6 - EX7. | In vitro splcing in HeLa NE | EMSA with recombinant protein | |
hnRNP I (PTB) | UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17466948 | Reyes R, Izquierdo JM. (2007) The RNA-binding protein PTB exerts translational control on 3'-untranslated region of the mRNA for the ATP synthase beta-subunit. Biochem Biophys Res Commun. 357(4):1107-1112. | Sequences deriving from ATP5B [506] | EMSA supershift, UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein. | ||
hnRNP I (PTB) | UUGUUUGAUUUCUUAAAGU | -8 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17507659 | Hall-Pogar T, Liang S, Hague LK, Lutz CS. (2007) Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR. RNA. 13(7):1103-1115. | Sequences deriving from PTGS2 [5743] | Western blot with HeLa NE | ||
hnRNP I (PTB) | GGCGAGAAAUAACUCAUUUCUCCUUUUUUUCUUCUCUCUUCUGUCUGAAUUCUCCCUGUCUCCCUUCCUGUGGGCCAUGGGGCUCA | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17592047 | Matlin AJ, Southby J, Gooding C, Smith CW. (2007) Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract. RNA. 13(8):1214-1223. | Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM. | In vitro splicing in HeLa NE | UV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein. | |
hnRNP I (PTB) | GGCUAUCUUUCCUGGGAACCUGCUUGGGUAUCCGCCUCCUCUCCCUCGUCACAUCUCACCUGUGGACUGGUCUUCUGCAUUUCUUUGCUCUG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17592047 | Matlin AJ, Southby J, Gooding C, Smith CW. (2007) Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract. RNA. 13(8):1214-1223. | Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM. | In vitro splicing in HeLa NE | UV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein. | |
hnRNP I (PTB) | GGGCCCUUCCUCUUUCUGCUGUCCUCCUGGCCUCUGCCUGGGCCCUCCUCCUCCCACCUGUCUGUCCCUCCUGUGUCUUGGCACCACUGCCCACAG | -7 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17592047 | Matlin AJ, Southby J, Gooding C, Smith CW. (2007) Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract. RNA. 13(8):1214-1223. | Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM. | In vitro splicing in HeLa NE | UV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein. | |
hnRNP I (PTB) | GGCGAGAAAUAACUCAUUUCUCCUUUUUUUCUUCUCUCUUCUAUCUAAAUUCUCCCUGUCUCCCUUCCUGUGGGCCAUGGGGCUCA | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17592047 | Matlin AJ, Southby J, Gooding C, Smith CW. (2007) Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract. RNA. 13(8):1214-1223. | Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM. | In vitro splicing in HeLa NE | UV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein. | |
hnRNP I (PTB) | UCUCCUUGUUUUGCUUUCGAUCUGGACUGUUCUCAGGCAAGCCGGGGAGUAACUUUUAGUUUUGCUCCUGCGAUUAUUCAACUGACGGGCUUUCAUUUCCAUUUCACAUACCCUAGCAACACUUAUACCUUGCGGAAUUGUAUUGGUAGCGUGAAAAAAGCACACUGAGAGG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 17698501 | Dhar D, Roy S, Das S. (2007) Translational control of the interferon regulatory factor 2 mRNA by IRES element. Nucleic Acids Res. 35(16):5409-5421. | Sequences deriving from IRF2 [3660] | UV cross-linking with recombinant protein | ||
hnRNP I (PTB) | CCUCUCCUUCUCUCUGCUUCUCUCUCGCUGGCCCUUAGGAGGAAGGUGGAUGUCAGGUGUGUACCGAGG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19226116 | Clerte C, Hall KB. (2009) The domains of polypyrimidine tract binding protein have distinct RNA structural preferences. Biochemistry. 48(10):2063-2074. | Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] | EMSA with recombinant protein | ||
hnRNP I (PTB) | UCCUUCUCUCUGCUUCUCUCUCGCUGGCCCUUAGGAGG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19226116 | Clerte C, Hall KB. (2009) The domains of polypyrimidine tract binding protein have distinct RNA structural preferences. Biochemistry. 48(10):2063-2074. | Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] | EMSA with recombinant protein | ||
hnRNP I (PTB) | GGAUGCUUCGCUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCUCCACCGCCCCUGUGUGUUUCCAGCUCUCUCCCCGUCCCUUUAGCUUACCCUGCAUCCCACCUGUAUGAGCCGACCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19226116 | Clerte C, Hall KB. (2009) The domains of polypyrimidine tract binding protein have distinct RNA structural preferences. Biochemistry. 48(10):2063-2074. | Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] | EMSA with recombinant protein | ||
hnRNP I (PTB) | GCAAUUCUCUUUUCUGUCUACAAAUCCAAAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19226116 | Clerte C, Hall KB. (2009) The domains of polypyrimidine tract binding protein have distinct RNA structural preferences. Biochemistry. 48(10):2063-2074. | Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] | EMSA with recombinant protein | ||
hnRNP I (PTB) | GAGACUUGGUGGCUCCAUCUUAGCCCUA | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19226116 | Clerte C, Hall KB. (2009) The domains of polypyrimidine tract binding protein have distinct RNA structural preferences. Biochemistry. 48(10):2063-2074. | Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] | EMSA with recombinant protein | ||
hnRNP I (PTB) | CACUAAGCUCGCUUUCUUGCUGUCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19506027 | Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009) A physical and functional link between splicing factors promotes pre-mRNA 3' end processing. Nucleic Acids Res. 37(14):4672-4683. | Sequences deriving from HBB [3043] | UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein | ||
hnRNP I (PTB) | UCCUCCUCCUUCCCUCUUCCUUGCCCCCUCUUCCCCUAAACCUUACAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 20010808 | David CJ, Chen M, Assanah M, Canoll P, Manley JL. (2010) HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer. Nature. 463(7279):364-368. | Sequences deriving from PKM2 [5315]. Construct of PKM2 [5315] EX9 - INT9 - AdML. Construct of PKM2 [5315] EX8 - INT8 - EX9 - INT9 - EX10 - INT10 - EX11. | In vitro splicing with HeLa NE. In vivo splicing in HeLa. | Protein affinity purification, mass spectrometry, UV cross-linking and immunoblotting with HeLa NE. siRNA. | |
hnRNP I (PTB) | UUUCUCUUUCUCUCCUUUCCUUUUCCUUCUUCUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16431980 | Clerte C, Hall KB. (2006) Characterization of multimeric complexes formed by the human PTB1 protein on RNA. RNA. 12(3):457-475. | Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR | EMSA with recombinant protein. RNA footprinting. | ||
hnRNP I (PTB) | UUCUCUUUUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16431980 | Clerte C, Hall KB. (2006) Characterization of multimeric complexes formed by the human PTB1 protein on RNA. RNA. 12(3):457-475. | Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR | EMSA with recombinant protein. RNA footprinting. | ||
hnRNP I (PTB) | UAGCUGUG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16431980 | Clerte C, Hall KB. (2006) Characterization of multimeric complexes formed by the human PTB1 protein on RNA. RNA. 12(3):457-475. | Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR | EMSA with recombinant protein. RNA footprinting. | ||
hnRNP I (PTB) | CUCCAUCUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 16431980 | Clerte C, Hall KB. (2006) Characterization of multimeric complexes formed by the human PTB1 protein on RNA. RNA. 12(3):457-475. | Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR | EMSA with recombinant protein. RNA footprinting. | ||
hnRNP I (PTB) | GGCUAACUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCUCUCUCUCUCUUUCCCGC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9305981 | Perez I, McAfee JG, Patton JG. (1997) Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein. Biochemistry. 36(39):11881-11890. | Sequences deriving from rat Tpm1 [24851] and synthesized oligos. | UV cross-linking with HeLa NE and recombinant protein. Fluorescence spectroscopy with recombinant protein. | ||
hnRNP I (PTB) | CCCCCCC | -7 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9305981 | Perez I, McAfee JG, Patton JG. (1997) Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein. Biochemistry. 36(39):11881-11890. | Sequences deriving from rat Tpm1 [24851] and synthesized oligos. | UV cross-linking with HeLa NE and recombinant protein. Fluorescence spectroscopy with recombinant protein. | ||
hnRNP I (PTB) | GGGGGGG | -2 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9305981 | Perez I, McAfee JG, Patton JG. (1997) Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein. Biochemistry. 36(39):11881-11890. | Sequences deriving from rat Tpm1 [24851] and synthesized oligos. | UV cross-linking with HeLa NE and recombinant protein. Fluorescence spectroscopy with recombinant protein. | ||
hnRNP I (PTB) | CUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP I (PTB) | CUUUCCUUUCAUUCUUUCACUUCUCU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11931771 | Charlet-B N, Logan P, Singh G, Cooper TA. (2002) Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Mol Cell. 9(3):649-658. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | UV crosslink, western blot, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP I (PTB) | UCUCUC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11931771 | Charlet-B N, Logan P, Singh G, Cooper TA. (2002) Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing. Mol Cell. 9(3):649-658. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa. | UV crosslink, western blot, immunoprecipitation in HeLa nuclear extracts. | |
hnRNP I (PTB) | UUCUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19016857 | Raponi M, Buratti E, Llorian M, Stuani C, Smith CW, Baralle D. (2008) Polypyrimidine tract binding protein regulates lternative splicing of an aberrant pseudoexon in NF1. FEBS J. 275(24): 6101-6108. | Construct of NF1 [4763] partialEX30 - INT30 - partialEX31. | In vivo splicing in Hela. | Mutagenesis, Pull down assay and Western blot with HeLa nucelar extract. | |
hnRNP I (PTB) | UUCUUC | -8 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19016857 | Raponi M, Buratti E, Llorian M, Stuani C, Smith CW, Baralle D. (2008) Polypyrimidine tract binding protein regulates lternative splicing of an aberrant pseudoexon in NF1. FEBS J. 275(24): 6101-6108. | Construct of NF1 [4763] partialEX30 - INT30 - partialEX31. | In vivo splicing in Hela. | Mutagenesis, Pull down assay and Western blot with HeLa nucelar extract. | |
hnRNP I (PTB) | GUCUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 19016857 | Raponi M, Buratti E, Llorian M, Stuani C, Smith CW, Baralle D. (2008) Polypyrimidine tract binding protein regulates lternative splicing of an aberrant pseudoexon in NF1. FEBS J. 275(24): 6101-6108. | Construct of NF1 [4763] partialEX30 - INT30 - partialEX31. | In vivo splicing in Hela. | Mutagenesis, Pull down assay and Western blot with HeLa nucelar extract. | |
hnRNP I (PTB) | CUCUUC | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | CUCUUA | -7 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | AUCUUC | -7 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | CUCUUU | -7 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | CUCUUG | -4 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | UUCUUG | -4 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | GUCUUA | -4 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 9214659 | Perez I, Lin CH, McAfee JG, Patton JG. (1997) Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo. RNA. 3(7): 764-778. | Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX. | In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells. | SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors. | |
hnRNP I (PTB) | UUCACAUUUCAUUCCCUUGUGUUUCUGUCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | CUUGACGCCUGGUGCCUCUCUCUGGCCCCCG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | UAGCAUCAGCCUGGUGCCUACCUUCGGCCCC | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | CUUGCUUCACCUGGUGCCUUCCCUUCGGCCC | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | GCUCCAGCCUGGUGGCACGCUCCUGUCCCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | AGCUGCAGCCUGGAGCUCCUCUCGUGGCCCC | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | GGCUCAGCCUGCUGACCCUUCUUCCGUCGCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | AGUGGCUGUCUGAUGCUGCUCUUCCAGCUCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | GGUAGAUGCCUGCAGCCGUUCUUCCGGUGCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | AGCCGCUGUCUGCAGACUUCCCUUCGUCGCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | GCUGUCCUGUCUUCUCCUUCUUUCCUGGUCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | ACUGUCGUCUAUUACUCUGUUCUGUUCUUCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | UUCCGUCUCACUUCUUCUUUCCGCUGUCCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | UUCCUCUCUUCCUUAUACCGUUCUGUGUGCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | UCUAGCUUUCCUUUCCUCUUCUCUCUUCCCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | UUUUUGUUGUUUUUUUU | -1 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 7761834 | Singh R, Valcarcel J, Green MR. (1995) Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins. Science. 268(5214):1173-1176. | Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. | SELEX of 31nt random and EMSA with recombinant protein. | ||
hnRNP I (PTB) | CCUCUUU | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 21518792 | Lin JC, Tarn WY. (2011) RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing. J Cell Biol. 193(3):509-520. | Constructs of wt or mutated TPM1 [7168]_EX8 - Ptbp1 [19205]_INT10 - Ptbp1 [19205]_EX11 - Ptbp1 [19205]_INT11 - TPM1 [7168]_ EX9B | In vivo splicing in C2C12 cells overexpressing human recombinant proteins | Mutagenesis, in vivo splicing assay | |
hnRNP I (PTB) | UUUAGUCAGCCUUAUAGCUAA | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
hnRNP I (PTB) | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP I (PTB) | CCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU | -1 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP I (PTB) | CCACGCACGCAGACUCGCAG | -2 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP I (PTB) | CACGCAGACAUUCAGCGGCU | -2 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP I (PTB) | GCCCAAGGAGGUGAUAGCAUUUUUCAGAGAUUGAAAAGAAUAGUAUUUGAUGCCAAAUCUACAAUUGUG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 24499931 | Han A, Stoilov P, Linares AJ, Zhou Y, Fu XD, Black DL. (2014) De novo prediction of PTBP1 binding and splicing targets reveals unexpected features of its RNA recognition and function. PLoS Comput Biol. 10(1):e1003442. | Sequences deriving from mouse Tmx3 [67988] and Scrib [105782]. | EMSA with recombinant protein | ||
hnRNP I (PTB) | GUGAGGGCUCUUUCUUCGUGGGACCGUAGAUAGGUAGCUGCUGCUGGUCUCACACUGUUCUCCCUACAG | -10 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 24499931 | Han A, Stoilov P, Linares AJ, Zhou Y, Fu XD, Black DL. (2014) De novo prediction of PTBP1 binding and splicing targets reveals unexpected features of its RNA recognition and function. PLoS Comput Biol. 10(1):e1003442. | Sequences deriving from mouse Tmx3 [67988] and Scrib [105782]. | EMSA with recombinant protein | ||
hnRNP I (PTB) | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -6 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP I (PTB) | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -4 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP I (PTB) | UCCCUUUUUUUUCCACCC | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP I (PTB) | UCCCUCCCUCCCUCCACAG | -5 | Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I. In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533). nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 2533575 | Garcia-Blanco MA, Jamison SF, Sharp PA. (1989) Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Genes Dev. 3(12A):1874-1886. | Synthesized sequences | UV cross-linking, immunoprecipitation in HeLa | ||
hnRNP J | CCCCCCC | -7 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 Isoform: isoform J is due to Alternative Splicing. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP K | CCCCCCC | -7 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP K | GGGGGGG | -4 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 7607214 | Leffers H, Dejgaard K, Celis JE. (1995) Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains. Eur J Biochem. 230(2): 447-453. | hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). | homopolymers | Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers. | |
hnRNP K | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP K | CCCCACCCUCUUCCCCAAGCCCCACCCUCUUCCCCAAG | -8 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 9160751 | Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M, Hentze MW. (1997) mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end. Cell. 16 | Constructs of luciferase gene reporter. | In vivo splicing assay with luciferase gene reporter in HeLa | UV cross-linking. Cell-free translation of different mRNAs. | |
hnRNP K | ACCCAA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCAU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCCAA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCCCAA | -2 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCCCAC | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCCCCCCCUA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCGA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCGC | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCGU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | ACCCUU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | GCCCAA | -2 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | GCCCAC | -2 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | GCCCAG | -2 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | GCCCCCCUA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | GCCCCUA | -4 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCAA | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCAC | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCAU | -2 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCCAA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCCAU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCCCAU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCCUA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCCUU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCGA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCUA | -10 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | UCCCUU | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11278705 | Thisted T, Lyakhov DL, Liebhaber SA. (2001) Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition. J Biol Chem. 276(20):17484-17496. | Sequences of 50nt random for SELEX. | SELEX of random 50nt with recombinant protein. | ||
hnRNP K | CCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP K | CAGCCACCUCUCCCCUCUCCGCACUGCUGCCA | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP K | UACCACCACCCCUUCCCCAUUCCCUUGCCCUGCU | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP K | CUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP K | CCCUCUGCCACCCAGGCAGGCCCUGCCUUCAGCCCUGGCCCAGAGCUGGAACACUCUCUGAGAUGCCCCUCUGCCUGGGCUUAUGCCCUCAGAUGGAGACAUUGGAUGUGGAGCUCCUGCUGGAUGCGUGCCCUGACCCCUGCACCAGCCCUUCCCUGCUUUGAG | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 12600897 | Skalweit A, Doller A, Huth A, Kahne T, Persson PB, Thiele BJ. (2003) Posttranscriptional control of renin synthesis: identification of proteins interacting with renin mRNA 3'-untranslated region. Circ Res. 92(4):419-427. | Sequences deriving from REN [5972] | EMSA, UV cross-linking with Calu-6 cell extracts. RNA-affinity chromatography with MALDI-TOF-MS. Western blot. | ||
hnRNP K | UCCCCCCAA | -10 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 15514164 | Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004) RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res. 95(11):1058-1066. | Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] | EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF. | ||
hnRNP K | UCCCCCAA | -10 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 15514164 | Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004) RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis. Circ Res. 95(11):1058-1066. | Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] | EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF. | ||
hnRNP K | AAAAAAA | -4 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 8917439 | Dejgaard K, Leffers H. (1996) Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains. Eur J Biochem. 241(2):425-431. | Synthesized oligos | Homopolymer binding assay with recombinant protein. | ||
hnRNP K | UUUUUUU | -2 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 8917439 | Dejgaard K, Leffers H. (1996) Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains. Eur J Biochem. 241(2):425-431. | Synthesized oligos | Homopolymer binding assay with recombinant protein. | ||
hnRNP K | AGCCACCUCCCACCCCACCCCA | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 17928403 | Lee PT, Liao PC, Chang WC, Tseng JT. (2007) Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 18(12):5004-5013. | Sequences deriving from mutated GAST [2520] and homopolymers. | EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts. | ||
hnRNP K | CCCAGCCCUGUCCCCUGAAAAACUGA | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 17928403 | Lee PT, Liao PC, Chang WC, Tseng JT. (2007) Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 18(12):5004-5013. | Sequences deriving from mutated GAST [2520] and homopolymers. | EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts. | ||
hnRNP K | CCCAGAAAUGUCCCCUGAAAAACUGA | -5 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 17928403 | Lee PT, Liao PC, Chang WC, Tseng JT. (2007) Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 18(12):5004-5013. | Sequences deriving from mutated GAST [2520] and homopolymers. | EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts. | ||
hnRNP K | AAAAGCCCUGUCAAAUGAAAAACUGA | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 17928403 | Lee PT, Liao PC, Chang WC, Tseng JT. (2007) Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover. Mol Biol Cell. 18(12):5004-5013. | Sequences deriving from mutated GAST [2520] and homopolymers. | EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts. | ||
hnRNP K | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP K | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122 hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP L | CACACCACAACGGCACCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACACACCCACACCUCGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACCCACCCACAUACAUACAU | -10 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 9880507 | Shih SC, Claffey KP. (1999) Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L. J Biol Chem. 274(3): 1359-1365. | Sequence of partial VEGFC [7424] 3'UTR | UV crosslink with M21 cell cytoplasmic extracts. Western blot, immunoprecipitation, antisense RNAs. | ||
hnRNP L | CACCCCAAACAACAACCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UGCAAAUGAAAUGACUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCUACAUCACUACAUUCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUGCAGCCCUCGUCAUUGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AGGCAAUACGCACACCACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACACACAGACAGACACACACACACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 19298794 | Lee DH, Lim MH, Youn DY, Jung SE, Ahn YS, Tsujimoto Y, Lee JH. (2009) hnRNP L binds to CA repeats in the 3'UTR of bcl-2 mRNA. Biochem Biophys Res Commun. 382(3): 583-587. | Synthesized sequences. | UV cross-linking assay and super-shift R-EMSA in MCF-7 cytoplasmic extract. | ||
hnRNP L | CACACACACACACACACACACACACACACACACACACA | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 12447348 | Hui J, Stangl K, Lane WS, Bindereif A. (2003) HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 10(1): 33-37. | In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. | Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences. | In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation. | UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis. |
hnRNP L | CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 12447348 | Hui J, Stangl K, Lane WS, Bindereif A. (2003) HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 10(1): 33-37. | In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. | Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences. | In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation. | UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis. |
hnRNP L | CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA | -8 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 12447348 | Hui J, Stangl K, Lane WS, Bindereif A. (2003) HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 10(1): 33-37. | In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. | Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences. | In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation. | UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis. |
hnRNP L | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 16001081 | Rothrock CR, House AE, Lynch KW. (2005) HnRNP L represses exon splicing via a regulated exonic splicing silencer. EMBO J. 24(15): 2792-2802. | In this context, hnRNP L does not bind to CACUGGAUUCUCACCCGGAAGUA motif. | Synthesized sequences. | RNA affinity purification, mass spectrometry, immunblotting, UV-crosslink in JSL1 nuclear extract. | |
hnRNP L | CACUGGAUUCUCACCCGGAAGUA | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP L | GACACACACGCCUGACUCAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP L | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
hnRNP L | CACUGCAGUGUCACCCGCAAGCA | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18719244 | Topp JD, Jackson J, Melton AA, Lynch KW. (2008) A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA. 14(10): 2038-2049. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vivo splicing in JSL1 cells. | UV cross-linking and immunoprecipitation in JSL1 nuclear extract. | |
hnRNP L | CACUGCUUUCUCACCCGCUAGCG | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18719244 | Topp JD, Jackson J, Melton AA, Lynch KW. (2008) A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA. 14(10): 2038-2049. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vivo splicing in JSL1 cells. | UV cross-linking and immunoprecipitation in JSL1 nuclear extract. | |
hnRNP L | CCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP L | CAGCCACCUCUCCCCUCUCCGCACUGCUGCCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP L | UACCACCACCCCUUCCCCAUUCCCUUGCCCUGCU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP L | CUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11867641 | Expert-Bezancon A, Le Caer JP, Marie J. (2002) Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA. J Biol Chem. 277(19):16614-16623. | Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7 | In vitro splicing in HeLa NE | Protein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis. | |
hnRNP L | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP L | CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
hnRNP L | CAGAAGGGGAGGGGUUCCA | -6 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP L | AACAAGGGGUGGGGGAAAA | -6 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 17567613 | Wang E, Dimova N, Cambi F. (2007) PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes. Nucleic Acids Res. 35(12):4164-4178. | Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4. | In vivo splicing in oligodendroglial precursors. | RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA | |
hnRNP L | CUGCACCACCACCUGGUUC | -3 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
hnRNP L | CUGCACCACCACCUAUCUA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
hnRNP L | UGUGUCACUGUCUGGUUC | -1 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
hnRNP L | GGCAGGAAGAAGAGGAGCA | -3 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
hnRNP L | CCAGACACCGGAAACCCCUGCCACACCAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
hnRNP L | UAUGAUAGGGACUUAGGGUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UAUGACCCGGACUCCCGGUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UAUGAUAGGCUCAUAGGGUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UAUGAUAGGCACUUAGGCUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UUAGAUCGAUGGGAAAAAAUUCGUUAAGG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UUAGAUCGAUCCCAAAAAAUUCGUUAAGG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UAAAUGUGGGGACCUAGAGGAGGAGCUGAAAAUUGUUA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UAAACUACGCGACCUAGAGGAGGAGCUGAAAAUUGUUA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | UGCAAUGUACUUGCAAACAAUGGCCUGAGUGUGCAAAGAAAAUGUCUGCUAACUGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
hnRNP L | ACAUGAACCUUCAGCGGCCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GUGCCCCAAACACCUUGCAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UCGCUGACAUACACAUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACAAUUGCACGCGACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GGAACGGCAUACUGUUGGCU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AACACACACAACGCCAUCUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GUCGGACACCAGCUCUGUGUA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CUCAUGUCCACACAUGCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UGGCCUAACACAUGAUCAGA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACACACCCACAACCCCC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UACACACACAACACAUUGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACUACACACGACCAUGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACAGCACACAACCUGGCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCACCAGCUCAUACACUACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AAACCACCCCAAACAACAAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUUACACCACACCCACUCGG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UAACACCACCCAUUGCACCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACGACAUACCAUACAGAU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UCACACGACACUACCGGCAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AAAAACACCACCACAAACAA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUGUAAUUGUUCUCCGCGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AAUCACAUACGCUUUACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CUGCACCCUCCACCACGCCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UACACACAUUCCGUGCAUGG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACUGAUGACACCACUGACAA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UCACACCACUUAAGCAAACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CGCACCACAUUACAUGCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCCGGACUACACGCAACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCGCUGCACCCACAGCCACG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CGCUGCCCUCACUCGCACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GGGGACUCUACAUCGACAUA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCAACCCCAAACCCCAACAA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UCACUAAACUCCACAGCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AACACGCACGCAUUGUUACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACGACACGACGACACAU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCCUGACACAUACACCGACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UGCAAACCUAAAUGAGGUACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UUUACAUACCAACACCAGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUACAUGACACACACACGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GUGCCAUGAACACCACGACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACUACAUAAGCACCACGA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACACACUACACCACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACAUUGCAUACAUACACGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GAUCGCGCCCGCUGCAGUCG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GUCCCUAGUAGUGUGGGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACAUUGCACACAUACCCC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AGUAACACACACCCACGGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GAACACGACAUACAUCGCAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UGCAUCUACGUACACACACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUGCACACCCACAUGUCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCCCCCACCACCACAACCCC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AAACACAACACAACCACCCC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GGUAGGACCGGUGCUGUGAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GACACACACAACACAGACGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCAAACAGUACACCAGCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACAAGCGCUCCCCGGCUCAU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GUACAUAUACACACACACGA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UUCACCUUUACCGGCAGCCU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACCGGUGUACACUACAUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CAAACACGCACCAACACACC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCACACGAAUAACAUCGCAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CUACACACGACACAUGAGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CGGCCCACACGCAUGGUCGG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AAACACCACAACACACACCC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UACACUAACAACGCACACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACGCAACCAUGCAUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCCCGCACUGACCGCACGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GACCUAUACACCGCACCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACAUACACCACACGCAUUGA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UUCACACCCCGCCGUUGACC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCAUACACACCCGCCUGGCA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACCGACGGCCUAUAUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UACGCACAUAACACAUCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AGCACCUGCACACAUACAUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACCAGCCGGCACACACACG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACAACUCACGGCCACACG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCAAACGGUGACCACUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUGAUUAACUACACGCAAGA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | UCAUGCCCUGACAGUGCACG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACUCACACUACAUAUGGC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AACUACACAUACACCGGACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACAUAAAACACAUACAUG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACAGCAUACAUACAUACACG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GACUAGCAACACACACACAG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AGACACACACCCAGCCGGCC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AAAGGGGCACAGUGUGCAG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | GCACAUAUACAUCAUAACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACGCAUACUUACAUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACAGCAUAACAUAUACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CCGGACAAACACACGAACAC | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACCACACACUGCUCACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | AUACAACACACACGACAACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | ACACCGAGACACUCACAACA | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 15889141 | Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005) Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO J. 24(11): 1988-1998. | Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6. | In vitro splicing with HeLa nuclear extracts. | Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein. | |
hnRNP L | CACACCACUGCAUUCUCACCCGCAAGCACC | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP L | UACAGCCAGCACCUUUCCUACA | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP L | UAGAGCCAGCAGCUUUCCUAGA | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP L | CACACCACA | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP L | CACCUCCAACACCACCAUCACAGCGAACACC | -2 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP L | CAGCUCCAAGACGACCAUCAGAGCGAAGACC | -1 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP L | CCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP L | CCACGCACGCAGACUCGCAG | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP L | CACGCAGACAUUCAGCGGCU | -5 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP L | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP L | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14 Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141). Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP LL | CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA | -5 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 19124611 | Rossbach O, Hung LH, Schreiner S, Grishina I, Heiner M, Hui J, Bindereif A. (2009) Auto- and cross-regulation of the hnRNP L proteins by alternative splicing. Mol Cell Biol. 29(6): 1442-1451. | In this context, hnRNP L activates inclusion of exon 6A. | Construct of hnRNPL [3191] EX1_DUP - INT1_DUP - INT6_hnRNPL - EX6A_hnRNPL - INT6_hnRNPL - INT2_DUP - EX2_DUP. Construct of hnRNPLL [92906] EX6 - INT6 - EX7. | In vitro alternative splicing assay and hnRNP L depletion/complementation and RNAi knockdown in HeLa cell in order to determine hnRNP L activity. | Pull-down assay and immunoblotting in HeLa cell nuclear extract. |
hnRNP LL | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 18719244 | Topp JD, Jackson J, Melton AA, Lynch KW. (2008) A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA. 14(10): 2038-2049. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vivo splicing in JSL1 cells. | UV cross-linking and immunoprecipitation in JSL1 nuclear extract. | |
hnRNP LL | CACUGGAUUCUCACCCGGAAGUA | -2 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 18719244 | Topp JD, Jackson J, Melton AA, Lynch KW. (2008) A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA. 14(10): 2038-2049. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vivo splicing in JSL1 cells. | UV cross-linking and immunoprecipitation in JSL1 nuclear extract. | |
hnRNP LL | CACUGCAGUGUCACCCGCAAGCA | -2 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 18719244 | Topp JD, Jackson J, Melton AA, Lynch KW. (2008) A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA. 14(10): 2038-2049. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vivo splicing in JSL1 cells. | UV cross-linking and immunoprecipitation in JSL1 nuclear extract. | |
hnRNP LL | CACACCACUGCAUUCUCACCCGCAAGCACC | -10 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | UACAGCCAGCACCUUUCCUACA | -10 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | UAGAGCCAGCAGCUUUCCUAGA | -10 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | CACACCACA | -10 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | CAGACGACA | -4 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | CACCUCCAACACCACCAUCACAGCGAACACC | -10 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | CAGCUCCAAGACGACCAUCAGAGCGAAGACC | -4 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 22402488 | Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012) HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing. Nucleic Acids Res. 40(12):5666-5678. | Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa and siRNA knockdown. | Mutational analysis, pulldown, western blot in HeLa NE | |
hnRNP LL | CCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU | -1 | Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL. hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
hnRNP M | GGGGGGG | -7 | Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP M | GAAGGAA | 5 | Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. | 24533984 | Cho S, Moon H, Loh TJ, Oh HK, Cho S, Choy HE, Song WK, Chun JS, Zheng X, Shen H. (2014) hnRNP M facilitates exon 7 inclusion of SMN2 pre-mRNA in spinal muscular atrophy by targeting an enhancer on exon 7. Biochim Biophys Acta. 1839(4):306-315. | Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vivo splicing in 293A, C33A, SH-SY5Y, GM03813 | RNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis | |
hnRNP M | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP M | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP P (TLS) | AAAAAAA | -7 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP P (TLS) | GGGGGGG | -7 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 3386636 | Swanson MS, Dreyfuss G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 8(5):2237-2241. | homopolymers | Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract. | ||
hnRNP P (TLS) | UUUUUUU | -7 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | CGGUGG | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | GGGUGA | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | GGGUGC | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | GGCGAAUUCGCUGGGGCUGGGCAGAGCGCGCAGGGUUGAGGGGAGCAGGGUCCUUCACUGGGGUGAA | -5 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | UUAGGGUUAGGGUUAGGGUUAGGG | -10 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 18776329 | Takahama K, Kino K, Arai S, Kurokawa R, Oyoshi T. (2008) Identification of RNA binding specificity for the TET-family proteins. Nucleic Acids Symp Ser (Oxf). (52):213-214. | Synthesized oligos | EMSA with recombinant protein | ||
hnRNP P (TLS) | UUGGGGUUGGGGUUGGGGUUGGGG | -1 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 18776329 | Takahama K, Kino K, Arai S, Kurokawa R, Oyoshi T. (2008) Identification of RNA binding specificity for the TET-family proteins. Nucleic Acids Symp Ser (Oxf). (52):213-214. | Synthesized oligos | EMSA with recombinant protein | ||
hnRNP P (TLS) | GGGUGG | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | GGGUGU | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | UGGUGA | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | UGGUGG | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | UGGUGU | -8 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | CGGUGA | -2 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 11098054 | Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001) Identification of an RNA binding specificity for the potential splicing factor TLS. J Biol Chem. 276(9):6807-6816. | Homopolymers. Sequences of 25 nt random for SELEX. | SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts. | ||
hnRNP P (TLS) | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP P (TLS) | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP P (TLS) | AGGGUUGAGGGGAGCAGGG | -5 | Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. | 7567462 | Sirand-Pugnet P, Durosay P, Brody E, Marie J. (1997) An intronic (A/U)GGG repeat enhances the splicing of an alternative intron of the chicken beta-tropomyosin pre-mRNA. Nucleic Acids Res. 23(17):3501-3507. | Sequences deriving from chicken TPM2 [396430] | UV crosslink and immunoprecipitation in HeLa nuclear extract | ||
hnRNP Q | AAAAAGGAAAAAAAAAAACAAAAGACAAAAAAAAAAUAAGC | -5 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 18045242 | Duning K, Buck F, Barnekow A, Kremerskothen J. (2008) SYNCRIP, a component of dendritically localized mRNPs, binds to the translation regulator BC200 RNA. J Neurochem. 105(2):351-359. | Sequences deriving from BCYRN1 [618] and homopolymers. | RNA af?nity puri?cation assays, Western blot, EMSA with RNA competitors using recombinant protein | ||
hnRNP Q | AAAAAAA | -5 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 18045242 | Duning K, Buck F, Barnekow A, Kremerskothen J. (2008) SYNCRIP, a component of dendritically localized mRNPs, binds to the translation regulator BC200 RNA. J Neurochem. 105(2):351-359. | Sequences deriving from BCYRN1 [618] and homopolymers. | RNA af?nity puri?cation assays, Western blot, EMSA with RNA competitors using recombinant protein | ||
hnRNP Q | UUUUUUU | -1 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 15340051 | Kim JH, Paek KY, Ha SH, Cho S, Choi K, Kim CS, Ryu SH, Jang SK. (2004) A cellular RNA-binding protein enhances internal ribosomal entry site-dependent translation through an interaction downstream of the hepatitis C virus polyprotein initiation codon. Mol Cell Biol. 24(18):7878-7890. | Sequences deriving from HCV2_gp1 [11027172] and homopolymers. | Protein affinity purification, MALDI-TOF, UV cross-linking and mutation analysis with HeLa S10 extracts. Competition assay using homopolymers with purified protein | ||
hnRNP Q | AUGAGCACAAAUCCUAAACCUCAAAGAAAAACC | -5 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 15340051 | Kim JH, Paek KY, Ha SH, Cho S, Choi K, Kim CS, Ryu SH, Jang SK. (2004) A cellular RNA-binding protein enhances internal ribosomal entry site-dependent translation through an interaction downstream of the hepatitis C virus polyprotein initiation codon. Mol Cell Biol. 24(18):7878-7890. | Sequences deriving from HCV2_gp1 [11027172] and homopolymers. | Protein affinity purification, MALDI-TOF, UV cross-linking and mutation analysis with HeLa S10 extracts. Competition assay using homopolymers with purified protein | ||
hnRNP Q | AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG | 5 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP Q | AUUUUCCUUACAGGGUUUCAGACAAAAUCAAAAAG | 5 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP Q | AUUUUCCUUACAGGGCUUUUUGAUUUUGUCUAAAA | 5 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
hnRNP Q | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP Q | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP U | GGGGGGG | -4 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 1628625 | Kiledjian M, Dreyfuss G. (1992) Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box. EMBO J. 11(7):2655-64. | hnRNP U has no affinity to poly(rC). | homopolymers | SDS-PAGE of ribonucleotide homopolymers with recombinant protein | |
hnRNP U | AAAAAAA | -7 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 1628625 | Kiledjian M, Dreyfuss G. (1992) Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box. EMBO J. 11(7):2655-64. | hnRNP U has no affinity to poly(rC). | homopolymers | SDS-PAGE of ribonucleotide homopolymers with recombinant protein | |
hnRNP U | UUUUUUU | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 1628625 | Kiledjian M, Dreyfuss G. (1992) Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box. EMBO J. 11(7):2655-64. | hnRNP U has no affinity to poly(rC). | homopolymers | SDS-PAGE of ribonucleotide homopolymers with recombinant protein | |
hnRNP U | ACGAAGACAAACAAA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 9765391 | Gupta AK, Drazba JA, Banerjee AK. (1998) Specific interaction of heterogeneous nuclear ribonucleoprotein particle U with the leader RNA sequence of vesicular stomatitis virus. J Virol. 72(11):8532-8540. | hnRNP U has no affinity to poly(rA). | Leader-sense (LS) RNA of Vesicular stomatitis virus (VSV). | EMSA, UV crosslink, immunoprecipitation using HeLa cell nuclear extracts. UV crosslink, competition assay with purified protein. | |
hnRNP U | CCCCCCC | -4 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 8174554 | Fackelmayer FO, Dahm K, Renz A, Ramsperger U, Richter A. (1994) Nucleic-acid-binding properties of hnRNP-U/SAF-A, a nuclear-matrix protein which binds DNA and RNA in vivo and in vitro. Eur J Biochem. 221(2):749-757. | hnRNP U has no affinity to poly(rU). | homopolymers | Filter Binding Assay with purified protein. | |
hnRNP U | UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | CUGAUUUGUAUUUAUUAGACUC | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | AACAGAAAAAGAAAUAUUU | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | CUGAUUUGUACCUAUUAGAUUC | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | UACUGAAGAACAAGUAUUU | -5 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
hnRNP U | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
hnRNP U | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
HTra2alpha | AGAAGAACGAGGAACACAA | 4 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2alpha | AAGAA | 7 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2alpha | AAGAAGAA | 8 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2alpha | AAGAAGAAGAA | 10 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2alpha | GACAGAAGAACUCUCGACAGAAGAACUCUCGACAGAAGAACU | 5 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2alpha | GAAGAGGAAG | 5 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 12403832 | Seong JY, Han J, Park S, Wuttke W, Jarry H, Kim K. (2002) Exonic splicing enhancer-dependent splicing of the gonadotropin-releasing hormone premessenger ribonucleic acid is mediated by tra2alpha, a 40-kilodalton serine/arginine-rich protein. Mol Endocrinol. 16(11):2426-2438. | Construct of mouse Gnrh1[14714] EX1 - INT1 - EX2 - EX3 - EX4 | In vitro splicing of of Gnrh1-derived construct in HeLa nuclear extract with different concentrations of the protein. | EMSA, UV-crosslink, immunoprecipitation, mutation assay | |
HTra2alpha | GAAAGUCUGAUUGAAGAGGAAGCCAGGCAGAAGAAGA | 5 | Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. | 16249178 | Park E, Han J, Son GH, Lee MS, Chung S, Park SH, Park K, Lee KH, Choi S, Seong JY, Kim K. (2006) Cooperative actions of Tra2alpha with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript. J Biol Chem. 281(1):401-409. | Sequences deriving from mouse Gnrh1 [14714]. Construct of Gnrh1 [14714] EX1 - INT1 - EX2 - EX3 - EX4. | In vitro splicing in HeLa NE | EMSA, UV cross-linking with recombinant protein | |
HTra2beta1 | AGAAGGAAGG | 3 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 10931943 | Hofmann Y, Lorson CLL, Stamm S, Androphy EJ, Wirth B. (2000) Htra2-beta 1 stimulates an exonic splicing enhancer and can restore full-length SMN expression to survival motor neuron 2 (SMN2). Proc Natl Acad Sci U S A. 97(17): 9618-9623. | Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 . Sequences and mutants of SMN1 and SMN2 EX7. | In vivo splicing in HEK293. | UV crosslink, competition assay in HeLa S100 and nuclear extracts. Immunoblotting in C33a extracts. | |
HTra2beta1 | GAAAGAAG | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 14709600 | Stoilov P, Daoud R, Nayler O, Stamm S. (2004) Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA. Hum Mol Genet. 13(5): 509-524. | Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. | Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. | Pull down assay from HEK293 nuclear extract, western blot. | |
HTra2beta1 | AGAAGAACGAGGAACACAA | 4 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2beta1 | AAGAA | 7 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2beta1 | AAGAAGAA | 8 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2beta1 | AAGAAGAAGAA | 10 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2beta1 | AAUAAGAAG | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 12649279 | Jiang Z, Tang H, Havlioglu N, Zhang X, Stamm S, Yan R, Wu JY. (2003) Mutations in tau gene exon 10 associated with FTDP-17 alter the activity of an exonic splicing enhancer to interact with Tra2 beta. J Biol Chem. 278(21):18997-19007. | Construct of Tau MAPT [4137] EX10 - INT10 - EX11. | In vitro splicing with HeLa, HEK293 and neuroblastoma N2a nuclear extracts. | UV crosslink, Western blot, immunoprecipitation, RNA interference with HeLa and HEK293 nuclear extracts. | |
HTra2beta1 | AAGAAG | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 16943417 | Wu JY, Kar A, Kuo D, Yu B, Havlioglu N. (2006) SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy. Mol Cell Biol. 26(18):6739-6747. | Construct of Tau MAPT [4137] EX9 - INT9 - EX10 - INT10 - EX11 with GFP cDNA inserted into EX11. | In vivo splicing in HEK293. | Positive clones identified by fluorescence-activated cell sorting and visual inspection. Confirmed by UV crosslink, immunoprecipitation, SDS-PAGE. | |
HTra2beta1 | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
HTra2beta1 | GACAGAAGAACUCUCGACAGAAGAACUCUCGACAGAAGAACU | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 9546399 | Tacke R, Tohyama M, Ogawa S, Manley JL. (1998) Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 93(1): 139-148. | Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. | In vitro splicing in HeLa S100 and nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein. | |
HTra2beta1 | AAGAAGAAG | 9 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 12649279 | Jiang Z, Tang H, Havlioglu N, Zhang X, Stamm S, Yan R, Wu JY. (2003) Mutations in tau gene exon 10 associated with FTDP-17 alter the activity of an exonic splicing enhancer to interact with Tra2 beta. J Biol Chem. 278(21):18997-19007. | Construct of Tau MAPT [4137] EX10 - INT10 - EX11. | In vitro splicing with HeLa, HEK293 and neuroblastoma N2a nuclear extracts. | UV crosslink, Western blot, immunoprecipitation, RNA interference with HeLa and HEK293 nuclear extracts. | |
HTra2beta1 | GAAGAAGGAAA | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 14709600 | Stoilov P, Daoud R, Nayler O, Stamm S. (2004) Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA. Hum Mol Genet. 13(5): 509-524. | Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. | Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. | Pull down assay from HEK293 nuclear extract, western blot. | |
HTra2beta1 | GAAAGAAU | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 14709600 | Stoilov P, Daoud R, Nayler O, Stamm S. (2004) Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA. Hum Mol Genet. 13(5): 509-524. | Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. | Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. | Pull down assay from HEK293 nuclear extract, western blot. | |
HTra2beta1 | GAAGAAUGAAGAAA | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 14709600 | Stoilov P, Daoud R, Nayler O, Stamm S. (2004) Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA. Hum Mol Genet. 13(5): 509-524. | Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. | Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. | Pull down assay from HEK293 nuclear extract, western blot. | |
HTra2beta1 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
HTra2beta1 | GAAGAAGAACGAAGAAGAAC | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
HTra2beta1 | GGCGACUGGGGGGUCAGGG | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 17913700 | Novoyatleva T, Heinrich B, Tang Y, Benderska N, Butchbach ME, Lorson CL, Lorson MA, Ben-Dov C, Fehlbaum P, Bracco L, Burghes AH, Bollen M, Stamm S.(2008) Protein phosphatase 1 binds to the RNA recognition motif of several splicing factors and regulates alternative pre-mRNA processing. Hum Mol Genet. 17(1):52-70. | Synthesized sequences | EMSA with recombinant protein | ||
HTra2beta1 | CAAAAAGAAGGAAGGUGCUCACAU | 10 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 19953646 | Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010) A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy. Hum Mutat. 31(1):E1110-1125. | Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein | |
HTra2beta1 | CAAAAAGAACGAAGGUGCUCACAU | 10 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 19953646 | Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010) A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy. Hum Mutat. 31(1):E1110-1125. | Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein | |
HTra2beta1 | GAAGAA | 10 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 20926394 | Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y. (2010) Structural basis for the dual RNA-recognition modes of human Tra2-ß RRM. Nucleic Acids Res. 39(4):1538-1553. | Synthesized sequences | NMR spectroscopy | ||
HTra2beta1 | GACUUCAACAAGUC | 9 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 20926394 | Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y. (2010) Structural basis for the dual RNA-recognition modes of human Tra2-ß RRM. Nucleic Acids Res. 39(4):1538-1553. | Synthesized sequences | NMR spectroscopy | ||
HTra2beta1 | UCAAC | 1 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 20926394 | Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y. (2010) Structural basis for the dual RNA-recognition modes of human Tra2-ß RRM. Nucleic Acids Res. 39(4):1538-1553. | Synthesized sequences | NMR spectroscopy | ||
HTra2beta1 | AAGAAC | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 24692659 | Moursy A, Allain FH, Clery A. (2014) Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation. Nucleic Acids Res. | Sequences deriving from SMN2 [6607]. Synthetic sequences. | NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1). | ||
HTra2beta1 | GAAGGA | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 24632473 | Cho S, Moon H, Loh TJ, Oh HK, Williams DR, Liao DJ, Zhou J, Green MR, Zheng X, Shen H. (2014) PSF contacts exon 7 of SMN2 pre-mRNA to promote exon 7 inclusion. Biochim Biophys Acta. | Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vivo splicing in 293A, C33A, SH-SY5Y along with PSF expression vector | RNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis. | |
HTra2beta1 | GAAUUA | 5 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 24632473 | Cho S, Moon H, Loh TJ, Oh HK, Williams DR, Liao DJ, Zhou J, Green MR, Zheng X, Shen H. (2014) PSF contacts exon 7 of SMN2 pre-mRNA to promote exon 7 inclusion. Biochim Biophys Acta. | Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vivo splicing in 293A, C33A, SH-SY5Y along with PSF expression vector | RNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis. | |
HTra2beta1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | 2 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
HTra2beta1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | 2 | Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
HuB | UUUUUUAUUUU | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 17035636 | Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006) A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators. Mol Biol Cell. 17(12):5105-5114. | Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus | UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts. | ||
HuB | AUUUAUUUU | -10 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAUUUA | -10 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAUUUU | -10 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAAUUU | -7 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAUUGU | -7 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | AUUUACUUU | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUAAUUUU | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUCAUUUU | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | AUUUAUACU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | AUUUAUAUA | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | CUUUAAUUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | CUUUCUUUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UAUUAUUUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UCUUAUAUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUAUACUUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUAUAUUUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUGUAUUGU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUAAUUUG | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUGAUUUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAAGUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAGUUA | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAUGUU | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAUUGA | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | UUUUAUUUG | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 7972035 | Gao FB, Carson CC, Levine T, Keene JD. (1994) Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1. Proc Natl Acad Sci U S A. 91(23):11207-11211. | Sequences of 25nt random for SELEX. | SELEX of random 25nt with purified protein. | ||
HuB | CUUUUU | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | CUUUUA | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | CUUUUUA | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | CUUUC | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | CUUUA | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | CUUUUUG | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUA | -10 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUG | -9 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUC | -3 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUUUC | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUUUA | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUUC | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | AUUUUUUUA | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | GUUUC | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | GUUUUUA | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | GUUUUC | -2 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | GUUUA | -1 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 8497264 | Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993) Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs. Mol Cell Biol. 13(6):3494-3504. | Sequences of 25 nt random for SELEX. | SELEX of 25nt random sequences with recombinant protein. | ||
HuB | UUUUUUUUUUUUU | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuB | AUUUAUUUAUUUA | -5 | Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuC | AUUUAUCUACUUUCUG | -5 | Gene Name and Synonymous: ELAVL3, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C), HUC, HUCL, PLE21, MGC20653, DKFZp547J036 | 10079173 | Sakai K, Kitagawa Y, Hirose G. (1999) Analysis of the RNA recognition motifs of human neuronal ELAV-like proteins in binding to a cytokine mRNA. Biochem Biophys Res Commun. 256(2):263-268. | Synthesized sequences | EMSA with recombinant protein | ||
HuC | UUUUUUU | -5 | Gene Name and Synonymous: ELAVL3, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C), HUC, HUCL, PLE21, MGC20653, DKFZp547J036 | 10710437 | King PH. (2000) RNA-binding analyses of HuC and HuD with the VEGF and c-myc 3'-untranslated regions using a novel ELISA-based assay. Nucleic Acids Res. 28(7):E20. | Homopolymers | ELISA and competition assays with recombinant protein | ||
HuD | UUUUUUAUUUU | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 17035636 | Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006) A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators. Mol Biol Cell. 17(12):5105-5114. | Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus | UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts. | ||
HuD | AUAUUUAUAUUUUUAUUUUAUUUUUUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 8626712 | Chung S, Jiang L, Cheng S, Furneaux H. (1996) Purification and properties of HuD, a neuronal RNA-binding protein. J Biol Chem. 271(19):11518-11524. | WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuD | AUACGUAUAUUUUUAUUUUAUUUUUUU | -8 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 8626712 | Chung S, Jiang L, Cheng S, Furneaux H. (1996) Purification and properties of HuD, a neuronal RNA-binding protein. J Biol Chem. 271(19):11518-11524. | WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuD | AUAUUUAUAUCGCUAUUUUAUUUUUUU | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 8626712 | Chung S, Jiang L, Cheng S, Furneaux H. (1996) Purification and properties of HuD, a neuronal RNA-binding protein. J Biol Chem. 271(19):11518-11524. | WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuD | AUAUUUAUAUUUUUAUGCUAUUUUUUU | -6 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 8626712 | Chung S, Jiang L, Cheng S, Furneaux H. (1996) Purification and properties of HuD, a neuronal RNA-binding protein. J Biol Chem. 271(19):11518-11524. | WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuD | UCCACUUUCCUCUCUAUUUCUCUCUG | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 9045688 | Chung S, Eckrich M, Perrone-Bizzozero N, Kohn DT, Furneaux H. (1997) The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mRNA. J Biol Chem. 272(10):6593-6598. | Sequences of human GAP43 [2596] | Filter binding assay, competition assay with recombinant protein | ||
HuD | UCUUAAUUAUUAUUUGUGUUUUAAUUUAAACACCUCCUCAUG | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 9685407 | Joseph B, Orlian M, Furneaux H. (1998) p21(waf1) mRNA contains a conserved element in its 3'-untranslated region that is bound by the Elav-like mRNA-stabilizing proteins. J Biol Chem. 273(32):20511-20516. | Sequence deriving from CDKN1A [1026] and FOS [2353] | RNase T1 selection, nitrocellulose filter binding assay, EMSA and competition assay with recombinant protein | ||
HuD | UUAUUUAUUUAUUUAUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 10848602 | Park S, Myszka DG, Yu M, Littler SJ, Laird-Offringa IA. (2000) HuD RNA recognition motifs play distinct roles in the formation of a stable complex with AU-rich RNA. Mol Cell Biol. 20(13):4765-4772. | Synthesized sequences | EMSA with recombinant protein | ||
HuD | UUAUUUAUUUAUU | -4 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 10848602 | Park S, Myszka DG, Yu M, Littler SJ, Laird-Offringa IA. (2000) HuD RNA recognition motifs play distinct roles in the formation of a stable complex with AU-rich RNA. Mol Cell Biol. 20(13):4765-4772. | Synthesized sequences | EMSA with recombinant protein | ||
HuD | UUAUUUAUUU | -3 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 10848602 | Park S, Myszka DG, Yu M, Littler SJ, Laird-Offringa IA. (2000) HuD RNA recognition motifs play distinct roles in the formation of a stable complex with AU-rich RNA. Mol Cell Biol. 20(13):4765-4772. | Synthesized sequences | EMSA with recombinant protein | ||
HuD | UUUUUUU | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12384599 | Kasashima K, Sakashita E, Saito K, Sakamoto H. (2002) Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins. Nucleic Acids Res. 30(20):4519-4526. | Homopolymers | Homopolymer binding assay with HeLa cell extracts | ||
HuD | AAAAAAA | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12384599 | Kasashima K, Sakashita E, Saito K, Sakamoto H. (2002) Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins. Nucleic Acids Res. 30(20):4519-4526. | Homopolymers | Homopolymer binding assay with HeLa cell extracts | ||
HuD | CUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUUUUUUUUU | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12605686 | Wein G, Rossler M, Klug R, Herget T. (2003) The 3'-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR. Eur J Biochem. 270(2):350-365. | Sequences deriving from mouse Marcks [17118] | EMSA, UV cross-linking with recombinant protein | ||
HuD | UUAUUUAUUUAUUUAUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UAUUUAUUUAUUUAU | -9 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UAUUUAUUUAUUUA | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | AUUUAUUUAUUUAU | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | AUUUAUUUAUUUA | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUAUUUAUUUA | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | AUUUAUUUAUUU | -6 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUAUUUAUUU | -4 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUAUUUAUUUAUUUAUUUAUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUAUUUAUUUAUUUAUUUAUUUAUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUAUUUAUUUAUUUAUUUAUUUAUUUAUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUAUUUAUUUAUUUAUUUAUUUAUUUAUUUAUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | AUUUUUUUUUUUA | -8 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUUAUUUAUUUU | -8 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUUUUUUUUUUU | -10 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | AUUUCAUUAUUUA | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UGUUUCCUUUUAU | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUAUUUACUAAU | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UGGAUUGUAUUCA | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUAUUUCCUUUAU | -7 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUGUAUCUAAAU | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UGCGUGCUUGCCU | -1 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUUAUUGCCUAA | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | CUUGUUUGCUGUU | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | CUACUGGCACGCA | -1 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | CCUACUUGUUUCU | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | GUUGUUUGUUCUA | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | GUUUUUUUCCUAC | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | GUUUUUAUAUCCA | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | AGGUUUGUGUCAG | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | CUUUUGUUCUAGC | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | CUUGAAUUUCUAC | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | UUUGCAUUUUAUC | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuD | GUCUUGACUUUUC | -5 | Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. | 12900401 | Park-Lee S, Kim S, Laird-Offringa IA. (2003) Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA. J Biol Chem. 278(41):39801-39808. | Synthesized sequences. Sequences of 13nt random for SELEX. | SELEX of random 13nt and EMSA with recombinant protein. | ||
HuR | AUUUA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9882309 | Sokolowski M, Furneaux H, Schwartz S. (1999) The inhibitory activity of the AU-rich RNA element in the human papillomavirus type 1 late 3' untranslated region correlates with its affinity for the elav-like HuR protein. J Virol. 73(2):1080-1091. | HuR has no affinity to poly(A), poly(G) and poly(C). | Sequence and mutants of human Papillomavirus Type 1 Late 3' UTR h1ARE. | UV crosslink, immunoblotting with HeLa nuclear extracts, EMSA with recombinant protein. | |
HuR | UUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9882309 | Sokolowski M, Furneaux H, Schwartz S. (1999) The inhibitory activity of the AU-rich RNA element in the human papillomavirus type 1 late 3' untranslated region correlates with its affinity for the elav-like HuR protein. J Virol. 73(2):1080-1091. | HuR has no affinity to poly(A), poly(G) and poly(C). | Sequence and mutants of human Papillomavirus Type 1 Late 3' UTR h1ARE. | UV crosslink, immunoblotting with HeLa nuclear extracts, EMSA with recombinant protein. | |
HuR | AUUUAUUUAUUUAUUUA | -8 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9155038 | Myer VE, Fan XC, Steitz JA. (1997) Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay. EMBO J. 16(8):2130-2139. | HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. | synthesized oligos | EMSA, UV crosslink, competition assay with HeLa nuclear extracts. | |
HuR | AUGUAUGUAUGUAUGUA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9155038 | Myer VE, Fan XC, Steitz JA. (1997) Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay. EMBO J. 16(8):2130-2139. | HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. | synthesized oligos | EMSA, UV crosslink, competition assay with HeLa nuclear extracts. | |
HuR | AUUUUAUUUUAUUUUA | -3 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9155038 | Myer VE, Fan XC, Steitz JA. (1997) Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay. EMBO J. 16(8):2130-2139. | HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. | synthesized oligos | EMSA, UV crosslink, competition assay with HeLa nuclear extracts. | |
HuR | UUAUUUAUUGACCUUAUUUAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9155038 | Myer VE, Fan XC, Steitz JA. (1997) Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay. EMBO J. 16(8):2130-2139. | HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. | synthesized oligos | EMSA, UV crosslink, competition assay with HeLa nuclear extracts. | |
HuR | AUAUUUAUAUUUUUAUUUUAUUUUUUU | -10 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 8626503 | Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996) Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein. J Biol Chem. 271(14):8144-8151. | WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562]. | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuR | AUACGUAUAUUUUUAUUUUAUUUUUUU | -8 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 8626503 | Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996) Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein. J Biol Chem. 271(14):8144-8151. | WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562]. | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuR | AUAUUUAUAUCGCUAUUUUAUUUUUUU | -3 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 8626503 | Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996) Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein. J Biol Chem. 271(14):8144-8151. | WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562]. | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuR | AUAUUUAUAUUUUUAUGCUAUUUUUUU | -3 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 8626503 | Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996) Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein. J Biol Chem. 271(14):8144-8151. | WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562]. | EMSA, Filter Binding Assay, RNase Protection Assay. | ||
HuR | UUUUUUU | -7 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 9882309 | Sokolowski M, Furneaux H, Schwartz S. (1999) The inhibitory activity of the AU-rich RNA element in the human papillomavirus type 1 late 3' untranslated region correlates with its affinity for the elav-like HuR protein. J Virol. 73(2):1080-1091. | HuR has no affinity to poly(A), poly(G) and poly(C). | Sequence and mutants of human Papillomavirus Type 1 Late 3' UTR h1ARE. | UV crosslink, immunoblotting with HeLa nuclear extracts, EMSA with recombinant protein. | |
HuR | UAUUAAUUUAAUUAUUUAAUAAUAUUUAUAUUAAA | -1 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | UAUUUAUUUAUUUAUUUGUUUGUUUGUUUUAUU | -10 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | UAUUUAUUUAAAUAUUUAAAUUUUAUAUUUAUU | -2 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | AUAUUUUAAUUUAUGAGUUUUUGAUAGCUUUAUUUUUUAAGUAUUUAUAUAUUUAUAA | -7 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | UAUUUAUUAUUUAUGUAUUUAUUUAA | -9 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | AUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUA | -10 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | UGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUACAGA | -9 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | UGUGAUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUACAGA | -7 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | AUUAUUUAUUAUUUAUUUAUUAAAUAUUUAUUUA | -4 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | AUUUAUUUAUUUA | -9 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | AUUUAUUUAUUUAUUUAUUUA | -10 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | CUUUCUUUCUUUCUUUC | -9 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15457527 | Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004) mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure. Chembiochem. 5(10):1432-1447. | Synthesized sequences. | 2D-FIDA anisotropy with purified protein | ||
HuR | UUUGUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
HuR | UUAUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
HuR | UUUAUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
HuR | UUGUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
HuR | UUAUUUAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 10075998 | Maurer F, Tierney M, Medcalf RL. (1999) An AU-rich sequence in the 3'-UTR of plasminogen activator inhibitor type 2 (PAI-2) mRNA promotes PAI-2 mRNA decay and provides a binding site for nuclear HuR. Nucleic Acids Res. 27(7):1664-1673. | Sequences deriving from SERPINB2 [5055] | EMSA supershift, mutation analysis with HT-1080 NE | ||
HuR | UGUUUUUUUGAGAGU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 10913178 | Millard SS, Vidal A, Markus M, Koff A. (2000) A U-rich element in the 5' untranslated region is necessary for the translation of p27 mRNA. Mol Cell Biol. 20(16):5947-5959. | Sequences deriving from CDKN1B [1027] | UV cross-linking, immunoprecipitation, mutation analysis in 293T cell | ||
HuR | UAUUUCUUGUUUGUUUGUUUGGGUAU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11602610 | Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001) HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities. J Biol Chem. 276(51):47958-47965. | Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] | UV cross-linking, immunoprecipitation in HeLa NE | ||
HuR | UUUUUUUUUUUUUUUUUUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11602610 | Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001) HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities. J Biol Chem. 276(51):47958-47965. | Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] | UV cross-linking, immunoprecipitation in HeLa NE | ||
HuR | UAUUUAUAUUUUUAUUUUAUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11602610 | Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001) HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities. J Biol Chem. 276(51):47958-47965. | Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] | UV cross-linking, immunoprecipitation in HeLa NE | ||
HuR | UAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11602610 | Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001) HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities. J Biol Chem. 276(51):47958-47965. | Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] | UV cross-linking, immunoprecipitation in HeLa NE | ||
HuR | UAUUUAUUUAUGUAUGUAUGUAUUUAUUUAUUUAU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11602610 | Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001) HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities. J Biol Chem. 276(51):47958-47965. | Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] | UV cross-linking, immunoprecipitation in HeLa NE | ||
HuR | UUACCAUCUUUUUUUUUUCUUUA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11602610 | Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001) HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities. J Biol Chem. 276(51):47958-47965. | Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] | UV cross-linking, immunoprecipitation in HeLa NE | ||
HuR | UUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 11719186 | Chen CY, Gherzi R, Ong SE, Chan EL, Raijmakers R, Pruijn GJ, Stoecklin G, Moroni C, Mann M, Karin M. (2001) AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell. 107(4):451-464. | Sequences deriving from FOS [2353] | UV crosslinking, imunoprecipitation with Jurkat extracts | ||
HuR | CUUUCUCUCCUUUCUUUUUCUUCUUC | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 12011088 | Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002) Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA. J Biol Chem. 277(30):27183-27192. | Sequences deriving from AR [367]. Synthesized oligos. | Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts | ||
HuR | CCCCCCC | -7 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 12011088 | Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002) Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA. J Biol Chem. 277(30):27183-27192. | Sequences deriving from AR [367]. Synthesized oligos. | Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts | ||
HuR | CUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUUUUUUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 12605686 | Wein G, Rossler M, Klug R, Herget T. (2003) The 3'-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR. Eur J Biochem. 270(2):350-365. | Sequences deriving from mouse Marcks [17118] | EMSA, UV cross-linking with recombinant protein | ||
HuR | UCUAUUAAUUUAAUUAUUUAAUAAUAUUUAUAUUAAACUCCUUAU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 12704185 | Sengupta S, Jang BC, Wu MT, Paik JH, Furneaux H, Hla T. (2003) The RNA-binding protein HuR regulates the expression of cyclooxygenase-2. J Biol Chem. 278(27):25227-25233. | Sequences deriving from PTGS2 [5743] | RNase T1 assay with recombinant protein | ||
HuR | CUUAAAUUCAUUUCACACAUUAAUUUUAUCUCA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 12704185 | Sengupta S, Jang BC, Wu MT, Paik JH, Furneaux H, Hla T. (2003) The RNA-binding protein HuR regulates the expression of cyclooxygenase-2. J Biol Chem. 278(27):25227-25233. | Sequences deriving from PTGS2 [5743] | RNase T1 assay with recombinant protein | ||
HuR | UGCAUGCUGUUCCUUUUCUUUUCUUCUUUUAGCCAUUUUGC | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 12704185 | Sengupta S, Jang BC, Wu MT, Paik JH, Furneaux H, Hla T. (2003) The RNA-binding protein HuR regulates the expression of cyclooxygenase-2. J Biol Chem. 278(27):25227-25233. | Sequences deriving from PTGS2 [5743] | RNase T1 assay with recombinant protein | ||
HuR | GUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAG | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 15809297 | Fialcowitz EJ, Brewer BY, Keenan BP, Wilson GM. (2005) A hairpin-like structure within an AU-rich mRNA-destabilizing element regulates trans-factor binding selectivity and mRNA decay kinetics. J Biol Chem. 280(23):22406-22417. | Synthesized sequences | EMSA, fluorescence anisotropy with recombinant protein | ||
HuR | UAUUUAUGUUUGCACUUGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUACAGAUGAAUGUAUUUAUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16168373 | Katsanou V, Papadaki O, Milatos S, Blackshear PJ, Anderson P, Kollias G, Kontoyiannis DL. (2005) HuR as a negative posttranscriptional modulator in inflammation. Mol Cell. 19(6):777-789. | Sequences deriving from wt and mutant TNF [7194] | EMSA with recombinant protein | ||
HuR | UUAUUUAUAAAUCAUUUCCUUUCUUUUUUUCCCCAAAGUCAGAAUUGCUCAAAGAAAAUUAUUUAUUGUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16289864 | Sommer S, Cui Y, Brewer G, Fuqua SA. (2005) The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 97(3):219-229. | Sequences deriving from YES1 [7525] | EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts. | ||
HuR | UUUUUUAAAGUUUCUUGCAUUUAUUAUUCUCAAAAGUUUUUUCUAAGUUAAACAGUCAGUAUGCAAUCUUAAUAUAUGCUUUCUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16289864 | Sommer S, Cui Y, Brewer G, Fuqua SA. (2005) The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 97(3):219-229. | Sequences deriving from YES1 [7525] | EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts. | ||
HuR | UUUUUAUGUAAAACAUUUUUAGAACUCCAGUUUUCAAAUCAUGUUUGAAUCUACAUUCACUUUUUUUUGUUUUCUUUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16289864 | Sommer S, Cui Y, Brewer G, Fuqua SA. (2005) The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells. J Steroid Biochem Mol Biol. 97(3):219-229. | Sequences deriving from YES1 [7525] | EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts. | ||
HuR | UGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU | -2 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
HuR | UGAACUUUAUAGUUGUUUUAUAUGUU | -2 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16787927 | Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006) Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1. J Biol Chem. 281(33):23456-23463. | Sequences deriving from wt and mutated TYMS [7298] | Pulldown assay and Western blotting in RKO whole-cell extract. | ||
HuR | UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 16890199 | Izquierdo JM. (2006) Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR. Biochem Biophys Res Commun. 348(2):703-711. | Sequences deriving from ATP5B [506] | UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein. | ||
HuR | UUUUGUUUUGGUUUUUUUUUUUUUUUUGGC | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 17548472 | Dormoy-Raclet V, Menard I, Clair E, Kurban G, Mazroui R, Di Marco S, von Roretz C, Pause A, Gallouzi IE. (2007) The RNA-binding protein HuR promotes cell migration and cell invasion by stabilizing the beta-actin mRNA in a U-rich-element-dependent manner. Mol Cell Biol. 27(15):5365-5380. | Sequences deriving from ACTB [60] | EMSA supershift with HeLa cell extracts | ||
HuR | GGGGGGUUUUUUUUUUUUUUUUUGGGGG | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 17682065 | Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA. (2007) Elucidation of a C-rich signature motif in target mRNAs of RNA-binding protein TIAR. Mol Cell Biol. 27(19):6806-6817. | Synthesized sequences | Surface plasmon resonance | ||
HuR | UUUGUCUUCUUCUUUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 18463097 | Izquierdo JM. (2008) Hu antigen R (HuR) functions as an alternative pre-mRNA splicing regulator of Fas apoptosis-promoting receptor on exon definition. J Biol Chem. 283(27):19077-19084. | Sequences deriving from FAS [355]. Constructs of FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. | In vivo splicing in HeLa. | UV cross-linking, immunoprecipitation with HeLa NE. siRNA. | |
HuR | CUUUUCUGUUUAGUUUUUACUUUUUUUGUUUUGUUUUUUUA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 18644987 | Scott GK, Marx C, Berger CE, Saunders LR, Verdin E, Schafer S, Jung M, Benz CC. (2008) Destabilization of ERBB2 transcripts by targeting 3' untranslated region messenger RNA associated HuR and histone deacetylase-6. Mol Cancer Res. 6(7):1250-1258. | Sequences deriving from ERBB2 [2064] | EMSA supershift with SKBR3 cytosol extract. | ||
HuR | CAUAAAUUAUUUUCAAGUGUAACUUAUUAACCUAUUUAUUAUUUAUGUAUUUAUUUAAGC | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 18650551 | Palanisamy V, Park NJ, Wang J, Wong DT. (2008) AUF1 and HuR proteins stabilize interleukin-8 mRNA in human saliva. J Dent Res. 87(8):772-776. | Sequences deriving from IL8 [3576] | UV cross-linking, immunoprecipitation with salivary protein extracts. | ||
HuR | GUGUUGUUUGUUGUGUAUAUGUUUGUAUGU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 18986664 | Cumming SA, Chuen-Im T, Zhang J, Graham SV. (2009) The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells. Virology. 383(1):142-149. | Sequences deriving from HPV-16 LRE. | EMSA supershift, UV cross-linking with W12 NE or recombinant protein | ||
HuR | GAUGCUGAUUCAUUAUUUAUCAGCCCUAUUCUUUC | -8 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19010922 | Saunus JM, French JD, Edwards SL, Beveridge DJ, Hatchell EC, Wagner SA, Stein SR, Davidson A, Simpson KJ, Francis GD, Leedman PJ, Brown MA. (2008) Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR. Cancer Res. 68(22):9469-9478. | Sequences deriving from BRCA1 [672] | EMSA supershift, UV cross-linking with HeLa protein extracts | ||
HuR | GAAGCUGAUUCAUUAUUUAUCAGCCCUAUUCUUUC | -8 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19010922 | Saunus JM, French JD, Edwards SL, Beveridge DJ, Hatchell EC, Wagner SA, Stein SR, Davidson A, Simpson KJ, Francis GD, Leedman PJ, Brown MA. (2008) Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR. Cancer Res. 68(22):9469-9478. | Sequences deriving from BRCA1 [672] | EMSA supershift, UV cross-linking with HeLa protein extracts | ||
HuR | GAUGCUGAGUCAUUAUUUAUCAGCCCUAUUCUUUC | -2 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19010922 | Saunus JM, French JD, Edwards SL, Beveridge DJ, Hatchell EC, Wagner SA, Stein SR, Davidson A, Simpson KJ, Francis GD, Leedman PJ, Brown MA. (2008) Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR. Cancer Res. 68(22):9469-9478. | Sequences deriving from BRCA1 [672] | EMSA supershift, UV cross-linking with HeLa protein extracts | ||
HuR | CUAGUAGAACCUUCUUUCCUAAUCCCCUUAUCUUCAUGGAAAUGGACUGACUUUAUGCCUAUGAAGUCC | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19151756 | Woo HH, Zhou Y, Yi X, David CL, Zheng W, Gilmore-Hebert M, Kluger HM, Ulukus EC, Baker T, Stoffer JB, Chambers SK. (2009) Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer. Oncogene. 28(9):1176-1186. | Sequences deriving from CSF1R [1436] | UV cross-linking with recombinant protein | ||
HuR | CUGUAUUUAUCUGUUUAUUUAUACCUAUUUAUGUAUUUAUUUAUUGAAGUGUGAAAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 19359363 | Al-Ahmadi W, Al-Ghamdi M, Al-Haj L, Al-Saif M, Khabar KS. (2009) Alternative polyadenylation variants of the RNA binding protein, HuR: abundance, role of AU-rich elements and auto-Regulation. Nucleic Acids Res. 37(11):3612-3624. | Sequences deriving from ELAVL1 [1994] | EMSA supershift with HEK293 protein lysate or recombinant protein. | ||
HuR | GCAUGCUGUUCCUUUUCUUUUCU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 20086103 | Doller A, Schlepckow K, Schwalbe H, Pfeilschifter J, Eberhardt W. (2010) Tandem phosphorylation of serines 221 and 318 by protein kinase Cdelta coordinates mRNA binding and nucleocytoplasmic shuttling of HuR. Mol Cell Biol. 30(6):1397-1410. | Sequences deriving from PTGS2 [5743] | EMSA with recombinant protein. | ||
HuR | UCAGAUAUUUAUAUUUUUAUUUUAUUUUUUUCUACCUUGAGGUCUUUUGA | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 20459669 | Ahn J, Byeon IJ, Dharmasena S, Huber K, Concel J, Gronenborn AM, Sluis-Cremer N. (2010) The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro. Retrovirology. 7:40. | Synthesized sequences | EMSA with recombinant protein | ||
HuR | CCAUUUAUAUCAUUUUUUAUAUAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
HuR | CCAUUUAUAUCAUAAAAUAUAUAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
HuR | CCAUUUAUAUCAUUUUUAAAAAAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
HuR | CCAUUUAUAUCAUAAAAAUAUUAUU | -5 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 20498276 | Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010) HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay. Mol Cell Biol. 30(15):3875-3886. | Sequences deriving from CDKN2A [1029] | Protein affinity purification, western blotting with HeLa cytoplasmic extracts | ||
HuR | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
HuR | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1 HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
KSRP | UGCAUG | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
KSRP | UUAGGGUCACACCCACCACUGGGAGAU | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 19458619 | Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009) The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs. Nature. 459(7249):1010-1014. | MIRLET7A1 [406881], MIR21 [406991] and mutants. | EMSA with purified protein | ||
KSRP | UUAGGGUCACACCCACCACUCCCAGAU | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 19458619 | Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009) The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs. Nature. 459(7249):1010-1014. | MIRLET7A1 [406881], MIR21 [406991] and mutants. | EMSA with purified protein | ||
KSRP | CUGUUGAAUCUCAUGG | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 19458619 | Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009) The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs. Nature. 459(7249):1010-1014. | MIRLET7A1 [406881], MIR21 [406991] and mutants. | EMSA with purified protein | ||
KSRP | CUGUUGAAUCUCAUCC | -2 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 19458619 | Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009) The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs. Nature. 459(7249):1010-1014. | MIRLET7A1 [406881], MIR21 [406991] and mutants. | EMSA with purified protein | ||
KSRP | UUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 11719186 | Chen CY, Gherzi R, Ong SE, Chan EL, Raijmakers R, Pruijn GJ, Stoecklin G, Moroni C, Mann M, Karin M. (2001) AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell. 107(4):451-464. | Sequences deriving from FOS [2353] | UV crosslinking, imunoprecipitation with Jurkat extracts | ||
KSRP | AUUUUA | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 16126846 | Linker K, Pautz A, Fechir M, Hubrich T, Greeve J, Kleinert H. (2005) Involvement of KSRP in the post-transcriptional regulation of human iNOS expression-complex interplay of KSRP with TTP and HuR. Nucleic Acids Res. 33(15):4813-4827. | Sequences deriving from NOS2 [4843] | UV cross-linking with recombinant protein | ||
KSRP | CAUAAAUUAUUUUCAAGUGUAACUUAUUAACCUAUUUAUUAUUUAUGUAUUUAUUUAAGC | -10 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17908789 | Winzen R, Thakur BK, Dittrich-Breiholz O, Shah M, Redich N, Dhamija S, Kracht M, Holtmann H. (2007) Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets. Mol Cell Biol. 27(23):8388-8400. | Sequences deriving from IL8 [3576] | EMSA with recombinant protein | ||
KSRP | CUAUUUAUUAUUUAUGUAUGUAUUUAAGC | -9 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17908789 | Winzen R, Thakur BK, Dittrich-Breiholz O, Shah M, Redich N, Dhamija S, Kracht M, Holtmann H. (2007) Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets. Mol Cell Biol. 27(23):8388-8400. | Sequences deriving from IL8 [3576] | EMSA with recombinant protein | ||
KSRP | CUAUUUAUUAUUUAUGUAUUUAUUUAAGC | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17908789 | Winzen R, Thakur BK, Dittrich-Breiholz O, Shah M, Redich N, Dhamija S, Kracht M, Holtmann H. (2007) Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets. Mol Cell Biol. 27(23):8388-8400. | Sequences deriving from IL8 [3576] | EMSA with recombinant protein | ||
KSRP | UAUUUAUUAUUUAUUUAUUAUUUAU | -10 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17437720 | Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007) The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation. Structure. 15(4):485-498. | Sequences deriving from TNF [7124] | NMR and CD with recombinant protein | ||
KSRP | UAUUUAUUAUUU | -10 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17437720 | Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007) The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation. Structure. 15(4):485-498. | Sequences deriving from TNF [7124] | NMR and CD with recombinant protein | ||
KSRP | UAUUUA | -2 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17437720 | Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007) The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation. Structure. 15(4):485-498. | Sequences deriving from TNF [7124] | NMR and CD with recombinant protein | ||
KSRP | UAUUAU | -2 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 17437720 | Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007) The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation. Structure. 15(4):485-498. | Sequences deriving from TNF [7124] | NMR and CD with recombinant protein | ||
KSRP | GUCUCUUCCAAUGAUUCCAUUUCAAUAUAUUCUUCUUUUUAAAGUAUUACACAUUUCCACUU | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18583400 | Nechama M, Ben-Dov IZ, Briata P, Gherzi R, Naveh-Many T. (2008) The mRNA decay promoting factor K-homology splicing regulator protein post-transcriptionally determines parathyroid hormone mRNA levels. FASEB J. 22(10):3458-3468. | Sequences deriving from rat Pth [24694] | UV cross-linking, competition assay with HEK293 total extracts. | ||
KSRP | UUGGG | -2 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18684992 | Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008) The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Res. 36(16):5290-5296. | Synthesized oligos | NMR, scaffold-independent analysis (SIA) with recombinant protein | ||
KSRP | UUUAG | -1 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18684992 | Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008) The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Res. 36(16):5290-5296. | Synthesized oligos | NMR, scaffold-independent analysis (SIA) with recombinant protein | ||
KSRP | UGGGU | -10 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18684992 | Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008) The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Res. 36(16):5290-5296. | Synthesized oligos | NMR, scaffold-independent analysis (SIA) with recombinant protein | ||
KSRP | UAGGG | -5 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18684992 | Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008) The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Res. 36(16):5290-5296. | Synthesized oligos | NMR, scaffold-independent analysis (SIA) with recombinant protein | ||
KSRP | UAGUAU | -6 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18684992 | Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008) The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Res. 36(16):5290-5296. | Synthesized oligos | NMR, scaffold-independent analysis (SIA) with recombinant protein | ||
KSRP | UAGGUA | -7 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 18684992 | Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008) The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Res. 36(16):5290-5296. | Synthesized oligos | NMR, scaffold-independent analysis (SIA) with recombinant protein | ||
KSRP | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -6 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
KSRP | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -4 | Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
MBNL1 | UGUCUCGCU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 15257297 | Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004) Muscleblind proteins regulate alternative splicing. EMBO J. 23(15): 3103-3112. | MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. | WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. | Mutational analysis, UV-crosslinking assay using purified recombinant protein. | |
MBNL1 | CGCUGCGGC | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 15257297 | Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004) Muscleblind proteins regulate alternative splicing. EMBO J. 23(15): 3103-3112. | MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. | WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. | Mutational analysis, UV-crosslinking assay using purified recombinant protein. | |
MBNL1 | CGCUUU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 15257297 | Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004) Muscleblind proteins regulate alternative splicing. EMBO J. 23(15): 3103-3112. | MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. | WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. | Mutational analysis, UV-crosslinking assay using purified recombinant protein. | |
MBNL1 | UGCUGCUUUU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 15257297 | Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004) Muscleblind proteins regulate alternative splicing. EMBO J. 23(15): 3103-3112. | MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. | WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. | Mutational analysis, UV-crosslinking assay using purified recombinant protein. | |
MBNL1 | CUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUG | -6 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUG | -8 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCG | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAG | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAG | -6 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUG | -6 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCG | -8 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CAUGCAUGCAUGCAUGCAUGCAUGCAUG | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CCUGCCUGCCUGUCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CCUGCCUGCCUGCCUGCCUGGCUGCCUGUCUGCCUGCCUGCCUGCCUG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CUGCUGUUCGCUGCUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CUGCUGCUGUUCGCUGCUGCUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CUGCUGCUGCUGUUCGCUGCUGCUGCUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CCUGCCUGUUCGCCUGCCUG | -8 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CCUGCCUGCCUGUUCGCCUGCCUGCCUG | -9 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CUGCCUGCCUGUUCGCUGCCUGCCUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CCUGCCUGCCUGCCUGUUCGCCUGCCUGCCUGCCUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CCGCCGUUCGCCGCCG | -9 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CUGCUGUUCGCCGCCG | -9 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CCGCCGUUCGCAGCAG | -7 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CAGCAGUUCGCAGCAG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CAGCAGUUCGCGGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CGGCGGUUCGCGGCGG | -3 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CGGCGGUUCGCUGCUG | -2 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | GUCUCGCGUUCGCGCUGCGGC | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | GUCUCGCUGUUCGCGCUGCGGC | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CUGUCUCGUUCGCGCUGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | UGUCUCGCUUUUCCCCUCCGCUGCGGC | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | UGUCUGCUGUUUUCCCCUCCCUGCUGGC | -2 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | UUUCUCCCUUUUCCCCUCCCCUUCGGC | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | UGUCUCUCUUUUCCCCUCCGCGGCGGC | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | GCGCUUGUGC | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17702765 | Yuan Y, Compton SA, Sobczak K, Stenberg MG, Thornton CA, Griffith JD, Swanson MS. (2007) Muscleblind-like 1 interacts with RNA hairpins in splicing target and pathogenic RNAs. Nucleic Acids Res. 35(16): 5474-5486. | WT and mutated RNA substrates of TNNT3 [7140] INT8. | UV-crosslinking, mutational analysis, using HEK293T lysate overexpressing recombinant protein. | ||
MBNL1 | CCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAG | -2 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 14722159 | Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004) Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats. Hum. Mol. Genet. 13:495–507. | Synthesized sequences. | Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes. | ||
MBNL1 | CUCCUCUUCGGUGGUG | -2 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 17942744 | Warf MB, Berglund JA. (2007) MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T. RNA. 13(12): 2238-2251. | Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5. | In vivo splicing in HeLa cells. | EMSA using recombinant protein. | |
MBNL1 | CUGCUU | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UCGCUU | -8 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCUU | -7 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCUG | -4 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCUA | -3 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | ACGCUU | -3 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCGCUG | -3 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCCU | -3 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCGCUU | -3 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | AUGCUU | -2 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCUUCUGCUUCUGCUUCUGCUUCUGCUUCUGCUU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCCUCUGCCUCUGCCUCUGCCUCUGCCUCUGCCU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCGCUUCCGCUUCCGCUUCCGCUUCCGCUUCCGCUU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCUCUGCUUGCUUGCUUGCUGUUUAUGUUAAUGCGCUCGCUUGAACCCCACUGGCCC | -9 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCUUUAUUGUGCAUGCUUGCUUAGUCUUGUUAUUCGUUGUAUAU | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UGCCACUGCUGCUGCUUGCUGCUGCUGCGCUCGCUUCCAGUCAGGGUGGGCCGC | -8 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GCACCUCUGCUUGCUUGCUUGCUGUUUACCUGUAUGUUAAUUCGCUCGCUUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GCCGAGGAGGUGGUGUGAUUGCUUGCUUUAGCGCCGUCAUUUUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GCGGCGGGCAGCUGUGCUUGCUGGAGAGCAGAUGCUUGCUUCACCAAU | -2 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GGCUUU | 5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20519504 | Sen S, Talukdar I, Liu Y, Tam J, Reddy S, Webster NJ. (2010) Muscleblind-like 1 (Mbnl1) promotes insulin receptor exon 11 inclusion via binding to a downstream evolutionarily conserved intronic enhancer. J Biol Chem. 285(33):25426-25437. | Sequences deriving from INSR [3643] and rat Insr [24954]. Constructs of INSR [3643] EX10 - INT10 - EX11 - INT11 - EX12. | In vivo splicing in HeLa, HepG2 and HEK293 | RNA-affinity purification, western blot with HeLa NE | |
MBNL1 | GGGAUUUAUGGGGCUUUUUA | 5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20519504 | Sen S, Talukdar I, Liu Y, Tam J, Reddy S, Webster NJ. (2010) Muscleblind-like 1 (Mbnl1) promotes insulin receptor exon 11 inclusion via binding to a downstream evolutionarily conserved intronic enhancer. J Biol Chem. 285(33):25426-25437. | Sequences deriving from INSR [3643] and rat Insr [24954]. Constructs of INSR [3643] EX10 - INT10 - EX11 - INT11 - EX12. | In vivo splicing in HeLa, HepG2 and HEK293 | RNA-affinity purification, western blot with HeLa NE | |
MBNL1 | UUGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCUG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCGCUA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UCGCUG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCUA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CCGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UCGCUA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | AUGCCG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCCG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCCU | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCCA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCCC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | AUGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GUGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | ACGCUA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | ACGCUG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GCGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GCGCUG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GCGCUU | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCCC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UUGCCG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GUGCUA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GUGCUG | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | GUGCUU | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | ACGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | UCGCUC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | AUGCCA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | AUGCCC | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CUGCCA | -1 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20071745 | Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010) MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing. Nucleic Acids Res. 38(7):2467-2484. | Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22. | In vivo splicing in HeLa | SELEX of 32nt random and EMSA with recombinant protein. | |
MBNL1 | CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG | 5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 20186122 | Sellier C, Rau F, Liu Y, Tassone F, Hukema RK, Gattoni R, Schneider A, Richard S, Willemsen R, Elliott DJ, Hagerman PJ, Charlet-Berguerand N. (2010) Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. EMBO J. 29(7):1248-1261. | Sequences deriving from FMR1 [2332]. | FISH/IF and immunohistochemistry in human brain (hippocampal sections) of FXTAS patients. | ||
MBNL1 | CCCGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 24377303 | Yadav AR, Mace CR, Miller BL. (2014) Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method. Anal Chem. 86(2):1067-1075. | Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. | Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein. | ||
MBNL1 | CCCGCUGCUGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 24377303 | Yadav AR, Mace CR, Miller BL. (2014) Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method. Anal Chem. 86(2):1067-1075. | Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. | Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein. | ||
MBNL1 | CCCGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 24377303 | Yadav AR, Mace CR, Miller BL. (2014) Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method. Anal Chem. 86(2):1067-1075. | Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. | Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein. | ||
MBNL1 | CCCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 24377303 | Yadav AR, Mace CR, Miller BL. (2014) Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method. Anal Chem. 86(2):1067-1075. | Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. | Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein. | ||
MBNL1 | CCCGCUGCUGCUGCUGCUGCAGCAGCAGCAGCAGCGG | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 24377303 | Yadav AR, Mace CR, Miller BL. (2014) Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method. Anal Chem. 86(2):1067-1075. | Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. | Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein. | ||
MBNL1 | AUAUAUAUAUGUGCGCUUGUGCCCACAUAUAUAUA | -5 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 24377303 | Yadav AR, Mace CR, Miller BL. (2014) Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method. Anal Chem. 86(2):1067-1075. | Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. | Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein. | ||
MBNL1 | CUGCUGCUGCUGCUGCUG | -10 | Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174. MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297). MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159). | 23423380 | Reddy K, Zamiri B, Stanley SY, Macgregor RB Jr, Pearson CE. (2013) The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures. J Biol Chem. 288(14):9860-9866. | Synthetic sequences. | EMSA with recombinant protein. | ||
Nova-1 | AUCACC | 10 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | ACCACC | 6 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | UCAUAAGUCAUAAACAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUCACAUUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUCAUUCAUUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUCGCAUUUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUUAACAUUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUUCAUCGUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUUCAUUCAU | 7 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUUUCAUCUACAUAUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUUUCAUCUCAU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUUUCAUUUCAC | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCUGGCCAUGCAUCA | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | GGGGGGG | 7 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 8558240 | Buckanovich RJ, Yang YY, Darnell RB. (1996) The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies. J Neurosci. 16(3):1114-1122. | Nova-1 has no affinity to poly(A). | Homopolymers | Immunoblots and Filter Binding Assay. | |
Nova-1 | CCCCCCC | 3 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 8558240 | Buckanovich RJ, Yang YY, Darnell RB. (1996) The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies. J Neurosci. 16(3):1114-1122. | Nova-1 has no affinity to poly(A). | Homopolymers | Immunoblots and Filter Binding Assay. | |
Nova-1 | UUUUUUU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 8558240 | Buckanovich RJ, Yang YY, Darnell RB. (1996) The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies. J Neurosci. 16(3):1114-1122. | Nova-1 has no affinity to poly(A). | Homopolymers | Immunoblots and Filter Binding Assay. | |
Nova-1 | UCAUUUUCAUUUUCAUUU | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UCAUAAUCAUAAUCAUAA | 5 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 9154818 | Buckanovich RJ, Darnell RB. (1997) The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo. Mol Cell Biol. 17(6):3194-3201. | Sequences of 52 nt random for SELEX. | SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein. | ||
Nova-1 | UUCAU | 8 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 17655233 | Beuth B, Garcia-Mayoral MF, Taylor IA, Ramos A. (2007) Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex. J Am Chem Soc. 129(33):10205-10210. | Synthesized oligos | NMR titrations using recombinant protein | ||
Nova-1 | UUCAG | 8 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 17655233 | Beuth B, Garcia-Mayoral MF, Taylor IA, Ramos A. (2007) Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex. J Am Chem Soc. 129(33):10205-10210. | Synthesized oligos | NMR titrations using recombinant protein | ||
Nova-1 | UGUGU | 2 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 17655233 | Beuth B, Garcia-Mayoral MF, Taylor IA, Ramos A. (2007) Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex. J Am Chem Soc. 129(33):10205-10210. | Synthesized oligos | NMR titrations using recombinant protein | ||
Nova-1 | AUCAUC | 6 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | AGCACC | 3 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | AACACC | 3 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | AUCAAC | 3 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | UUCAAC | 6 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-1 | CUCAAC | 6 | Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | AUCACC | 10 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | ACCACC | 6 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | GAGACAU | 1 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 9789075 | Yang YY, Yin GL, Darnell RB. (1998) The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia. Proc Natl Acad Sci U S A. 95(22):13254-13259. | Nova-2 has no affinity to poly(A) and poly(C). | Homopolymers for immunoblot. Sequences 52-nt random for SELEX. | SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers. | |
Nova-2 | GAGUCAU | 10 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 9789075 | Yang YY, Yin GL, Darnell RB. (1998) The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia. Proc Natl Acad Sci U S A. 95(22):13254-13259. | Nova-2 has no affinity to poly(A) and poly(C). | Homopolymers for immunoblot. Sequences 52-nt random for SELEX. | SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers. | |
Nova-2 | GAGGCAU | 1 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 9789075 | Yang YY, Yin GL, Darnell RB. (1998) The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia. Proc Natl Acad Sci U S A. 95(22):13254-13259. | Nova-2 has no affinity to poly(A) and poly(C). | Homopolymers for immunoblot. Sequences 52-nt random for SELEX. | SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers. | |
Nova-2 | UUUUUUU | 7 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 9789075 | Yang YY, Yin GL, Darnell RB. (1998) The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia. Proc Natl Acad Sci U S A. 95(22):13254-13259. | Nova-2 has no affinity to poly(A) and poly(C). | Homopolymers for immunoblot. Sequences 52-nt random for SELEX. | SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers. | |
Nova-2 | GGGGGGG | 7 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 9789075 | Yang YY, Yin GL, Darnell RB. (1998) The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia. Proc Natl Acad Sci U S A. 95(22):13254-13259. | Nova-2 has no affinity to poly(A) and poly(C). | Homopolymers for immunoblot. Sequences 52-nt random for SELEX. | SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers. | |
Nova-2 | AUCAC | 5 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10676814 | Lewis HA, Musunuru K, Jensen KB, Edo C, Chen H, Darnell RB, Burley SK. (2000) Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome. Cell. 100(3):323-332. | Synthesized sequences | X-ray crystallography with recombinant protein. | ||
Nova-2 | AUCAUC | 6 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | AGCACC | 3 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | AACACC | 3 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | AUCAAC | 3 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | UUCAAC | 6 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
Nova-2 | CUCAAC | 6 | Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. | 10811881 | Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000) The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain. Proc Natl Acad Sci U S A. 97(11): 5740-5745. | Sequences of 25-nt random. | SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein. | ||
nPTB | CUCUCU | -5 | Gene Name and Synonymous: PTBP2, polypyrimidine tract binding protein 2, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897. nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11003644 | Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000) Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein. Mol Cell Biol. 20(20):7463-7479. | Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing. | In vitro splicing with WERI-1 extracts. | RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot. | |
nPTB | CUGAGGCUGCCCGCUGCUCUCUGCAUGUGCUUCCU | -5 | Gene Name and Synonymous: PTBP2, polypyrimidine tract binding protein 2, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897. nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 11571276 | Caputi M, Zahler AM. (2001) Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family. J Biol Chem. 276(47): 43850-43859. | Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. | RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography. | ||
nPTB | UUUAGUCAGCCUUAUAGCUAA | -5 | Gene Name and Synonymous: PTBP2, polypyrimidine tract binding protein 2, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897. nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092). | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
PSF | UUUUUUU | -7 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 8449401 | Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B. (1993) Cloning and characterization of PSF, a novel pre-mRNA splicing factor. Genes Dev. 7(3):393-406. | PSF has no affinity to poly(rA), poly(rC) and poly(rG). | Construct of tropomyosin 1 alpha TPM1 [7168] EX2 - INT2 - EX3 for splicing. Construct of branchpoint and polypyrimidine tract element upstream of TPM1 EX3 for UV-crosslink. | In vitro splicing in HeLa nuclear extracts. | RNA affinity chromatography confirmed by UV crosslink and Western blot using HeLa nuclear extracts. |
PSF | CACUGCAUUCUCACCCGCAAGCA | -5 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
PSF | CACUGGAUUCUCACCCGGAAGUA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17664280 | Melton AA, Jackson J, Wang J, Lynch KW. (2007) Combinatorial control of signal-induced exon repression by hnRNP L and PSF. Mol Cell Biol. 27(19): 6972-6984. | In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. | Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7. | In vitro splicing in JSL1 nuclear extract. | Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract. |
PSF | AAGGACC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | AGAGGAA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | AGAGGUA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GAAGAGGAA | -6 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GAGAGGA | -10 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GGACUGGGA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GGAGGAAC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GGAGGG | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GGGGGGGAUC | -6 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GGUAAGAGC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UAAGGGAUC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UAGAGAGG | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UAGAUCGGAA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UAGGGGGAC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UCUAAGGAA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UGCAGGCA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UGGAAGAAC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UGGAGGAC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UGGCAGGGGGAUC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UGGUCUGGAGC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UUGAAGCAA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | UUGUUUGAUUUCUUAAAGU | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17507659 | Hall-Pogar T, Liang S, Hague LK, Lutz CS. (2007) Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR. RNA. 13(7):1103-1115. | Sequences deriving from PTGS2 [5743] | Western blot with HeLa NE | ||
PSF | UUAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUU | -4 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
PSF | UUUUUAAAUAUUUAUUUAUUUAUUUAUUUAUUUU | -4 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
PSF | GUUUUUAAUUUAUUUAUUAAGAUGGAUUCU | -4 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
PSF | AAACCUAUUUAUUAAUAUUUAAAACUAUUUAUAUG | -4 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
PSF | CUAAUGAUCAUAUUUAUUUAUUUAUAUGAACCAUG | -4 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
PSF | CCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU | -5 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
PSF | GAAGGA | 5 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 24632473 | Cho S, Moon H, Loh TJ, Oh HK, Williams DR, Liao DJ, Zhou J, Green MR, Zheng X, Shen H. (2014) PSF contacts exon 7 of SMN2 pre-mRNA to promote exon 7 inclusion. Biochim Biophys Acta. | Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vivo splicing in 293A, C33A, SH-SY5Y along with PSF expression vector | RNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis. | |
PSF | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -10 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
PSF | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -1 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
PSF | GAGGGAAC | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
PSF | GAGAGGAA | -2 | Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. | 12403470 | Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002) PSF and p54nrb bind a conserved stem in U5 snRNA. RNA. 8(10):1334-1347. | Sequences of 20nt random for SELEX. | SELEX of random 20nt with recombinant protein. | ||
QKI | CGGGGGCCUCAGUGUGCCAGCCUCCGGGCCCUAGCUGGGCUUCGGGGUUGGUG | -5 | Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923. QKI induces exon skipping [11917126] | 11917126 | Wu JI, Reed RB, Grabowski PJ, Artzt K. (2002) Function of quaking in myelination: regulation of alternative splicing. Proc Natl Acad Sci U S A. 99(7):4233-4238. | Sequences deriving from mouse Mag [17136]. | EMSA supershift, competition assay, UV cross-linking with HeLa NE. | ||
QKI | AUUAAC | -10 | Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923. QKI induces exon skipping [11917126] | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
QKI | CUAAU | -10 | Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923. QKI induces exon skipping [11917126] | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
QKI | CGAGU | -3 | Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923. QKI induces exon skipping [11917126] | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
QKI | ACUUAU | -1 | Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923. QKI induces exon skipping [11917126] | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
QKI | ACUAAU | -10 | Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923. QKI induces exon skipping [11917126] | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
RBM25 | CGGGCA | -5 | Gene Name and Synonymous: RBM25, RNA binding motif protein 25, S164, RNPC7, RED120, fSAP94, MGC105088, MGC117168. RBM25 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 (PMID: 18663000). | 18663000 | Zhou A, Ou AC, Cho A, Benz EJ Jr, Huang SC. (2008) Novel splicing factor RBM25 modulates Bcl-x pre-mRNA 5' splice site selection. Mol Cell Biol. 28(19): 5924-5936. | Construct of Bcl-X [BCL2L1, 598] EX1 - INT1 - EX2 - INT2 - EX3 with wt or mutated EX2. Construct and variants of Adenovirus E1A unit. | In vivo splicing assay in HeLa cell | Mutational analysis, immuoprecipitation assay in HeLa cells, EMSA and competition assay with recombinant protein. | |
RBM25 | AUCGGGCA | -5 | Gene Name and Synonymous: RBM25, RNA binding motif protein 25, S164, RNPC7, RED120, fSAP94, MGC105088, MGC117168. RBM25 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 (PMID: 18663000). | 23190262 | Gong D, Yang F, Li F, Qian D, Wu M, Shao Z, Wu M, Wu J, Shi Y. (2013) Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region. Biochem J. 450(1):85-94. | Synthetic sequence | Fluorescence polarization assay | ||
RBM4 | CCUUCCUU | 4 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 16260624 | Lin JC, Tarn WY. (2005) Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB. Mol Cell Biol. 25(22): 10111-10121. | PTB and RBM4 are in competition for CU1 element. PTB appear to bind with more affinity than RBM4. | Construct of alpha-TM [TPM1, 7168] EX8 - INT8 - EX9a - INT9a - EX9b and EX1 - INT1 - EX2b - INT2b - EX3. | In vivo splicing in HEK293 cells. | Mutational analysis. |
RBM4 | UCCUUCUUG | 5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 16777844 | Kar A, Havlioglu N, Tarn WY, Wu JY. (2006) RBM4 interacts with an intronic element and stimulates tau exon 10 inclusion. J Biol Chem. 281(34): 24479-24488. | WT and mutant constucts of Tau MAPT [4137] EX9 - INT9 - EX10 - INT10 - EX11. | In vivo splicing in HEK293 cells. | Mutational analysis, UV cross-linking assay with purified recombinant protein. | |
RBM4 | GCGCGCG | 5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
RBM4 | GCGCGGG | 5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
RBM4 | CGCGCGG | 5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
RBM4 | CGCGCGA | 5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
RBM4 | GCGCGGU | 5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
RBM4 | CCUCUUU | -5 | Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918. RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844). | 21518792 | Lin JC, Tarn WY. (2011) RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing. J Cell Biol. 193(3):509-520. | Constructs of wt or mutated TPM1 [7168]_EX8 - Ptbp1 [19205]_INT10 - Ptbp1 [19205]_EX11 - Ptbp1 [19205]_INT11 - TPM1 [7168]_ EX9B | In vivo splicing in C2C12 cells overexpressing human recombinant proteins | Mutagenesis, in vivo splicing assay | |
RBM5 | GGGGGGG | -7 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 11029660 | Edamatsu H, Kaziro Y, Itoh H. (2000) LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells. Genes Cells. 5(10):849-858. | Homopolymers | Homopolymer binding assay with recombinant protein. | ||
RBM5 | CCCCCCC | -5 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 11029660 | Edamatsu H, Kaziro Y, Itoh H. (2000) LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells. Genes Cells. 5(10):849-858. | Homopolymers | Homopolymer binding assay with recombinant protein. | ||
RBM5 | AAAAAAA | -1 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 11029660 | Edamatsu H, Kaziro Y, Itoh H. (2000) LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells. Genes Cells. 5(10):849-858. | Homopolymers | Homopolymer binding assay with recombinant protein. | ||
RBM5 | CUCUUCUCU | -5 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 18840686 | Fushimi K, Ray P, Kar A, Wang L, Sutherland LC, Wu JY. (2008) Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5. Proc Natl Acad Sci U S A. 105(41):15708-15713. | Sequences deriving from mouse Casp2 [12366]. Constructs of wt and mutated mouse Casp2 [12366] EX8 - INT8 - EX9 - INT9 - EX10. | In vitro splicing assay using HeLa NE and in vivo splicing in HEK293, protein overexpression and siRNA knockdown. | EMSA and UV cross-linking with recombinant protein. | |
RBM5 | AGGUAA | -5 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 22162216 | Farina B, Fattorusso R, Pellecchia M. (2011) Targeting zinc finger domains with small molecules: solution structure and binding studies of the RanBP2-type zinc finger of RBM5. Chembiochem. 12(18):2837-2845. | Synthesized sequences | Chemical shift mapping (NMR) | ||
RBM5 | GGGGGG | -10 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 22517726 | O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012) Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angew Chem Int Ed Engl. 51(22):5371-5375. | Synthesized sequences | Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein | ||
RBM5 | GGUGGU | -3 | Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 | 22517726 | O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012) Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angew Chem Int Ed Engl. 51(22):5371-5375. | Synthesized sequences | Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein | ||
Sam68 | UUUUUUU | 6 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 17764653 | Rho J, Choi S, Jung CR, Im DS. (2007) Arginine methylation of Sam68 and SLM proteins negatively regulates their poly(U) RNA binding activity. Arch Biochem Biophys. 466(1):49-57. | Methylation of Sam68, SLM-1, SLM-2 proteins markedly reduced their poly(rU) binding ability in vitro. | Homopolymers | Immunoblot analysis with 293 and 293T lysates. Northwestern with recombinant protein. | |
Sam68 | UUAAAA | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 12490714 | Itoh M, Haga I, Li QH, Fujisawa J. (2002) Identification of cellular mRNA targets for RNA-binding protein Sam68. Nucleic Acids Res. 30(24):5452-5464. | synthesized oligos | Differential display and cDNA-representational difference analysis (cDNA-RDA) in vitro. In vivo coimmunoprecipitation. Further in vitro binding: protein incubated with labeled RNA. | ||
Sam68 | CAAAAU | 2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | GAAAAC | 2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | UUUUUU | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 12490714 | Itoh M, Haga I, Li QH, Fujisawa J. (2002) Identification of cellular mRNA targets for RNA-binding protein Sam68. Nucleic Acids Res. 30(24):5452-5464. | synthesized oligos | Differential display and cDNA-representational difference analysis (cDNA-RDA) in vitro. In vivo coimmunoprecipitation. Further in vitro binding: protein incubated with labeled RNA. | ||
Sam68 | UUUUA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 20186123 | Pedrotti S, Bielli P, Paronetto MP, Ciccosanti F, Fimia GM, Stamm S, Manley JL, Sette C. (2010) The splicing regulator Sam68 binds to a novel exonic splicing silencer and functions in SMN2 alternative splicing in spinal muscular atrophy. EMBO J. 29(7):1235-1247. | Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HeLa, HEK293T, SH-SY5Y and SW480 | RNA pull-down assays with HEK293T NE, UV cross-linking and immunoprecipitation with HeLa and HEK293T NE, mutation analysis, EMSA and Western blot with HEK293T NE. | |
Sam68 | UUAAAU | 10 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | AUAAAA | 10 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | CUAAAU | 10 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | CUAAAA | 10 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | UUUUAC | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | AUUUAA | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | AUUUAC | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 9341174 | Lin Q, Taylor SJ, Shalloway D. (1997) Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains. J Biol Chem. 272(43):27274-27280. | Sequences of 40 nt random for SELEX. | SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein. | ||
Sam68 | CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 20186122 | Sellier C, Rau F, Liu Y, Tassone F, Hukema RK, Gattoni R, Schneider A, Richard S, Willemsen R, Elliott DJ, Hagerman PJ, Charlet-Berguerand N. (2010) Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients. EMBO J. 29(7):1248-1261. | Sequences deriving from FMR1 [2332]. | FISH/IF and immunohistochemistry in human brain (hippocampal sections) of FXTAS patients. | ||
Sam68 | AAAAUU | 5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 20876280 | Valacca C, Bonomi S, Buratti E, Pedrotti S, Baralle FE, Sette C, Ghigna C, Biamonti G. (2010) Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene. J Cell Biol. 191(1):87-99. | Construct of SRSF1 [6426] EX4 - INT4 - EX5. Sequences deriving from SRSF1 [6426]. | In vivo splicing in SW480 cells, using WT or mutated minigenes | Mutational analysis and pull-down assay with HeLa nuclear extract. In vivo RNA immunoprecipitation of target RNA in cross-linked SW480 cells. | |
Sam68 | AUUAAA | -10 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | UAAU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | AAAUAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | AAUAAU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | AUAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | UUAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | CUAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | AAAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | AUAAU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
Sam68 | UUAAUUAAUUAAUUAACUAACUAACUAACUUUAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 23637638 | Ehrmann I, Dalgliesh C, Liu Y, Danilenko M, Crosier M, Overman L, Arthur HM, Lindsay S, Clowry GJ, Venables JP, Fort P, Elliott DJ. (2013) The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain. PLoS Genet. 9(4):e1003474. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | EMSA with recombinant protein | |
Sam68 | AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUUAAUUAAUUAAUUAACCCACCCACCCACUUUAAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | UAAAAUAAAAUAAAAUAAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | ACAGUUUAAAAUUUGAUAAAAUUU | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | UUACAUUUAAAAGAUGAUUUAAAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUCCAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUUAAUCCAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUUAAUUAAUCCAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUUAAUUAAUUAAUCCACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUUAAUUAAUUAAUUAACUAACUAACUAACUUCCAAAACACGAUCUUAAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUCCAA | -3 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
Sam68 | AAAAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAAAAAUAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAAU | -2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAAUA | -2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAAUAU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAAUUU | -2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAUA | -2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAUAA | -2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAUAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAUAUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AAUUUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AUAA | -2 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AUUAAUUA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | AUUUUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAAAUUUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAAU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAAUUUUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAUA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAUAAUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAUAUUUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAUUUUUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAUAAAUU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAUUAAU | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UUUAAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UUUAAAUAA | -5 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
Sam68 | UAAAAGCGCCCGGUUUAAA | -10 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26695943 | Rai DK, Lawrence P, Kloc A, Schafer E, Rieder E. (2015) Analysis of the interaction between host factor Sam68 and viral elements during foot-and-mouth disease virus infections. Virol J. 12:224. | Sequences deriving from wt and mutated IRES of FMDV (foot-and-mouth disease virus) | EMSA with recombinat protein | ||
Sam68 | UACGAGCGCCCGGUUUACG | -1 | Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. | 26695943 | Rai DK, Lawrence P, Kloc A, Schafer E, Rieder E. (2015) Analysis of the interaction between host factor Sam68 and viral elements during foot-and-mouth disease virus infections. Virol J. 12:224. | Sequences deriving from wt and mutated IRES of FMDV (foot-and-mouth disease virus) | EMSA with recombinat protein | ||
SAP155 | GAGGGAGGCAGGCGACGAG | -5 | Gene Name and Synonymous: SF3B1, splicing factor 3b, subunit 1, 155kDa, PRP10, PRPF10, SAP155, SF3b155, SF3B1. SAP155 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 in response to ceramide (PMID: 16790528). | 16790528 | Massiello A, Roesser JR, Chalfant CE. (2006) SAP155 Binds to ceramide-responsive RNA cis-element 1 and regulates the alternative 5' splice site selection of Bcl-x pre-mRNA. FASEB J. 20(10): 1680-1682. | Bcl-X [BCL2L1, 598] RNAs. | Mass spectrometric analysis, EMSA supershift, RNAi, pull-down assay, western immunoblotting with A549 nuclear extract. | ||
SAP155 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: SF3B1, splicing factor 3b, subunit 1, 155kDa, PRP10, PRPF10, SAP155, SF3b155, SF3B1. SAP155 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 in response to ceramide (PMID: 16790528). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
SAP155 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: SF3B1, splicing factor 3b, subunit 1, 155kDa, PRP10, PRPF10, SAP155, SF3b155, SF3B1. SAP155 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 in response to ceramide (PMID: 16790528). | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
SC35 | AGAAG | 7 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
SC35 | AGCAG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
SC35 | AGGAG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
SC35 | AGUAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11847131 | Caputi M, Zahler AM. (2002) SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J. 21(4): 845-855. | Construct of HIV-1 env [155971] EX_6D and part of flanking introns. | In vitro splicing with HeLa nuclear extracts | RNA affinity chromatography assay and immunoblot. | |
SC35 | AAGCAGUAGGG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AAGCAGUAGUC | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | ACGAGAG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | ACGAGAU | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGAGAUGCUG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGGAGAA | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGGAGAG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGGAGAU | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGGCAGU | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGGCAGUAGUC | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGGGUAU | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGUCGAU | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGUUCCAGCC | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AGUUCCAGGG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AUGAAUGCUG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGAACGAGAU | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | CGUGAUGCUG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GAGCAGUAGGG | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GAGCAGUAGUC | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GAGCAGUGGUC | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GAUGAUGCUG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GAUUCCAGCUA | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GAUUCCUGCUA | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GCGCAGUAGGG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GCGCAGUAGUC | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGAACCAGCUA | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGAAUGCCG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGAAUGCUG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGAGCC | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGCAGUAGGG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGUAGCUG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGUAUGCCG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGUAUGCUG | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGGUGAGCUG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGUGAUGCGU | 2 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGUUCCAGAU | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGUUCCAGUU | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GGUUCCUGUUA | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GUAACCAGCUA | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GUAUCCCGCUA | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GUGCAGUAGGG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GUUACCAGUCC | 2 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | GUUACGUGUAA | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UCGAGAU | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UCGUAAGCUG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGAUCGAGUA | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGGAGAU | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGGCAGUAGGG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCCAGAA | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCCAGAU | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCCAGCU | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCCAGUG | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCCGGAU | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCGAGAA | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCGAGAU | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCGAGUA | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | UGUUCGUGCG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SC35 | AAAAGAGAAG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SC35 | GAGGAGGA | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SC35 | GGAGGAC | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SC35 | GGAGGAG | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SC35 | GUAUUCUA | 7 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCCUCC | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCCCCUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGAUGGAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GACCGGUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | UGUUACUA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GACUAGAA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | AGCCUCAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCCCACA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGACGCUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | CGCUGCUA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GUUUCGAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GAGCACUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGUCGCCG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGUUAAUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCUGAUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCUCGUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGUCAGUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GAAUACCG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCUCCAA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGACUGUA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GACUCAAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GAUCCCCG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGAUCCGG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGUUGUUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GUCCUCCG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GAUCGCUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGCCGCAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGUUGGCG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | AGCUCCCA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GGACCGUA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GUCCUCAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GUCCCCUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GUCUAACG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | CGCCCUUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GUUCUGUA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GACCUGCG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | UGCUGUU | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9858550 | Schaal TD, Maniatis T. (1999) Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA. Mol Cell Biol. 19(1): 261-73. | Synthesized oligos for UV-crosslink. Construct of beta-globin [3043] EX1 - INT1 - EX2. Construct and mutants of beta-globin [3043] EX3 - INT3 -partial_EX4 - EX2 for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | UV crosslink and SDS-PAGE with HeLa S100 and nuclear extracts. | |
SC35 | UGCGGUC | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
SC35 | UGCCGCC | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
SC35 | ACGAGAGUU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGAAGCGGA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCAGAGAU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCAGAGGA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCAGAGUA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCAGUGUU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCCGAGAA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCGGAGAA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGAAUA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGAAUG | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGAUAA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGCGUG | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGUAUC | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGUGAC | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGCGAGCA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGCGAGUA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGCGUGUU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | CGGAGAGAA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GACGGAGUA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GAUGGAGGA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GCUCGAGUA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUACGAGGA | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUUCCAGAU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUUCCAGGU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUUCCAGUU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUUCGAGUG | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUUCGAGUU | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | UGCAGAGUG | 3 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGGAGAGGU | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | AGCAGAGUU | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUUCGAGUA | 10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SC35 | GUAAGUACGC | 8 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 14703516 | Zahler AM, Damgaard CK, Kjems J, Caputi M. (2004) SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. J Biol Chem. 279(11): 10077-84. | Constructs of HIV-1 Tat [155871] EX2. | In Vitro Splicing with HeLa S100 or nuclear extracts. | RNA Affinity Chromatography Assays, SDS-PAGE in HeLa cell nuclear extracts, Nuclear Extract Depletion by high affinity RNA. SDS-PAGE, immunoblotting, RNA Footprinting Analysis. | |
SC35 | CUAGACUAGA | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 14703516 | Zahler AM, Damgaard CK, Kjems J, Caputi M. (2004) SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. J Biol Chem. 279(11): 10077-84. | Constructs of HIV-1 Tat [155871] EX2. | In Vitro Splicing with HeLa S100 or nuclear extracts. | RNA Affinity Chromatography Assays, SDS-PAGE in HeLa cell nuclear extracts, Nuclear Extract Depletion by high affinity RNA. SDS-PAGE, immunoblotting, RNA Footprinting Analysis. | |
SC35 | AUCCCGUG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11779509 | Zhu J, Mayeda A, Krainer AR. (2001) Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell. 8(6): 1351-1361. | Sequence of HIV-1 Tat [155871] EX3. | In Vitro Splicing with HeLa S100 and HeLa nuclear extracts. | UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot. | |
SC35 | CACCUCCG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11779509 | Zhu J, Mayeda A, Krainer AR. (2001) Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell. 8(6): 1351-1361. | Sequence of HIV-1 Tat [155871] EX3. | In Vitro Splicing with HeLa S100 and HeLa nuclear extracts. | UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot. | |
SC35 | GAAUCCCG | 6 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11779509 | Zhu J, Mayeda A, Krainer AR. (2001) Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell. 8(6): 1351-1361. | Sequence of HIV-1 Tat [155871] EX3. | In Vitro Splicing with HeLa S100 and HeLa nuclear extracts. | UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot. | |
SC35 | GAUUAGUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11779509 | Zhu J, Mayeda A, Krainer AR. (2001) Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell. 8(6): 1351-1361. | Sequence of HIV-1 Tat [155871] EX3. | In Vitro Splicing with HeLa S100 and HeLa nuclear extracts. | UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot. | |
SC35 | GGAGGA | -5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
SC35 | GAAGAAGA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 14729981 | Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004) RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon. Mol Cell Biol. 24(3):1387-1400. | Sequences of fibronectin FN1 [2335] EX_EDA and mutants | UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts | ||
SC35 | AGGAGGA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | CGCAGGU | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | GACCCGG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | CGCAGGG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | CAGAGGU | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SC35 | AGCUGUU | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9858550 | Schaal TD, Maniatis T. (1999) Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA. Mol Cell Biol. 19(1): 261-73. | Synthesized oligos for UV-crosslink. Construct of beta-globin [3043] EX1 - INT1 - EX2. Construct and mutants of beta-globin [3043] EX3 - INT3 -partial_EX4 - EX2 for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | UV crosslink and SDS-PAGE with HeLa S100 and nuclear extracts. | |
SC35 | CAGUAGA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 14703516 | Zahler AM, Damgaard CK, Kjems J, Caputi M. (2004) SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. J Biol Chem. 279(11): 10077-84. | Constructs of HIV-1 Tat [155871] EX2. | In Vitro Splicing with HeLa S100 or nuclear extracts. | RNA Affinity Chromatography Assays, SDS-PAGE in HeLa cell nuclear extracts, Nuclear Extract Depletion by high affinity RNA. SDS-PAGE, immunoblotting, RNA Footprinting Analysis. | |
SC35 | GAUUGAUG | -5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 19843576 | Chandradas S, Deikus G, Tardos JG, Bogdanov VY. (2010) Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 87(1):147-152. | In this context, SRp40 and SC35 antagonize other SR proteins by competing for certain sites in exon 5, thereby promoting TF (tissue factor) exon 5 exclusion. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. |
SC35 | GAUUCCCU | -5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 19843576 | Chandradas S, Deikus G, Tardos JG, Bogdanov VY. (2010) Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 87(1):147-152. | In this context, SRp40 and SC35 antagonize other SR proteins by competing for certain sites in exon 5, thereby promoting TF (tissue factor) exon 5 exclusion. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. |
SC35 | CCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC | -5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9130721 | Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997) The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A. EMBO J. 16(7):1772-1784. | Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. | In vitro splicing with HeLa NE | In vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins. | |
SC35 | GAUCCCUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU | -5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 9130721 | Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997) The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A. EMBO J. 16(7):1772-1784. | Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. | In vitro splicing with HeLa NE | In vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins. | |
SC35 | AGAGGAAGGCGA | 7 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
SC35 | AGGAGCAGGGGACGAAG | 7 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
SC35 | GAAGAAGAA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
SC35 | AGGGUUGAGGGGAGCAGGGU | 7 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
SC35 | GGUGGGGCCGGGGCCAAGG | -2 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 15798212 | Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005) The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis. Mol Cell Biol. 25(8):3286-3294. | Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8 | In vitro splicing in HeLa NE | RNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA . | |
SC35 | GGUGGGGCCGGAGCCAAGG | -10 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 15798212 | Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005) The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis. Mol Cell Biol. 25(8):3286-3294. | Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8 | In vitro splicing in HeLa NE | RNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA . | |
SC35 | GAGGAG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 16990281 | Hallay H, Locker N, Ayadi L, Ropers D, Guittet E, Branlant C. (2006) Biochemical and NMR study on the competition between proteins SC35, SRp40, and heterogeneous nuclear ribonucleoprotein A1 at the HIV-1 Tat exon 2 splicing site. J Biol Chem. 281(48):37159-37174. | Sequences deriving from HIV-1 Tat [155871] | Competition assays with recombinant protein | ||
SC35 | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
SC35 | CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
SC35 | UGUGUCACUGUCUGGUUC | 1 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SC35 | GGCAGGAAGAAGAGGAGCA | 7 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SC35 | CCAGACACCGGAAACCCCUGCCACACCAC | 4 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SC35 | GGGACCUGCAGAGACUGUAA | 5 | Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264. SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664). | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
SF1 | UACUAAC | -10 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9182766 | Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 89(5):781-787. | 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. | EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences. | ||
SF1 | UGCUAAC | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9182766 | Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 89(5):781-787. | 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. | EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences. | ||
SF1 | GACUAAC | -6 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9182766 | Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 89(5):781-787. | 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. | EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences. | ||
SF1 | UACUGAC | -4 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9182766 | Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 89(5):781-787. | 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. | EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences. | ||
SF1 | UACUAAG | -4 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9182766 | Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 89(5):781-787. | 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. | EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences. | ||
SF1 | UAGUAAG | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9182766 | Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997) The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 89(5):781-787. | 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. | EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences. | ||
SF1 | UGCUGAC | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9512519 | Berglund JA, Abovich N, Rosbash M. (1998) A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. Genes Dev. 12(6):858-867. | Synthesized sequences | Footprinting assay, EMSA, competition assay with purified protein | ||
SF1 | UGCUGCC | -1 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 9512519 | Berglund JA, Abovich N, Rosbash M. (1998) A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. Genes Dev. 12(6):858-867. | Synthesized sequences | Footprinting assay, EMSA, competition assay with purified protein | ||
SF1 | UACUAAU | -10 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 11438677 | Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001) Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex. Mol Cell Biol. 21(15):5232-5241. | Synthesized sequences | Reporter gene assay using recombinant protein | ||
SF1 | UAGUAAC | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 11438677 | Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001) Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex. Mol Cell Biol. 21(15):5232-5241. | Synthesized sequences | Reporter gene assay using recombinant protein | ||
SF1 | UACUAGC | -2 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 11438677 | Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001) Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex. Mol Cell Biol. 21(15):5232-5241. | Synthesized sequences | Reporter gene assay using recombinant protein | ||
SF1 | UACGAAC | -1 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 11438677 | Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001) Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex. Mol Cell Biol. 21(15):5232-5241. | Synthesized sequences | Reporter gene assay using recombinant protein | ||
SF1 | ACAGUCA | -2 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 11438677 | Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001) Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex. Mol Cell Biol. 21(15):5232-5241. | Synthesized sequences | Reporter gene assay using recombinant protein | ||
SF1 | CACUGAC | -7 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 15496424 | Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004) Branch site haplotypes that control alternative splicing. Hum Mol Genet. 13(24):3189-3202. | Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 | In vitro splicing in HeLa and HEK293T nuclear extracts. | EMSA with recombinant protein. | |
SF1 | CGCUGAC | -7 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 15496424 | Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004) Branch site haplotypes that control alternative splicing. Hum Mol Genet. 13(24):3189-3202. | Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 | In vitro splicing in HeLa and HEK293T nuclear extracts. | EMSA with recombinant protein. | |
SF1 | CACCGAC | -1 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 15496424 | Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004) Branch site haplotypes that control alternative splicing. Hum Mol Genet. 13(24):3189-3202. | Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 | In vitro splicing in HeLa and HEK293T nuclear extracts. | EMSA with recombinant protein. | |
SF1 | CACAGAC | -1 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 15496424 | Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004) Branch site haplotypes that control alternative splicing. Hum Mol Genet. 13(24):3189-3202. | Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 | In vitro splicing in HeLa and HEK293T nuclear extracts. | EMSA with recombinant protein. | |
SF1 | AUUAAC | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
SF1 | ACUUAU | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
SF1 | ACUAAU | -5 | Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636. Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453) | 24722255 | Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014) The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing. PLoS Genet. 10(4):e1004289. | Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2 | In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein. | EMSA with WT and mutated sequences and UV crosslinking with recombinant protein | |
SF2/ASF | AACACGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 15302913 | Chiodi I, Corioni M, Giordano M, Valgardsdottir R, Ghigna C, Cobianchi F, Xu RM, Riva S, Biamonti G. (2004) RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies. Nucleic Acids Res. 32(14): 4127-4136. | Construct of Adenovirus E1A for in vivo splicing. Synthesized 16nt oligos for EMSA. | In vivo splicing in HeLa cells. | EMSA. | |
SF2/ASF | AAAGAAGAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11278454 | Chung H , Derse D. (2001) Binding sites for Rev and ASF/SF2 map to a 55-nucleotide purine-rich exonic element in equine infectious anemia virus RNA. J Biol Chem. 276 (22): 18960-18967. | synthesized oligos | EMSA, UV crosslink competition, and immunoprecipitation in HeLa nuclear extracts. | ||
SF2/ASF | AAAAGAGAAG | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SF2/ASF | GAGGAGGA | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SF2/ASF | GGAGGAC | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SF2/ASF | GGAGGAG | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9848651 | Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998) Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2. RNA. 4(12): 1523-1536. | Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing. | In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells. | UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts. | |
SF2/ASF | AGGAGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SF2/ASF | CGCAGGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SF2/ASF | GACCCGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 10629063 | Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000) Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 20(3): 1063-1071. | Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. | ||
SF2/ASF | CACAGUG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAGACGU | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAGAGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAGAGGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAGAGGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAGCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAGCGGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGCAGGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGCUCGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGGACGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGGACGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGGAGGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGGCCGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGGCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CUCAGGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CUCCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CUGACUA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GACAGGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | AGACAGAGAC | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 12419255 | Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, Branlant C. (2002) A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding. J Mol Biol. 323(4): 629-652. | Constructs of HIV-1 A7 3' splice site | In vitro splicing with HeLa cell nuclear or cytoplasmic S100 extracts. | Competition assay, UV crosslink, immunoprecipitation with HeLa nuclear extracts, EMSA and supershift assays. | |
SF2/ASF | GAAGAAGAA | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 12419255 | Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, Branlant C. (2002) A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding. J Mol Biol. 323(4): 629-652. | Constructs of HIV-1 A7 3' splice site | In vitro splicing with HeLa cell nuclear or cytoplasmic S100 extracts. | Competition assay, UV crosslink, immunoprecipitation with HeLa nuclear extracts, EMSA and supershift assays. | |
SF2/ASF | AGAAGAAC | 6 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGACAGAGC | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACAAGGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACGCGCC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGAAGGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGAUGGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGAAAGACC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGACGACGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGACUGAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGAGAAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGAGGAU | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGAGGUAAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AUGACAGAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AUGACUGAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | CCGCGCA | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | CGAAGGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | CGGACAGAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | CGGACGAAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GACGAAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GAUACGAAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GCGCGCA | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GGAAAGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GGAACAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GGAAUGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GGACGAAU | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GGAUGAAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GUGACAGAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UAGACAGAGC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UCGCACA | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UCGGGCA | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UGAAGAAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UGAUGAAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UGUAGGAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACGCGAG | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACGCGCU | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AUGACAGAAC | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | CAGACAGAGC | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GAGGAAC | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GGAGGAAC | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UAGACAGAAC | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UGGACAGAGC | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACGCGCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACGCUCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGACGGAAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GAGACAGAGC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | UUGACAGAGC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | ACGCGCA | 7 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGAAGAGC | 7 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | AGGACGAAGC | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7543047 | Tacke R, Manley JL. (1995) The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 14(14): 3540-3551. | Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa nuclear and S100 extracts. | SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract. | |
SF2/ASF | GAGGAAGAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 7651409 | Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995) A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer. Mol Cell Biol. 15(9):4898-4907. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assay, immunoprecipitation | |
SF2/ASF | AAGGUG | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 12612063 | Rooke N, Markovtsov V, Cagavi E, Black DL. (2003) Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1. Mol Cell Biol. 23(6):1874-1884. | Construct of c-src [20779] EX_N1. | In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts. | UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts. | |
SF2/ASF | GGAAGAAGAUAAAGAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 12826680 | Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003) The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20. J Biol Chem. 278(35):32943-32953. | Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10. | In vivo splicing in SVK14 and HEK293-EBNA cells. | UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts. | |
SF2/ASF | GAUGAAGAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 10523623 | Bourgeois CF, Popielarz M, Hildwein G, Stevenin J. (1999) Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation. Mol Cell Biol. 19(11):7347-7356. | Construct and variants of Adenovirus E1A unit. | in vitro splicing with Hela nuclear extracts, in vivo splicing in Hela cells, in vitro complementation assays. | UV crosslink, immunoprecipitation. | |
SF2/ASF | AUCCAGGAGGGGAACAGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11751603 | Smith PJ, Spurrell EL, Coakley J, Hinds CJ, Ross RJ, Krainer AR, Chew SL. (2002) An exonic splicing enhancer in human IGF-I pre-mRNA mediates recognition of alternative exon 5 by the serine-arginine protein splicing factor-2/alternative splicing factor. Endocrinology. 143(1):146-154. | Construct of IGF1 [3479] EX4 - INT4 - EX5. | In vitro splicing with HeLa nuclear extracts and cotransfection | Immunoprecipitation. | |
SF2/ASF | CAGACAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11925564 | Cartegni L, Krainer AR. (2002) Disruption of an SF2/ASF-dependent exonic splicing enhancer in SMN2 causes spinal muscular atrophy in the absence of SMN1. Nat Genet. 30(4):377-384. | Construct and mutants of SMN1 [6606] and SMN2 [6607] EX6 - shortened_INT6 - EX7 - INT7 -shortened_EX8. | In vivo splicing in 293-HEK. | UV crosslink, immunoprecipitation, SDS-PAGE. | |
SF2/ASF | GAAGAAGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 14729981 | Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004) RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon. Mol Cell Biol. 24(3):1387-1400. | Sequences of fibronectin FN1 [2335] EX_EDA and mutants | UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts | ||
SF2/ASF | CAACCGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CACAGCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CACAGCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CACGGAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CAGUCGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCAACCC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCAACCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCAACGC | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCAAUGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCACAGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCACGCU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCACGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCACUGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCAUGGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCAGCC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCAUGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCCGAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCCGCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCGACU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCGUUU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCUGAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCCUGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGACAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGACCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGACGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGAGCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGAUGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGCUAU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCGGACG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCUAGGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CCUCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGACGCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGACGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGAGCGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGAGGCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGAUGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCCGGA | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCCGUA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGCAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGCCC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGGAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGGAC | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGGAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGGUU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGCGUCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGGAGCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGGAGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGGCACA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CGGCAGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CUACGAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CUCGUGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | CUGAACA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | GCCCACU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | GCCGGAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | UGAACCA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16825284 | Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006) An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers. Hum Mol Genet. 15(16):2490-2508. | Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein. | Functional SELEX of random 7nt and 14nt with recombinant protein. | |
SF2/ASF | GACUGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GAGACGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGCACGG | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGGACGU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GUGACGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | AUAGGACUGGAUCGAGUUGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | AUCGGACAGGGUCCAGCAGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | AUCGGCCGAUCUGUGAGUUA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | AUGCUCCGGAAUCGGAACGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CAAGCACAGUGACCGAGAAC | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CCAGAGGGCGGAAACGUUGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGAUGACCCUCAGACGUAUA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGAUGUCCCGGAGGUUUUGC | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGCCGGACGACGUGUGUUG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGCGGUUAGGAGGAUGGAAA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGUCGCAGGGCAGGUGGGAA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGUGAAACUGCCCAGAGGUG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CGUGCCCACGUGUCUCAGGU | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | CUCCAGACGUCGUUUGUUGC | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GACGUCCAGUACGCUCGAGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GCGGACCCGGAAAGGACUAA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGAAGUACGGGACGUGCCGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGCACGGCGAGACACCAUCA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGCACGGGGAGGCACCAUCA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGCAGAGGAGAGCCGGGACG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGCAGCGGGCGUACCCGGAU | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGCUUGGUUCGCGGUGACGA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GGUCGCAGGUCAGGUGGGUU | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GUGGGUUCGGCGGAAUCAAG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | GUUGCGGAGACGACCCGAGC | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | UGACAGCGGAAGGUACAGUG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | UGAGUGCGCGGAUAGACUGACUA | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | UUCGGACGGGCUAGGGAUGG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SF2/ASF | UGGAAAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 17144911 | Kammler S, Otte M, Hauber I, Kjems J, Hauber J, Schaal H. (2006) The strength of the HIV-1 3' splice sites affects Rev function. Retrovirology. 3:89. | Constructs of HIV-1 Tat [155871] EX2. | In vivo splicing in HeLa-T4+ cells. | Mutational analysis and pull-down assay and immunoblot with HeLa nuclear extract. | |
SF2/ASF | UAUUACGGAAU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19111562 | Goncalves V, Theisen P, Antunes O, Medeira A, Ramos JS, Jordan P, Isidro G. (2009) A missense mutation in the APC tumor suppressor gene disrupts an ASF/SF2 splicing enhancer motif and causes pathogenic skipping of exon 14. Mutat Res. 662(1-2): 33-36. | In this context, SF2/ASF and SRp55 do not bind UAUUAGGGAAU. In addiction, SRp55 does not bind UAUUACGGAAU. | Construct of APC [324] INT13 - EX14 - INT14. | In vivo splicing in DLD-1 or HT29 colorectal cells. | RNA interference. |
SF2/ASF | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
SF2/ASF | GCGAGCG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SF2/ASF | GAGCGAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SF2/ASF | GAGCGGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SF2/ASF | AGCGAGC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SF2/ASF | CGGAAGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18315555 | Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008) SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. J Thromb Haemost. 6(5):877-884. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 and SC cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. | |
SF2/ASF | CAGACAG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18315555 | Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008) SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. J Thromb Haemost. 6(5):877-884. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 and SC cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. | |
SF2/ASF | GGAUAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | GCAUAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | GGAUUC | 7 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | GGGUAC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | GGAUAU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | CUGAUUUGUAUUUAUUAGACUC | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | AACAGAAAAAGAAAUAUUU | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | CUGAUUUGUACCUAUUAGAUUC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | UACUGAAGAACAAGUAUUU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SF2/ASF | CCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9130721 | Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997) The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A. EMBO J. 16(7):1772-1784. | Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. | In vitro splicing with HeLa NE | In vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins. | |
SF2/ASF | GAUCCCUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9130721 | Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997) The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A. EMBO J. 16(7):1772-1784. | Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. | In vitro splicing with HeLa NE | In vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins. | |
SF2/ASF | AGGGAAAGACAGGGAGGGAGAGAGAAAGAGAAAGGGACU | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9130721 | Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997) The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A. EMBO J. 16(7):1772-1784. | Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. | In vitro splicing with HeLa NE | In vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins. | |
SF2/ASF | AGAGCAGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 9826658 | Zheng ZM, Huynen M, Baker CC. (1998) A pyrimidine-rich exonic splicing suppressor binds multiple RNA splicing factors and inhibits spliceosome assembly. Proc Natl Acad Sci U S A. 95(24):14088-14093. | BPV-1 sequences. | UV cross-linking and immunoprecipitation with HeLa NE | ||
SF2/ASF | AGAGGAAGGCGA | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
SF2/ASF | AGGAGCAGGGGACGAAG | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11096110 | Lejeune F, Cavaloc Y, Stevenin J. (2001) Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor. J Biol Chem. 276(11):7850-7858. | Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4. | In vitro splicing with HeLa extracts. | UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts. | |
SF2/ASF | ACAACAACUAGCCACGGAUCAU | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11336712 | Huang Y, Steitz JA. (2001) Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA. Mol Cell. 7(4):899-905. | Sequences wt and mutated deriving from mouse Histone 2A | UV cross-linking and immunoprecipitation with HeLa NE | ||
SF2/ASF | GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11421362 | Hastings ML, Wilson CM, Munroe SH. (2001) A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA. RNA. 7(6):859-874. | Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10 | In vitro splicing with HeLa NE | UV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins. | |
SF2/ASF | GGGGACCCGACAGGCCCGAAGGAAUAGAAGAAGAAGGUGGAGAGAGAGACAGAGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11575921 | Tange TO, Kjems J. (2001) SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain. J Mol Biol. 312(4):649-662. | Constructs of wt and mutants of Tat [155871] EX2 - INT2 - EX3 | In vitro splicing with HeLa NE and recombinant protein | EMSA with recombinant protein, UV cross-linking with HeLa NE. | |
SF2/ASF | AGGGUUGAGGGGAGCAGGGU | 7 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 15208309 | Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004) hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B. J Biol Chem. 279(37):38249-38259. | Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7. | In vitro splicing in HeLa NE | EMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE | |
SF2/ASF | GGUGGGGCCGGGGCCAAGG | -5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 15798212 | Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005) The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis. Mol Cell Biol. 25(8):3286-3294. | Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8 | In vitro splicing in HeLa NE | RNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA . | |
SF2/ASF | GGUGGGGCCGGAGCCAAGG | -5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 15798212 | Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005) The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis. Mol Cell Biol. 25(8):3286-3294. | Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8 | In vitro splicing in HeLa NE | RNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA . | |
SF2/ASF | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
SF2/ASF | GGUUUCAGACAAAAUCA | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16385450 | Cartegni L, Hastings ML, Calarco JA, de Stanchina E, Krainer AR. (2006) Determinants of exon 7 splicing in the spinal muscular atrophy genes, SMN1 and SMN2. Am J Hum Genet. 78(1):63-77. | Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 | In vitro splicing in HeLa | RNA affinity chromatography with HeLa NE, western blot. | |
SF2/ASF | UAUGGAGGAUUCACUGUACAGAAUGAAGCCAACAAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 16611940 | Spena S, Tenchini ML, Buratti E. (2006) Cryptic splice site usage in exon 7 of the human fibrinogen Bbeta-chain gene is regulated by a naturally silent SF2/ASF binding site within this exon. RNA. 12(6):948-958. | Sequences deriving from FGB [2244] | UV cross-linking, immunoprecipitation in HeLa NE | ||
SF2/ASF | CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
SF2/ASF | CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 17337441 | Schaub MC, Lopez SR, Caputi M. (2007) Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes. J Biol Chem. 282(18):13617-13626. | Synthesized sequences | RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein. | ||
SF2/ASF | UUACAUGAGCAUUAGGAGA | -5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 17576688 | Buratti E, Stuani C, De Prato G, Baralle FE. (2007) SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer. Nucleic Acids Res. 35(13):4359-4368. | Sequences deriving from CFTR [1080]. Constructs of CFTR [1080] INT8 - EX9 - INT9. | In vivo splicing in Hep3B | UV cross-linking, immunoprecipitation with HeLa NE | |
SF2/ASF | CACACGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 17668007 | Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA. (2007) Structural and functional analysis of RNA and TAP binding to SF2/ASF. EMBO Rep. 8(8):756-762. | Synthesized oligos | UV cross-linking, chemical shift mapping (NMR) with recombinant protein. | ||
SF2/ASF | GCACCUGAUGGUGAAGAAGACACUGCAGAGC | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
SF2/ASF | UGCAGAUGCUGAGUUUGUGU | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
SF2/ASF | UGCAGAUGCUUAGUUUGUGU | 3 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18391021 | Goina E, Skoko N, Pagani F. (2008) Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant. Mol Cell Biol. 28(11):3850-3860. | Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. | In vivo splicing in HeLa. | Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA. | |
SF2/ASF | GGCAGGAAGAAGAGGAGCA | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SF2/ASF | CCAGACACCGGAAACCCCUGCCACACCAC | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SF2/ASF | AAAGGACAAAGGACAAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 8769651 | Lynch KW, Maniatis T. (1996) Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer. Genes Dev. 10(16):2089-2101. | Sequences deriving from D. melanogaster dsx [40940]. | UV-cross link, immunoprecipitation with HeLa extracts. | ||
SF2/ASF | GACGACGAGGAGCAGCAG | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11454855 | Tian H, Kole R. (2001) Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein. J Biol Chem. 276(36):33833-33839. | Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2). | In vitro splicing with HeLa NE | Filter binding assay, UV cross-linking and immunoprecipitation with HeLa NE | |
SF2/ASF | GACGACUCAGCAG | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 11454855 | Tian H, Kole R. (2001) Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein. J Biol Chem. 276(36):33833-33839. | Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2). | In vitro splicing with HeLa NE | Filter binding assay, UV cross-linking and immunoprecipitation with HeLa NE | |
SF2/ASF | GUUGGAGAAACGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19602482 | Goncalves V, Matos P, Jordan P. (2009) Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 18(19):3696-3707. | Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4. | In vivo splicing in HT29 | EMSA with recombinant protein and EMSA supershift with DLD-1 NE | |
SF2/ASF | GUUGGACAAAUGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC | 1 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19602482 | Goncalves V, Matos P, Jordan P. (2009) Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 18(19):3696-3707. | Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4. | In vivo splicing in HT29 | EMSA with recombinant protein and EMSA supershift with DLD-1 NE | |
SF2/ASF | GUUGGAGAAACGUACGGCAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19602482 | Goncalves V, Matos P, Jordan P. (2009) Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 18(19):3696-3707. | Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4. | In vivo splicing in HT29 | EMSA with recombinant protein and EMSA supershift with DLD-1 NE | |
SF2/ASF | CAAAAAGAAGGAAGGUGCUCACAU | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19953646 | Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010) A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy. Hum Mutat. 31(1):E1110-1125. | Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein | |
SF2/ASF | CAAAAAGAACGAAGGUGCUCACAU | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 19953646 | Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010) A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy. Hum Mutat. 31(1):E1110-1125. | Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293 | UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein | |
SF2/ASF | AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG | 2 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
SF2/ASF | AUUUUCCUUACAGGGUUUCAGACAAAAUCAAAAAG | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 18794368 | Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008) The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene. Mol Cell Biol. 28(22):6929-6938. | Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8. | In vivo splicing in HEK293. | RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein. | |
SF2/ASF | GAAUGGCCGGAGGAGAUUCAGCCUGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 20120036 | Homolova K, Zavadakova P, Doktor TK, Schroeder LD, Kozich V, Andresen BS. (2010) The deep intronic c.903+469T>C mutation in the MTRR gene creates an SF2/ASF binding exonic splicing enhancer, which leads to pseudoexon activation and causes the cblE type of homocystinuria. Hum Mutat. 31(4):437-444. | Sequences deriving from wt or mutated MTRR [4552]. Constructs of wt or mutated HBB [3043]_EX1 - MTRR [4552]_INT6 - HBB [3043]_EX2 and Tat [155871]_EX1 - MTRR [4552]_INT6 - Tat [155871]_EX2. | In vivo splicing in HEK293, protein overexpression and siRNA knockdown | Pulldown and Western blotting with HeLa NE | |
SF2/ASF | UGCUUUGGUUAUAUUUAGCUCCAAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 20118245 | Moulton VR, Tsokos GC. (2010) Alternative splicing factor/splicing factor 2 regulates the expression of the zeta subunit of the human T cell receptor-associated CD3 complex. J Biol Chem. 285(17):12490-12496. | Sequences deriving from CD247 [919] and synthesized sequences | In vivo splicing in T cells overexpressing SF2/ASF | EMSA supershift with human T cells NE or recombinant protein | |
SF2/ASF | GGGACCUGCAGAGACUGUAA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 22434879 | Zubovic L, Baralle M, Baralle FE. (2012) Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands. Nucleic Acids Res. 40(13):6255-6269. | Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19 | In vivo splicing in HeLa | Pulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa. | |
SF2/ASF | ACGCCCUCUGCUGGAACUGA | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 20122404 | Motta-Mena LB, Heyd F, Lynch KW. (2010) Context-dependent regulatory mechanism of the splicing factor hnRNP L. Mol Cell. 37(2):223-234. | Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7. | In vitro splicing in JSL1 NE | Mutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE. | |
SF2/ASF | GGGGCCGGGGCCGGGGCCGGGGCC | 8 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 23423380 | Reddy K, Zamiri B, Stanley SY, Macgregor RB Jr, Pearson CE. (2013) The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures. J Biol Chem. 288(14):9860-9866. | Synthetic sequences. | EMSA with recombinant protein. | ||
SF2/ASF | AGAAGAACAGAAGAACAGAAGAAC | 4 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 23423380 | Reddy K, Zamiri B, Stanley SY, Macgregor RB Jr, Pearson CE. (2013) The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures. J Biol Chem. 288(14):9860-9866. | Synthetic sequences. | EMSA with recombinant protein. | ||
SF2/ASF | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 24371143 | Zamiri B, Reddy K, Macgregor RB Jr, Pearson CE. (2014) TMPyP4 porphyrin distorts RNA G-quadruplex structures of the disease-associated r(GGGGCC)n repeat of the C9orf72 gene and blocks interaction of RNA-binding proteins. J Biol Chem. 289(8):4653-4659. | Synthetic sequences. | EMSA with recombinant protein. | ||
SF2/ASF | CAGGUAAGAC | 5 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 8566754 | Lou H, Gagel RF, Berget SM. (1996) An intron enhancer recognized by splicing factors activates polyadenylation. Genes Dev. 10(2):208-219. | Sequences deriving from wt and mutated intron 4 of CALCA [796] | UV crosslink and immunoprecipitation | ||
SF2/ASF | GCAGUGACGACGCGCUGCUCAAGAACUACGGUCUGCUCUCCUGCUUCCGGAAGGACCUGCAUAAGACGGAGACGUACCUGAGGGUCAUGAAGUGCCGCCGCUUCGGGGAGGCCAG | 10 | Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228. The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396). | 8276242 | Sun Q, Mayeda A, Hampson RK, Krainer AR, Rottman FM. (1993) General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes Dev. 7(12B):2598-2608. | Construct of cow GH1 [280804] EX4 - INT4 - EX5 | In vitro splicing in HeLa nuclear extract, using also competing RNAs or protein excess | UV crosslinking and immunoprecipitation in HeLa nuclear extract, UV crosslinking with purified HeLa protein or recombinant protein | |
SLM-1 | UUUUUUU | 7 | Gene Name and Synonymous: KHDRBS2, KH domain containing RNA binding signal transduction associated 2, SLM1, FLJ38664, MGC26664, bA535F17.1. | 17764653 | Rho J, Choi S, Jung CR, Im DS. (2007) Arginine methylation of Sam68 and SLM proteins negatively regulates their poly(U) RNA binding activity. Arch Biochem Biophys. 466(1):49-57. | Methylation of Sam68, SLM-1, SLM-2 proteins markedly reduced their poly(rU) binding ability in vitro. | Homopolymers | Immunoblot analysis with 293 and 293T lysates. Northwestern with recombinant protein. | |
SLM-2 | UUUUUUU | 7 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 17764653 | Rho J, Choi S, Jung CR, Im DS. (2007) Arginine methylation of Sam68 and SLM proteins negatively regulates their poly(U) RNA binding activity. Arch Biochem Biophys. 466(1):49-57. | Methylation of Sam68, SLM-1, SLM-2 proteins markedly reduced their poly(rU) binding ability in vitro. | Homopolymers | Immunoblot analysis with 293 and 293T lysates. Northwestern with recombinant protein. | |
SLM-2 | AGAUAAA | 5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SLM-2 | AUAAAAC | 5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SLM-2 | UAAAUAA | 5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SLM-2 | AAAUAAA | 5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SLM-2 | AUUAAAC | 5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SLM-2 | AUUAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | UAAU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | AAAUAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | AAUAAU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | AUAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | UUAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | CUAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | AAAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | AUAAU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | ACAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | AUAAC | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 26758068 | Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016) Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68. Nat Commun. 7:10355. | Synthetic sequences. | X-ray crystallography and fluorescence polarization | ||
SLM-2 | UUAAUUAAUUAAUUAACUAACUAACUAACUUUAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 23637638 | Ehrmann I, Dalgliesh C, Liu Y, Danilenko M, Crosier M, Overman L, Arthur HM, Lindsay S, Clowry GJ, Venables JP, Fort P, Elliott DJ. (2013) The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain. PLoS Genet. 9(4):e1003474. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | EMSA with recombinant protein | |
SLM-2 | AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUUAAUUAAUUAAUUAACCCACCCACCCACUUUAAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | UAAAAUAAAAUAAAAUAAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | ACAGUUUAAAAUUUGAUAAAAUUU | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | UUACAUUUAAAAGAUGAUUUAAAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUCCAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUUAAUCCAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUUAAUUAAUCCAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUUAAUUAAUUAAUCCACUAACUAACUAACUUUAAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUUAAUUAAUUAAUUAACUAACUAACUAACUUCCAAAACACGAUCUUAAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUCCAA | -10 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 27994030 | Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016) Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control. Nucleic Acids Res. | Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20 | In vivo splicing in HEK293 cells, using WT or mutated minigenes | Fluorescence polarization | |
SLM-2 | AAAAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAAAAAUAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAAU | -2 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAAUA | -2 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAAUAU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAAUUU | -2 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAUA | -2 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAUAA | -2 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAUAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAUAUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AAUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AUAA | -2 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AUUAAUUA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | AUUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAAAUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAAU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAAUUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAUA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAUAAUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAUAUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAAUUUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAUAAAUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UAAUUAAU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UUUAAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UUUAAAUAA | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SLM-2 | UUUUUU | -5 | Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. | 24096002 | Foot JN, Feracci M, Dominguez C. (2014) Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination. Methods. 65(3):288-301. | Synthetic sequences. | X-ray crystallography, NMR spectroscopy and NMR chemical shift | ||
SRm160 | GGGAACAAAAGCUGGGUACCGGGCCCCCCCUCGA | 5 | Gene Name and Synonymous: SRRM1, serine/arginine repetitive matrix 1, POP101, SRM160, MGC39488, 160-KD. | 12600940 | Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ. (2003) Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing. Genes Dev. 17(4):461-475. | Synthesized sequences | EMSA supershift using recombinant protein | ||
SRp20 | ACAUCAUCU | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACAUCGACU | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACAUCGAUC | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACAUCGAUU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACAUCGCUU | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACAUCGUUC | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACUACGACG | 4 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACUACGAUC | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACUUCGACU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CGAUCGACU | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAACGACU | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAACGAUC | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAACGAUU | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAGAGAUU | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAUCGACA | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAUCGACU | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAUCGAUA | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAUCGAUC | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAUCGCUU | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCAUCGUUC | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCUUCGAUC | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | AACUUUAU | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | ACAUUCAU | 4 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | AUCAUCAU | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | AUCUUCAC | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | AUCUUCAU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CACAUCAU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CACUACAC | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CACUUCAG | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CACUUCAU | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CGCUUCAU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUCUUCAC | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | GACUUCAU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | GACUUCUU | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UACAUCAC | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UACUUCAA | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UUCAUCAC | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UUCAUCAU | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UUCUCCAA | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UUCUUCAA | 9 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UUCUUCAU | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CAUCAAC | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUACAAA | 8 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUACAAC | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUACAAU | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUACAGC | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUACGAC | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUUCAAC | 10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | GAUCAAC | 3 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UAUCAAC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UCACAAC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UGUCAAC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | UUACGAC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10094314 | Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999) The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 5(3): 468-483. | Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. | In vitro splicing in HeLa S100 extracts. | SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts. | |
SRp20 | CUCCGCUCCUCUUC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 9710581 | Lou H, Neugebauer KM, Gagel RF, Berget SM. (1998) Regulation of alternative polyadenylation by U1 snRNPs and SRp20. Mol Cell Biol. 18(9): 4977-85. | Construct of CALCA [796] INT4 and mutants for UV-crosslink and immunoprecipitation. Construct of CALCA EX4 - INT4 - EX5 - INT5 - EX6 fused to a heterologous first exon from adenovirus used for in vivo splicing. | In vivo splicing in T98G cells. | UV crosslink, competition, immunoprecipitation in HeLa cell nuclear extracts. EMSA with recombinant protein. | |
SRp20 | CCUCGUCC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 10022858 | Schaal TD, Maniatis T. (1999) Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 19(3): 1705-1719. | Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. | SELEX winner confermed by immublotting in Hela nuclear extracts. | |
SRp20 | GGAAGAAGAUAAAGAC | 3 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 12826680 | Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003) The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20. J Biol Chem. 278(35):32943-32953. | Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10. | In vivo splicing in SVK14 and HEK293-EBNA cells. | UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts. | |
SRp20 | UUCUUCUUCUUCUUCUUCUUCUUCUUCUUC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
SRp20 | ACAACAAGAAGACGCGCAUCAU | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 11336712 | Huang Y, Steitz JA. (2001) Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA. Mol Cell. 7(4):899-905. | Sequences wt and mutated deriving from mouse Histone 2A | UV cross-linking and immunoprecipitation with HeLa NE | ||
SRp20 | CUGCACCACCACCUGGUUC | 2 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp20 | CUGCACCACCACCUAUCUA | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp20 | CCAGACACCGGAAACCCCUGCCACACCAC | 6 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp20 | GUUGGAGAAACGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC | -10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 19602482 | Goncalves V, Matos P, Jordan P. (2009) Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 18(19):3696-3707. | Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4. | In vivo splicing in HT29 | EMSA with recombinant protein and EMSA supershift with DLD-1 NE | |
SRp20 | GUUGGACAAAUGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC | -10 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 19602482 | Goncalves V, Matos P, Jordan P. (2009) Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 18(19):3696-3707. | Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4. | In vivo splicing in HT29 | EMSA with recombinant protein and EMSA supershift with DLD-1 NE | |
SRp20 | GUUGGAGAAACGUACGGCAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC | -1 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 19602482 | Goncalves V, Matos P, Jordan P. (2009) Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways. Hum Mol Genet. 18(19):3696-3707. | Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4. | In vivo splicing in HT29 | EMSA with recombinant protein and EMSA supershift with DLD-1 NE | |
SRp20 | CCUCUUCC | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | CUUCAAC | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | UCUUCAC | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | CAUCAU | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | CUUCAC | 7 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | UCAUC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | UUCAC | 5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | CAUC | 3 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | GAUC | 1 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 17036044 | Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006) Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8. EMBO J. 25(21):5126-5137. | Synthesized sequences | NMR spectroscopy | ||
SRp20 | UUCUUCAUCC | -5 | Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3. The shuttling protein SRp20 binds TAP and can function as export factors (18364396). | 24321384 | Jang HN, Lee M, Loh TJ, Choi SW, Oh HK, Moon H, Cho S, Hong SE, Kim do H, Sheng Z, Green MR, Park D, Zheng X, Shen H. (2014) Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRSF3 through interaction with exon 8. Biochim Biophys Acta. 1839(1):25-32. | Construct of CASP2 [835] EX8 - INT8 - EX9 - INT9 - EX10 | In vivo splicing in MDA-MB-231 and HeLa | In vivo splicing with deletion and mutation analysis. RNA pulldown assay in HeLa nuclear extracts. | |
SRp30c | AAGAC | 1 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | ACCAC | 3 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGAAC | 1 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGCAC | 2 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGCAG | 5 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGGAA | 2 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGGAC | 10 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGGAG | 4 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGGAU | 1 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | AGGCA | 3 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | CGGAC | 1 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | CGGAG | 1 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | CGGUG | 1 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | UGGAC | 2 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | UGGAU | 2 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 17548433 | Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007) hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c. RNA 13: 1287-1300. | Synthesized oligos. Sequences of 20nt random for SELEX. | In vitro splicing with HeLa nuclear extracts, siRNA. | SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts. | |
SRp30c | CUGGAUU | -5 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 12024014 | Simard MJ, Chabot B. (2002) SRp30c is a repressor of 3' splice site utilization. Mol Cell Biol. 22(12): 4001-4010. | In this context, SRp30c has splicing repressor activity. | Construct of Adenovirus Major late EX_L1 - INT - EX_L2 with 3 copies of wt ISE or mutants inserted into the intron. | In vitro splicing with HeLa nuclear extract. | RNA affinity chromatography, mutation analysis and EMSA with HeLa nuclear extract. |
SRp30c | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | 5 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
SRp30c | UGAGUCGGAUCGCAGCUUGGAUGGCCAC | 5 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 18534987 | Cloutier P, Toutant J, Shkreta L, Goekjian S, Revil T, Chabot B. (2008) Antagonistic effects of the SRp30c protein and cryptic 5' splice sites on the alternative splicing of the apoptotic regulator Bcl-x. J Biol Chem. 283(31):21315-21324. | Construct of BCL2L1 [600039] EX1-EX2-INT2-EX3 | In vitro splicing assays in HeLa nuclear extracts and recombinant protein | EMSA using recombinant protein. UV cross-linking in HeLa and immunoprecipitation. | |
SRp30c | AGAUGGGCAAGGAGGUGGA | 5 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 16249178 | Park E, Han J, Son GH, Lee MS, Chung S, Park SH, Park K, Lee KH, Choi S, Seong JY, Kim K. (2006) Cooperative actions of Tra2alpha with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript. J Biol Chem. 281(1):401-409. | Sequences deriving from mouse Gnrh1 [14714]. Construct of Gnrh1 [14714] EX1 - INT1 - EX2 - EX3 - EX4. | In vitro splicing in HeLa NE | EMSA, UV cross-linking with recombinant protein | |
SRp30c | GAAAGUCUGAUUGAAGAGGAAGCCAGGCAGAAGAAGA | 5 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 16249178 | Park E, Han J, Son GH, Lee MS, Chung S, Park SH, Park K, Lee KH, Choi S, Seong JY, Kim K. (2006) Cooperative actions of Tra2alpha with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript. J Biol Chem. 281(1):401-409. | Sequences deriving from mouse Gnrh1 [14714]. Construct of Gnrh1 [14714] EX1 - INT1 - EX2 - EX3 - EX4. | In vitro splicing in HeLa NE | EMSA, UV cross-linking with recombinant protein | |
SRp30c | CCAGACACCGGAAACCCCUGCCACACCAC | 4 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp30c | GACGACGAGGAGCAGCAG | 8 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 11454855 | Tian H, Kole R. (2001) Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein. J Biol Chem. 276(36):33833-33839. | Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2). | In vitro splicing with HeLa NE | Filter binding assay, UV cross-linking and immunoprecipitation with HeLa NE | |
SRp30c | GACGACUCAGCAG | 4 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 11454855 | Tian H, Kole R. (2001) Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein. J Biol Chem. 276(36):33833-33839. | Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2). | In vitro splicing with HeLa NE | Filter binding assay, UV cross-linking and immunoprecipitation with HeLa NE | |
SRp30c | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | 2 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
SRp30c | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | 2 | Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
SRp38 | GACAAA | 4 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 18794844 | Feng Y, Chen M, Manley JL. (2008) Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator. Nat Struct Mol Biol. 15(10):1040-1048. | Constructs of beta-globin [3043]. | In vitro splicing with HeLa S100 extracts. Splicing-inhibition and spliceosome-assembly assays. | EMSA and RNase protection assay with recombinant protein. | |
SRp38 | AGACAA | 7 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 18794844 | Feng Y, Chen M, Manley JL. (2008) Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator. Nat Struct Mol Biol. 15(10):1040-1048. | Constructs of beta-globin [3043]. | In vitro splicing with HeLa S100 extracts. Splicing-inhibition and spliceosome-assembly assays. | EMSA and RNase protection assay with recombinant protein. | |
SRp38 | AAAGACAAA | 7 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 18794844 | Feng Y, Chen M, Manley JL. (2008) Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator. Nat Struct Mol Biol. 15(10):1040-1048. | Constructs of beta-globin [3043]. | In vitro splicing with HeLa S100 extracts. Splicing-inhibition and spliceosome-assembly assays. | EMSA and RNase protection assay with recombinant protein. | |
SRp38 | ACAAAGACAAA | 5 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 12419250 | Shin C, Manley JL. (2002) The SR protein SRp38 represses splicing in M phase cells. Cell. 111(3):407-417. | Sequences of 20 nt random for SELEX. | SELEX of random 20nt with recombinant protein with mitotic phase HeLa extracts, Western Blot. | ||
SRp38 | AAAGAAA | 5 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SRp38 | GAAAGAA | 5 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SRp38 | AAAGAAG | 5 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SRp38 | AAAAGAA | 5 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SRp38 | AGAGAAA | 5 | Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40. Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250) SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844). | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
SRp40 | AAAGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACACC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACACG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACAGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACAGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACCGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACGGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACGGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACUGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACUGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | AGAGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GCAGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GCAGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GCUGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UCCGC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UCGGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UCUGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CCGGAGGGGUCGGCUCGU | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UAGGAGGCUGGAUCGU | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UGAGAAGAUAGCACCA | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UGGGAGCAGUCGGCUCGA | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UGGGAGCUGAGCUCGC | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UUGGAGUAACAAGGAGUA | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UGGGAGCAGUCGGCUCAU | 3 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | CCGGAGGGGUUAGUUCCC | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UGGGAGCAAAGCUCGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | UGGGAGCAGUCGGCUCGU | 10 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9037021 | Tacke R, Chen Y, Manley JL. (1997) Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 94(4): 1148-1153. | Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX. | In vitro splicing in HeLa cell nuclear extracts. | SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts. | |
SRp40 | GAGGAAGAA | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 7651409 | Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995) A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer. Mol Cell Biol. 15(9):4898-4907. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assay, immunoprecipitation | |
SRp40 | GAAGAAGA | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 14729981 | Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004) RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon. Mol Cell Biol. 24(3):1387-1400. | Sequences of fibronectin FN1 [2335] EX_EDA and mutants | UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts | ||
SRp40 | AACACGGCUGUGAGUGGUCC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACAUGAACACAACGUCGGGG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACCAGGGUCGUCCGUCUGGG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACGCUCAAUAGAAAUCAAG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | ACGGGCGGACUCCUCUGGUA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | AGACCAGUAGCCGCUGCCGG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | AUGGGUCUGACACGCUGACU | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CAGGGCACUUGUUUCACUGG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CCAAUCGGAUCACCUAACGGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CCUCACUGGACUCAGUGGUG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CGACGUGUGGGGACGGCAAG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CGAGGAAUAUAAAGGUGGGA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | CUUGAGGUGAAGGUCAUGUG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GAAAGUUGUAAAGACAGGGG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GAACCUUGCAGGUCGCGCGA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GACGUUGGUGUUAUCCGCCA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GCAGUGACUGCAUUGGCAGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GGCGUUUUCGAGGAUCGGGA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GGUAAGUACUACAGGGUGUG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GUAGGACUGGAUCGCGUUGG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GUAUUUCGACACCAGUGUGA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GUGAUACAUACAGGUGGCGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UAGUAACCGCGACAGUAGGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UCUAAGGCGCUAAGAACGGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UGCAGAGGAUAGCCGGAACG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UGCUCACCCGGCCGCCACAGC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UGGAUGUCAGCGACGGGCCA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UGUGCAGCUUGCGUCACGUC | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | UUACAACUGCACCACGGUCG | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp40 | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
SRp40 | GGAAGAAGAUAAAGAC | 3 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 12826680 | Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003) The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20. J Biol Chem. 278(35):32943-32953. | Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10. | In vivo splicing in SVK14 and HEK293-EBNA cells. | UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts. | |
SRp40 | AUAAAGG | -5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 19843576 | Chandradas S, Deikus G, Tardos JG, Bogdanov VY. (2010) Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells. J Leukoc Biol. 87(1):147-152. | In this context, SRp40 and SC35 antagonize other SR proteins by competing for certain sites in exon 5, thereby promoting TF (tissue factor) exon 5 exclusion. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. |
SRp40 | CUGAUUUGUACCUAUUAGAUUC | 2 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp40 | UACUGAAGAACAAGUAUUU | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp40 | GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 16000324 | Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005) Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon. Hum Mol Genet. 14(16):2289-22303. | Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114]. | In vivo splicing in HEK293 cells. | Pulldown assay and western blot with HeLa NE | |
SRp40 | GGAGAGAAAGAAGGGAAGAUGAGAGG | 5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 16254078 | Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005) Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene. Nucleic Acids Res. 33(18):6000-6010. | Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3 | In vivo splicing in Hep3B | Pull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA. | |
SRp40 | GGUAUUUUUGGAGAAAUUC | -8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 17576688 | Buratti E, Stuani C, De Prato G, Baralle FE. (2007) SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer. Nucleic Acids Res. 35(13):4359-4368. | Sequences deriving from CFTR [1080]. Constructs of CFTR [1080] INT8 - EX9 - INT9. | In vivo splicing in Hep3B | UV cross-linking, immunoprecipitation with HeLa NE | |
SRp40 | UGUAUAAUAGAAAUUGUUC | -5 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 17576688 | Buratti E, Stuani C, De Prato G, Baralle FE. (2007) SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer. Nucleic Acids Res. 35(13):4359-4368. | Sequences deriving from CFTR [1080]. Constructs of CFTR [1080] INT8 - EX9 - INT9. | In vivo splicing in Hep3B | UV cross-linking, immunoprecipitation with HeLa NE | |
SRp40 | GGCAGGAAGAAGAGGAGCA | 8 | Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp54 | AAGAAG | -5 | Gene Name and Synonymous: SFRS11, splicing factor, arginine/serine-rich 11, p54. | 16943417 | Wu JY, Kar A, Kuo D, Yu B, Havlioglu N. (2006) SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy. Mol Cell Biol. 26(18):6739-6747. | Construct of Tau MAPT [4137] EX9 - INT9 - EX10 - INT10 - EX11 with GFP cDNA inserted into EX11. | In vivo splicing in HEK293. | Positive clones identified by fluorescence-activated cell sorting and visual inspection. Confirmed by UV crosslink, immunoprecipitation, SDS-PAGE. | |
SRp55 | ACCGGG | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | ACCGUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | AGCGGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | AUCGUA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CACGGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CCCGGC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGCGUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GACGUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GCCGGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GCCGUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UACGUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UCCGGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UGCGGC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UGCGUA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UGCGUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UGCGUG | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GAGGAAGAA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 7651409 | Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995) A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer. Mol Cell Biol. 15(9):4898-4907. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assay, immunoprecipitation | |
SRp55 | AAGAGGAAGAAUGGCUUGAGGAAGACGACG | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9436904 | Nagel RJ, Lancaster AM, Zahler AM. (1998) Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55. RNA. 4(1):11-23. | Sequences of Gallus gallus cardiac troponin T TNNT2 [396433] EX5 and mutants for EMSA. Construct of beta-globin [3043] EX1 - INT1 - EX2 for in vitro splicing. | In vitro splicing with HeLa S100 extracts, also in competition with EX5 and mutants of TNNT2. | EMSA with purified protein. Hill plot analysis. Chemical modification interference with kethoxal. | |
SRp55 | GCAGCACCUGGC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 12549914 | Tran Q, Roesser JR. (2003) SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing. Biochemistry. 42(4):951-957. | Construct of calcitonin/calcitonin gene-related peptide CALCA [796] EX3 - INT3 - EX4 - INT4 - EX5. | In vitro splicing with HeLa and HEK 293 cells nuclear extracts. | EMSA, UV crosslink. | |
SRp55 | GAAGAAGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 14729981 | Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004) RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon. Mol Cell Biol. 24(3):1387-1400. | Sequences of fibronectin FN1 [2335] EX_EDA and mutants | UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts | ||
SRp55 | AGACCGUCAACAUGUCUGCC | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | AUAGCGAGCGGAAAACAGGUAA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | AUGCAGACGAUGGUGCGGCU | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CAAACCGUCAAAGUACGUCA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CAAACCUGCGUGGUAUGGUA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CACCAGCGGAGUCCCCAGAGC | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CAUGAAGCCGUCACCAACGUCUAG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGAGCCACGGACCACACGGA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGAUUCAGGUACGUCCAACU | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGCACACUGCGUCCCGGGGC | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGCGUCGUGUCGUAGGGGGC | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGCGUGCGUGCAGUGCCAAG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | CGUGUCGCGUCCUCGUGUGC | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GACCGGGAUUGAAGGAGCU | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GACGUCGCCCCGUGUGUAAG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GAGUUGAGCGAUGGUGCGUA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GAUCGAAUCCGGAACACAGG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GAUCGUAUCCGGAACACGGG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GGAUAACGGUGUGGCCCGGC | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GGAUCGAAUCCGGAACACGG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GGAUCGUAAGUGCAGACGA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GGAUCGUAAGUGCAGAUGA | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GGCCGGACGCAUUGCAGAG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | UCCGAUCUGUGCACGGACG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9649504 | Liu HX, Zhang M, Krainer AR. (1998) Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 12(13): 1998-2012. | Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random. | SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts. | UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts. | |
SRp55 | GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 18597733 | Baralle M, Pastor T, Bussani E, Pagani F. (2008) Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing. Am J Hum Genet. 83(1): 77-88. | Synthesized sequences. | Mass spectrometry, immunoblot analysis. | ||
SRp55 | AAGGGAGGGAAUGGCUUGAGGAAGACGACG | 2 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9436904 | Nagel RJ, Lancaster AM, Zahler AM. (1998) Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55. RNA. 4(1):11-23. | Sequences of Gallus gallus cardiac troponin T TNNT2 [396433] EX5 and mutants for EMSA. Construct of beta-globin [3043] EX1 - INT1 - EX2 for in vitro splicing. | In vitro splicing with HeLa S100 extracts, also in competition with EX5 and mutants of TNNT2. | EMSA with purified protein. Hill plot analysis. Chemical modification interference with kethoxal. | |
SRp55 | AAGAGGAAGAAGAAGAAGAGGAAGACGACG | 8 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 9436904 | Nagel RJ, Lancaster AM, Zahler AM. (1998) Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55. RNA. 4(1):11-23. | Sequences of Gallus gallus cardiac troponin T TNNT2 [396433] EX5 and mutants for EMSA. Construct of beta-globin [3043] EX1 - INT1 - EX2 for in vitro splicing. | In vitro splicing with HeLa S100 extracts, also in competition with EX5 and mutants of TNNT2. | EMSA with purified protein. Hill plot analysis. Chemical modification interference with kethoxal. | |
SRp55 | GAUCAACCUGGC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 12549914 | Tran Q, Roesser JR. (2003) SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing. Biochemistry. 42(4):951-957. | Construct of calcitonin/calcitonin gene-related peptide CALCA [796] EX3 - INT3 - EX4 - INT4 - EX5. | In vitro splicing with HeLa and HEK 293 cells nuclear extracts. | EMSA, UV crosslink. | |
SRp55 | GCUCAUCCUGGC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 12549914 | Tran Q, Roesser JR. (2003) SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing. Biochemistry. 42(4):951-957. | Construct of calcitonin/calcitonin gene-related peptide CALCA [796] EX3 - INT3 - EX4 - INT4 - EX5. | In vitro splicing with HeLa and HEK 293 cells nuclear extracts. | EMSA, UV crosslink. | |
SRp55 | UGUGGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 18315555 | Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008) SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. J Thromb Haemost. 6(5):877-884. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 and SC cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. | |
SRp55 | UGCGGA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 18315555 | Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008) SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. J Thromb Haemost. 6(5):877-884. | Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutants | In vivo splicing in THP-1 and SC cells. | Mutagenesis, splicing assays, EMSA, Immunoblot. | |
SRp55 | UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | CUGAUUUGUAUUUAUUAGACUC | 1 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | AACAGAAAAAGAAAUAUUU | 2 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | CUGAUUUGUACCUAUUAGAUUC | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | UACUGAAGAACAAGUAUUU | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp55 | GGAGAGAAAGAAGGGAAGAUGAGAGG | 5 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 16254078 | Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005) Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene. Nucleic Acids Res. 33(18):6000-6010. | Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3 | In vivo splicing in Hep3B | Pull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA. | |
SRp55 | GGCAGGAAGAAGAGGAGCA | 3 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp55 | CCAGACACCGGAAACCCCUGCCACACCAC | 2 | Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp75 | GAGGAAGAA | 5 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 7651409 | Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995) A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer. Mol Cell Biol. 15(9):4898-4907. | Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 | In vitro splicing in HeLa nuclear extracts. | UV crosslink, competition assay, immunoprecipitation | |
SRp75 | GAAGAAGA | 5 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 14729981 | Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004) RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon. Mol Cell Biol. 24(3):1387-1400. | Sequences of fibronectin FN1 [2335] EX_EDA and mutants | UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts | ||
SRp75 | GGACAGCAGAGAUCCAGU | 5 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 18272582 | Exline CM, Feng Z, Stoltzfus CM. (2008) Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression. J Virol. 82(8): 3921-3931. | Constructs of Drosophila dsx [40940] EX3-INT3-20bpEX4 adding wt or mutated ESE at EX4. | In vitro-splicing with HeLa nuclear extract. | UV cross-linking and immunoprecipitation in HeLa nuclear extract. | |
SRp75 | CUGAUUUGUACCUAUUAGAUUC | 2 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp75 | UACUGAAGAACAAGUAUUU | 2 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 19910374 | Haque A, Buratti E, Baralle FE. (2010) Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12. Nucleic Acids Res. 38(2):647-659. | Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences. | In vivo splicing assay in HeLa cells. | Mutagenesis, western blot, siRNA knockdown | |
SRp75 | UGUGUCACUGUCUGGUUC | 3 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp75 | GGCAGGAAGAAGAGGAGCA | 8 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
SRp75 | CCAGACACCGGAAACCCCUGCCACACCAC | 2 | Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
TDP43 | UGUGUGUGUG | -3 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 15826655 | Ayala YM, Pantano S, D'Ambrogio A, Buratti E, Brindisi A, Marchetti C, Romano M, Baralle FE. (2005) Human, Drosophila, and C.elegans TDP43: nucleic acid binding properties and splicing regulatory function. J Mol Biol. 348(3):575-588. | Construct of tropomyosin 1 alpha TPM1 [7168] EX2 and EX3 separated by a synthetic intron with the 5' end partially corresponding to beta-globin [3043]. | In vitro splicing with HeLa nuclear extracts. | UV crosslinking, EMSA. | |
TDP43 | UGUGUGUGUGUG | -4 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 11470789 | Buratti E, Baralle FE. (2001) Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9. J Biol Chem. 276(39):36337-36343. | synthesized oligos | UV crosslink, SDS-PAGE, EMSA with recombinant protein. | ||
TDP43 | UGUGUGUGUGUGUGUGUG | -6 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 11470789 | Buratti E, Baralle FE. (2001) Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9. J Biol Chem. 276(39):36337-36343. | synthesized oligos | UV crosslink, SDS-PAGE, EMSA with recombinant protein. | ||
TDP43 | UGUGUGUGUGUGUGUGUGUGUG | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 15826655 | Ayala YM, Pantano S, D'Ambrogio A, Buratti E, Brindisi A, Marchetti C, Romano M, Baralle FE. (2005) Human, Drosophila, and C.elegans TDP43: nucleic acid binding properties and splicing regulatory function. J Mol Biol. 348(3):575-588. | Construct of tropomyosin 1 alpha TPM1 [7168] EX2 and EX3 separated by a synthetic intron with the 5' end partially corresponding to beta-globin [3043]. | In vitro splicing with HeLa nuclear extracts. | UV crosslinking, EMSA. | |
TDP43 | UGUGUGUGUGUGUGUGUGUGUGUGUGUUU | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 16458894 | Ayala YM, Pagani F, Baralle FE. (2006) TDP43 depletion rescues aberrant CFTR exon 9 skipping. FEBS Lett. 580(5):1339-1344. | Constructs of CFTR [1080] EX9 along with part of the flanking introns. | In vivo splicing in HeLa, Hep3B, lymphoblasts and EBV transformed lymphocytes. | Western blot, siRNA. | |
TDP43 | UGUGUG | -2 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 11470789 | Buratti E, Baralle FE. (2001) Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9. J Biol Chem. 276(39):36337-36343. | synthesized oligos | UV crosslink, SDS-PAGE, EMSA with recombinant protein. | ||
TDP43 | UGUGUGUG | -2 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 15826655 | Ayala YM, Pantano S, D'Ambrogio A, Buratti E, Brindisi A, Marchetti C, Romano M, Baralle FE. (2005) Human, Drosophila, and C.elegans TDP43: nucleic acid binding properties and splicing regulatory function. J Mol Biol. 348(3):575-588. | Construct of tropomyosin 1 alpha TPM1 [7168] EX2 and EX3 separated by a synthetic intron with the 5' end partially corresponding to beta-globin [3043]. | In vitro splicing with HeLa nuclear extracts. | UV crosslinking, EMSA. | |
TDP43 | GUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGU | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 16254078 | Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005) Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene. Nucleic Acids Res. 33(18):6000-6010. | Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3 | In vivo splicing in Hep3B | Pull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA. | |
TDP43 | UGUGUGUGUGUGUGUGUGUGUGUUUUU | -1 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
TDP43 | UGUGUGUGUGUGUGUGUGUGUGUGUUUUU | -2 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
TDP43 | UGUGUGUGUGUGUGUGUGUGUGUGUGUUUUU | -3 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 20631008 | Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010) CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3. Nucleic Acids Res. 38(20):7273-7285. | Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9 | In vivo splicing in HeLa, HGT-1, HCT116 | EMSA with recombinant protein. siRNA. | |
TDP43 | UGAGGUAGUAGGUUGUGUGGUU | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 20423455 | Buratti E, De Conti L, Stuani C, Romano M, Baralle M, Baralle F. (2010) Nuclear factor TDP-43 can affect selected microRNA levels. FEBS J. 277(10):2268-2281. | Sequences deriving from MIRLET7B [406884] and mutated MIRLET7A1 [406881], mutated MIRLET7A2 [406882], mutated MIRLET7A3 [406883]. | EMSA with recombinant protein | ||
TDP43 | UGAGGUAGUAGGUUGUGUAGUU | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 20423455 | Buratti E, De Conti L, Stuani C, Romano M, Baralle M, Baralle F. (2010) Nuclear factor TDP-43 can affect selected microRNA levels. FEBS J. 277(10):2268-2281. | Sequences deriving from MIRLET7B [406884] and mutated MIRLET7A1 [406881], mutated MIRLET7A2 [406882], mutated MIRLET7A3 [406883]. | EMSA with recombinant protein | ||
TDP43 | GUUGUGC | -10 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 19815002 | Volkening K, Leystra-Lantz C, Yang W, Jaffee H, Strong MJ. (2009) Tar DNA binding protein of 43 kDa (TDP-43), 14-3-3 proteins and copper/zinc superoxide dismutase (SOD1) interact to modulate NFL mRNA stability. Implications for altered RNA processing in amyotrophic lateral sclerosis (ALS). Brain Res. 1305:168-182. | Sequences deriving fromwt and mutated NEFL [4747] | Immunoprecipitation in HEK293 cells. | ||
TDP43 | GUUUUGC | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 19815002 | Volkening K, Leystra-Lantz C, Yang W, Jaffee H, Strong MJ. (2009) Tar DNA binding protein of 43 kDa (TDP-43), 14-3-3 proteins and copper/zinc superoxide dismutase (SOD1) interact to modulate NFL mRNA stability. Implications for altered RNA processing in amyotrophic lateral sclerosis (ALS). Brain Res. 1305:168-182. | Sequences deriving fromwt and mutated NEFL [4747] | Immunoprecipitation in HEK293 cells. | ||
TDP43 | GUUGUUC | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 19815002 | Volkening K, Leystra-Lantz C, Yang W, Jaffee H, Strong MJ. (2009) Tar DNA binding protein of 43 kDa (TDP-43), 14-3-3 proteins and copper/zinc superoxide dismutase (SOD1) interact to modulate NFL mRNA stability. Implications for altered RNA processing in amyotrophic lateral sclerosis (ALS). Brain Res. 1305:168-182. | Sequences deriving fromwt and mutated NEFL [4747] | Immunoprecipitation in HEK293 cells. | ||
TDP43 | UGUGUGUGUGUGUGUGUGUGUGUG | -5 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 16157593 | Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005) TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing. J Biol Chem. 280(45):37572-37584. | Synthesized sequences | EMSA with recombinant protein | ||
TDP43 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | -2 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
TDP43 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | -2 | Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10. It can act as transcriptional repressor (21252238) | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
TIA-1 | UUUUUUAUUUU | -5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17035636 | Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006) A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators. Mol Biol Cell. 17(12):5105-5114. | Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus | UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts. | ||
TIA-1 | UUUUUUUUUUUAGUUGCUAAUUUAUUGUUUU | 8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 11106748 | Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J. (2000) The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing. Mol Cell. 6(5): 1089-1098. | In this context, TIA-1 promotes exon 6 inclusion. | Synthesized sequences. Construct of Drosophila msl-2 [33565] INT1 and flanking sequences. Construct of human FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. | In vitro splicing of msl-2 construct and reconstitution assay. In vivo splicing of FAS construct in mouse fibroblast Bk6 cell line overexpressing human TIA-1 | UV-crosslink, Filter binding assay, Mutational analysis, Immunoprecipitation with HeLa nuclear extract. |
TIA-1 | UUCAUUUUUGAUUCUUUGGUUAAAAAA | -5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 19339348 | Choi EY, Pintel D. (2009) Splicing of the large intron present in the non-structural gene of minute virus of mice (MVM) is governed by TIA-1/TIAR binding downstream of the non-consensus donor. J Virol. 83(12):6306-6311 | Synthesized sequences. | RNA chromatography affinity and immunoblotting in HeLa nuclear extract. | ||
TIA-1 | UUAUUUUUUUACGGUUAUAUUCUCCUUUCCC | 5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 11106748 | Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J. (2000) The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing. Mol Cell. 6(5): 1089-1098. | In this context, TIA-1 promotes exon 6 inclusion. | Synthesized sequences. Construct of Drosophila msl-2 [33565] INT1 and flanking sequences. Construct of human FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. | In vitro splicing of msl-2 construct and reconstitution assay. In vivo splicing of FAS construct in mouse fibroblast Bk6 cell line overexpressing human TIA-1 | UV-crosslink, Filter binding assay, Mutational analysis, Immunoprecipitation with HeLa nuclear extract. |
TIA-1 | UGUGUGUGUGUAGUUGCUAAUUUAUUGUUUU | 4 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 11106748 | Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J. (2000) The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing. Mol Cell. 6(5): 1089-1098. | In this context, TIA-1 promotes exon 6 inclusion. | Synthesized sequences. Construct of Drosophila msl-2 [33565] INT1 and flanking sequences. Construct of human FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. | In vitro splicing of msl-2 construct and reconstitution assay. In vivo splicing of FAS construct in mouse fibroblast Bk6 cell line overexpressing human TIA-1 | UV-crosslink, Filter binding assay, Mutational analysis, Immunoprecipitation with HeLa nuclear extract. |
TIA-1 | AUUUA | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | AUUUC | 4 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | AUUUUC | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | AUUUUG | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUA | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUC | 10 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUG | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUUA | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUUC | 5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUUG | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUUUA | 4 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | CUUUUUC | 3 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUA | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUC | 5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUG | 4 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUUA | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUUC | 2 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUUG | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUUUC | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUUUG | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | GUUUUUUUUC | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIA-1 | UCUUGCUUUGUUCAAAC | 5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 16109372 | Izquierdo JM, Majos N, Bonnal S, Martinez C, Castelo R, Guigo R, Bilbao D, Valcarcel J. (2005) Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition. Mol Cell. 19(4):475-484. | Sequences deriving from wt and mutated FAS [355]. Construct of FAS [355] EX5 - INT5 - EX6 - INT6 - EX7 | In vitro splicing in HeLa NE | UV cross-linking, immunoprecipitation with HeLa NE | |
TIA-1 | UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG | -5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 16890199 | Izquierdo JM. (2006) Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR. Biochem Biophys Res Commun. 348(2):703-711. | Sequences deriving from ATP5B [506] | UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein. | ||
TIA-1 | UAUUGCUUGGGUAAUUUUUUUUUUUAGUUGCUAAUUUAUUG | 8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17488725 | Izquierdo JM, Valcarcel J. (2007) Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein. J Biol Chem. 282(27):19410-19417. | Sequences deriving from FAS [355] and Drosophila msl-2 [33565] | EMSA with recombinant protein | ||
TIA-1 | GCCAAUUCCACUAAUUGUUUGGGGUAAGUUCUUGCUUUGUUCAAACUGCAGAUUGAAA | 4 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17488725 | Izquierdo JM, Valcarcel J. (2007) Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein. J Biol Chem. 282(27):19410-19417. | Sequences deriving from FAS [355] and Drosophila msl-2 [33565] | EMSA with recombinant protein | ||
TIA-1 | GUAAUUUAUUUAUUUCCUGUUCAACAUAAAUAAAUUAC | 5 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17580305 | McAlinden A, Liang L, Mukudai Y, Imamura T, Sandell LJ. (2007) Nuclear protein TIA-1 regulates COL2A1 alternative splicing and interacts with precursor mRNA and genomic DNA. J Biol Chem. 282(33):24444-24454. | Sequences deriving from COL2A1 [1280]. Constructs of COL2A1 [1280] EX1 - INT1 - EX2 - INT2 - EX3. | In vivo splicing in TC28 | UV cross-linking with recombinant protein | |
TIA-1 | UUAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUU | -8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
TIA-1 | UUUUUAAAUAUUUAUUUAUUUAUUUAUUUAUUUU | -8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
TIA-1 | GUUUUUAAUUUAUUUAUUAAGAUGGAUUCU | -8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
TIA-1 | AAACCUAUUUAUUAAUAUUUAAAACUAUUUAUAUG | -8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
TIA-1 | CUAAUGAUCAUAUUUAUUUAUUUAUAUGAACCAUG | -8 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 17965020 | Buxade M, Morrice N, Krebs DL, Proud CG. (2008) The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha. J Biol Chem. 283(1):57-65. | Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. | Pull-down, western blot with Jurkat extracts. | ||
TIA-1 | GUUAUAUUCUCCUUUC | 10 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 24682828 | Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. (2014) Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res. | Sequences deriving from FAS [355]. Synthetic sequences. | NMR, isothermal titration calorimetry and SAXS. | ||
TIA-1 | UUCUCCUUUC | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 24682828 | Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. (2014) Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res. | Sequences deriving from FAS [355]. Synthetic sequences. | NMR, isothermal titration calorimetry and SAXS. | ||
TIA-1 | UUUUUUUUU | 1 | Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. | 24682828 | Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. (2014) Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res. | Sequences deriving from FAS [355]. Synthetic sequences. | NMR, isothermal titration calorimetry and SAXS. | ||
TIAL1 | UUUUUUAUUUU | -5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 17035636 | Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006) A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators. Mol Biol Cell. 17(12):5105-5114. | Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus | UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts. | ||
TIAL1 | UUCAUUUUUGAUUCUUUGGUUAAAAAA | -5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 19339348 | Choi EY, Pintel D. (2009) Splicing of the large intron present in the non-structural gene of minute virus of mice (MVM) is governed by TIA-1/TIAR binding downstream of the non-consensus donor. J Virol. 83(12):6306-6311 | Synthesized sequences. | RNA chromatography affinity and immunoblotting in HeLa nuclear extract. | ||
TIAL1 | AUUUA | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUC | 7 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUUA | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUUC | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUUG | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUUUA | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUUUUUC | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | AUUUUUUUUUUUA | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUA | 3 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUC | 10 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUG | 5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUA | 5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUC | 5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUG | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUUA | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUUC | 3 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUUG | 5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUUUC | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | CUUUUUUUUUUUC | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUC | 6 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUG | 5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUUC | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUUUC | 2 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUUUG | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUUUUUC | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | GUUUUUUUUG | 1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 8576255 | Dember LM, Kim ND, Liu KQ, Anderson P. (1996) Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities. J Biol Chem. 271(5):2783-2788. | Sequences of 68 nt random for SELEX. | SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay. | ||
TIAL1 | UUUUAAAUUUU | -5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 12356730 | Iseni F, Garcin D, Nishio M, Kedersha N, Anderson P, Kolakofsky D. (2002) Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis. EMBO J. 21(19):5141-5150. | Sendai virus (SeV) trailer (tr) RNA and mutants. | UV cross-linking and immunoprecipitation with HeLa cytoplasmic extracts | ||
TIAL1 | UUUUCAGUUUU | -5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 12356730 | Iseni F, Garcin D, Nishio M, Kedersha N, Anderson P, Kolakofsky D. (2002) Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis. EMBO J. 21(19):5141-5150. | Sendai virus (SeV) trailer (tr) RNA and mutants. | UV cross-linking and immunoprecipitation with HeLa cytoplasmic extracts | ||
TIAL1 | AAAAUGCUUUUUUUUUUUUA | -3 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 16091628 | Suswam EA, Li YY, Mahtani H, King PH. (2005) Novel DNA-binding properties of the RNA-binding protein TIAR. Nucleic Acids Res. 33(14):4507-4518. | Sequences deriving from VEGFA [7422] | UV cross-linking, competition assay with recombinant protein | ||
TIAL1 | UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG | -5 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 16890199 | Izquierdo JM. (2006) Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR. Biochem Biophys Res Commun. 348(2):703-711. | Sequences deriving from ATP5B [506] | UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein. | ||
TIAL1 | UUGCCACCUCCUGCUCCUGCCCAGACAG | -10 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 17682065 | Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA. (2007) Elucidation of a C-rich signature motif in target mRNAs of RNA-binding protein TIAR. Mol Cell Biol. 27(19):6806-6817. | Synthesized sequences | Surface plasmon resonance | ||
TIAL1 | GGGGGGUUUUUUUUUUUUUUUUUGGGGG | -1 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 17682065 | Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA. (2007) Elucidation of a C-rich signature motif in target mRNAs of RNA-binding protein TIAR. Mol Cell Biol. 27(19):6806-6817. | Synthesized sequences | Surface plasmon resonance | ||
TIAL1 | GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC | 6 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
TIAL1 | AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC | 4 | Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. | 23381195 | Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013) hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations. Acta Neuropathol. 125(3):413-423. | Synthetic sequences. | RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot. | ||
YB-1 | ACAAC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18503770 | Skoko N, Baralle M, Buratti E, Baralle FE. (2008) The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors. FEBS Lett. 582(15):2231-2236. | Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37. | In vivo splicing in HeLa, siRNA. | UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts. | |
YB-1 | CACCAGUCACCGC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 11447123 | Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001) The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4. EMBO J. 20(14):3821-3830. | synthesized oligos | UV crosslink and competition assay in HeLa nuclear extracts. | ||
YB-1 | CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 12447348 | Hui J, Stangl K, Lane WS, Bindereif A. (2003) HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. Nat Struct Biol. 10(1): 33-37. | In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. | Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences. | In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation. | UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis. |
YB-1 | CAACCACACCAC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 11447123 | Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001) The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4. EMBO J. 20(14):3821-3830. | synthesized oligos | UV crosslink and competition assay in HeLa nuclear extracts. | ||
YB-1 | ACCACACAAAACA | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 11447123 | Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001) The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4. EMBO J. 20(14):3821-3830. | synthesized oligos | UV crosslink and competition assay in HeLa nuclear extracts. | ||
YB-1 | CAACCACAA | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 11447123 | Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001) The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4. EMBO J. 20(14):3821-3830. | synthesized oligos | UV crosslink and competition assay in HeLa nuclear extracts. | ||
YB-1 | AGAAC | 2 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18503770 | Skoko N, Baralle M, Buratti E, Baralle FE. (2008) The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors. FEBS Lett. 582(15):2231-2236. | Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37. | In vivo splicing in HeLa, siRNA. | UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts. | |
YB-1 | CCUGCGG | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
YB-1 | GCCUGCG | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
YB-1 | CUGCGGU | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
YB-1 | GGUCUGC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
YB-1 | CCCUGCG | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 19561594 | Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins. Nat Biotechnol. 27(7):667-670. | Synthesized sequences | RNAcompete using recombinant protein | ||
YB-1 | GGUCUCUCUGGUUAGACCUGAUCUGAGCCUGGGAGCUCUCUGGCUAACUAGGGAACC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 10573156 | Ansari SA, Safak M, Gallia GL, Sawaya BE, Amini S, Khalili K. (1999) Interaction of YB-1 with human immunodeficiency virus type 1 Tat and TAR RNA modulates viral promoter activity. J Gen Virol. 80 (Pt 10):2629-2638. | Synthesized sequences and HIV-1 TAR | EMSA with recombinant protein | ||
YB-1 | GGCCUGAUCUGAGCCUGGGAGCUCUCUGGCC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 10573156 | Ansari SA, Safak M, Gallia GL, Sawaya BE, Amini S, Khalili K. (1999) Interaction of YB-1 with human immunodeficiency virus type 1 Tat and TAR RNA modulates viral promoter activity. J Gen Virol. 80 (Pt 10):2629-2638. | Synthesized sequences and HIV-1 TAR | EMSA with recombinant protein | ||
YB-1 | CCCUCUGCCACCCAGGCAGGCCCUGCCUUCAGCCCUGGCCCAGAGCUGGAACACUCUCUGAGAUGCCCCUCUGCCUGGGCUUAUGCCCUCAGAUGGAGACAUUGGAUGUGGAGCUCCUGCUGGAUGCGUGCCCUGACCCCUGCACCAGCCCUUCCCUGCUUUGAG | -5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 12600897 | Skalweit A, Doller A, Huth A, Kahne T, Persson PB, Thiele BJ. (2003) Posttranscriptional control of renin synthesis: identification of proteins interacting with renin mRNA 3'-untranslated region. Circ Res. 92(4):419-427. | Sequences deriving from REN [5972] | EMSA, UV cross-linking with Calu-6 cell extracts. RNA-affinity chromatography with MALDI-TOF-MS. Western blot. | ||
YB-1 | UCUCGGGGGGGUUUUCAUCUAUGAGGGUGUUUCCUCUAAACCUACGAGGGAGGAACACCUGAUCUUACAGAAAAUACCACCUCGAGA | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 16508950 | Shen Q, Fan L, Newburger PE. (2006) Nuclease sensitive element binding protein 1 associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation. J Cell Physiol. 207(3):775-783. | Sequences deriving from GPX1 [2876] | UV cross-linking, immunoprecipitation in HeLa | ||
YB-1 | GCAGCCAGACAGCGAGGGCC | -1 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18075498 | Fraser DJ, Phillips AO, Zhang X, van Roeyen CR, Muehlenberg P, En-Nia A, Mertens PR. (2008) Y-box protein-1 controls transforming growth factor-beta1 translation in proximal tubular cells. Kidney Int. 73(6):724-732. | Sequences deriving from TGFB1 [7040] | EMSA supershift, UV cross-linking, competition assay with HK-2 protein extracts. | ||
YB-1 | GGGCACCCCCCCGGCUCUG | -10 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18075498 | Fraser DJ, Phillips AO, Zhang X, van Roeyen CR, Muehlenberg P, En-Nia A, Mertens PR. (2008) Y-box protein-1 controls transforming growth factor-beta1 translation in proximal tubular cells. Kidney Int. 73(6):724-732. | Sequences deriving from TGFB1 [7040] | EMSA supershift, UV cross-linking, competition assay with HK-2 protein extracts. | ||
YB-1 | CUGCACCACCACCUAUCUA | 2 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
YB-1 | GGCAGGAAGAAGAGGAGCA | 8 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
YB-1 | CCAGACACCGGAAACCCCUGCCACACCAC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 18945760 | Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009) Control of the papillomavirus early-to-late switch by differentially expressed SRp20. J Virol. 83(1):167-180. | Sequences deriving from BPV-1 and HPV-16 mRNAs. | EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract. | ||
YB-1 | CAUC | 10 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
YB-1 | CACC | 6 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
YB-1 | GAUC | 5 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
YB-1 | CAUCUG | 10 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
YB-1 | CAUCGC | 8 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
YB-1 | GAUCUG | 8 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
YB-1 | CAUCAUC | 10 | Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. | 22730292 | Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012) YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. Nucleic Acids Res. 40(17):8622-8636. | Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV | In vivo splicing in HEK-293 cells. | SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking. | |
ZRANB2 | AGGUAA | -10 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | CGGUAA | -3 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | UGGUAA | -2 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGUAG | -2 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | CGGUCU | -2 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGUCU | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | CGGUAU | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGUAC | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGUAU | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGUUA | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGUUU | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | CGGUAG | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | GAGGUU | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | GGGUAA | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | GGGUGA | -1 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | AGGGAA | -5 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 19304800 | Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009) The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences. Proc Natl Acad Sci U S A. 106(14): 5581-5586. | Two close motifs make binding significantly stronger. | Synthesized oligos. Sequences of 25nt random for SELEX. | SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy. | |
ZRANB2 | GGUGGU | -10 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 22517726 | O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012) Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angew Chem Int Ed Engl. 51(22):5371-5375. | Synthesized sequences | Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein | ||
ZRANB2 | GGUAAAGGU | -10 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 22517726 | O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012) Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angew Chem Int Ed Engl. 51(22):5371-5375. | Synthesized sequences | Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein | ||
ZRANB2 | GGUGGUGGU | -10 | Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117. ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987). | 22517726 | O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012) Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA. Angew Chem Int Ed Engl. 51(22):5371-5375. | Synthesized sequences | Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein |