SpliceAid 2 splicing factor binding sites and related information
PROTEIN NAME
SITE
SCORE
PROTEIN NOTES
PMID REFERENCE
REFERENCE
ARTICLE NOTES
GENE/CONSTRUCT (Target RNA)
SPLICING ASSAY
BINDING ASSAY
9G8ACACGACGAU8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAAUGAU6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAGACUA4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAGAGAA8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAGAGAC10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAGAGAU10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAGAUCA4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAUAGAA6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAUAGAC8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAUUGAC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACGAUUGAU6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8ACUAGAGAC8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACAACGAU8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACAACGUU6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACCACGCU6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACGACGAA8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACGACGAU10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACGACGUU8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACAAG6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACACC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACAUA4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACAUC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACGAC10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACGAG8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACGAU10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACGCU8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUACGUU8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AGACUUCGAU6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AUACGACGAG6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8AUCCGACGAG4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CAACGACGAG4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CAGAGAGAC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CCGAGAUCA2Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CCGAGAUCU4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CGACGAGGAC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CGACGAUGAC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CUACGACGAG4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8CUGAGAGAC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8UAGAGAGAC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8UCGAGAGAU8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8UCGAUUGAC4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8UGACGACGAA6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8UGACGACGAU10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
9G8GGACGACGA5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
9G8AAAGGACAAA5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
9G8UCUUCA5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
9G8UCUCCG5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
9G8UGGACAA5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
9G8GGAAGAAGAUAAAGAC8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
12826680Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003)
The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20.
J Biol Chem. 278(35):32943-32953.
 Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10.In vivo splicing in SVK14 and HEK293-EBNA cells.UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts.
9G8GACGACGAG5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
10523623Bourgeois CF, Popielarz M, Hildwein G, Stevenin J. (1999)
Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation.
Mol Cell Biol. 19(11):7347-7356.
 Construct and variants of Adenovirus E1A unit.in vitro splicing with Hela nuclear extracts, in vivo splicing in Hela cells, in vitro complementation assays.UV crosslink, immunoprecipitation.
9G8AGAGGAAGGCGA7Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
9G8AGGAGCAGGGGACGAAG10Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
9G8GAAGAAGAA5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
9G8CUAGCUCACGCAGCAUAUCUCACGCAUCAUG2Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
9G8ACAACAAGAAGACGCGCAUCAU2Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11336712Huang Y, Steitz JA. (2001)
Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA.
Mol Cell. 7(4):899-905.
 Sequences wt and mutated deriving from mouse Histone 2A UV cross-linking and immunoprecipitation with HeLa NE
9G8GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
9G8UCAACA5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
8769651Lynch KW, Maniatis T. (1996)
Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer.
Genes Dev. 10(16):2089-2101.
 Sequences deriving from D. melanogaster dsx [40940]. UV-cross link, immunoprecipitation with HeLa extracts.
9G8GACGACGAGGAGCAGCAG8Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11454855Tian H, Kole R. (2001)
Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein.
J Biol Chem. 276(36):33833-33839.
 Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2).In vitro splicing with HeLa NEFilter binding assay, UV cross-linking and immunoprecipitation with HeLa NE
9G8GACGACUCAGCAG4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
11454855Tian H, Kole R. (2001)
Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein.
J Biol Chem. 276(36):33833-33839.
 Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2).In vitro splicing with HeLa NEFilter binding assay, UV cross-linking and immunoprecipitation with HeLa NE
9G8CUCUUCAC5Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
9G8GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC6Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
9G8AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC4Gene Name and Synonymous: SFRS7, splicing factor arginine/serine-rich 7 35kDa, AAG3, HSSG1, RBM37, ZCRB2, ZCCHC20
The shuttling protein 9G8 binds TAP and can function as export factors (18364396).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
CUG-BP1UGUGU-2Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUG-3Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUGU-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUGUG-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUGUGU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUGUGUG-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUGUGUGU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUUGUGUGU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUGUGUGUGUGUGU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1UGUUUGU-2Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
16938098Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB. (2006)
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
Biochem J. 400(2): 291-301.
 Sequences of 35nt random for SELEX. SELEX of 35nt random with recombinant protein. Surface plasmon resonance and EMSA.
CUG-BP1CUGCUGCUGCUGCUGCUGCUGCUG-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
8789448Timchenko LT, Timchenko NA, Caskey CT, Roberts R. (1996)
Novel proteins with binding specificity for DNA CTG repeats and RNA CUG repeats: implications for myotonic dystrophy.
Hum Mol Genet. 5(1):115-121.
CUG-BP1 has no affinity to (CGG)8 repeats. synthesized oligos EMSA with HeLa whole cell extract.
CUG-BP1UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUG-10Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCG-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1UCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUGUCUG-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGACAGA-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1UCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCGUCCG-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGA-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUG-2Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCG-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1UAUGUAUGUAUGUAUGUAUGUAUGUAUG-6Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CAUGCAUGCAUGCAUGCAUGCAUGCAUG-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG-2Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CCUGCCUGCCUGCCUGCCUGCCUGCCUG-2Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG-2Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
CUG-BP1CUGUCUG-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
12649496Gromak N, Matlin AJ, Cooper TA, Smith CW. (2003)
Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein.
RNA. 9(4):443-456.
 Construct of rat Actn1 [81634] EX_NM - INTIn vitro splicing with HeLa nuclear extracts.UV cross-link with recombinant protein and with HeLa nuclear extract containing increasing protein concentrations. Mutation analysis.
CUG-BP1CUGCUGCUGCUGCUGCUGCUGCUGCUGCUG-10Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
8912635Bhagavati S, Ghatpande A, Leung B. (1996)
Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy.
Biochem Biophys Res Commun. 228(1):55-62.
 Synthesized sequences EMSA, UV-crosslink, protein purification
CUG-BP1CUCUCUCUCUCUCUCUCUCUCUCUCUCUCU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
8912635Bhagavati S, Ghatpande A, Leung B. (1996)
Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy.
Biochem Biophys Res Commun. 228(1):55-62.
 Synthesized sequences EMSA, UV-crosslink, protein purification
CUG-BP1UUUUUUUGUUGUGUUUUUUCCUU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
8912635Bhagavati S, Ghatpande A, Leung B. (1996)
Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy.
Biochem Biophys Res Commun. 228(1):55-62.
 Synthesized sequences EMSA, UV-crosslink, protein purification
CUG-BP1UGUGUGUGUGUGUGUGUGUGUGUUUUU-4Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
CUG-BP1UGUGUGUGUGUGUGUGUGUGUGUGUUUUU-6Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
CUG-BP1UGUGUGUGUGUGUGUGUGUGUGUGUGUUUUU-8Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
CUG-BP1UGUGUGUGUGUGUGUGUGUGUGUGUGUGUG-10Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1UGUGUGUGUGUGUAUGUGUGUGUGUGUGUG-9Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1UAUGUAUGUAUGUAUGUAUGUAUGUAUGUA-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1GUUGGUUGGUUGGUUGGUUGGUUGGUUGGU-3Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1UGGUGGUGGUGGUGGUGGUGGUGGUGGUGG-3Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1UUGUUGUUGUUGUUGUUGUUGUUGUUGUUG-3Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1CUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUG-1Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18039683Mori D, Sasagawa N, Kino Y, Ishiura S. (2008)
Quantitative analysis of CUG-BP1 binding to RNA repeats.
J Biochem. 143(3):377-383.
 Synthesized sequences Surface plasmon resonance (SPR) with recombinant protein.
CUG-BP1UUUCUUGUUUGUUUGUUUGGGU-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18243120Vlasova IA, Tahoe NM, Fan D, Larsson O, Rattenbacher B, Sternjohn JR, Vasdewani J, Karypis G, Reilly CS, Bitterman PB, Bohjanen PR. (2008)
Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1.
Mol Cell. 29(2):263-270.
 Sequences deriving from wt and mutated JUN [3725], JUNB [3726], TNFRSF1B [7133]. Mutagenesis, EMSA with cytoplasmic extracts and supershift, UV cross-linking, siRNA-knockdown in primary human T cells and HeLa.
CUG-BP1GUGUUUGUGUUUGUGUGUGUUUGUU-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18243120Vlasova IA, Tahoe NM, Fan D, Larsson O, Rattenbacher B, Sternjohn JR, Vasdewani J, Karypis G, Reilly CS, Bitterman PB, Bohjanen PR. (2008)
Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1.
Mol Cell. 29(2):263-270.
 Sequences deriving from wt and mutated JUN [3725], JUNB [3726], TNFRSF1B [7133]. Mutagenesis, EMSA with cytoplasmic extracts and supershift, UV cross-linking, siRNA-knockdown in primary human T cells and HeLa.
CUG-BP1GUUUGUUUGUUUGUUUGUUUGUUU-5Gene Name and Synonymous: CUGBP1, CUG triplet repeat RNA binding protein 1, CUGBP, NAB50, CUG-BP, hNab50, BRUNOL2.
Note that CUGBP1 induces exon 5 inclusion in cTNT gene (PMID:9563950), induces exon 11 exclusion in IR gene (PMID:11528389), induces intron 2 retention in CIC-1 gene (PMID:12150906).
18243120Vlasova IA, Tahoe NM, Fan D, Larsson O, Rattenbacher B, Sternjohn JR, Vasdewani J, Karypis G, Reilly CS, Bitterman PB, Bohjanen PR. (2008)
Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1.
Mol Cell. 29(2):263-270.
 Sequences deriving from wt and mutated JUN [3725], JUNB [3726], TNFRSF1B [7133]. Mutagenesis, EMSA with cytoplasmic extracts and supershift, UV cross-linking, siRNA-knockdown in primary human T cells and HeLa.
DAZAP1UUUUUUU-7Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 10857750Tsui S, Dai T, Roettger S, Schempp W, Salido EC, Yen PH. (2000)
Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1.
Genomics. 65(3):266-273.
DAZAP1 has no affinity to poly (C). Homopolymers In vitro RNA-binding assay of homopolymers and SDS-PAGE with recombinant protein.
DAZAP1GGGGGGG-7Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 10857750Tsui S, Dai T, Roettger S, Schempp W, Salido EC, Yen PH. (2000)
Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1.
Genomics. 65(3):266-273.
DAZAP1 has no affinity to poly (C). Homopolymers In vitro RNA-binding assay of homopolymers and SDS-PAGE with recombinant protein.
DAZAP1AAAAAAA-3Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 10857750Tsui S, Dai T, Roettger S, Schempp W, Salido EC, Yen PH. (2000)
Identification of two novel proteins that interact with germ-cell-specific RNA-binding proteins DAZ and DAZL1.
Genomics. 65(3):266-273.
DAZAP1 has no affinity to poly (C). Homopolymers In vitro RNA-binding assay of homopolymers and SDS-PAGE with recombinant protein.
DAZAP1AGAAC-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 18503770Skoko N, Baralle M, Buratti E, Baralle FE. (2008)
The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors.
FEBS Lett. 582(15):2231-2236.
 Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37.In vivo splicing in HeLa, siRNA.UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts.
DAZAP1UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
DAZAP1UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
DAZAP1UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
DAZAP1UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
DAZAP1UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
DAZAP1UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA-5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
DAZAP1UGCAGAUGCUUAGUUUGUGU-8Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
DAZAP1UGCAGAUGGUUAGUUUGUGU-10Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
DAZAP1AGAUAU8Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
DAZAP1AAUUUA8Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
DAZAP1AGUAGG5Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
DAZAP1GUAACG8Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
DAZAP1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-6Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
DAZAP1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-4Gene Name and Synonymous: DAZAP1, DAZ associated protein 1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
ESRP1GUGUGGUGAUGGGCCUGCAGAGGUGAGCUGGCCGGUGUCUCUC5Gene Name and Synonymous: ESRP1, epithelial splicing regulatory protein 1, RBM35A, RMB35A, FLJ20171
It promotes exon inclusion or exclusion depending on epithelial or mesenchymal cells.
19285943Warzecha CC, Sato TK, Nabet B, Hogenesch JB, Carstens RP. (2009)
ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing.
Mol Cell. 33(5):591-601.
 Sequences deriving from wt and mutated rat Fgfr2 [25022].In vivo splicing in PNT2.Pull-down and immunoblotting with KATO III NE
ESRP1AGGGAU5Gene Name and Synonymous: ESRP1, epithelial splicing regulatory protein 1, RBM35A, RMB35A, FLJ20171
It promotes exon inclusion or exclusion depending on epithelial or mesenchymal cells.
20526337Dominguez C, Fisette JF, Chabot B, Allain FH. (2010)
Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs.
Nat Struct Mol Biol. 17(7):853-861.
 Synthesized sequences. Constructs of wt and mutant BCL2L1 [598] EX2 - INT2 - EX3.In vitro splicing assays in HeLa nuclear extracts.NMR spectroscopy. In vitro splicing assays in HeLa nuclear extracts with increasing amount of splicing factor.
ESRP2GUGUGGUGAUGGGCCUGCAGAGGUGAGCUGGCCGGUGUCUCUC5Gene Name and Synonymous: ESRP2, epithelial splicing regulatory protein 2, RBM35B, FLJ21918, FLJ22248
It promotes exon inclusion or exclusion depending on epithelial or mesenchymal cells.
19285943Warzecha CC, Sato TK, Nabet B, Hogenesch JB, Carstens RP. (2009)
ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing.
Mol Cell. 33(5):591-601.
 Sequences deriving from wt and mutated rat Fgfr2 [25022].In vivo splicing in PNT2.Pull-down and immunoblotting with KATO III NE
ETR-3AUGUG7Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3AUGUU8Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3CGUGU6Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3GUCUGU4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3GUGUG8Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3GUUGU8Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UAUGU9Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UGUGU7Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UGUUC6Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UGUUG7Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UGUUU6Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UUGUU6Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3CAUCG2Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3CUGCUGCUGCUGCUGCUGCUGCUG5Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
8948631Timchenko LT, Miller JW, Timchenko NA, DeVore DR, Datar KV, Lin L, Roberts R, Caskey CT, Swanson MS. (1996)
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
Nucleic Acids Res. 24(22): 4407-4414.
 Synthesized sequences. Purification of HeLa extract by DEAE chromatography and detection by bandshift/supershift analysis.
ETR-3GUGUGU7Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3AUGUGU7Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UGUGUG7Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UGUUGU6Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3GUAUGU4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3GUUGUU4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UAUGUG4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UAUGUU4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3UUGUGU4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
15657417Faustino NA, Cooper TA. (2005)
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Mol Cell Biol. 25 (3): 879-887.
 Sequences of 20nt random for SELEX. SELEX of 20nt random with recombinant protein, some winner sequences are verified by EMSA.
ETR-3CUGCUGCUGCUGCUGCUGCUGCUGCUGCUG-10Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
8912635Bhagavati S, Ghatpande A, Leung B. (1996)
Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy.
Biochem Biophys Res Commun. 228(1):55-62.
 Synthesized sequences EMSA, UV-crosslink, protein purification
ETR-3CUCUCUCUCUCUCUCUCUCUCUCUCUCUCU-8Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
8912635Bhagavati S, Ghatpande A, Leung B. (1996)
Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy.
Biochem Biophys Res Commun. 228(1):55-62.
 Synthesized sequences EMSA, UV-crosslink, protein purification
ETR-3UUUUUUUGUUGUGUUUUUUCCUU-8Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
8912635Bhagavati S, Ghatpande A, Leung B. (1996)
Identification of two nuclear proteins which bind to RNA CUG repeats: significance for myotonic dystrophy.
Biochem Biophys Res Commun. 228(1):55-62.
 Synthesized sequences EMSA, UV-crosslink, protein purification
ETR-3UGUGUGUGUGUGUGUGUGUGUGUUUUU-4Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
ETR-3UGUGUGUGUGUGUGUGUGUGUGUGUUUUU-6Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
ETR-3UGUGUGUGUGUGUGUGUGUGUGUGUGUUUUU-8Gene Name and Synonymous: CUGBP2, CUG triplet repeat RNA binding protein 2, NAPOR, BRUNOL3.
Note that ETR-3 induces exon 5 inclusion in cTNT gene (PMID:11931771), induces exon 9 inclusion in CFTR gene (PMID:15657417), promotes selectively the exclusion of Tau exon 2 (PMID:16862542).
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
FMRPGAAGAGGACAAGGAGGAAGAGGACGUGGAGGAGGC5Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11532944Schaeffer C, Bardoni B, Mandel JL, Ehresmann B, Ehresmann C, Moine H. (2001)
The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif.
EMBO J. 20(17): 4803-4813.
This long motif form a correct G-quartet structure. Synthesized sequences. EMSA, competition assay, ladder selection with recombinant protein.
FMRPAAGAGAGGGAGAGCUUCCUGCGCAGAGGAGACGGACGGCGGCGUGGAGGGGGAGGAAGAGGACAAGGAGGAAGAGGACGUGGAGGAGGCUUCAAAGGAAAC10Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11532944Schaeffer C, Bardoni B, Mandel JL, Ehresmann B, Ehresmann C, Moine H. (2001)
The fragile X mental retardation protein binds specifically to its mRNA via a purine quartet motif.
EMBO J. 20(17): 4803-4813.
This long motif form a correct G-quartet structure. Synthesized sequences. EMSA, competition assay, ladder selection with recombinant protein.
FMRPGGAGGAAGAGGACAAGGAGGAAGAGGACGUGGAGGAGGCUUCAAA5Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
18653529Didiot MC, Tian Z, Schaeffer C, Subramanian M, Mandel JL, Moine H. (2008)
The G-quartet containing FMRP binding site in FMR1 mRNA is a potent exonic splicing enhancer.
Nucleic Acids Res. 36(15): 4902-4912.
This short motif is still able to form a G-quartet structure. Construct of beta-globin [3043] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4, with putative and mutant ESEs inserted in EX2. In vivo splicing in HeLa cells.Site directed mutagenesis, EMSA, competition assay.
FMRPGCUGCGGUGUGGAAGGAGUGGCUGGGUUGCGCAGC10Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11719189Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001)
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
Cell. 107(4): 489-499.
Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. Synthesized sequences. Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein.
FMRPAAGGGUAGGAUGGGAUGGCUGGCGAGG2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11719189Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001)
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
Cell. 107(4): 489-499.
Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. Synthesized sequences. Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein.
FMRPAAGGUAGGGUGGUUGGG2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11719189Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001)
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
Cell. 107(4): 489-499.
Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. Synthesized sequences. Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein.
FMRPGUGGGUGGUUGGGUGG2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11719189Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001)
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
Cell. 107(4): 489-499.
Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. Synthesized sequences. Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein.
FMRPGAGGAGUUGGAAGGAUGGG2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
11719189Darnell JC, Jensen KB, Jin P, Brown V, Warren ST, Darnell RB. (2001)
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
Cell. 107(4): 489-499.
Binding site with score 2 are not confermed by RNase T1 protection assay. Binding site may be longer, so able to form G-quartet structure. Synthesized sequences. Column RNA affinity, mutational analysis, RNase T1 protection assay, nitrocellulose filter binding assays, EMSA supershift with recombinant protein.
FMRPAAGCGGCUGG10Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGAGCGAAGGGAG7Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGAGCGACUGG6Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGAGCGACUGGUG2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGAGCGGCUGG7Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGACUAAGGAGU8Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCAUAGGCAC8Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCGAAGGAAG10Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCGAAGGAGU9Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCGAAGGGAG10Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUAAGGAAU6Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUAAGGAGU2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUAAGGAUU7Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUAUGGAGU6Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUGAAGGAAC8Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUGAAGGAAG4Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGCUGAGGAUG6Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGUGCGACUGGGC4Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGUGCGGCUGGGC8Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPUAGCAGCUGG7Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPUAGCGACUGG10Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPUAGCGGCUGG7Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPUAGUGGCUGG8Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
15805463Darnell JC, Fraser CE, Mostovetsky O, Stefani G, Jones TA, Eddy SR, Darnell RB. (2005)
Kissing complex RNAs mediate interaction between the Fragile-X mental retardation protein KH2 domain and brain polyribosomes.
Genes Dev. 19(8): 903-918.
 Sequences of 52nt random for SELEX. SELEX of 52nt random with recombinant protein. Filter binding assays and EMSA supershift with recombinant protein.
FMRPGGGGUUGGGGAUUUAGCUCAGUGGUAGAGCGCUUGCCUAGCAAGCGCAAGGCCCUGGGUUCGGUCCUCAGCUCUGG5Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
16006558Zalfa F, Adinolfi S, Napoli I, Kuhn-Holsken E, Urlaub H, Achsel T, Pastore A, Bagni C. (2005)
Fragile X mental retardation protein (FMRP) binds specifically to the brain cytoplasmic RNAs BC1/BC200 via a novel RNA-binding motif.
J Biol Chem. 280(39): 33403-33410.
 Synthesized sequences, Rodent brain-specific BC1 RNA. EMSA, competition assay with recombinant protein.
FMRPGGGGGGG7Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
7688265Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. (1993)
The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein.
Cell. 74(2):291-298.
 Homopolymers Homopolymer binding assay with recombinant protein
FMRPUUUUUUU4Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
7688265Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. (1993)
The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein.
Cell. 74(2):291-298.
 Homopolymers Homopolymer binding assay with recombinant protein
FMRPGGCUGCGGUGUGGAAGGUGAGGCUGGGUUGCGCAGCU5Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
16819831Zanotti KJ, Lackey PE, Evans GL, Mihailescu MR. (2006)
Thermodynamics of the fragile X mental retardation protein RGG box interactions with G quartet forming RNA.
Biochemistry. 45(27):8319-8330.
 Synthesized sequences EMSA with recombinant protein. Fluorescence and Circular Dichroism Spectroscopy.
FMRPAAAAAAA-2Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
8917439Dejgaard K, Leffers H. (1996)
Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains.
Eur J Biochem. 241(2):425-431.
 Synthesized oligos Homopolymer binding assay with recombinant protein.
FMRPGGCUGGUGAUUGGAAGGGAGGGAGGUGGCCAGCC5Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
17693432Menon L, Mihailescu MR. (2007)
Interactions of the G quartet forming semaphorin 3F RNA with the RGG box domain of the fragile X protein family.
Nucleic Acids Res. 35(16):5379-5392.
 Synthesized sequences EMSA supershift with recombinant protein. Fluorescence, UV and CD spectroscopy.
FMRPGGAUUGGAAGGGAGGGAGGUG5Gene Name and Synonymous: FMR1, fragile X mental retardation 1, POF, POF1, FRAXA, MGC87458, FMR1.
FMRP binds to its own mRNA in vitro, particularly a G-quartet motif (11532944), acting as a potent ESE (PMID: 18653529). It can also bind RNA using its kissing-loop motif (PMID: 15805463).
19396385Bole M, Menon L, Mihailescu MR. (2008)
Fragile X mental retardation protein recognition of G quadruplex structure per se is sufficient for high affinity binding to RNA.
Mol Biosyst. 4(12):1212-1219.
 Synthesized sequences Fluorescence, UV and CD spectroscopy with recombinant protein.
Fox-1AGCAUG1Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1.
Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
16537540Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006)
Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16.
J Biol Chem. 281(18): 12468-12474.
 Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay.
Fox-1UGCAUG10Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1.
Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
16537540Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006)
Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16.
J Biol Chem. 281(18): 12468-12474.
 Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay.
Fox-1UGCAUG-5Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1.
Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
17101796Zhou HL, Baraniak AP, Lou H. (2007)
Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.
Mol Cell Biol. 27(3): 830-841.
 Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4.In vivo splicing in HeLa cell.siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation.
Fox-1UGACUG-5Gene Name and Synonymous: A2BP1, ataxin 2-binding protein 1, HRNBP1.
Fox-1 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
17101796Zhou HL, Baraniak AP, Lou H. (2007)
Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.
Mol Cell Biol. 27(3): 830-841.
 Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4.In vivo splicing in HeLa cell.siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation.
Fox-2UGCAUG10Gene Name and Synonymous: RBFOX2, RNA binding protein, fox-1 homolog (C. elegans) 2, RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3.
Fox-2 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
16537540Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006)
Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16.
J Biol Chem. 281(18): 12468-12474.
 Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay.
Fox-2UGCAUG-5Gene Name and Synonymous: RBFOX2, RNA binding protein, fox-1 homolog (C. elegans) 2, RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3.
Fox-2 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
17101796Zhou HL, Baraniak AP, Lou H. (2007)
Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.
Mol Cell Biol. 27(3): 830-841.
 Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4.In vivo splicing in HeLa cell.siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation.
Fox-2UGACUG-5Gene Name and Synonymous: RBFOX2, RNA binding protein, fox-1 homolog (C. elegans) 2, RTA, fxh, FOX2, RBM9, Fox-2, HNRBP2, HRNBP2, dJ106I20.3.
Fox-2 can act both as splicing enhancer (PMID: 16537540) and as silencer (PMID: 17101796).
17101796Zhou HL, Baraniak AP, Lou H. (2007)
Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.
Mol Cell Biol. 27(3): 830-841.
 Construct of wt and mutant calcitonin/CGRP CALCA [796] Ad_EX - INT3 - EX4.In vivo splicing in HeLa cell.siRNA-mediated knockdown, UV cross-linking with HeLa nuclear extract and immunoprecipitation.
hnRNP A0AUUUA-3Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. 7585247Myer VE, Steitz JA. (1995)
Isolation and characterization of a novel, low abundance hnRNP protein: A0.
RNA. 1(2): 171-182.
 Sequence of 30nt. RNA affinity chromatography with HeLa nuclear extract confirmed by UV crosslink
hnRNP A0AGAUAU-8Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A0AAUUUA-10Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A0AGUAGG-9Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A0GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-6Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP A0AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-4Gene Name and Synonymous: HNRNPA0, heterogeneous nuclear ribonucleoprotein A0, HNRPA0. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP A1UAGGAA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1UAGAGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1UAGGAU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1UAGGUA-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1UAGGCU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1CAGGGA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1UAGGGA-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1UAGGGU-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
7510636Burd CG, Dreyfuss G. (1994)
RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing.
EMBO J. 13(5): 1197-1204.
 Construct of major late transcription unit of adenovirus 2 EX1 - INT1 - EX2 for in vitro splicing assay. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear extracts.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by filter binding assays with purified protein. Confirmed by UV-crosslink and immunoblot with HeLa nuclear extract
hnRNP A1AUUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
8473331Hamilton BJ, Nagy E, Malter JS, Arrick BA, Rigby WF. (1993)
Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences.
J Biol Chem. 268(12): 8881-7.
 Partial sequence of colony stimulating factor 2 CSF2 [1437] 3'UTR. UV crosslink and immunoprecipitation with cytoplasmic lysates of lymphocytes.
hnRNP A1UAGACA-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12833158Kashima T, Manley JL. (2003)
A negative element in SMN2 exon 7 inhibits splicing in spinal muscular atrophy.
Nat Genet. 34(4): 460-463.
 Constructs and mutants of SMN1 [6606] and SMN2 [6607] EX7.In vivo splicing in HeLa.UV crosslink, SDS-PAGE, immunoprecipitation, siRNA and Western blot.
hnRNP A1AUAGAA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12419255Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, Branlant C. (2002)
A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding.
J Mol Biol. 323(4): 629-652.
 Constructs of HIV-1 A7 3' splice siteIn vitro splicing with HeLa cell nuclear or cytoplasmic S100 extracts.Competition assay, UV crosslink, immunoprecipitation with HeLa nuclear extracts, EMSA and supershift assays.
hnRNP A1CUAGACUAGA-6Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
10406810Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999)
hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing.
EMBO J. 18(14):4060-4067.
 Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay.RNA affinity chromatography, immunoblotting.
hnRNP A1AGAAC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18503770Skoko N, Baralle M, Buratti E, Baralle FE. (2008)
The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors.
FEBS Lett. 582(15):2231-2236.
 Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37.In vivo splicing in HeLa, siRNA.UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts.
hnRNP A1GGAGGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP A1UAGGGCAGGC-6Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9858549Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999)
hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer.
Mol Cell Biol. 19(1):251-260.
 Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing.In vivo splicing in HeLa, SVK14 and 293 cells.UV crosslink, immunoprecipitation in HeLa cell nuclear extracts.
hnRNP A1UAGGGC-6Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9858549Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999)
hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer.
Mol Cell Biol. 19(1):251-260.
 Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing.In vivo splicing in HeLa, SVK14 and 293 cells.UV crosslink, immunoprecipitation in HeLa cell nuclear extracts.
hnRNP A1UAGAGU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9121425Chabot B, Blanchette M, Lapierre I, La Branche H. (1997)
An intron element modulating 5' splice site selection in the hnRNP A1 pre-mRNA interacts with hnRNP A1.
Mol Cell Biol. 17(4):1776-1786.
 Construct of murine hnRNP A1 [15382] EX7 - 7b - partial_EX8.In vitro splicing assay in HeLa nuclear extracts. In vivo splicing assay using tranfected HeLa cells.RNase protection, immunoprecipitation in HeLa nuclear extract.
hnRNP A1UAGGCA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11598017Tange TO, Damgaard CK, Guth S, Valcarcel J, Kjems J. (2001)
The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element.
EMBO J. 20(20):5748-5758.
 Construct of HIV-1 Tat [155871] INT2.In vitro splicing with HeLa nuclear extracts, immunodepletion.UV crosslink, immunoblot, EMSA.
hnRNP A1UAGUGA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11598017Tange TO, Damgaard CK, Guth S, Valcarcel J, Kjems J. (2001)
The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element.
EMBO J. 20(20):5748-5758.
 Construct of HIV-1 Tat [155871] INT2.In vitro splicing with HeLa nuclear extracts, immunodepletion.UV crosslink, immunoblot, EMSA.
hnRNP A1UUUUUUU-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
8449401Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B. (1993)
Cloning and characterization of PSF, a novel pre-mRNA splicing factor.
Genes Dev. 7(3):393-406.
PSF has no affinity to poly(rA), poly(rC) and poly(rG). Construct of tropomyosin 1 alpha TPM1 [7168] EX2 - INT2 - EX3 for splicing. Construct of branchpoint and polypyrimidine tract element upstream of TPM1 EX3 for UV-crosslink.In vitro splicing in HeLa nuclear extracts.RNA affinity chromatography confirmed by UV crosslink and Western blot using HeLa nuclear extracts.
hnRNP A1UUAGAUUAGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9275159Li HP, Zhang X, Duncan R, Comai L, Lai MM. (1997)
Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA.
Proc Natl Acad Sci U S A. 94(18): 9544-9549.
 Sequence of (-)-strand leader RNA of mouse hepatitis virus (MHV). Intergenic sequences of mouse hepatitis virus (MHV). UV crosslink with cytoplasmic extracts from HeLa cells. Protein identified by peptide sequencing and immunoprecipitation.
hnRNP A1GUUUAGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9275159Li HP, Zhang X, Duncan R, Comai L, Lai MM. (1997)
Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA.
Proc Natl Acad Sci U S A. 94(18): 9544-9549.
 Sequence of (-)-strand leader RNA of mouse hepatitis virus (MHV). Intergenic sequences of mouse hepatitis virus (MHV). UV crosslink with cytoplasmic extracts from HeLa cells. Protein identified by peptide sequencing and immunoprecipitation.
hnRNP A1CAAGCACCGAACCCGCAACUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A1GCCAAGGAGCCAGAGAGCAUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A1GCCCUUGGGUUUGCAUGCCACUGCAUGAGAGACGUUUAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16537540Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006)
Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16.
J Biol Chem. 281(18): 12468-12474.
 Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay.
hnRNP A1GCCCUUGGGUUUGACUGCCACUGACUGAGAGACGUUUAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16537540Ponthier JL, Schluepen C, Chen W, Lersch RA, Gee SL, Hou VC, Lo AJ, Short SA, Chasis JA, Winkelmann JC, Conboy JG. (2006)
Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16.
J Biol Chem. 281(18): 12468-12474.
 Construct of EPB41 [2035] from EX13 to EX17 for splicing assay. Sequences of 40nt random for SELEX.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.SELEX of 40nt random with recombinant protein. Immunoblot, siRNA, pulldown assay.
hnRNP A1CGUAGGUC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP A1UGAAGUAGAUUAGCAUCUACUGCCCUAAGUGCUCCUUCUGGCA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17558416Guil S, Caceres JF. (2007)
The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a.
Nat Struct Mol Biol. 14(7): 591-596.
hnRNP A1 facilitates processing of miR-18a. HeLa endogenous RNAs. In vivo UV cross-linking and immunoprecipitation (CLIP) in HeLa cells.
hnRNP A1UAGGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP A1UAGUUAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP A1CUUAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP A1GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
hnRNP A1UCGGGC-3Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9858549Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999)
hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer.
Mol Cell Biol. 19(1):251-260.
 Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing.In vivo splicing in HeLa, SVK14 and 293 cells.UV crosslink, immunoprecipitation in HeLa cell nuclear extracts.
hnRNP A1UACGGC-3Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9858549Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999)
hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer.
Mol Cell Biol. 19(1):251-260.
 Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing.In vivo splicing in HeLa, SVK14 and 293 cells.UV crosslink, immunoprecipitation in HeLa cell nuclear extracts.
hnRNP A1UAGAUCCUAGACUAGAGCCCUG-6Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9858549Del Gatto-Konczak F, Olive M, Gesnel MC, Breathnach R. (1999)
hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer.
Mol Cell Biol. 19(1):251-260.
 Sequence and mutants of FGFR2 [2263] EX_KSAM, sequence and mutants of HIV1 tat [155871] EX2 for UV crosslink and immunoprecipitation. Constructs of FGFR2 EX_C1 - INT - EX_KSAM - INT - EX_BEK - INT - EX_C2 for in vivo splicing.In vivo splicing in HeLa, SVK14 and 293 cells.UV crosslink, immunoprecipitation in HeLa cell nuclear extracts.
hnRNP A1UAGGGACUUA-6Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
10406810Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999)
hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing.
EMBO J. 18(14):4060-4067.
 Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay.RNA affinity chromatography, immunoblotting.
hnRNP A1GAGUGGUGCG-6Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
10406810Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999)
hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing.
EMBO J. 18(14):4060-4067.
 Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay.RNA affinity chromatography, immunoblotting.
hnRNP A1GUAAGUACGC-3Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
10406810Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999)
hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing.
EMBO J. 18(14):4060-4067.
 Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay.RNA affinity chromatography, immunoblotting.
hnRNP A1UAGUGAA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11779509Zhu J, Mayeda A, Krainer AR. (2001)
Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins.
Mol Cell. 8(6): 1351-1361.
 Sequence of HIV-1 Tat [155871] EX3.In Vitro Splicing with HeLa S100 and HeLa nuclear extracts.UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot.
hnRNP A1AAGGUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP A1CUGAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP A1AUAGCA-9Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12426391Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002)
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
EMBO J. 21(22):6195-6204.
 Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells.RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting.
hnRNP A1UUUAUA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12426391Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002)
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
EMBO J. 21(22):6195-6204.
 Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells.RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting.
hnRNP A1AUUUAA-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12426391Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002)
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
EMBO J. 21(22):6195-6204.
 Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells.RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting.
hnRNP A1UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1CUGAUUUGUAUUUAUUAGACUC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1AACAGAAAAAGAAAUAUUU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1CUGAUUUGUACCUAUUAGAUUC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1UACUGAAGAACAAGUAUUU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A1CCAUGGUUUGGGGGCAGUAGUUGG-1Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP A1AAGAAUGGGAAUAUUUUAUACUGACAGAAAUCAGUAAUAUUUAUAUAUUUAUAUUUUUAAAAUAUUUAUUUAUUUAUUUAUUUAAGUUCAUAUUCCAUAUUUAUUCAAGAUGUUUUACCGUAAUAAUUAUUAUUAAAAAUAUGCUUCUAAA-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9353343Hamilton BJ, Burns CM, Nichols RC, Rigby WF. (1997)
Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation.
J Biol Chem. 272(45):28732-28741.
 Synthesized sequences and sequence deriving from CSF2 [1437] and HBB [3043] EMSA, UV cross-linking, immunoblotting, competition assay with human T lymphocytes extracts and recombinant protein
hnRNP A1GGAUCCAUUUAUUUAUUUAUUUAAGCUUGG-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9353343Hamilton BJ, Burns CM, Nichols RC, Rigby WF. (1997)
Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation.
J Biol Chem. 272(45):28732-28741.
 Synthesized sequences and sequence deriving from CSF2 [1437] and HBB [3043] EMSA, UV cross-linking, immunoblotting, competition assay with human T lymphocytes extracts and recombinant protein
hnRNP A1UUGGUAUCAAGGUUACAAGACAGGUUUAAGGAGACCAAUAGAAACUGGGCAUGUGGAGACAGAGAAGACUCUUGGGUUUCUGAUAGGCACUGACUCUCUCUGCCUAUUGGUCUAUUUUCCCACCCUUAG-3Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9353343Hamilton BJ, Burns CM, Nichols RC, Rigby WF. (1997)
Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation.
J Biol Chem. 272(45):28732-28741.
 Synthesized sequences and sequence deriving from CSF2 [1437] and HBB [3043] EMSA, UV cross-linking, immunoblotting, competition assay with human T lymphocytes extracts and recombinant protein
hnRNP A1UAGGUUAGGAAUAGGGAAUUAAGG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9740129Mayeda A, Munroe SH, Xu RM, Krainer AR. (1998)
Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1.
RNA. 4(9):1111-1123.
 Synthesized sequences Filter binding assay with recombinant protein
hnRNP A1UUAGAUCGAUGGGAAAAAAUUCGGUUAAGG-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9925777Najera I, Krieg M, Karn J. (1999)
Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1.
J Mol Biol. 285(5):1951-1964.
 p17gag [155030] and synthesized sequences. Mutation analysis, EMSA supershift and UV cross-linking in HeLa NE.
hnRNP A1UAUGAUAGGGACUUAGGGUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
9925777Najera I, Krieg M, Karn J. (1999)
Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1.
J Mol Biol. 285(5):1951-1964.
 p17gag [155030] and synthesized sequences. Mutation analysis, EMSA supershift and UV cross-linking in HeLa NE.
hnRNP A1CUAGUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1GAAGUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1CUUCUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1CUAGUACUAACUAAACUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1CUAGUAGAUUCUAAACUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1CUAGUAGAAACUAUUCUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1GAUCUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1AGCUAGAUUAGACUUC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A1AGCUAAGACUGGGGGCAGGAUGGCGGAAAGGAAGGGGCGUGGUGGCUAGAGGGAAGAGAAGAAACAGAAGCGGCUCAGUUCACCUUGAUAAGGUGCUUCCGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
15155834Christian K, Lang M, Maurel P, Raffalli-Mathieu F. (2004)
Interaction of heterogeneous nuclear ribonucleoprotein A1 with cytochrome P450 2A6 mRNA: implications for post-transcriptional regulation of the CYP2A6 gene.
Mol Pharmacol. 65(6):1405-1414.
 Sequences deriving from CYP2A6 [1548] UV cross-linking in primary hepatocyte NE and HepG2 NE
hnRNP A1AGGGUUGAGGGGAGCAGGGU-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP A1AUUUAUUU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
15514164Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004)
RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis.
Circ Res. 95(11):1058-1066.
 Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF.
hnRNP A1GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP A1UAGGGAUAGGGUUAGGGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16157593Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005)
TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing.
J Biol Chem. 280(45):37572-37584.
 Synthesized sequences EMSA with recombinant protein
hnRNP A1GGUUUCAGACAAAAUCA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16385450Cartegni L, Hastings ML, Calarco JA, de Stanchina E, Krainer AR. (2006)
Determinants of exon 7 splicing in the spinal muscular atrophy genes, SMN1 and SMN2.
Am J Hum Genet. 78(1):63-77.
 Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 In vitro splicing in HeLa RNA affinity chromatography with HeLa NE, western blot.
hnRNP A1GGUUUUAGACAAAAUCA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16385450Cartegni L, Hastings ML, Calarco JA, de Stanchina E, Krainer AR. (2006)
Determinants of exon 7 splicing in the spinal muscular atrophy genes, SMN1 and SMN2.
Am J Hum Genet. 78(1):63-77.
 Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 In vitro splicing in HeLa RNA affinity chromatography with HeLa NE, western blot.
hnRNP A1GAGGAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16990281Hallay H, Locker N, Ayadi L, Ropers D, Guittet E, Branlant C. (2006)
Biochemical and NMR study on the competition between proteins SC35, SRp40, and heterogeneous nuclear ribonucleoprotein A1 at the HIV-1 Tat exon 2 splicing site.
J Biol Chem. 281(48):37159-37174.
 Sequences deriving from HIV-1 Tat [155871] Competition assays with recombinant protein
hnRNP A1UAUAAUGUUCUCUUUUUAGAAAAGGU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17287399Lewis SM, Veyrier A, Hosszu Ungureanu N, Bonnal S, Vagner S, Holcik M. (2007)
Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translation.
Mol Biol Cell. 18(4):1302-1311.
 Sequences deriving from XIAP [331] UV cross-linking, competition assay with recombinant protein
hnRNP A1GGACAAGUCCUAUUUUCAAGAGAAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17287399Lewis SM, Veyrier A, Hosszu Ungureanu N, Bonnal S, Vagner S, Holcik M. (2007)
Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translation.
Mol Biol Cell. 18(4):1302-1311.
 Sequences deriving from XIAP [331] UV cross-linking, competition assay with recombinant protein
hnRNP A1CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP A1CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP A1GAGAAUUCGCUGGUCUCGAACUCCUGACCUCAAGUGAUCCCACCGAAUUCGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17353911Donev R, Newall A, Thome J, Sheer D. (2007)
A role for SC35 and hnRNPA1 in the determination of amyloid precursor protein isoforms.
Mol Psychiatry. 12(7):681-690.
 Synthesized sequences EMSA with recombinant protein
hnRNP A1GAGAAUUCGCUCCUGACCUCAAGUGAUCCCACCGAAUUCGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17353911Donev R, Newall A, Thome J, Sheer D. (2007)
A role for SC35 and hnRNPA1 in the determination of amyloid precursor protein isoforms.
Mol Psychiatry. 12(7):681-690.
 Synthesized sequences EMSA with recombinant protein
hnRNP A1CAGAAGGGGAGGGGUUCCA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP A1AACAAGGGGUGGGGGAAAA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP A1AUGUCUCUUUGGCUGCCUAGUGAGGCCACUGUCUACUUGCCUCCUGUCCCAGUAUCUAAGGUUGUAAGCACGGAUGAAUAUGUUGCACGCACAAACAUAUAUUAUCAUGCAGGAACAUCCAGACUACUU-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17869320Zhao X, Fay J, Lambkin H, Schwartz S. (2007)
Identification of a 17-nucleotide splicing enhancer in HPV-16 L1 that counteracts the effect of multiple hnRNP A1-binding splicing silencers.
Virology. 369(2):351-363.
 Sequences deriving from HPV-16 L1 mRNA and synthesized sequences. UV cross-linking with HeLa NE and recombinant protein
hnRNP A1UAGUGUAGUGUAGUGUAGUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17869320Zhao X, Fay J, Lambkin H, Schwartz S. (2007)
Identification of a 17-nucleotide splicing enhancer in HPV-16 L1 that counteracts the effect of multiple hnRNP A1-binding splicing silencers.
Virology. 369(2):351-363.
 Sequences deriving from HPV-16 L1 mRNA and synthesized sequences. UV cross-linking with HeLa NE and recombinant protein
hnRNP A1AGACCUUUAGGGUUAGGGACAUCC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17925491Eiring AM, Neviani P, Santhanam R, Oaks JJ, Chang JS, Notari M, Willis W, Gambacorti-Passerini C, Volinia S, Marcucci G, Caligiuri MA, Leone GW, Perrotti D. (2008)
Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis.
Blood. 111(2):816-828.
 Sequences deriving from E2F3 [1871] and synthesized sequences. EMSA, UV cross-linking, competition assays, western blot with K562 cytoplasmic extracts.
hnRNP A1AGACCUCUAGGGAGAAAGACAUCC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17925491Eiring AM, Neviani P, Santhanam R, Oaks JJ, Chang JS, Notari M, Willis W, Gambacorti-Passerini C, Volinia S, Marcucci G, Caligiuri MA, Leone GW, Perrotti D. (2008)
Identification of novel posttranscriptional targets of the BCR/ABL oncoprotein by ribonomics: requirement of E2F3 for BCR/ABL leukemogenesis.
Blood. 111(2):816-828.
 Sequences deriving from E2F3 [1871] and synthesized sequences. EMSA, UV cross-linking, competition assays, western blot with K562 cytoplasmic extracts.
hnRNP A1GCUAAACGCAAAAAACGUAAGCUGUAAGUAUUGUAUGUAUGUUGAAUUAGUGUUGUUUGUUGUGUAUAUGUUUGUAUGU-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
17950949Cheunim T, Zhang J, Milligan SG, McPhillips MG, Graham SV. (2008)
The alternative splicing factor hnRNP A1 is up-regulated during virus-infected epithelial cell differentiation and binds the human papillomavirus type 16 late regulatory element.
Virus Res. 131(2):189-198.
 Sequences deriving from HPV-16 LRE. EMSA supershift, UV cross-linking with HeLa NE and W12E NE. EMSA with recombinant protein.
hnRNP A1CAGCAUUAUGAAAG-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A1CCGCAUUAUGAAAG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A1CAGCAUUAUGAACG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A1CCGCAUUAUGAACG-1Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A1UGCAGAUGCUUAGUUUGUGU-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
hnRNP A1UGCAGAUGGUUAGUUUGUGU-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
hnRNP A1CCCCCCC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
3733753Kumar A, Williams KR, Szer W. (1986)
Purification and domain structure of core hnRNP proteins A1 and A2 and their relationship to single-stranded DNA-binding proteins.
J Biol Chem. 261(24):11266-11273.
 Synthesized oligos Filter binding assay with purified protein
hnRNP A1AAAAAAA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
3733753Kumar A, Williams KR, Szer W. (1986)
Purification and domain structure of core hnRNP proteins A1 and A2 and their relationship to single-stranded DNA-binding proteins.
J Biol Chem. 261(24):11266-11273.
 Synthesized oligos Filter binding assay with purified protein
hnRNP A1GUAUCCUUCCCUGGCCGUGAUAGUAGAGCAGCAGGGCCAGGGGGGUGACACACC-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP A1AGGUAGGGCCCUAAGGGCA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
20010808David CJ, Chen M, Assanah M, Canoll P, Manley JL. (2010)
HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer.
Nature. 463(7279):364-368.
 Sequences deriving from PKM2 [5315]. Construct of PKM2 [5315] EX9 - INT9 - AdML. Construct of PKM2 [5315] EX8 - INT8 - EX9 - INT9 - EX10 - INT10 - EX11.In vitro splicing with HeLa NE. In vivo splicing in HeLa.Protein affinity purification, mass spectrometry, UV cross-linking and immunoblotting with HeLa NE. siRNA.
hnRNP A1AGGUAGGGCCCUAAGGGCA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
20010808David CJ, Chen M, Assanah M, Canoll P, Manley JL. (2010)
HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer.
Nature. 463(7279):364-368.
 Sequences deriving from PKM2 [5315]. Construct of PKM2 [5315] EX9 - INT9 - AdML. Construct of PKM2 [5315] EX8 - INT8 - EX9 - INT9 - EX10 - INT10 - EX11.In vitro splicing with HeLa NE. In vivo splicing in HeLa.Protein affinity purification, mass spectrometry, UV cross-linking and immunoblotting with HeLa NE. siRNA.
hnRNP A1AUAUAUUUAAUCUUAAUCUGUUUAUUUACAAGGGAAGAUUUAUGUUUGGUGAACUAUAUUA-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18846111Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. (2009)
hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA.
Cell Death Differ. 16(2):244-252.
 Sequences deriving fromwt and mutated BIRC2 [329] RNA-af?nity chromatography, western blot with HEK293 protein extracts. UV cross-linking with recombinant protein.
hnRNP A1AUAUAGCAAUCUUAAUCUGUUUAUUUACAAGGGAAGAUUUAUGUUUGGUGAACUAUAUUA-3Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18846111Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. (2009)
hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA.
Cell Death Differ. 16(2):244-252.
 Sequences deriving fromwt and mutated BIRC2 [329] RNA-af?nity chromatography, western blot with HEK293 protein extracts. UV cross-linking with recombinant protein.
hnRNP A1AUAUAUUUAAUCUUAAUCUGUUUAGCACAAGGGAAGAUUUAUGUUUGGUGAACUAUAUUA-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18846111Zhao TT, Graber TE, Jordan LE, Cloutier M, Lewis SM, Goulet I, Cote J, Holcik M. (2009)
hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA.
Cell Death Differ. 16(2):244-252.
 Sequences deriving fromwt and mutated BIRC2 [329] RNA-af?nity chromatography, western blot with HEK293 protein extracts. UV cross-linking with recombinant protein.
hnRNP A1CAAAAAGAAGGAAGGUGCUCACAU-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19953646Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010)
A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy.
Hum Mutat. 31(1):E1110-1125.
 Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein
hnRNP A1CAAAAAGAACGAAGGUGCUCACAU-4Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
19953646Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010)
A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy.
Hum Mutat. 31(1):E1110-1125.
 Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein
hnRNP A1UAUGAUAGGCUCAUAGGGUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UAUGAUAGGCACUUAGGCUG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UUAGAUCGAUGGGAAAAAAUUCGUUAAGG-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UUAGAUCGAUCCCAAAAAAUUCGUUAAGG-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UAAAUGUGGGGACCUAGAGGAGGAGCUGAAAAUUGUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UAAACUACGCGACCUAGAGGAGGAGCUGAAAAUUGUUA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UAUGACCCGGACUCCCGGUG-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1UGCAAUGUACUUGCAAACAAUGGCCUGAGUGUGCAAAGAAAAUGUCUGCUAACUGC-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP A1AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP A1AUUUUCCUUACAGGGUUUCAGACAAAAUCAAAAAG-4Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP A1GAAUGGCCGGAGGAGAUUCAGCCUGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
20120036Homolova K, Zavadakova P, Doktor TK, Schroeder LD, Kozich V, Andresen BS. (2010)
The deep intronic c.903+469T>C mutation in the MTRR gene creates an SF2/ASF binding exonic splicing enhancer, which leads to pseudoexon activation and causes the cblE type of homocystinuria.
Hum Mutat. 31(4):437-444.
 Sequences deriving from wt or mutated MTRR [4552]. Constructs of wt or mutated HBB [3043]_EX1 - MTRR [4552]_INT6 - HBB [3043]_EX2 and Tat [155871]_EX1 - MTRR [4552]_INT6 - Tat [155871]_EX2.In vivo splicing in HEK293, protein overexpression and siRNA knockdownPulldown and Western blotting with HeLa NE
hnRNP A1GAAUGGCUGGAGGAGAUUCAGCCUGA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
20120036Homolova K, Zavadakova P, Doktor TK, Schroeder LD, Kozich V, Andresen BS. (2010)
The deep intronic c.903+469T>C mutation in the MTRR gene creates an SF2/ASF binding exonic splicing enhancer, which leads to pseudoexon activation and causes the cblE type of homocystinuria.
Hum Mutat. 31(4):437-444.
 Sequences deriving from wt or mutated MTRR [4552]. Constructs of wt or mutated HBB [3043]_EX1 - MTRR [4552]_INT6 - HBB [3043]_EX2 and Tat [155871]_EX1 - MTRR [4552]_INT6 - Tat [155871]_EX2.In vivo splicing in HEK293, protein overexpression and siRNA knockdownPulldown and Western blotting with HeLa NE
hnRNP A1UUUAGUCAGCCUUAUAGCUAA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
hnRNP A1GAGGAAG5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
23430061Oh HK, Lee E, Jang HN, Lee J, Moon H, Sheng Z, Jun Y, Loh TJ, Cho S, Zhou J, Green MR, Zheng X, Shen H. (2013)
hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene.
Apoptosis. 18(7):825-835.
 Constructs of wt or mutated FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. Synthesized sequences.In vivo splicing in HeLa, HCT-116, MDA-MB-231.In vivo splicing using wt and mutated sequences in MDA-MB-231, HeLa, HCT-116
hnRNP A1CAAAGAGGAA5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
23430061Oh HK, Lee E, Jang HN, Lee J, Moon H, Sheng Z, Jun Y, Loh TJ, Cho S, Zhou J, Green MR, Zheng X, Shen H. (2013)
hnRNP A1 contacts exon 5 to promote exon 6 inclusion of apoptotic Fas gene.
Apoptosis. 18(7):825-835.
 Constructs of wt or mutated FAS [355] EX5 - INT5 - EX6 - INT6 - EX7. Synthesized sequences.In vivo splicing in HeLa, HCT-116, MDA-MB-231.In vivo splicing using wt and mutated sequences in MDA-MB-231, HeLa, HCT-116
hnRNP A1AGAUAU-8Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A1AAUUUA-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A1AGUAGG-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A1UUCGAUUAGUGAA-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCCAUUAGUGAA-4Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGCUUAGUGAA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGACUAGUGAA-10Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGAUCAGUGAA-7Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGAUUCGUGAA-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGAUUACUGAA-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCCAUUACUGAA-3Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGCUUCGUGAA-1Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1UUCGACCAGUGAA-9Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24628426Rollins C, Levengood JD, Rife BD, Salemi M, Tolbert BS. (2014)
Thermodynamic and Phylogenetic Insights into hnRNP A1 Recognition of the HIV-1 Exon Splicing Silencer 3 Element.
Biochemistry. 53(13):2172-2184.
RNA secondary structure is essential for hnRNP A1 binding. In fact only the central tract of this motif is not sufficient. Sequences deriving from HIV-1 Tat [155871] Calorimetric titrations, saturation transfer difference NMR, chemical shift using wt and mutant sequences.
hnRNP A1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-5Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
24371143Zamiri B, Reddy K, Macgregor RB Jr, Pearson CE. (2014) TMPyP4 porphyrin distorts RNA G-quadruplex structures of the disease-associated r(GGGGCC)n repeat of the C9orf72 gene and blocks interaction of RNA-binding proteins. J Biol Chem. 289(8):4653-4659.  Synthetic sequences. EMSA with recombinant protein.
hnRNP A1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP A1GCAGUGACGACGCGCUGCUCAAGAACUACGGUCUGCUCUCCUGCUUCCGGAAGGACCUGCAUAAGACGGAGACGUACCUGAGGGUCAUGAAGUGCCGCCGCUUCGGGGAGGCCAG-2Gene Name and Synonymous: HNRNPA1, heterogeneous nuclear ribonucleoprotein A1, HNRPA1, MGC102835.
hnRNP A1 carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:8521471).
8276242Sun Q, Mayeda A, Hampson RK, Krainer AR, Rottman FM. (1993)
General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer.
Genes Dev. 7(12B):2598-2608.
 Construct of cow GH1 [280804] EX4 - INT4 - EX5In vitro splicing in HeLa nuclear extract, using also competing RNAs or protein excessUV crosslinking and immunoprecipitation in HeLa nuclear extract, UV crosslinking with purified HeLa protein or recombinant protein
hnRNP A2/B1GCCAAGGAGCC-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A2/B1AGAAC-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18503770Skoko N, Baralle M, Buratti E, Baralle FE. (2008)
The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors.
FEBS Lett. 582(15):2231-2236.
 Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37.In vivo splicing in HeLa, siRNA.UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts.
hnRNP A2/B1CUAGACUAGA-4Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
10406810Caputi M, Mayeda A, Krainer AR, Zahler AM. (1999)
hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing.
EMBO J. 18(14):4060-4067.
 Constructs and mutants of HIV-1 tat [155871] EX1 - INT1 - EX2In vitro splicing with HeLa nuclear extracts, nuclear extract depletion/reconsitution assay.RNA affinity chromatography, immunoblotting.
hnRNP A2/B1CAAGCACCGAACCCGCAACUG-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A2/B1GCGAAGGAGCC-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A2/B1GCCAAGAAGCC-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A2/B1GCCAAGGGGCC-2Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A2/B1GCCAAGGAACC-2Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
11024030Shan J, Moran-Jones K, Munro TP, Kidd GJ, Winzor DJ, Hoek KS, Smith R. (2000)
Binding of an RNA trafficking response element to heterogeneous nuclear ribonucleoproteins A1 and A2.
J Biol Chem. 275(49): 38286-38295.
 synthesized oligos UV crosslink, EMSA, IAsys resonant mirror biosensor.
hnRNP A2/B1GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
hnRNP A2/B1UAGACA-8Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12833158Kashima T, Manley JL. (2003)
A negative element in SMN2 exon 7 inhibits splicing in spinal muscular atrophy.
Nat Genet. 34(4): 460-463.
 Constructs and mutants of SMN1 [6606] and SMN2 [6607] EX7.In vivo splicing in HeLa.UV crosslink, SDS-PAGE, immunoprecipitation, siRNA and Western blot.
hnRNP A2/B1AUAGCA-9Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12426391Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002)
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
EMBO J. 21(22):6195-6204.
 Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells.RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting.
hnRNP A2/B1UUUAUA-7Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12426391Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002)
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
EMBO J. 21(22):6195-6204.
 Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells.RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting.
hnRNP A2/B1AUUUAA-2Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12426391Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG. (2002)
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
EMBO J. 21(22):6195-6204.
 Construct of murine Epb4.1 [269587] EX15 - INT15 - EX16 - INT16 - EX17In vitro splicing assay with recombinant protein. In vivo splicing assay in HeLa cells.RNA affinity isolation from HeLa nuclear extract using WT and mutated RNAs. Immunoblotting.
hnRNP A2/B1UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A2/B1UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A2/B1UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A2/B1UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A2/B1UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A2/B1UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP A2/B1CUAGUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1GAAGUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1CUUCUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1CUAGUACUAACUAAACUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1CUAGUAGAUUCUAAACUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1CUAGUAGAAACUAUUCUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1GAUCUAGAAACUAAACUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
12060656Hutchison S, LeBel C, Blanchette M, Chabot B. (2002)
Distinct sets of adjacent heterogeneous nuclear ribonucleoprotein (hnRNP) A1/A2 binding sites control 5' splice site selection in the hnRNP A1 mRNA precursor.
J Biol Chem. 277(33):29745-29752.
 Constructs of wt and mutated mouse Hnrnpa1 [15382] EX7 - INT7 - EX7B - INT7 - Ad_EXIn vitro splicing in HeLa NEEMSA with HeLa NE and recombinant protein. Depletion/reconstitution assay.
hnRNP A2/B1UAGGGAUAGGGUUAGGGA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
16157593Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005)
TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing.
J Biol Chem. 280(45):37572-37584.
 Synthesized sequences EMSA with recombinant protein
hnRNP A2/B1GCCAAGGAGCCAGAGAGCAUG-7Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
16548521Landsberg MJ, Moran-Jones K, Smith R. (2006)
Molecular recognition of an RNA trafficking element by heterogeneous nuclear ribonucleoprotein A2.
Biochemistry. 45(12):3943-3951.
 Synthesized sequences Pull-down assay, UV cross-linking, CD spectroscopy with recombinant protein
hnRNP A2/B1CAGCAUUAUGAAAG-10Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A2/B1CCGCAUUAUGAAAG-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A2/B1CAGCAUUAUGAACG-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A2/B1CCGCAUUAUGAACG-1Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18371932Hua Y, Vickers TA, Okunola HL, Bennett CF, Krainer AR. (2008)
Antisense masking of an hnRNP A1/A2 intronic splicing silencer corrects SMN2 splicing in transgenic mice.
Am J Hum Genet. 82(4):834-848.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vitro splicing in HeLa NE and in vivo splicing in HEK293.RNA-affinity chromatography, western blot with HeLa NE.
hnRNP A2/B1UGCAGAUGCUUAGUUUGUGU-8Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
hnRNP A2/B1UGCAGAUGGUUAGUUUGUGU-10Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
hnRNP A2/B1GUAUCCUUCCCUGGCCGUGAUAGUAGAGCAGCAGGGCCAGGGGGGUGACACACC-2Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP A2/B1CCAAGGAGCCAGAGAGCAUGG-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18480411Raju CS, Goritz C, Nord Y, Hermanson O, Lopez-Iglesias C, Visa N, Castelo-Branco G, Percipalle P. (2008)
In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences.
Mol Biol Cell. 19(7):3008-3019.
 Sequences deriving from mouse Mbp [17196]. Protein affinity purification and EMSA with HeLa NE, cytoplasmic or protein extracts
hnRNP A2/B1AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP A2/B1UUUAGUCAGCCUUAUAGCUAA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
hnRNP A2/B1AGAUAU-1Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A2/B1AAUUUA-5Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A2/B1AGUAGG-9Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP A2/B1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP A2/B1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1, HNRPA2B1, RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, DKFZp779B0244.
hnRNP A2 is involved in cytoplasmic RNA transport (PMID:11024030).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP A3CCAAGGAGCCAGAGAGCAUGG-5Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. 18480411Raju CS, Goritz C, Nord Y, Hermanson O, Lopez-Iglesias C, Visa N, Castelo-Branco G, Percipalle P. (2008)
In cultured oligodendrocytes the A/B-type hnRNP CBF-A accompanies MBP mRNA bound to mRNA trafficking sequences.
Mol Biol Cell. 19(7):3008-3019.
 Sequences deriving from mouse Mbp [17196]. Protein affinity purification and EMSA with HeLa NE, cytoplasmic or protein extracts
hnRNP A3AUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG-5Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. 18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP A3GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP A3AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: HNRNPA3, heterogeneous nuclear ribonucleoprotein A3, FBRNP, HNRPA3, D10S102, MGC138232, MGC142030, 2610510D13Rik. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP CAUUUA-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
8473331Hamilton BJ, Nagy E, Malter JS, Arrick BA, Rigby WF. (1993)
Association of heterogeneous nuclear ribonucleoprotein A1 and C proteins with reiterated AUUUA sequences.
J Biol Chem. 268(12): 8881-7.
 Partial sequence of colony stimulating factor 2 CSF2 [1437] 3'UTR. UV crosslink and immunoprecipitation with cytoplasmic lysates of lymphocytes.
hnRNP CAGUAUUUUUGUGGA-10Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
9649627Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998)
Oligonucleotide binding specificities of the hnRNP C protein tetramer.
Nucleic Acids Res. 26(14): 3410-3417.
hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. synthesized oligos Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions.
hnRNP CGGGGGGGGGGGGGGGGGGGG-10Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
9649627Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998)
Oligonucleotide binding specificities of the hnRNP C protein tetramer.
Nucleic Acids Res. 26(14): 3410-3417.
hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. synthesized oligos Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions.
hnRNP CAGUAGGGGGGUGGA-8Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
9649627Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998)
Oligonucleotide binding specificities of the hnRNP C protein tetramer.
Nucleic Acids Res. 26(14): 3410-3417.
hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. synthesized oligos Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions.
hnRNP CGAUCACUUGUGUCAACACAG-8Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
9649627Soltaninassab SR, McAfee JG, Shahied-Milam L, LeStourgeon WM. (1998)
Oligonucleotide binding specificities of the hnRNP C protein tetramer.
Nucleic Acids Res. 26(14): 3410-3417.
hnRNP C ha no affinity for polypyrimidine tracts of INT1 of the adenovirus-2 major late transcript, for adenovirus-2 oncoprotein E1A 3' splice site, for consensus polypyrimidine tract for U2AF65, for INT2 of human a-tropomyosin (PTB binding site), for (AUUUA)4 repeats and for r(U)20. synthesized oligos Affinity assessed by fluorescence spectroscopy of purified protein in competition binding assay under equilibrium conditions.
hnRNP CAUCGCUUCUCGGCCUUUUGGCUAAGAUCA-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
8798770Temsamani J, Pederson T. (1996)
The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle.
J Biol Chem. 271(40): 24922-24926.
hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. Sequences of U2 snRNA [6066] and homopolymers UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
hnRNP CUUUUUUU-7Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
8798770Temsamani J, Pederson T. (1996)
The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle.
J Biol Chem. 271(40): 24922-24926.
hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. Sequences of U2 snRNA [6066] and homopolymers UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
hnRNP CUGGAUUUUUUUCGGGUA-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
12509468Kim JH, Paek KY, Choi K, Kim TD, Hahm B, Kim KT, Jang SK. (2003)
Heterogeneous nuclear ribonucleoprotein C modulates translation of c-myc mRNA in a cell cycle phase-dependent manner.
Mol Cell Biol. 23(2):708-720.
 Synthesized sequences. UV cross-linking, Immunoprecipitation, deletion assay
hnRNP CGGGGGGG-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
12509468Kim JH, Paek KY, Choi K, Kim TD, Hahm B, Kim KT, Jang SK. (2003)
Heterogeneous nuclear ribonucleoprotein C modulates translation of c-myc mRNA in a cell cycle phase-dependent manner.
Mol Cell Biol. 23(2):708-720.
 Synthesized sequences. UV cross-linking, Immunoprecipitation, deletion assay
hnRNP CUCCCUUUUUUUUCCACAG-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP CUCUUUCCUUCUUUUUUCCUUUCUUUUCCUUCCUUCUUUAAU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
10037806Gontarek RR, Gutshall LL, Herold KM, Tsai J, Sathe GM, Mao J, Prescott C, Del Vecchio AM. (1999)
hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3'NTR of the HCV RNA genome.
Nucleic Acids Res. 27(6):1457-1463.
 HCV 3' NTR UV cross-linking, immunoprecipitation, competition assay with HepG2 cell extract
hnRNP CAUCGCUUCUCGGCCUUUUAAGAUUCUAGA-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
8798770Temsamani J, Pederson T. (1996)
The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle.
J Biol Chem. 271(40): 24922-24926.
hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. Sequences of U2 snRNA [6066] and homopolymers UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
hnRNP CAUCGCUUCUCGGCCAAAAGGCUAAGAUCA-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
8798770Temsamani J, Pederson T. (1996)
The C-group heterogeneous nuclear ribonucleoprotein proteins bind to the 5' stem-loop of the U2 small nuclear ribonucleoprotein particle.
J Biol Chem. 271(40): 24922-24926.
hnRNP C han no affinity to poly(A), poly(C) and U1 RNA. Stem contributes to the binding. Sequences of U2 snRNA [6066] and homopolymers UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
hnRNP CGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP CAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP CCCCUUUUUUUUCCACAG-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP CCCUUCUUCUUUUUCCUACAG-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2.
hnRNP C proteins are restricted to the nucleus because they bear a nuclear retention sequence (NRS), (PMID:8830767).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP C1UUUUUU-10Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
8083209Gorlach M, Burd CG, Dreyfuss G. (1994)
The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
J Biol Chem. 269(37): 23074-8.
hnRNP C1 has no affinity to poly(rC). Sequences of 20 nt random for SELEX. SELEX of 20nt random sequences with purified protein confirmed by filter binding assay.
hnRNP C1UUUUUA-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
8083209Gorlach M, Burd CG, Dreyfuss G. (1994)
The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
J Biol Chem. 269(37): 23074-8.
hnRNP C1 has no affinity to poly(rC). Sequences of 20 nt random for SELEX. SELEX of 20nt random sequences with purified protein confirmed by filter binding assay.
hnRNP C1UUUUUC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
8083209Gorlach M, Burd CG, Dreyfuss G. (1994)
The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
J Biol Chem. 269(37): 23074-8.
hnRNP C1 has no affinity to poly(rC). Sequences of 20 nt random for SELEX. SELEX of 20nt random sequences with purified protein confirmed by filter binding assay.
hnRNP C1UUUUUG-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
8083209Gorlach M, Burd CG, Dreyfuss G. (1994)
The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
J Biol Chem. 269(37): 23074-8.
hnRNP C1 has no affinity to poly(rC). Sequences of 20 nt random for SELEX. SELEX of 20nt random sequences with purified protein confirmed by filter binding assay.
hnRNP C1UUUUUUU-7Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP C1UUUUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
11172920Sokolowski M, Schwartz S. (2001)
Heterogeneous nuclear ribonucleoprotein C binds exclusively to the functionally important UUUUU-motifs in the human papillomavirus type-1 AU-rich inhibitory element.
Virus Res. 73(2):163-175
 Sequence of Papillomavirus HPV-1 AU-rich element (h1ARE). UV crosslink and SDS-PAGE with recombinant protein, competition assay.
hnRNP C1GGGGGGG-4Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
7688265Siomi H, Siomi MC, Nussbaum RL, Dreyfuss G. (1993)
The protein product of the fragile X gene, FMR1, has characteristics of an RNA-binding protein.
Cell. 74(2):291-298.
 Homopolymers Homopolymer binding assay with recombinant protein
hnRNP C1UGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
hnRNP C1UGAACUUUAUAGUUGUUUUAUAUGUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
hnRNP C1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP C1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
Isoform C1 is due to Alternative Splicing.
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP C2UUUUUUU-7Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP C2UUUUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
9393875Sokolowski M, Zhao C, Tan W, Schwartz S. (1997)
AU-rich mRNA instability elements on human papillomavirus type 1 late mRNAs and c-fos mRNAs interact with the same cellular factors.
Oncogene. 15(19):2303-2319.
hnRNP C1 and C2 do not bind AUUUA motifs. Sequences and mutants of 3' UTR HPV-1 late mRNA. EMSA, UV crosslink, Western blot and immunoprecipitation with HeLa nuclear extracts.
hnRNP C2GGAUAC-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP C2GCAUAC-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP C2GGAUUC-4Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP C2GGGUAC-7Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP C2GGAUAU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP C2UUUUUUUUUUUUUUUUUUUUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
16157593Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005)
TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing.
J Biol Chem. 280(45):37572-37584.
 Synthesized sequences EMSA with recombinant protein
hnRNP C2UGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
hnRNP C2UGAACUUUAUAGUUGUUUUAUAUGUU-5Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
hnRNP C2GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP C2AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPC, heterogeneous nuclear ribonucleoprotein C (C1/C2), C1, C2, HNRPC, SNRPC, MGC104306, MGC105117, MGC117353, MGC131677
isoform C2 is due to Alternative Splicing.
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP DAUUUA-1Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DUUAGGG-8Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP DUUAGGA-6Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP DUUAGAG-4Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP DCACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP DAUAAAAGAACUUUUUUAUGCUUACCAUCUUUUUUUUUUCUUUAACAGAUUUGUAUUUAAGAAUUGUUUUUAAAAAAUUUUAAGAUUUACACAAUGUUUCUCUGUAAAUA-9Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU-10Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DAUUUAUUUAUUUAUUUAUUUA-8Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DAUUUAUUUAUUUAUUUA-6Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DAUUUAUUUAUUUA-3Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DAUUUAUUUA-2Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 8647811DeMaria CT, Brewer G. (1996)
AUF1 binding affinity to A+U-rich elements correlates with rapid mRNA degradation.
J Biol Chem. 271(21): 12179-84.
hnRNP D has no affinity to poly(rU). WT and mutants rabbit beta-globin [100009084] 3'UTR, c-fos [2353] and c-myc [4609] UV crosslink and SDS-PAGE with recombinant protein. EMSA with recombinant protein.
hnRNP DGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAG-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 11514570Wilson GM, Sutphen K, Moutafis M, Sinha S, Brewer G. (2001)
Structural remodeling of an A + U-rich RNA element by cation or AUF1 binding.
J Biol Chem. 276(42):38400-38409.
 Synthesized sequences Resonance energy transfer (RET)
hnRNP DGAUCUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUA-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 11514570Wilson GM, Sutphen K, Moutafis M, Sinha S, Brewer G. (2001)
Structural remodeling of an A + U-rich RNA element by cation or AUF1 binding.
J Biol Chem. 276(42):38400-38409.
 Synthesized sequences Resonance energy transfer (RET)
hnRNP DUUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 11719186Chen CY, Gherzi R, Ong SE, Chan EL, Raijmakers R, Pruijn GJ, Stoecklin G, Moroni C, Mann M, Karin M. (2001)
AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.
Cell. 107(4):451-464.
 Sequences deriving from FOS [2353] UV crosslinking, imunoprecipitation with Jurkat extracts
hnRNP DUCAGCUAUUUACUGCCAAAGGGAAAUAUCAUUUAUUUUUUACAUUAUUAAGAAAAAAAGAUUUAUUUAUUUAAGACAGUCCCAUCAAAACUCCUGUCUUUGGAAAUC-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 11856759Lapucci A, Donnini M, Papucci L, Witort E, Tempestini A, Bevilacqua A, Nicolin A, Brewer G, Schiavone N, Capaccioli S. (2002)
AUF1 Is a bcl-2 A+U-rich element-binding protein involved in bcl-2 mRNA destabilization during apoptosis.
J Biol Chem. 277(18):16139-16146.
 Sequences deriving from BCL2 [596] EMSA supershift in Jurkat cytoplasmic extracts
hnRNP DGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAC-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 12819194Wilson GM, Lu J, Sutphen K, Suarez Y, Sinha S, Brewer B, Villanueva-Feliciano EC, Ysla RM, Charles S, Brewer G. (2003)
Phosphorylation of p40AUF1 regulates binding to A+U-rich mRNA-destabilizing elements and protein-induced changes in ribonucleoprotein structure.
J Biol Chem. 278(35):33039-33048.
 Sequences deriving from TNF [7124] EMSA with recombinant protein
hnRNP DUUUUUUAAAGUUUCUUGCAUUUAUUAUUCUCAAAAGUUUUUUCUAAGUUAAACAGUCAGUAUGCAAUCUUAAUAUAUGCUUUCUUUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 16289864Sommer S, Cui Y, Brewer G, Fuqua SA. (2005)
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
J Steroid Biochem Mol Biol. 97(3):219-229.
 Sequences deriving from YES1 [7525] EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts.
hnRNP DUUUUUAUGUAAAACAUUUUUAGAACUCCAGUUUUCAAAUCAUGUUUGAAUCUACAUUCACUUUUUUUUGUUUUCUUUUUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 16289864Sommer S, Cui Y, Brewer G, Fuqua SA. (2005)
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
J Steroid Biochem Mol Biol. 97(3):219-229.
 Sequences deriving from YES1 [7525] EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts.
hnRNP DUGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
hnRNP DUGAACUUUAUAGUUGUUUUAUAUGUU-10Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
hnRNP DAAAAAAA-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 16834569Sagliocco F, Laloo B, Cosson B, Laborde L, Castroviejo M, Rosenbaum J, Ripoche J, Grosset C. (2006)
The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP.
Biochem J. 400(2):337-347.
 Synthesized oligos Homopolymer binding assay and Western blot with HeLa extracts or recombinant protein.
hnRNP DAGACUUUAUGUAGUUUUUAUAUGUUGUAAUAUUUCUUCAAAUAAAUCUCUCCUAUAA-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 17030625Torrisani J, Unterberger A, Tendulkar SR, Shikimi K, Szyf M. (2007)
AUF1 cell cycle variations define genomic DNA methylation by regulation of DNMT1 mRNA stability.
Mol Cell Biol. 27(1):395-410.
 Sequences deriving from DNMT1 [1786]. Synthesized sequences. UV cross-linking in HeLa cytoplasmic extracts, MALDI-TOF, Western blot
hnRNP DCAUAAAUUAUUUUCAAGUGUAACUUAUUAACCUAUUUAUUAUUUAUGUAUUUAUUUAAGC-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 18650551Palanisamy V, Park NJ, Wang J, Wong DT. (2008)
AUF1 and HuR proteins stabilize interleukin-8 mRNA in human saliva.
J Dent Res. 87(8):772-776.
 Sequences deriving from IL8 [3576] UV cross-linking, immunoprecipitation with salivary protein extracts.
hnRNP DCCAUUUAUAUCAUUUUUUAUAUAUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
hnRNP DCCAUUUAUAUCAUAAAAUAUAUAUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
hnRNP DCCAUUUAUAUCAUUUUUAAAAAAUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
hnRNP DCCAUUUAUAUCAUAAAAAUAUUAUU-5Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
hnRNP DAGAUAU-9Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP DAAUUUA-9Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP DAGUAGG-9Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP D0UUAGGG-6Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD
Isoform:Isoform D0 is due to Alternative Splicing.
7673195Kajita Y, Nakayama J, Aizawa M, Ishikawa F. (1995)
The UUAG-specific RNA binding protein, heterogeneous nuclear ribonucleoprotein D0. Common modular structure and binding properties of the 2xRBD-Gly family.
J Biol Chem. 270(38): 22167-22175.
 Synthesized sequences Filter Binding Assay of recombinant protein and synthesized oligos.
hnRNP D0GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD
Isoform:Isoform D0 is due to Alternative Splicing.
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP D0AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPD, heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa), P37, AUF1, AUF1A, HNRPD
Isoform:Isoform D0 is due to Alternative Splicing.
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP DLAACCUUGCC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLAAUACCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACACCAGAC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACAUUAGCC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACCACGCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUACAGCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAACU-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAAGUA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAGAG-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAGCC-10Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAGCG-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAGCU-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUAGGA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUGCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLACUUUAA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLAGUAGCC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLAUCUGAC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLAUGCGCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLAUUAGGCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLCCUUUAGGC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGAACUAAGC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGACUAGCA-5Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGACUAGCG-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGCACUAGAU-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGCACUAGGC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGCGAGCA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGCUAGUA-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGGACUAGCC-5Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGGACUAGCU-3Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGGAGUAGCC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGGAGUAGCU-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLGGAUUAGCC-7Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLUGUCGC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 12406575Kamei D, Yamada M. (2003)
Interactions of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its domains with high-affinity binding sites.
Gene 298(1):49-57.
 Sequences of 20 nt random for SELEX. SELEX of 20nt random with protein and filter binding assay. UV crosslink, immunoblot on HL-60 cells.
hnRNP DLUUUAGUCAGCCUUAUAGCUAA-5Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
hnRNP DLAGAUAU-7Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP DLAAUUUA-9Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP DLAGUAGG-9Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 24452013Choudhury R, Roy SG, Tsai YS, Tripathy A, Graves LM, Wang Z. (2014)
The splicing activator DAZAP1 integrates splicing control into MEK/Erk-regulated cell proliferation and migration.
Nat Commun. 5:3078.
 Synthetic sequences. Construct of Chinese hamster Dhfr [100689028] GFP - INT1 - EX2 - INT2 - GFP where INT2 contains a MS2 hairpin.In vivo splicing in HEK-293T using MS2-DAZAP1 fusion proteins.RNA affinity chromatography and SPR analysis with recombinant protein.
hnRNP DLGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP DLAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRPDL, heterogeneous nuclear ribonucleoprotein D-like, JKTBP, JKTBP2, laAUF1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP E1UUAGGG-8Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP E1UUAGGA-6Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP E1UUAGAG-4Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP E1CCCCCCC-7Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 7607214Leffers H, Dejgaard K, Celis JE. (1995)
Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.
Eur J Biochem. 230(2): 447-453.
hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). homopolymers Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers.
hnRNP E1GGGGGGG-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP E1UUUUUUU-4Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 7607214Leffers H, Dejgaard K, Celis JE. (1995)
Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.
Eur J Biochem. 230(2): 447-453.
hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). homopolymers Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers.
hnRNP E1UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP E1UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU-2Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP E1CCCAACGGGCCCUCCUCCCC-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 9122208Holcik M, Liebhaber SA. (1997)
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Proc Natl Acad Sci U S A. 94(6):2410-2414.
 Synthesized sequences. RNase H mapping, EMSA, immunoblot
hnRNP E1CCCAGCCGGCCCUCCUCCCC-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 9122208Holcik M, Liebhaber SA. (1997)
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Proc Natl Acad Sci U S A. 94(6):2410-2414.
 Synthesized sequences. RNase H mapping, EMSA, immunoblot
hnRNP E1CCCCACGGGCCCUCCUCCCC-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 9122208Holcik M, Liebhaber SA. (1997)
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Proc Natl Acad Sci U S A. 94(6):2410-2414.
 Synthesized sequences. RNase H mapping, EMSA, immunoblot
hnRNP E1CCCCACCCUCUUCCCCAAGCCCCACCCUCUUCCCCAAG-4Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 9160751Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M, Hentze MW. (1997)
mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end.
Cell. 16
 Constructs of luciferase gene reporter.In vivo splicing assay with luciferase gene reporter in HeLaUV cross-linking. Cell-free translation of different mRNAs.
hnRNP E1CCCUCCC-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 12011088Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002)
Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.
J Biol Chem. 277(30):27183-27192.
 Sequences deriving from AR [367]. Synthesized oligos. Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts
hnRNP E1CCCUCUGCCACCCAGGCAGGCCCUGCCUUCAGCCCUGGCCCAGAGCUGGAACACUCUCUGAGAUGCCCCUCUGCCUGGGCUUAUGCCCUCAGAUGGAGACAUUGGAUGUGGAGCUCCUGCUGGAUGCGUGCCCUGACCCCUGCACCAGCCCUUCCCUGCUUUGAG-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 12600897Skalweit A, Doller A, Huth A, Kahne T, Persson PB, Thiele BJ. (2003)
Posttranscriptional control of renin synthesis: identification of proteins interacting with renin mRNA 3'-untranslated region.
Circ Res. 92(4):419-427.
 Sequences deriving from REN [5972] EMSA, UV cross-linking with Calu-6 cell extracts. RNA-affinity chromatography with MALDI-TOF-MS. Western blot.
hnRNP E1CCCAACCUGGCUCCCUCCCACCCAACCAACUUUCCCCCCAACCC-10Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 15514164Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004)
RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis.
Circ Res. 95(11):1058-1066.
 Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF.
hnRNP E1CUCCUUCCCCCGCUCCCCCAA-4Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 15514164Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004)
RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis.
Circ Res. 95(11):1058-1066.
 Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF.
hnRNP E1GCCGCUCCAGCGCCGCGCAGCCACCGCCGCCGC-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 15967798Thomson AM, Cahill CM, Cho HH, Kassachau KD, Epis MR, Bridges KR, Leedman PJ, Rogers JT. (2005)
The acute box cis-element in human heavy ferritin mRNA 5'-untranslated region is a unique translation enhancer that binds poly(C)-binding proteins.
J Biol Chem. 280(34):30032-30045.
 Sequences deriving from FTH1 [2495] EMSA, UV cross-linking, western blot with HepG2 cytoplasmic extract or recombinant protein
hnRNP E1UCCCCAA-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 16854432Messias AC, Harnisch C, Ostareck-Lederer A, Sattler M, Ostareck DH. (2006)
The DICE-binding activity of KH domain 3 of hnRNP K is affected by c-Src-mediated tyrosine phosphorylation.
J Mol Biol. 361(3):470-481.
 Synthesized sequences UV cross-linking, Chemical shift mapping (NMR) with recombinant protein.
hnRNP E1AAAAAAA-2Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 8917439Dejgaard K, Leffers H. (1996)
Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains.
Eur J Biochem. 241(2):425-431.
 Synthesized oligos Homopolymer binding assay with recombinant protein.
hnRNP E1CCCUUCCC-10Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1AAAUUCCC-10Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1CCCUUAAA-10Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1ACAUUAAA-1Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1CAAUUAAA-2Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1CCAUUAAA-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1ACCUUAAA-7Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1AAACCAAA-9Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1AAAUUCCA-10Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1AAAUUACC-9Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1AAAUUCAC-3Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1CUCUGGGGUUGUACCCACCCCAGAG-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E1AGCCACCUCCCACCCCACCCCA-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17928403Lee PT, Liao PC, Chang WC, Tseng JT. (2007)
Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover.
Mol Biol Cell. 18(12):5004-5013.
 Sequences deriving from mutated GAST [2520] and homopolymers. EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts.
hnRNP E1CCCAGCCCUGUCCCCUGAAAAACUGA-5Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 17928403Lee PT, Liao PC, Chang WC, Tseng JT. (2007)
Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover.
Mol Biol Cell. 18(12):5004-5013.
 Sequences deriving from mutated GAST [2520] and homopolymers. EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts.
hnRNP E1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP E1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: PCBP1, poly(rC) binding protein 1, HNRPX, HNRPE1, hnRNP-X, hnRNP-E1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP E2UUAGGG-8Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP E2UUAGGA-6Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP E2UUAGAG-4Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 8321232Ishikawa F, Matunis MJ, Dreyfuss G, Cech TR. (1993)
Nuclear proteins that bind the pre-mRNA 3' splice site sequence r(UUAG/G) and the human telomeric DNA sequence d(TTAGGG)n.
Mol Cell Biol. 13(7): 4301-4310.
 synthesized oligos EMSA with Hela nuclear extract and synthesized oligos. EMSA with purified proteins and synthesized oligos.
hnRNP E2CCCCCCC-7Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 7607214Leffers H, Dejgaard K, Celis JE. (1995)
Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.
Eur J Biochem. 230(2): 447-453.
hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). homopolymers Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers.
hnRNP E2GGGGGGG-7Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP E2UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP E2UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU-2Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP E2CCCAACGGGCCCUCCUCCCC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 9122208Holcik M, Liebhaber SA. (1997)
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Proc Natl Acad Sci U S A. 94(6):2410-2414.
 Synthesized sequences. RNase H mapping, EMSA, immunoblot
hnRNP E2CCCAGCCGGCCCUCCUCCCC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 9122208Holcik M, Liebhaber SA. (1997)
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Proc Natl Acad Sci U S A. 94(6):2410-2414.
 Synthesized sequences. RNase H mapping, EMSA, immunoblot
hnRNP E2CCCCACGGGCCCUCCUCCCC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 9122208Holcik M, Liebhaber SA. (1997)
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components.
Proc Natl Acad Sci U S A. 94(6):2410-2414.
 Synthesized sequences. RNase H mapping, EMSA, immunoblot
hnRNP E2CUCGCCAUGCCGGGAGAACUCUAACUCCCCCAUGGAG-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 11753385Perrotti D, Cesi V, Trotta R, Guerzoni C, Santilli G, Campbell K, Iervolino A, Condorelli F, Gambacorti-Passerini C, Caligiuri MA, Calabretta B. (2002)
BCR-ABL suppresses C/EBPalpha expression through inhibitory action of hnRNP E2.
Nat Genet. 30(1):48-58.
 Sequences deriving from CEBPA [1050] RNA affinity chromatography and EMSA supershift in K562 cytoplasmic extracts. Protein sequencing.
hnRNP E2CCCUCCC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 12011088Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002)
Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.
J Biol Chem. 277(30):27183-27192.
 Sequences deriving from AR [367]. Synthesized oligos. Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts
hnRNP E2UUUUUUU-7Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 12011088Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002)
Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.
J Biol Chem. 277(30):27183-27192.
 Sequences deriving from AR [367]. Synthesized oligos. Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts
hnRNP E2UCCCCA-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 15331611Du Z, Yu J, Chen Y, Andino R, James TL. (2004)
Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2.
J Biol Chem. 279(46):48126-48134.
 Synthesized sequences and HPV-1 IRES. NMR using recombinant protein
hnRNP E2CUAACCCUAA-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 15331611Du Z, Yu J, Chen Y, Andino R, James TL. (2004)
Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2.
J Biol Chem. 279(46):48126-48134.
 Synthesized sequences and HPV-1 IRES. NMR using recombinant protein
hnRNP E2GGAGGACAUCCCUCACGGGUGACCGUGGUCCUCC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 15331611Du Z, Yu J, Chen Y, Andino R, James TL. (2004)
Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2.
J Biol Chem. 279(46):48126-48134.
 Synthesized sequences and HPV-1 IRES. NMR using recombinant protein
hnRNP E2GGGGUUGUACCCACCCCAC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 15331611Du Z, Yu J, Chen Y, Andino R, James TL. (2004)
Specific recognition of the C-rich strand of human telomeric DNA and the RNA template of human telomerase by the first KH domain of human poly(C)-binding protein-2.
J Biol Chem. 279(46):48126-48134.
 Synthesized sequences and HPV-1 IRES. NMR using recombinant protein
hnRNP E2GCCGCUCCAGCGCCGCGCAGCCACCGCCGCCGC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 15967798Thomson AM, Cahill CM, Cho HH, Kassachau KD, Epis MR, Bridges KR, Leedman PJ, Rogers JT. (2005)
The acute box cis-element in human heavy ferritin mRNA 5'-untranslated region is a unique translation enhancer that binds poly(C)-binding proteins.
J Biol Chem. 280(34):30032-30045.
 Sequences deriving from FTH1 [2495] EMSA, UV cross-linking, western blot with HepG2 cytoplasmic extract or recombinant protein
hnRNP E2CCAUUC-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17451601Woolaway K, Asai K, Emili A, Cochrane A. (2007)
hnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expression.
Retrovirology. 4:28.
 Sequences deriving from HIV-1 Tat [155871]  Protein affinity purification with HeLa NE, mass spectrometry, western blot.
hnRNP E2CCCUUCCC-10Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2AAAUUCCC-10Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2CCCUUAAA-10Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2ACAUUAAA-1Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2CAAUUAAA-2Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2CCAUUAAA-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2ACCUUAAA-7Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2AAACCAAA-9Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2AAAUUCCA-10Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2AAAUUACC-9Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2AAAUUCAC-3Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2CUCUGGGGUUGUACCCACCCCAGAG-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 17609276Toyoda H, Franco D, Fujita K, Paul AV, Wimmer E. (2007)
Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site.
J Virol. 81(18):10017-10028.
 Sequences deriving from Poliovirus 1 (PV1) 5' nontranslated region. Pull-down assay with recombinant protein and HeLa cell extracts.
hnRNP E2CGCUCAGCACUACCCCAGUUGAGCU-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 18929541Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Gorlach M. (2008)
Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA.
Biochem Biophys Res Commun. 377(2):500-503.
 Sequences deriving from CVB3 5' UTR EMSA with recombinant protein
hnRNP E2CAGGUCGAUGAGUCACCGCAUUCCCCACGGGCGACCGUGGCGGUGGCUGCGUUGGCGGCCUG-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 18929541Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Gorlach M. (2008)
Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA.
Biochem Biophys Res Commun. 377(2):500-503.
 Sequences deriving from CVB3 5' UTR EMSA with recombinant protein
hnRNP E2CCUGUGGGUUGAUCCCACCCACAGG-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 18929541Zell R, Ihle Y, Effenberger M, Seitz S, Wutzler P, Gorlach M. (2008)
Interaction of poly(rC)-binding protein 2 domains KH1 and KH3 with coxsackievirus RNA.
Biochem Biophys Res Commun. 377(2):500-503.
 Sequences deriving from CVB3 5' UTR EMSA with recombinant protein
hnRNP E2CUCUGCCCUUCCGU-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 20211135Eiring AM, Harb JG, Neviani P, Garton C, Oaks JJ, Spizzo R, Liu S, Schwind S, Santhanam R, Hickey CJ, Becker H, Chandler JC, Andino R, Cortes J, Hokland P, Huettner CS, Bhatia R, Roy DC, Liebhaber SA, Caligiuri MA, Marcucci G, Garzon R, Croce CM, Calin GA, Perrotti D. (2010)
miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts.
Cell. 140(5):652-665.
 Sequences deriving from MIR328 [442901] EMSA, UV cross-linking and immunoprecipitation with human recombinant protein expressed in murine cells.
hnRNP E2CACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP E2GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP E2AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: PCBP2, poly(rC) binding protein 2, HNRPE2, hnRNP-E2, MGC110998. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP FGGGGGGG-7Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 7512260Matunis MJ, Xing J, Dreyfuss G. (1994)
The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.
Nucleic Acids Res. 22(6): 1059-67.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP FGGGGGCUG-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
hnRNP FGGAGGA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP FAAGGGGAA5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP FAGGGA-8Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP FCGGGC-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAAGGUG-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP FGGGGGAGGUGUGGG-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FAGGGGAGGUGUGGG-6Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGAGGGAGGUGUGGG-3Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGAGGAGGUGUGGG-1Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGAGAGGUGUGGG-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGAAGGUGUGGG-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAAGUGUGGG-3Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGAUGUGGG-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGGUAUGGG-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGGUGUAGG-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGGUGUGAG-10Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGGUGUGGA-7Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGGUGUGAA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAGGUGUAAG-3Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGGGAAAUGUGGG-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGGAAAGGUGUGGG-3Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGGAAGAGGUGUGGG-1Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FGAAGGAGGUGUGGG-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FAAGGGAGGUGUGGG-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP FCGAUGGGAA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FCGGGAUGGGGUA-10Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FCUGGGGU-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FUGGGA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FUGGGGU-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 12228232Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002)
Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene.
J Biol Chem. 277(46):43821-43829.
 Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9In vivo splicing in HeLaMutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting
hnRNP FGGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 11421362Hastings ML, Wilson CM, Munroe SH. (2001)
A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.
RNA. 7(6):859-874.
 Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing with HeLa NEUV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins.
hnRNP FAGGGUUGAGGGGAGCAGGGU-7Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP FCGGGUUGACGGGAGCACGGU-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP FAGUGUUGAGUGGAGCAGUGGU-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP FUGGGC-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGC-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGU-6Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGG-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FGGGGG-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FCGGGGGGGGC-6Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGGAGGGG-6Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGAAGGGA-8Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FAGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FCGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FCAGAAGGGGAGGGGUUCCA-10Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP FAACAAGGGGUGGGGGAAAA-10Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP FGGGGAAUAUACGUGCUUGGCGGGUAAUUCUAUUGGGA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 18573884Mauger DM, Lin C, Garcia-Blanco MA. (2008)
hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc.
Mol Cell Biol. 28(17):5403-5419.
 Constructs of FGFR2 [2263] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10.In vivo splicing in HEK293Splicing assays with wt and mutant minigenes. siRNA.
hnRNP FGGGAAUGUGGG-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 19506027Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009)
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.
Nucleic Acids Res. 37(14):4672-4683.
 Sequences deriving from HBB [3043] UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein
hnRNP FAAGGCGAA1Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP FCGGGA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP FGGGGA-4Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FUGGGG-3Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FGGGGU-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 16885237Dominguez C, Allain FH. (2006)
NMR structure of the three quasi RNA recognition motifs (qRRMs) of human hnRNP F and interaction studies with Bcl-x G-tract RNA: a novel mode of RNA recognition.
Nucleic Acids Res. 34(13):3634-3645.
 Synthesized sequences. NMR titration using recombinant protein
hnRNP FGGGGC-2Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 12228232Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002)
Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene.
J Biol Chem. 277(46):43821-43829.
 Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9In vivo splicing in HeLaMutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting
hnRNP FAGGGAU-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 20526337Dominguez C, Fisette JF, Chabot B, Allain FH. (2010)
Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs.
Nat Struct Mol Biol. 17(7):853-861.
 Synthesized sequences. Constructs of wt and mutant BCL2L1 [598] EX2 - INT2 - EX3.In vitro splicing assays in HeLa nuclear extracts.NMR spectroscopy. In vitro splicing assays in HeLa nuclear extracts with increasing amount of splicing factor.
hnRNP FGGGAUGGGGUAAACUGGGG5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 20526337Dominguez C, Fisette JF, Chabot B, Allain FH. (2010)
Structural basis of G-tract recognition and encaging by hnRNP F quasi-RRMs.
Nat Struct Mol Biol. 17(7):853-861.
 Synthesized sequences. Constructs of wt and mutant BCL2L1 [598] EX2 - INT2 - EX3.In vitro splicing assays in HeLa nuclear extracts.NMR spectroscopy. In vitro splicing assays in HeLa nuclear extracts with increasing amount of splicing factor.
hnRNP FGGAGGGUUAGGGUUAGGGUUAGGGUUA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 23275549Samatanga B, Dominguez C, Jelesarov I, Allain FH. (2013)
The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA.
Nucleic Acids Res. 41(4):2505-2516.
 Synthesized sequences NMR spectroscopy, isothermal titration calorimetry
hnRNP FGAGUUAGGGUUAGGGUUAGGGUUAGGGUUA-5Gene Name and Synonymous: HNRNPF, heterogeneous nuclear ribonucleoprotein F, HNRPF, mcs94-1, MGC110997, OK/SW-cl.23. 23275549Samatanga B, Dominguez C, Jelesarov I, Allain FH. (2013)
The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA.
Nucleic Acids Res. 41(4):2505-2516.
 Synthesized sequences NMR spectroscopy, isothermal titration calorimetry
hnRNP GGGGUACGACGGAUAUCGUGGGGGGGGAAAUUGCUUUCGGUUCCGACUCUG-5Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 21327109Kanhoush R, Beenders B, Perrin C, Moreau J, Bellini M, Penrad-Mobayed M. (2010)
Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts.
Nucleus. 1(1):109-122.
 Synthesized sequences 1D- and 2D-Northwestern assays, Westernblot with HeLa NE
hnRNP GAUCAAA5Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GUAAGAC5Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GACCAAA5Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GUCAAAA5Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GAAAAU5Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GAUCCCA1Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GAUCCCC1Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
hnRNP GGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP GAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: RBMX, RNA binding motif protein X-linked, RNMX, HNRPG, RBMXP1, RBMXRT, hnRNP-G. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP H1CGGGA-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
hnRNP H1AUUGGGUGU-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11526107Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001)
A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H.
J Biol Chem. 276(44): 40464-75.
 Constructs of HIV-1 Tat [155871] EX2.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.EMSA, UV crosslink, immunoprecipitation and immunoblot.
hnRNP H1GGGGGGG-7Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 7512260Matunis MJ, Xing J, Dreyfuss G. (1994)
The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.
Nucleic Acids Res. 22(6): 1059-67.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP H1GGGGGCUG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
hnRNP H1UGUGGG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 10072387Chen CD, Kobayashi R, Helfman DM. (1999)
Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene.
Genes Dev. 13(5):593-606.
 Construct of rat beta-tropomyosin Tpm2 [500450] EX5-INT5-EX7In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assays, immunoblot, immunodepletion in HeLa nuclear extracts.
hnRNP H1GGAGGA-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP H1CGAAUCGACAAAGGGGAGGAAGUGGGAGAAA-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 10934202Fogel BL, McNally MT. (2000)
A cellular protein, hnRNP H, binds to the negative regulator of splicing element from Rous sarcoma virus.
J Biol Chem. 275(41):32371-32378.
 Sequence and variants of NRS5' region Rous sarcoma virus. UV crosslink in HeLa nuclear extracts. RNA affinity selection with HeLa extracts. Immunoprecipitation, EMSA supershift.
hnRNP H1AAGGGGAA5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP H1AGGGA-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP H1CGGGC-6Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AUCGGGCGU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11526107Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001)
A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H.
J Biol Chem. 276(44): 40464-75.
 Constructs of HIV-1 Tat [155871] EX2.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.EMSA, UV crosslink, immunoprecipitation and immunoblot.
hnRNP H1AAGGUG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP H1UGGGC-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1UGGGG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H1GAUCACUGGGGUGGAUCAUCCAGGUGGGGCUUUU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16396608Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006)
Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.
PLoS Biol. 4(2):e21.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEImmunoprecipitation, Gel shift and RNase H protection assays
hnRNP H1GGGUCGAGGGG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16396608Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006)
Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.
PLoS Biol. 4(2):e21.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEImmunoprecipitation, Gel shift and RNase H protection assays
hnRNP H1GAUCACUGGGGUGGAUCAU-1Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H1GAUCACUGUGGUGGAUCAUCCAGGUGUGGCUUUU-1Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H1GAUCACUGUGGUGGAUCAUCCAGGUGGGGCUUUU-1Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H1CCAUGGUUUGGGAGUGGGAAGGUGGGGAG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H1CCAUGGUUUGGGGGCAGUAGUUGG-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H1UGGGGU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 12228232Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002)
Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene.
J Biol Chem. 277(46):43821-43829.
 Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9In vivo splicing in HeLaMutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting
hnRNP H1GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC-10Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11421362Hastings ML, Wilson CM, Munroe SH. (2001)
A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.
RNA. 7(6):859-874.
 Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing with HeLa NEUV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins.
hnRNP H1AGGGUUGAGGGGAGCAGGGU-7Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H1CGGGUUGACGGGAGCACGGU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H1AGUGUUGAGUGGAGCAGUGGU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H1GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP H1AUACCUUUUUGGGGAGGGGGCAGAGAGC-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16254078Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005)
Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene.
Nucleic Acids Res. 33(18):6000-6010.
 Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3In vivo splicing in Hep3BPull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA.
hnRNP H1AAGAA-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1CAGGAC-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGAGA-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGAGG-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGAG-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGC-6Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGU-6Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGG-6Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1GGGGG-6Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1CGGGGGGGGC-6Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGGAGGGG-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGAAGGGA-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1CAGAAGGGGAGGGGUUCCA-3Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP H1AACAAGGGGUGGGGGAAAA-3Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP H1GUAUCCUUCCCUGGCGGGGGUGGGAGAGCAGCAGGGCCAGGGGGGUGACACACC-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGGGAUGGGCGAGAUGGGGUGCUGU-10Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGGGAUGGGCGAGAUGGGGUGCUGU-4Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGUGAUGUGCGAGAUGGUGUGCUGU-8Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGUGAUGUGCGAGAUGGUGUGCUGU-1Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1GGUUGGGAGAGGGAUUUC-10Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1GGUUGUGAGAGUGAUUUC-1Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H1GGGGAAUAUACGUGCUUGGCGGGUAAUUCUAUUGGGA-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 18573884Mauger DM, Lin C, Garcia-Blanco MA. (2008)
hnRNP H and hnRNP F complex with Fox2 to silence fibroblast growth factor receptor 2 exon IIIc.
Mol Cell Biol. 28(17):5403-5419.
 Constructs of FGFR2 [2263] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10.In vivo splicing in HEK293Splicing assays with wt and mutant minigenes. siRNA.
hnRNP H1GGGAAUGUGGG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 19506027Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009)
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.
Nucleic Acids Res. 37(14):4672-4683.
 Sequences deriving from HBB [3043] UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein
hnRNP H1GGGGU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H1UGGGA-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H1UGGGU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 20100605Russo A, Siciliano G, Catillo M, Giangrande C, Amoresano A, Pucci P, Pietropaolo C, Russo G. (2010)
hnRNP H1 and intronic G runs in the splicing control of the human rpL3 gene.
Biochim Biophys Acta. 1799(5-6):419-428.
 Construct of RPL3 [6122] EX3 - INT3 - EX4In vivo splicing in HeLaGST pull-down, mass spectrometry, Immunoprecipitation, western blotting, RNA pull-down assay, REMSA, RNA interference in HeLa and Calu6
hnRNP H1CUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1CUGAGGCUGGGGGCUGGAAUUCCCAUGUGCUUCCU-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1UAUGACCCGGACUCCCGGUG-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1UAUGAUAGGCACUUAGGCUG-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1CUGGGGGCUG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1GGGGC-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1AAGGCGAA1Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP H1CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H1GGGGA-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 24290757Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V, Miller JW, Smith BN, Gallo JM, Ule J, Hirth F, Rogelj B, Houart C, Shaw CE. (2013)
Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic.
Cell Rep. 5(5):1178-1186.
 Synthetic sequences. RNA pull-down assay using SH-SY5Y cell extract.
hnRNP H1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP H1UGGGACUGGGACUGGGACUG-5Gene Name and Synonymous: HNRNPH1, heterogeneous nuclear ribonucleoprotein H1 (H), HNRPH, HNRPH1, hnRNPH, DKFZp686A15170. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP H2CGGGA-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
hnRNP H2AUUGGGUGU-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11526107Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001)
A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H.
J Biol Chem. 276(44): 40464-75.
 Constructs of HIV-1 Tat [155871] EX2.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.EMSA, UV crosslink, immunoprecipitation and immunoblot.
hnRNP H2GGGGGGG-7Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 7512260Matunis MJ, Xing J, Dreyfuss G. (1994)
The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.
Nucleic Acids Res. 22(6): 1059-67.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP H2GGGGGCUG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
hnRNP H2UGUGGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 10072387Chen CD, Kobayashi R, Helfman DM. (1999)
Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene.
Genes Dev. 13(5):593-606.
 Construct of rat beta-tropomyosin Tpm2 [500450] EX5-INT5-EX7In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assays, immunoblot, immunodepletion in HeLa nuclear extracts.
hnRNP H2GGAGGA-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP H2CGAAUCGACAAAGGGGAGGAAGUGGGAGAAA-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 10934202Fogel BL, McNally MT. (2000)
A cellular protein, hnRNP H, binds to the negative regulator of splicing element from Rous sarcoma virus.
J Biol Chem. 275(41):32371-32378.
 Sequence and variants of NRS5' region Rous sarcoma virus. UV crosslink in HeLa nuclear extracts. RNA affinity selection with HeLa extracts. Immunoprecipitation, EMSA supershift.
hnRNP H2AAGGGGAA5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP H2AGGGA-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19404736Millevoi S, Bernat S, Telly D, Fouque F, Gladieff L, Favre G, Vagner S, Toulas C. (2009)
The c.5242C>A BRCA1 missense variant induces exon skipping by increasing splicing repressors binding.
Breast Cancer Res Treat. 120(2):391-399.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19. WT or mutated exon 18 RNA substrates.In vitro splicing in HeLa nuclear extractMutational analysis, RNA affinity chromatography, UV cross-linking, immunoprecipitation with HeLa nuclear extract.
hnRNP H2CGGGC-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AUCGGGCGU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11526107Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001)
A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H.
J Biol Chem. 276(44): 40464-75.
 Constructs of HIV-1 Tat [155871] EX2.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.EMSA, UV crosslink, immunoprecipitation and immunoblot.
hnRNP H2AAGGUG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP H2UGGGC-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2UGGGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H2GGGGGAGGUGUGGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2AGGGGAGGUGUGGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GAGGGAGGUGUGGG-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGAGGAGGUGUGGG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGAGAGGUGUGGG-4Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGAAGGUGUGGG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAAGUGUGGG-3Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGAUGUGGG-4Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGGUAUGGG-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGGUGUAGG-4Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGGUGUGAG-10Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGGUGUGGA-10Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGGUGUGAA-4Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAGGUGUAAG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGGGAAAUGUGGG-7Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGGAAAGGUGUGGG-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGAAGAGGUGUGGG-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GAAGGAGGUGUGGG-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2AAGGGAGGUGUGGG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GGAGAAGGUGUGGG-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16171461Alkan SA, Martincic K, Milcarek C. (2006)
The hnRNPs F and H2 bind to similar sequences to influence gene expression.
Biochem J. 393(Pt 1):361-371.
 SVL (SV40 late) pre-mRNA and mutants UV cross-linking, EMSA, filter binding assays using recombinant protein
hnRNP H2GAUCACUGGGGUGGAUCAUCCAGGUGGGGCUUUU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16396608Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006)
Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.
PLoS Biol. 4(2):e21.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEImmunoprecipitation, Gel shift and RNase H protection assays
hnRNP H2GGGUCGAGGGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16396608Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006)
Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.
PLoS Biol. 4(2):e21.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEImmunoprecipitation, Gel shift and RNase H protection assays
hnRNP H2GAUCACUGGGGUGGAUCAU-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H2GAUCACUGUGGUGGAUCAUCCAGGUGUGGCUUUU-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H2GAUCACUGUGGUGGAUCAUCCAGGUGGGGCUUUU-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H2CCAUGGUUUGGGAGUGGGAAGGUGGGGAG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H2CCAUGGUUUGGGGGCAGUAGUUGG-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H2UGGGGU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 12228232Romano M, Marcucci R, Buratti E, Ayala YM, Sebastio G, Baralle FE. (2002)
Regulation of 3' splice site selection in the 844ins68 polymorphism of the cystathionine beta-synthase gene.
J Biol Chem. 277(46):43821-43829.
 Construct of CBS [875] EX5 - INT5 - EX6 - INT6 - EX7 - INT7 - EX8 - INT8 - EX9In vivo splicing in HeLaMutation assay, REMSA, UV cross-linking in HeLa nuclear extract, Immunoblotting
hnRNP H2GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC-10Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11421362Hastings ML, Wilson CM, Munroe SH. (2001)
A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.
RNA. 7(6):859-874.
 Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing with HeLa NEUV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins.
hnRNP H2AGGGUUGAGGGGAGCAGGGU-7Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H2CGGGUUGACGGGAGCACGGU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H2AGUGUUGAGUGGAGCAGUGGU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H2GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP H2AAGAA-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2CAGGAC-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGAGA-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGAGG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGAG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGC-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGU-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGG-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2GGGGG-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2CGGGGGGGGC-6Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGGAGGGG-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGAAGGGA-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2GUAUCCUUCCCUGGCGGGGGUGGGAGAGCAGCAGGGCCAGGGGGGUGACACACC-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGGGAUGGGCGAGAUGGGGUGCUGU-10Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGGGAUGGGCGAGAUGGGGUGCUGU-4Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGUGAUGUGCGAGAUGGUGUGCUGU-8Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGUGAUGUGCGAGAUGGUGUGCUGU-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2GGUUGGGAGAGGGAUUUC-10Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2GGUUGUGAGAGUGAUUUC-1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H2GGGAAUGUGGG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 19506027Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009)
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.
Nucleic Acids Res. 37(14):4672-4683.
 Sequences deriving from HBB [3043] UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein
hnRNP H2GGGGU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H2UGGGA-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H2CUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2CUGAGGCUGGGGGCUGGAAUUCCCAUGUGCUUCCU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2UAUGACCCGGACUCCCGGUG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2UAUGAUAGGCACUUAGGCUG-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2CUGGGGGCUG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2GGGGC-2Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2AAGGCGAA1Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 15828859Han K, Yeo G, An P, Burge CB, Grabowski PJ. (2005)
A combinatorial code for splicing silencing: UAGG and GGGG motifs.
PLoS Biol. 3(5):e158.
In this context, hnRNP A1 was shown to mediate silencing while hnRNP H1, H2, F enhance exon inclusion. Synthesized sequences based on GRIN1 [2902] EX19. Mutational analysis, UV crosslinking, immunoprecipitation in HeLa nuclear extracts.
hnRNP H2UGGGU-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 18806275Masuda A, Shen XM, Ito M, Matsuura T, Engel AG, Ohno K. (2008)
hnRNP H enhances skipping of a nonfunctional exon P3A in CHRNA1 and a mutation disrupting its binding causes congenital myasthenic syndrome.
Hum Mol Genet. 17(24):4022-4035.
 Construct of CHRNA1 [1134] EX2-INT2-EX3-INT-EX_P3A-INT-EX4In vivo splicing assay in HeLaWestern blotting using HEK293T nuclear extract, immunodepletion, surface plasmon resonance
hnRNP H2CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H2CAGAAGGGGAGGGGUUCCA-3Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP H2AACAAGGGGUGGGGGAAAA-3Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP H2GGGGA-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H2GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 24290757Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V, Miller JW, Smith BN, Gallo JM, Ule J, Hirth F, Rogelj B, Houart C, Shaw CE. (2013)
Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic.
Cell Rep. 5(5):1178-1186.
 Synthetic sequences. RNA pull-down assay using SH-SY5Y cell extract.
hnRNP H2UGGGACUGGGACUGGGACUG-5Gene Name and Synonymous: HNRNPH2, heterogeneous nuclear ribonucleoprotein H2 (H'), FTP3, HNRPH', HNRPH2, hnRNPH'. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP H3AGGGC-6Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGU-6Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGA-6Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGG-6Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3GGGGG-6Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3CGGGGGGGGC-6Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGGAGGGG-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGAAGGGA-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3AGGGAAGGGAAGGGAAGGGAAGGGAAGGGAAGGGA-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3UGGGC-2Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3CGGGC-2Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP H3GGGGGGG-7Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP H3UGUGGG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 10072387Chen CD, Kobayashi R, Helfman DM. (1999)
Binding of hnRNP H to an exonic splicing silencer is involved in the regulation of alternative splicing of the rat beta-tropomyosin gene.
Genes Dev. 13(5):593-606.
 Construct of rat beta-tropomyosin Tpm2 [500450] EX5-INT5-EX7In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assays, immunoblot, immunodepletion in HeLa nuclear extracts.
hnRNP H3GGGGGCUG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
hnRNP H3GGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC-10Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 11421362Hastings ML, Wilson CM, Munroe SH. (2001)
A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.
RNA. 7(6):859-874.
 Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing with HeLa NEUV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins.
hnRNP H3AUUGGGUGU-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 11526107Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001)
A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H.
J Biol Chem. 276(44): 40464-75.
 Constructs of HIV-1 Tat [155871] EX2.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.EMSA, UV crosslink, immunoprecipitation and immunoblot.
hnRNP H3AUCGGGCGU-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 11526107Jacquenet S, Mereau A, Bilodeau PS, Damier L, Stoltzfus CM, Branlant C. (2001)
A second exon splicing silencer within human immunodeficiency virus type 1 tat exon 2 represses splicing of Tat mRNA and binds protein hnRNP H.
J Biol Chem. 276(44): 40464-75.
 Constructs of HIV-1 Tat [155871] EX2.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.EMSA, UV crosslink, immunoprecipitation and immunoblot.
hnRNP H3CGGGA5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
hnRNP H3GGAGGA-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP H3AAGGUG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
hnRNP H3UGGGG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H3GGGGU-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H3UGGGA-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 12732620Pagani F, Buratti E, Stuani C, Baralle FE. (2003)
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
J Biol Chem. 278(29):26580-26588.
 Construct of CFTR [1080] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in Hep3B cells of WT and mutant CFTR constructs.UV cross-linking, pull-down assay, immunoblotting in HeLa nuclear extracts with wt and mutated RNAs.
hnRNP H3AGGGUUGAGGGGAGCAGGGU-7Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H3CGGGUUGACGGGAGCACGGU-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H3AGUGUUGAGUGGAGCAGUGGU-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
hnRNP H3GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP H3GUAUCCUUCCCUGGCGGGGGUGGGAGAGCAGCAGGGCCAGGGGGGUGACACACC-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGGGAUGGGCGAGAUGGGGUGCUGU-10Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGGGAUGGGCGAGAUGGGGUGCUGU-4Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3AUAUCCUGCCCAAGAUGGGAGAGGCGGGAGGGGUUAGUGAUGUGCGAGAUGGUGUGCUGU-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3AUAUCCUGCCCAAGAUGUGAGAGGCGUGAGGUGUUAGUGAUGUGCGAGAUGGUGUGCUGU-1Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3GGUUGGGAGAGGGAUUUC-10Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3GGUUGUGAGAGUGAUUUC-1Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16308319McNally LM, Yee L, McNally MT. (2006)
Heterogeneous nuclear ribonucleoprotein H is required for optimal U11 small nuclear ribonucleoprotein binding to a retroviral RNA-processing control element: implications for U12-dependent RNA splicing.
J Biol Chem. 281(5):2478-2488.
 Sequences deriving from wt and mutated RSV NRS region, SCN4A [6329], NOP2 [4839]. Constructs of EX_[RSV NRS] - INT_[RSV NRS] - NOP2 [4839] INT - NOP2 [4839] EX. Constructs of SCN4A [6329] EX2 - INT2 - EX3. Constructs of NOP2 [4839] EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking, MALDI-TOF and western blot with HeLa NE
hnRNP H3GAUCACUGGGGUGGAUCAUCCAGGUGGGGCUUUU-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16396608Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006)
Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.
PLoS Biol. 4(2):e21.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEImmunoprecipitation, Gel shift and RNase H protection assays
hnRNP H3GGGUCGAGGGG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16396608Martinez-Contreras R, Fisette JF, Nasim FU, Madden R, Cordeau M, Chabot B. (2006)
Intronic binding sites for hnRNP A/B and hnRNP F/H proteins stimulate pre-mRNA splicing.
PLoS Biol. 4(2):e21.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEImmunoprecipitation, Gel shift and RNase H protection assays
hnRNP H3CAGAAGGGGAGGGGUUCCA-3Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP H3AACAAGGGGUGGGGGAAAA-3Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP H3UGGGU-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 18806275Masuda A, Shen XM, Ito M, Matsuura T, Engel AG, Ohno K. (2008)
hnRNP H enhances skipping of a nonfunctional exon P3A in CHRNA1 and a mutation disrupting its binding causes congenital myasthenic syndrome.
Hum Mol Genet. 17(24):4022-4035.
 Construct of CHRNA1 [1134] EX2-INT2-EX3-INT-EX_P3A-INT-EX4In vivo splicing assay in HeLaWestern blotting using HEK293T nuclear extract, immunodepletion, surface plasmon resonance
hnRNP H3GGGAAUGUGGG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 19506027Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009)
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.
Nucleic Acids Res. 37(14):4672-4683.
 Sequences deriving from HBB [3043] UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein
hnRNP H3GAUCACUGGGGUGGAUCAU-1Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H3GAUCACUGUGGUGGAUCAUCCAGGUGUGGCUUUU-1Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H3GAUCACUGUGGUGGAUCAUCCAGGUGGGGCUUUU-1Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H3CCAUGGUUUGGGAGUGGGAAGGUGGGGAG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H3CCAUGGUUUGGGGGCAGUAGUUGG-8Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 19926721Fisette JF, Toutant J, Dugre-Brisson S, Desgroseillers L, Chabot B. (2010)
hnRNP A1 and hnRNP H can collaborate to modulate 5' splice site selection.
RNA. 16(1):228-238.
 Constructs of hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - EXL2_[Adenovirus] and hnRNP A1 [15382] EX7/EX7B - INT_[lambda phage DNA] - BCL2L1 [598] EX3In vitro splicing with HeLa NEFilter binding assay, EMSA using recombinant protein
hnRNP H3GGGGA-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP H3GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 24290757Lee YB, Chen HJ, Peres JN, Gomez-Deza J, Attig J, Stalekar M, Troakes C, Nishimura AL, Scotter EL, Vance C, Adachi Y, Sardone V, Miller JW, Smith BN, Gallo JM, Ule J, Hirth F, Rogelj B, Houart C, Shaw CE. (2013)
Hexanucleotide repeats in ALS/FTD form length-dependent RNA foci, sequester RNA binding proteins, and are neurotoxic.
Cell Rep. 5(5):1178-1186.
 Synthetic sequences. RNA pull-down assay using SH-SY5Y cell extract.
hnRNP H3AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-4Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP H3UGGGACUGGGACUGGGACUG-5Gene Name and Synonymous: HNRNPH3, heterogeneous nuclear ribonucleoprotein H3 (2H9), hnRNP 2H9, HNRPH3, FLJ34092. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
hnRNP I (PTB)CUCUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
hnRNP I (PTB)UCUUC-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11788707Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002)
Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein.
Nucleic Acids Res. 30(2):456-462.
 Synthesized sequences Chemical shift mapping (NMR)
hnRNP I (PTB)CCUCUGCGCUUCUUCCCUUCCCUCCUCCCUGGCUCAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11931771Charlet-B N, Logan P, Singh G, Cooper TA. (2002)
Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.
Mol Cell. 9(3):649-658.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.UV crosslink, western blot, immunoprecipitation in HeLa nuclear extracts.
hnRNP I (PTB)UUAUUUUUCCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9858533Lou H, Helfman DM, Gagel RF, Berget SM. (1999)
Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon.
Mol Cell Biol. 19(1):78-85.
 Constructs of CALCA [796] EX4 - INT4 - EX5 - INT5 - EX6 fused to a heterologous first exon from adenovirus used for in vivo splicing.In vivo splicing in HeLa and T98G cells.EMSA, UV crosslink, immunoprecipitation with recombinant protein.
hnRNP I (PTB)UUUUUUU-7Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
8449401Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B. (1993)
Cloning and characterization of PSF, a novel pre-mRNA splicing factor.
Genes Dev. 7(3):393-406.
PSF has no affinity to poly(rA), poly(rC) and poly(rG). Construct of tropomyosin 1 alpha TPM1 [7168] EX2 - INT2 - EX3 for splicing. Construct of branchpoint and polypyrimidine tract element upstream of TPM1 EX3 for UV-crosslink.In vitro splicing in HeLa nuclear extracts.RNA affinity chromatography confirmed by UV crosslink and Western blot using HeLa nuclear extracts.
hnRNP I (PTB)CUCCGCUCCUCUUC5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9858533Lou H, Helfman DM, Gagel RF, Berget SM. (1999)
Polypyrimidine tract-binding protein positively regulates inclusion of an alternative 3'-terminal exon.
Mol Cell Biol. 19(1):78-85.
 Constructs of CALCA [796] EX4 - INT4 - EX5 - INT5 - EX6 fused to a heterologous first exon from adenovirus used for in vivo splicing.In vivo splicing in HeLa and T98G cells.EMSA, UV crosslink, immunoprecipitation with recombinant protein.
hnRNP I (PTB)UCUUCUU-8Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9436911Gooding C, Roberts GC, Smith CW. (1998)
Role of an inhibitory pyrimidine element and polypyrimidine tract binding protein in repression of a regulated alpha-tropomyosin exon.
RNA. 4(1): 85-100.
 Constructs of rat alpha-tropomyosin (TM) Tpm2 [24851] EX1 - INT - EX3 - INT3 - EX4 and EX2 - INT2 - EX3 - INT3 - EX4.In vivo splicing in transiently transfected L fibroblast and PA smooth muscle cells in vitro splicing in HeLa nuclear extract.
hnRNP I (PTB)CCUUCCUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16260624Lin JC, Tarn WY. (2005)
Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB.
Mol Cell Biol. 25(22): 10111-10121.
PTB and RBM4 are in competition for CU1 element. PTB appear to bind with more affinity than RBM4. Construct of alpha-TM [TPM1, 7168] EX8 - INT8 - EX9a - INT9a - EX9b and EX1 - INT1 - EX2b - INT2b - EX3.In vivo splicing in HEK293 cells.Mutational analysis.
hnRNP I (PTB)UUCUUCUUCUUCUUCUUCUUCUUCUUCUUC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
hnRNP I (PTB)UCUUU-6Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11788707Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002)
Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein.
Nucleic Acids Res. 30(2):456-462.
 Synthesized sequences Chemical shift mapping (NMR)
hnRNP I (PTB)UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP I (PTB)UCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU-2Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP I (PTB)CCUUUCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
hnRNP I (PTB)UCUUUCUCCUUUUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19147685Bian Y, Masuda A, Matsuura T, Ito M, Okushin K, Engel AG, Ohno K. (2009)
Tannic acid facilitates expression of the polypyrimidine tract binding protein and alleviates deleterious inclusion of CHRNA1 exon P3A due to an hnRNP H-disrupting mutation in congenital myasthenic syndrome.
Hum Mol Genet. 18(7):1229-1237.
 Construct of CHRNA1 [1134] INT3 - EX_P3A - INT_P3A.In vivo splicing in HEK293T and HeLa cells. Downregulation by siRNA and upregulation by overexpression.In vitro UV cross-linking and immunoprecipitation, Immunoblotting, Surface plasmon resonance analysis.
hnRNP I (PTB)UCUUCUCUCUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
12649496Gromak N, Matlin AJ, Cooper TA, Smith CW. (2003)
Antagonistic regulation of alpha-actinin alternative splicing by CELF proteins and polypyrimidine tract binding protein.
RNA. 9(4):443-456.
 Construct of rat Actn1 [81634] EX_NM - INTIn vitro splicing with HeLa nuclear extracts.UV cross-link with recombinant protein and with HeLa nuclear extract containing increasing protein concentrations. Mutation analysis.
hnRNP I (PTB)CUUCUCUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
15840818Amir-Ahmady B, Boutz PL, Markovtsov V, Phillips ML, Black DL. (2005)
Exon repression by polypyrimidine tract binding protein.
RNA. 11(5):699-716.
 Several constructs based on beta globin HBB [3043] and SRC [20779]In vitro splicing with HeLa and WERI nuclear extracts.UV-crosslink, EMSA
hnRNP I (PTB)CUUUCUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
hnRNP I (PTB)UUUCUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
hnRNP I (PTB)UCUUUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
hnRNP I (PTB)UUACUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
hnRNP I (PTB)UCUAUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
hnRNP I (PTB)UCCCUUUUUUUUCCACAG-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP I (PTB)CCUUCUUCUUUUUCCUACAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP I (PTB)CCCUUUUUUUUCCACAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP I (PTB)CCCUUUUUUUUCCGGAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP I (PTB)GCAGCCUGGUGCCUCCCUCUUGGCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9261396Tsuchihara K, Tanaka T, Hijikata M, Kuge S, Toyoda H, Nomoto A, Yamamoto N, Shimotohno K. (1997)
Specific interaction of polypyrimidine tract-binding protein with the extreme 3'-terminal structure of the hepatitis C virus genome, the 3'X.
J Virol. 71(9):6720-6726
 Synthesized sequences UV cross-linking, immunoprecipitation, competition assay in PH5CH cells
hnRNP I (PTB)CCUUUUCCUUCUUCUUAUU-8Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9292499Ashiya M, Grabowski PJ. (1997)
A neuron-specific splicing switch mediated by an array of pre-mRNA repressor sites: evidence of a regulatory role for the polypyrimidine tract binding protein and a brain-specific PTB counterpart.
RNA. 3(9):996-1015.
 Constructs of rat GABRG2 [29709] EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing in HeLa nuclear extract.UV cross-linking, competition assay, immunoprecipitation
hnRNP I (PTB)UGUUUCUCUUUCUCUCCUUU-2Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9292499Ashiya M, Grabowski PJ. (1997)
A neuron-specific splicing switch mediated by an array of pre-mRNA repressor sites: evidence of a regulatory role for the polypyrimidine tract binding protein and a brain-specific PTB counterpart.
RNA. 3(9):996-1015.
 Constructs of rat GABRG2 [29709] EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing in HeLa nuclear extract.UV cross-linking, competition assay, immunoprecipitation
hnRNP I (PTB)GCAAUUCUCUUUUCUGUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9292499Ashiya M, Grabowski PJ. (1997)
A neuron-specific splicing switch mediated by an array of pre-mRNA repressor sites: evidence of a regulatory role for the polypyrimidine tract binding protein and a brain-specific PTB counterpart.
RNA. 3(9):996-1015.
 Constructs of rat GABRG2 [29709] EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing in HeLa nuclear extract.UV cross-linking, competition assay, immunoprecipitation
hnRNP I (PTB)UCUUUCCUUCUUUUUUCCUUUCUUUUCCUUCCUUCUUUAAU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
10037806Gontarek RR, Gutshall LL, Herold KM, Tsai J, Sathe GM, Mao J, Prescott C, Del Vecchio AM. (1999)
hnRNP C and polypyrimidine tract-binding protein specifically interact with the pyrimidine-rich region within the 3'NTR of the HCV RNA genome.
Nucleic Acids Res. 27(6):1457-1463.
 HCV 3' NTR UV cross-linking, immunoprecipitation, competition assay with HepG2 cell extract
hnRNP I (PTB)UUUCUCCUCUUCUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
10856256Conte MR, Grune T, Ghuman J, Kelly G, Ladas A, Matthews S, Curry S. (2000)
Structure of tandem RNA recognition motifs from polypyrimidine tract binding protein reveals novel features of the RRM fold.
EMBO J. 19(12):3132-3141.
 Synthesized sequences Filter binding assay using recombinant protein
hnRNP I (PTB)UCUCU-6Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)UCCUCUUC-3Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11788707Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002)
Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein.
Nucleic Acids Res. 30(2):456-462.
 Synthesized sequences Chemical shift mapping (NMR)
hnRNP I (PTB)UCUUCUCU-6Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11788707Yuan X, Davydova N, Conte MR, Curry S, Matthews S. (2002)
Chemical shift mapping of RNA interactions with the polypyrimidine tract binding protein.
Nucleic Acids Res. 30(2):456-462.
 Synthesized sequences Chemical shift mapping (NMR)
hnRNP I (PTB)CCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP I (PTB)CAGCCACCUCUCCCCUCUCCGCACUGCUGCCA-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP I (PTB)UACCACCACCCCUUCCCCAUUCCCUUGCCCUGCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP I (PTB)CUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP I (PTB)GUCUCUCUCUCUCAACCUCUUUCUUCCAAUCUCUCUUUCUCAAUCUCUCUGUUUCCCUUUGUC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
12517964Kosinski PA, Laughlin J, Singh K, Covey LR. (2003)
A complex containing polypyrimidine tract-binding protein is involved in regulating the stability of CD40 ligand (CD154) mRNA.
J Immunol. 170(2):979-988.
 Sequences deriving from CD40LG [959] UV cross-linking, EMSA with Jurkat total extracts or immunodepleted extracts
hnRNP I (PTB)UUUGUCUUCUUCUUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16109372Izquierdo JM, Majos N, Bonnal S, Martinez C, Castelo R, Guigo R, Bilbao D, Valcarcel J. (2005)
Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition.
Mol Cell. 19(4):475-484.
 Sequences deriving from wt and mutated FAS [355]. Construct of FAS [355] EX5 - INT5 - EX6 - INT6 - EX7In vitro splicing in HeLa NEUV cross-linking, immunoprecipitation with HeLa NE
hnRNP I (PTB)CUCUCUAAAAACUCUCU-2Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)CUCUCUAAAAAAAAAACUCUCU-4Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)CUCUCUAAAAAAAAAAAAAAACUCUCU-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)CUCUCUAAAAAAAAAAAAAAAAAAAACUCUCU-8Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)CUCUCUAAAAAAAAAAAAAAAAAAAAAAAAACUCUCU-6Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)CUCUCUAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACUCUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16179478Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FH. (2005)
Structure of PTB bound to RNA: specific binding and implications for splicing regulation.
Science. 309(5743):2054-2057.
 Synthesized sequences NMR, EMSA with recombinant protein
hnRNP I (PTB)UAACACCCAGUCUGUUCCCCAUGG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16950790Pautz A, Linker K, Hubrich T, Korhonen R, Altenhofer S, Kleinert H. (2006)
The polypyrimidine tract-binding protein (PTB) is involved in the post-transcriptional regulation of human inducible nitric oxide synthase expression.
J Biol Chem. 281(43):32294-32302.
 Sequences deriving from NOS2 [4843] UV cross-linking using recombinant protein
hnRNP I (PTB)GAGUUUUCUCCUCUCUCAACUUGUUCUCUCUCCUUCUUU-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16982681Sauliere J, Sureau A, Expert-Bezancon A, Marie J. (2006)
The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.
Mol Cell Biol. 26(23):8755-8769.
 Sequences deriving from chicken TPM3 [396430]. Constructs of chicken TPM3 [396430] EX5 - INT5 - EX6A - INT6 - EX6B - INT6 - EX7.In vitro splcing in HeLa NEEMSA with recombinant protein
hnRNP I (PTB)CCUCCUUCCCUGUGCUCCUUCCGCUCCCACCUUCUUCCCUU-1Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16982681Sauliere J, Sureau A, Expert-Bezancon A, Marie J. (2006)
The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.
Mol Cell Biol. 26(23):8755-8769.
 Sequences deriving from chicken TPM3 [396430]. Constructs of chicken TPM3 [396430] EX5 - INT5 - EX6A - INT6 - EX6B - INT6 - EX7.In vitro splcing in HeLa NEEMSA with recombinant protein
hnRNP I (PTB)UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17466948Reyes R, Izquierdo JM. (2007)
The RNA-binding protein PTB exerts translational control on 3'-untranslated region of the mRNA for the ATP synthase beta-subunit.
Biochem Biophys Res Commun. 357(4):1107-1112.
 Sequences deriving from ATP5B [506] EMSA supershift, UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein.
hnRNP I (PTB)UUGUUUGAUUUCUUAAAGU-8Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17507659Hall-Pogar T, Liang S, Hague LK, Lutz CS. (2007)
Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR.
RNA. 13(7):1103-1115.
 Sequences deriving from PTGS2 [5743] Western blot with HeLa NE
hnRNP I (PTB)GGCGAGAAAUAACUCAUUUCUCCUUUUUUUCUUCUCUCUUCUGUCUGAAUUCUCCCUGUCUCCCUUCCUGUGGGCCAUGGGGCUCA-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17592047Matlin AJ, Southby J, Gooding C, Smith CW. (2007)
Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract.
RNA. 13(8):1214-1223.
 Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM.In vitro splicing in HeLa NEUV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein.
hnRNP I (PTB)GGCUAUCUUUCCUGGGAACCUGCUUGGGUAUCCGCCUCCUCUCCCUCGUCACAUCUCACCUGUGGACUGGUCUUCUGCAUUUCUUUGCUCUG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17592047Matlin AJ, Southby J, Gooding C, Smith CW. (2007)
Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract.
RNA. 13(8):1214-1223.
 Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM.In vitro splicing in HeLa NEUV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein.
hnRNP I (PTB)GGGCCCUUCCUCUUUCUGCUGUCCUCCUGGCCUCUGCCUGGGCCCUCCUCCUCCCACCUGUCUGUCCCUCCUGUGUCUUGGCACCACUGCCCACAG-7Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17592047Matlin AJ, Southby J, Gooding C, Smith CW. (2007)
Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract.
RNA. 13(8):1214-1223.
 Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM.In vitro splicing in HeLa NEUV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein.
hnRNP I (PTB)GGCGAGAAAUAACUCAUUUCUCCUUUUUUUCUUCUCUCUUCUAUCUAAAUUCUCCCUGUCUCCCUUCCUGUGGGCCAUGGGGCUCA-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17592047Matlin AJ, Southby J, Gooding C, Smith CW. (2007)
Repression of alpha-actinin SM exon splicing by assisted binding of PTB to the polypyrimidine tract.
RNA. 13(8):1214-1223.
 Sequences deriving from rat Actn1 [81634]. Constructs of rat Actn1 [81634] EX_NM - INT - EX_SM.In vitro splicing in HeLa NEUV cross-linking and immunoprecipitation with HeLa NE. UV cross-linking and EMSA with recombinant protein.
hnRNP I (PTB)UCUCCUUGUUUUGCUUUCGAUCUGGACUGUUCUCAGGCAAGCCGGGGAGUAACUUUUAGUUUUGCUCCUGCGAUUAUUCAACUGACGGGCUUUCAUUUCCAUUUCACAUACCCUAGCAACACUUAUACCUUGCGGAAUUGUAUUGGUAGCGUGAAAAAAGCACACUGAGAGG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
17698501Dhar D, Roy S, Das S. (2007)
Translational control of the interferon regulatory factor 2 mRNA by IRES element.
Nucleic Acids Res. 35(16):5409-5421.
 Sequences deriving from IRF2 [3660] UV cross-linking with recombinant protein
hnRNP I (PTB)CCUCUCCUUCUCUCUGCUUCUCUCUCGCUGGCCCUUAGGAGGAAGGUGGAUGUCAGGUGUGUACCGAGG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19226116Clerte C, Hall KB. (2009)
The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
Biochemistry. 48(10):2063-2074.
 Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] EMSA with recombinant protein
hnRNP I (PTB)UCCUUCUCUCUGCUUCUCUCUCGCUGGCCCUUAGGAGG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19226116Clerte C, Hall KB. (2009)
The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
Biochemistry. 48(10):2063-2074.
 Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] EMSA with recombinant protein
hnRNP I (PTB)GGAUGCUUCGCUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCUCCACCGCCCCUGUGUGUUUCCAGCUCUCUCCCCGUCCCUUUAGCUUACCCUGCAUCCCACCUGUAUGAGCCGACCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19226116Clerte C, Hall KB. (2009)
The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
Biochemistry. 48(10):2063-2074.
 Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] EMSA with recombinant protein
hnRNP I (PTB)GCAAUUCUCUUUUCUGUCUACAAAUCCAAAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19226116Clerte C, Hall KB. (2009)
The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
Biochemistry. 48(10):2063-2074.
 Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] EMSA with recombinant protein
hnRNP I (PTB)GAGACUUGGUGGCUCCAUCUUAGCCCUA-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19226116Clerte C, Hall KB. (2009)
The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.
Biochemistry. 48(10):2063-2074.
 Sequences deriving from HCV 3' NTR, mouse Gabrg2 [14406] and Src [20779] EMSA with recombinant protein
hnRNP I (PTB)CACUAAGCUCGCUUUCUUGCUGUCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19506027Millevoi S, Decorsiere A, Loulergue C, Iacovoni J, Bernat S, Antoniou M, Vagner S. (2009)
A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.
Nucleic Acids Res. 37(14):4672-4683.
 Sequences deriving from HBB [3043] UV cross-linking, immunoprecipitation with HeLa NE and recombinant protein
hnRNP I (PTB)UCCUCCUCCUUCCCUCUUCCUUGCCCCCUCUUCCCCUAAACCUUACAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
20010808David CJ, Chen M, Assanah M, Canoll P, Manley JL. (2010)
HnRNP proteins controlled by c-Myc deregulate pyruvate kinase mRNA splicing in cancer.
Nature. 463(7279):364-368.
 Sequences deriving from PKM2 [5315]. Construct of PKM2 [5315] EX9 - INT9 - AdML. Construct of PKM2 [5315] EX8 - INT8 - EX9 - INT9 - EX10 - INT10 - EX11.In vitro splicing with HeLa NE. In vivo splicing in HeLa.Protein affinity purification, mass spectrometry, UV cross-linking and immunoblotting with HeLa NE. siRNA.
hnRNP I (PTB)UUUCUCUUUCUCUCCUUUCCUUUUCCUUCUUCUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16431980Clerte C, Hall KB. (2006)
Characterization of multimeric complexes formed by the human PTB1 protein on RNA.
RNA. 12(3):457-475.
 Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR EMSA with recombinant protein. RNA footprinting.
hnRNP I (PTB)UUCUCUUUUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16431980Clerte C, Hall KB. (2006)
Characterization of multimeric complexes formed by the human PTB1 protein on RNA.
RNA. 12(3):457-475.
 Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR EMSA with recombinant protein. RNA footprinting.
hnRNP I (PTB)UAGCUGUG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16431980Clerte C, Hall KB. (2006)
Characterization of multimeric complexes formed by the human PTB1 protein on RNA.
RNA. 12(3):457-475.
 Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR EMSA with recombinant protein. RNA footprinting.
hnRNP I (PTB)CUCCAUCUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
16431980Clerte C, Hall KB. (2006)
Characterization of multimeric complexes formed by the human PTB1 protein on RNA.
RNA. 12(3):457-475.
 Sequences deriving from rat Gabrg2 [29709] and HCV 3' NTR EMSA with recombinant protein. RNA footprinting.
hnRNP I (PTB)GGCUAACUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCUCUCUCUCUCUUUCCCGC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9305981Perez I, McAfee JG, Patton JG. (1997)
Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein.
Biochemistry. 36(39):11881-11890.
 Sequences deriving from rat Tpm1 [24851] and synthesized oligos. UV cross-linking with HeLa NE and recombinant protein. Fluorescence spectroscopy with recombinant protein.
hnRNP I (PTB)CCCCCCC-7Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9305981Perez I, McAfee JG, Patton JG. (1997)
Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein.
Biochemistry. 36(39):11881-11890.
 Sequences deriving from rat Tpm1 [24851] and synthesized oligos. UV cross-linking with HeLa NE and recombinant protein. Fluorescence spectroscopy with recombinant protein.
hnRNP I (PTB)GGGGGGG-2Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9305981Perez I, McAfee JG, Patton JG. (1997)
Multiple RRMs contribute to RNA binding specificity and affinity for polypyrimidine tract binding protein.
Biochemistry. 36(39):11881-11890.
 Sequences deriving from rat Tpm1 [24851] and synthesized oligos. UV cross-linking with HeLa NE and recombinant protein. Fluorescence spectroscopy with recombinant protein.
hnRNP I (PTB)CUGAGGCUGGGGGCUGCUCUCUGCAUGUGCUUCCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP I (PTB)CUUUCCUUUCAUUCUUUCACUUCUCU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11931771Charlet-B N, Logan P, Singh G, Cooper TA. (2002)
Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.
Mol Cell. 9(3):649-658.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.UV crosslink, western blot, immunoprecipitation in HeLa nuclear extracts.
hnRNP I (PTB)UCUCUC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11931771Charlet-B N, Logan P, Singh G, Cooper TA. (2002)
Dynamic antagonism between ETR-3 and PTB regulates cell type-specific alternative splicing.
Mol Cell. 9(3):649-658.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts. In vivo splicing in HeLa.UV crosslink, western blot, immunoprecipitation in HeLa nuclear extracts.
hnRNP I (PTB)UUCUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19016857Raponi M, Buratti E, Llorian M, Stuani C, Smith CW, Baralle D. (2008)
Polypyrimidine tract binding protein regulates lternative splicing of an aberrant pseudoexon in NF1.
FEBS J. 275(24): 6101-6108.
 Construct of NF1 [4763] partialEX30 - INT30 - partialEX31.In vivo splicing in Hela.Mutagenesis, Pull down assay and Western blot with HeLa nucelar extract.
hnRNP I (PTB)UUCUUC-8Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19016857Raponi M, Buratti E, Llorian M, Stuani C, Smith CW, Baralle D. (2008)
Polypyrimidine tract binding protein regulates lternative splicing of an aberrant pseudoexon in NF1.
FEBS J. 275(24): 6101-6108.
 Construct of NF1 [4763] partialEX30 - INT30 - partialEX31.In vivo splicing in Hela.Mutagenesis, Pull down assay and Western blot with HeLa nucelar extract.
hnRNP I (PTB)GUCUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
19016857Raponi M, Buratti E, Llorian M, Stuani C, Smith CW, Baralle D. (2008)
Polypyrimidine tract binding protein regulates lternative splicing of an aberrant pseudoexon in NF1.
FEBS J. 275(24): 6101-6108.
 Construct of NF1 [4763] partialEX30 - INT30 - partialEX31.In vivo splicing in Hela.Mutagenesis, Pull down assay and Western blot with HeLa nucelar extract.
hnRNP I (PTB)CUCUUC-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)CUCUUA-7Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)AUCUUC-7Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)CUCUUU-7Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)CUCUUG-4Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)UUCUUG-4Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)GUCUUA-4Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
9214659Perez I, Lin CH, McAfee JG, Patton JG. (1997)
Mutation of PTB binding sites causes misregulation of alternative 3' splice site selection in vivo.
RNA. 3(7): 764-778.
 Constructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 of WT and mutants for in vivo splicing. Constructs of TPM1 branch point and polypirimidine tract upstream EX3 and synthetised oligo for UV-crosslink. Sequences of 20 nt random for SELEX.In vivo splicing assay by transfecting constructs into smooth muscle cells (SMC) and HeLa cells.SELEX of 20nt random with recombinant protein. UV crosslink and SDS-PAGE with HeLa nuclear extract. Equilibrium binding assays with another protein or other RNAs as competitors.
hnRNP I (PTB)UUCACAUUUCAUUCCCUUGUGUUUCUGUCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)CUUGACGCCUGGUGCCUCUCUCUGGCCCCCG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)UAGCAUCAGCCUGGUGCCUACCUUCGGCCCC-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)CUUGCUUCACCUGGUGCCUUCCCUUCGGCCC-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)GCUCCAGCCUGGUGGCACGCUCCUGUCCCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)AGCUGCAGCCUGGAGCUCCUCUCGUGGCCCC-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)GGCUCAGCCUGCUGACCCUUCUUCCGUCGCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)AGUGGCUGUCUGAUGCUGCUCUUCCAGCUCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)GGUAGAUGCCUGCAGCCGUUCUUCCGGUGCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)AGCCGCUGUCUGCAGACUUCCCUUCGUCGCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)GCUGUCCUGUCUUCUCCUUCUUUCCUGGUCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)ACUGUCGUCUAUUACUCUGUUCUGUUCUUCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)UUCCGUCUCACUUCUUCUUUCCGCUGUCCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)UUCCUCUCUUCCUUAUACCGUUCUGUGUGCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)UCUAGCUUUCCUUUCCUCUUCUCUCUUCCCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)UUUUUGUUGUUUUUUUU-1Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
7761834Singh R, Valcarcel J, Green MR. (1995)
Distinct binding specificities and functions of higher eukaryotic polypyrimidine tract-binding proteins.
Science. 268(5214):1173-1176.
 Sequences of 31nt random for SELEX. Sequence deriving from Ad2 Major Late transcript and D. melanogaster tra [39849]. SELEX of 31nt random and EMSA with recombinant protein.
hnRNP I (PTB)CCUCUUU-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
21518792Lin JC, Tarn WY. (2011)
RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing.
J Cell Biol. 193(3):509-520.
 Constructs of wt or mutated TPM1 [7168]_EX8 - Ptbp1 [19205]_INT10 - Ptbp1 [19205]_EX11 - Ptbp1 [19205]_INT11 - TPM1 [7168]_ EX9BIn vivo splicing in C2C12 cells overexpressing human recombinant proteinsMutagenesis, in vivo splicing assay
hnRNP I (PTB)UUUAGUCAGCCUUAUAGCUAA-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
hnRNP I (PTB)CACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP I (PTB)CCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU-1Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP I (PTB)CCACGCACGCAGACUCGCAG-2Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP I (PTB)CACGCAGACAUUCAGCGGCU-2Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP I (PTB)GCCCAAGGAGGUGAUAGCAUUUUUCAGAGAUUGAAAAGAAUAGUAUUUGAUGCCAAAUCUACAAUUGUG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
24499931Han A, Stoilov P, Linares AJ, Zhou Y, Fu XD, Black DL. (2014)
De novo prediction of PTBP1 binding and splicing targets reveals unexpected features of its RNA recognition and function.
PLoS Comput Biol. 10(1):e1003442.
 Sequences deriving from mouse Tmx3 [67988] and Scrib [105782]. EMSA with recombinant protein
hnRNP I (PTB)GUGAGGGCUCUUUCUUCGUGGGACCGUAGAUAGGUAGCUGCUGCUGGUCUCACACUGUUCUCCCUACAG-10Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
24499931Han A, Stoilov P, Linares AJ, Zhou Y, Fu XD, Black DL. (2014)
De novo prediction of PTBP1 binding and splicing targets reveals unexpected features of its RNA recognition and function.
PLoS Comput Biol. 10(1):e1003442.
 Sequences deriving from mouse Tmx3 [67988] and Scrib [105782]. EMSA with recombinant protein
hnRNP I (PTB)GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-6Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP I (PTB)AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-4Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP I (PTB)UCCCUUUUUUUUCCACCC-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP I (PTB)UCCCUCCCUCCCUCCACAG-5Gene Name and Synonymous: PTBP1, polypyrimidine tract binding protein 1, PTB, PTB2, PTB3, PTB4, pPTB, PTB-1, PTB-T, MGC8461, MGC10830, HNRPI, HNRNPI, HNRNP-I.
In the context of CALCA gene, PTB enhances exon 4 inclusion (PMID:9858533).
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
2533575Garcia-Blanco MA, Jamison SF, Sharp PA. (1989)
Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.
Genes Dev. 3(12A):1874-1886.
 Synthesized sequences UV cross-linking, immunoprecipitation in HeLa
hnRNP JCCCCCCC-7Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
Isoform: isoform J is due to Alternative Splicing.
3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP KCCCCCCC-7Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP KGGGGGGG-4Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
7607214Leffers H, Dejgaard K, Celis JE. (1995)
Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.
Eur J Biochem. 230(2): 447-453.
hnRNP E1 has no affinity to poly(rA). hnRNP E2 and hnRNP K have no affinity to poly(rA) and poly(rU). homopolymers Western blot of AMA (human amnion) cell extracts and homoribopolymers. Western blot of recombinant proteins and homoribopolymers.
hnRNP KCACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP KCCCCACCCUCUUCCCCAAGCCCCACCCUCUUCCCCAAG-8Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
9160751Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M, Hentze MW. (1997)
mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end.
Cell. 16
 Constructs of luciferase gene reporter.In vivo splicing assay with luciferase gene reporter in HeLaUV cross-linking. Cell-free translation of different mRNAs.
hnRNP KACCCAA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCAU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCCAA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCCCAA-2Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCCCAC-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCCCCCCCUA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCGA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCGC-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCGU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KACCCUU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KGCCCAA-2Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KGCCCAC-2Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KGCCCAG-2Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KGCCCCCCUA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KGCCCCUA-4Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCAA-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCAC-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCAU-2Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCCAA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCCAU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCCCAU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCCUA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCCUU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCGA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCUA-10Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KUCCCUU-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11278705Thisted T, Lyakhov DL, Liebhaber SA. (2001)
Optimized RNA targets of two closely related triple KH domain proteins, heterogeneous nuclear ribonucleoprotein K and alphaCP-2KL, suggest Distinct modes of RNA recognition.
J Biol Chem. 276(20):17484-17496.
 Sequences of 50nt random for SELEX. SELEX of random 50nt with recombinant protein.
hnRNP KCCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP KCAGCCACCUCUCCCCUCUCCGCACUGCUGCCA-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP KUACCACCACCCCUUCCCCAUUCCCUUGCCCUGCU-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP KCUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP KCCCUCUGCCACCCAGGCAGGCCCUGCCUUCAGCCCUGGCCCAGAGCUGGAACACUCUCUGAGAUGCCCCUCUGCCUGGGCUUAUGCCCUCAGAUGGAGACAUUGGAUGUGGAGCUCCUGCUGGAUGCGUGCCCUGACCCCUGCACCAGCCCUUCCCUGCUUUGAG-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
12600897Skalweit A, Doller A, Huth A, Kahne T, Persson PB, Thiele BJ. (2003)
Posttranscriptional control of renin synthesis: identification of proteins interacting with renin mRNA 3'-untranslated region.
Circ Res. 92(4):419-427.
 Sequences deriving from REN [5972] EMSA, UV cross-linking with Calu-6 cell extracts. RNA-affinity chromatography with MALDI-TOF-MS. Western blot.
hnRNP KUCCCCCCAA-10Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
15514164Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004)
RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis.
Circ Res. 95(11):1058-1066.
 Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF.
hnRNP KUCCCCCAA-10Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
15514164Thiele BJ, Doller A, Kahne T, Pregla R, Hetzer R, Regitz-Zagrosek V. (2004)
RNA-binding proteins heterogeneous nuclear ribonucleoprotein A1, E1, and K are involved in post-transcriptional control of collagen I and III synthesis.
Circ Res. 95(11):1058-1066.
 Sequences deriving from COL1A1 [1277], COL1A2 [1278], COL3A1 [1281] EMSA, UV cross-linking and immunoprecipitation with HT1080 cytoplasmic extracts or recombinant protein. MALDI-TOF.
hnRNP KAAAAAAA-4Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
8917439Dejgaard K, Leffers H. (1996)
Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains.
Eur J Biochem. 241(2):425-431.
 Synthesized oligos Homopolymer binding assay with recombinant protein.
hnRNP KUUUUUUU-2Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
8917439Dejgaard K, Leffers H. (1996)
Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains.
Eur J Biochem. 241(2):425-431.
 Synthesized oligos Homopolymer binding assay with recombinant protein.
hnRNP KAGCCACCUCCCACCCCACCCCA-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
17928403Lee PT, Liao PC, Chang WC, Tseng JT. (2007)
Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover.
Mol Biol Cell. 18(12):5004-5013.
 Sequences deriving from mutated GAST [2520] and homopolymers. EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts.
hnRNP KCCCAGCCCUGUCCCCUGAAAAACUGA-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
17928403Lee PT, Liao PC, Chang WC, Tseng JT. (2007)
Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover.
Mol Biol Cell. 18(12):5004-5013.
 Sequences deriving from mutated GAST [2520] and homopolymers. EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts.
hnRNP KCCCAGAAAUGUCCCCUGAAAAACUGA-5Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
17928403Lee PT, Liao PC, Chang WC, Tseng JT. (2007)
Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover.
Mol Biol Cell. 18(12):5004-5013.
 Sequences deriving from mutated GAST [2520] and homopolymers. EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts.
hnRNP KAAAAGCCCUGUCAAAUGAAAAACUGA-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
17928403Lee PT, Liao PC, Chang WC, Tseng JT. (2007)
Epidermal growth factor increases the interaction between nucleolin and heterogeneous nuclear ribonucleoprotein K/poly(C) binding protein 1 complex to regulate the gastrin mRNA turnover.
Mol Biol Cell. 18(12):5004-5013.
 Sequences deriving from mutated GAST [2520] and homopolymers. EMSA, UV cross-linking, competition assays, western blot, pull-down assay and mass spectrometry with AGS cytoplasmic extracts.
hnRNP KGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP KAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: HNRNPK, heterogeneous nuclear ribonucleoprotein K, CSBP, TUNP, HNRPK, FLJ41122
hnRNP K carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9218800).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP LCACACCACAACGGCACCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACACACCCACACCUCGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACCCACCCACAUACAUACAU-10Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
9880507Shih SC, Claffey KP. (1999)
Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L.
J Biol Chem. 274(3): 1359-1365.
 Sequence of partial VEGFC [7424] 3'UTR UV crosslink with M21 cell cytoplasmic extracts. Western blot, immunoprecipitation, antisense RNAs.
hnRNP LCACCCCAAACAACAACCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUGCAAAUGAAAUGACUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCUACAUCACUACAUUCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUGCAGCCCUCGUCAUUGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAGGCAAUACGCACACCACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACACACAGACAGACACACACACACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
19298794Lee DH, Lim MH, Youn DY, Jung SE, Ahn YS, Tsujimoto Y, Lee JH. (2009)
hnRNP L binds to CA repeats in the 3'UTR of bcl-2 mRNA.
Biochem Biophys Res Commun. 382(3): 583-587.
 Synthesized sequences. UV cross-linking assay and super-shift R-EMSA in MCF-7 cytoplasmic extract.
hnRNP LCACACACACACACACACACACACACACACACACACACA-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
12447348Hui J, Stangl K, Lane WS, Bindereif A. (2003)
HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats.
Nat Struct Biol. 10(1): 33-37.
In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences.In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation.UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis.
hnRNP LCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
12447348Hui J, Stangl K, Lane WS, Bindereif A. (2003)
HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats.
Nat Struct Biol. 10(1): 33-37.
In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences.In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation.UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis.
hnRNP LCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA-8Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
12447348Hui J, Stangl K, Lane WS, Bindereif A. (2003)
HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats.
Nat Struct Biol. 10(1): 33-37.
In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences.In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation.UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis.
hnRNP LUCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGCAUUCUCACCCGCAAGCACCUU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
16001081Rothrock CR, House AE, Lynch KW. (2005)
HnRNP L represses exon splicing via a regulated exonic splicing silencer.
EMBO J. 24(15): 2792-2802.
In this context, hnRNP L does not bind to CACUGGAUUCUCACCCGGAAGUA motif. Synthesized sequences. RNA affinity purification, mass spectrometry, immunblotting, UV-crosslink in JSL1 nuclear extract.
hnRNP LCACUGGAUUCUCACCCGGAAGUA-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP LGACACACACGCCUGACUCAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUCCACUUUCAAGUGACCCCUUACCUACUCACACCACUGGAUUCUCACCCGGAAGUACCUU-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP LCACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
hnRNP LCACUGCAGUGUCACCCGCAAGCA-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18719244Topp JD, Jackson J, Melton AA, Lynch KW. (2008)
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
RNA. 14(10): 2038-2049.
 Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vivo splicing in JSL1 cells.UV cross-linking and immunoprecipitation in JSL1 nuclear extract.
hnRNP LCACUGCUUUCUCACCCGCUAGCG-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18719244Topp JD, Jackson J, Melton AA, Lynch KW. (2008)
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
RNA. 14(10): 2038-2049.
 Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vivo splicing in JSL1 cells.UV cross-linking and immunoprecipitation in JSL1 nuclear extract.
hnRNP LCCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP LCAGCCACCUCUCCCCUCUCCGCACUGCUGCCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP LUACCACCACCCCUUCCCCAUUCCCUUGCCCUGCU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP LCUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11867641Expert-Bezancon A, Le Caer JP, Marie J. (2002)
Heterogeneous nuclear ribonucleoprotein (hnRNP) K is a component of an intronic splicing enhancer complex that activates the splicing of the alternative exon 6A from chicken beta-tropomyosin pre-mRNA.
J Biol Chem. 277(19):16614-16623.
 Synthesized sequences. Construct of chicken TPM3 [396430] EX6A - INT6 - EX7In vitro splicing in HeLa NEProtein affinity purification with HeLa NE. Mass spectrometry and Western blots. Two-dimensional gel analysis.
hnRNP LCGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP LCGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
hnRNP LCAGAAGGGGAGGGGUUCCA-6Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP LAACAAGGGGUGGGGGAAAA-6Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
17567613Wang E, Dimova N, Cambi F. (2007)
PLP/DM20 ratio is regulated by hnRNPH and F and a novel G-rich enhancer in oligodendrocytes.
Nucleic Acids Res. 35(12):4164-4178.
 Sequences deriving from PLP1 [5354]. Constructs of PLP1 [5354] INT3 - EX3A - E3B - INT4.In vivo splicing in oligodendroglial precursors.RNA-affinity precipitations of oligodendroglial precursor NE, mass spectroscopy, western blot. siRNA
hnRNP LCUGCACCACCACCUGGUUC-3Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
hnRNP LCUGCACCACCACCUAUCUA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
hnRNP LUGUGUCACUGUCUGGUUC-1Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
hnRNP LGGCAGGAAGAAGAGGAGCA-3Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
hnRNP LCCAGACACCGGAAACCCCUGCCACACCAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
hnRNP LUAUGAUAGGGACUUAGGGUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUAUGACCCGGACUCCCGGUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUAUGAUAGGCUCAUAGGGUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUAUGAUAGGCACUUAGGCUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUUAGAUCGAUGGGAAAAAAUUCGUUAAGG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUUAGAUCGAUCCCAAAAAAUUCGUUAAGG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUAAAUGUGGGGACCUAGAGGAGGAGCUGAAAAUUGUUA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUAAACUACGCGACCUAGAGGAGGAGCUGAAAAUUGUUA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LUGCAAUGUACUUGCAAACAAUGGCCUGAGUGUGCAAAGAAAAUGUCUGCUAACUGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
hnRNP LACAUGAACCUUCAGCGGCCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGUGCCCCAAACACCUUGCAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUCGCUGACAUACACAUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACAAUUGCACGCGACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGGAACGGCAUACUGUUGGCU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAACACACACAACGCCAUCUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGUCGGACACCAGCUCUGUGUA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCUCAUGUCCACACAUGCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUGGCCUAACACAUGAUCAGA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACACACCCACAACCCCC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUACACACACAACACAUUGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACUACACACGACCAUGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACAGCACACAACCUGGCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCACCAGCUCAUACACUACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAAACCACCCCAAACAACAAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUUACACCACACCCACUCGG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUAACACCACCCAUUGCACCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACGACAUACCAUACAGAU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUCACACGACACUACCGGCAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAAAAACACCACCACAAACAA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUGUAAUUGUUCUCCGCGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAAUCACAUACGCUUUACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCUGCACCCUCCACCACGCCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUACACACAUUCCGUGCAUGG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACUGAUGACACCACUGACAA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUCACACCACUUAAGCAAACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCGCACCACAUUACAUGCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCCGGACUACACGCAACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCGCUGCACCCACAGCCACG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCGCUGCCCUCACUCGCACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGGGGACUCUACAUCGACAUA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCAACCCCAAACCCCAACAA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUCACUAAACUCCACAGCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAACACGCACGCAUUGUUACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACGACACGACGACACAU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCCUGACACAUACACCGACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUGCAAACCUAAAUGAGGUACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUUUACAUACCAACACCAGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUACAUGACACACACACGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGUGCCAUGAACACCACGACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACUACAUAAGCACCACGA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACACACUACACCACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACAUUGCAUACAUACACGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGAUCGCGCCCGCUGCAGUCG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGUCCCUAGUAGUGUGGGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACAUUGCACACAUACCCC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAGUAACACACACCCACGGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGAACACGACAUACAUCGCAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUGCAUCUACGUACACACACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUGCACACCCACAUGUCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCCCCCACCACCACAACCCC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAAACACAACACAACCACCCC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGGUAGGACCGGUGCUGUGAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGACACACACAACACAGACGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCAAACAGUACACCAGCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACAAGCGCUCCCCGGCUCAU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGUACAUAUACACACACACGA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUUCACCUUUACCGGCAGCCU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACCGGUGUACACUACAUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCAAACACGCACCAACACACC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCACACGAAUAACAUCGCAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCUACACACGACACAUGAGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCGGCCCACACGCAUGGUCGG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAAACACCACAACACACACCC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUACACUAACAACGCACACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACGCAACCAUGCAUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCCCGCACUGACCGCACGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGACCUAUACACCGCACCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACAUACACCACACGCAUUGA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUUCACACCCCGCCGUUGACC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCAUACACACCCGCCUGGCA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACCGACGGCCUAUAUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUACGCACAUAACACAUCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAGCACCUGCACACAUACAUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACCAGCCGGCACACACACG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACAACUCACGGCCACACG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCAAACGGUGACCACUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUGAUUAACUACACGCAAGA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LUCAUGCCCUGACAGUGCACG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACUCACACUACAUAUGGC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAACUACACAUACACCGGACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACAUAAAACACAUACAUG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACAGCAUACAUACAUACACG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGACUAGCAACACACACACAG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAGACACACACCCAGCCGGCC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAAAGGGGCACAGUGUGCAG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LGCACAUAUACAUCAUAACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACGCAUACUUACAUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACAGCAUAACAUAUACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCCGGACAAACACACGAACAC-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACCACACACUGCUCACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LAUACAACACACACGACAACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LACACCGAGACACUCACAACA-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
15889141Hui J, Hung LH, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. (2005)
Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing.
EMBO J. 24(11): 1988-1998.
 Construct and variants of GSTZ1 [2954] EX4 - INT4 - EX5 - INT5 - EX6. Construct and variants of SLC2A2 [6514] EX3 - INT3 - EX4 - INT4 - EX5. Construct and variants of RFXANK [8625] EX4 - INT4 - EX5 - INT5 - EX6.In vitro splicing with HeLa nuclear extracts.Pull-down assays, UV crosslink, immunoprecipitation with HeLa nuclear extracts. SELEX of 20nt random with recombinant protein.
hnRNP LCACACCACUGCAUUCUCACCCGCAAGCACC-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LUACAGCCAGCACCUUUCCUACA-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LUAGAGCCAGCAGCUUUCCUAGA-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LCACACCACA-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LCACCUCCAACACCACCAUCACAGCGAACACC-2Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LCAGCUCCAAGACGACCAUCAGAGCGAAGACC-1Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LCCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP LCCACGCACGCAGACUCGCAG-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP LCACGCAGACAUUCAGCGGCU-5Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP LGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP LAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: HNRNPL, heterogeneous nuclear ribonucleoprotein L, HNRPL, FLJ35509, P/OKcl.14
Note that "short distances of the CA-sequence to the alternative 5'ss correlate with a silencer role (SLC2A2, RFXANK), longer distances with enhancer function (MAPK10, GSTZ1, eNOS)" (PMID:15889141).
Excess hnRNP L activates exon 6A inclusion in its own mRNA and, via NMD, contributes to homeostasis of hnRNP L levels (PMID: 19124611).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP LLCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA-5Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
19124611Rossbach O, Hung LH, Schreiner S, Grishina I, Heiner M, Hui J, Bindereif A. (2009)
Auto- and cross-regulation of the hnRNP L proteins by alternative splicing.
Mol Cell Biol. 29(6): 1442-1451.
In this context, hnRNP L activates inclusion of exon 6A. Construct of hnRNPL [3191] EX1_DUP - INT1_DUP - INT6_hnRNPL - EX6A_hnRNPL - INT6_hnRNPL - INT2_DUP - EX2_DUP. Construct of hnRNPLL [92906] EX6 - INT6 - EX7.In vitro alternative splicing assay and hnRNP L depletion/complementation and RNAi knockdown in HeLa cell in order to determine hnRNP L activity.Pull-down assay and immunoblotting in HeLa cell nuclear extract.
hnRNP LLCACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
18719244Topp JD, Jackson J, Melton AA, Lynch KW. (2008)
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
RNA. 14(10): 2038-2049.
 Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vivo splicing in JSL1 cells.UV cross-linking and immunoprecipitation in JSL1 nuclear extract.
hnRNP LLCACUGGAUUCUCACCCGGAAGUA-2Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
18719244Topp JD, Jackson J, Melton AA, Lynch KW. (2008)
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
RNA. 14(10): 2038-2049.
 Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vivo splicing in JSL1 cells.UV cross-linking and immunoprecipitation in JSL1 nuclear extract.
hnRNP LLCACUGCAGUGUCACCCGCAAGCA-2Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
18719244Topp JD, Jackson J, Melton AA, Lynch KW. (2008)
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
RNA. 14(10): 2038-2049.
 Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vivo splicing in JSL1 cells.UV cross-linking and immunoprecipitation in JSL1 nuclear extract.
hnRNP LLCACACCACUGCAUUCUCACCCGCAAGCACC-10Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLUACAGCCAGCACCUUUCCUACA-10Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLUAGAGCCAGCAGCUUUCCUAGA-10Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLCACACCACA-10Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLCAGACGACA-4Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLCACCUCCAACACCACCAUCACAGCGAACACC-10Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLCAGCUCCAAGACGACCAUCAGAGCGAAGACC-4Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
22402488Preubner M, Schreiner S, Hung LH, Porstner M, Jack HM, Benes V, Ratsch G, Bindereif A. (2012)
HnRNP L and L-like cooperate in multiple-exon regulation of CD45 alternative splicing.
Nucleic Acids Res. 40(12):5666-5678.
 Construncts of PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa and siRNA knockdown.Mutational analysis, pulldown, western blot in HeLa NE
hnRNP LLCCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU-1Gene Name and Synonymous: HNRPLL, heterogeneous nuclear ribonucleoprotein L-like, SRRF, hnRNPLL, HNRPLL.
hnRNP LL causes CD45 EX4 skipping via ESS1 binding (PMID: 18719244).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
hnRNP MGGGGGGG-7Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. 3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP MGAAGGAA5Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. 24533984Cho S, Moon H, Loh TJ, Oh HK, Cho S, Choy HE, Song WK, Chun JS, Zheng X, Shen H. (2014)
hnRNP M facilitates exon 7 inclusion of SMN2 pre-mRNA in spinal muscular atrophy by targeting an enhancer on exon 7.
Biochim Biophys Acta. 1839(4):306-315.
 Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8In vivo splicing in 293A, C33A, SH-SY5Y, GM03813RNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis
hnRNP MGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP MAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPM, heterogeneous nuclear ribonucleoprotein M, HTGR1, NAGR1, HNRPM4, HNRNPM4, DKFZp547H118. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP P (TLS)AAAAAAA-7Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP P (TLS)GGGGGGG-7Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 3386636Swanson MS, Dreyfuss G. (1988)
Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
Mol Cell Biol. 8(5):2237-2241.
 homopolymers Immunoblotting of proteins selected by affinity chromatography with ribonucleotide homopolymer and HeLa nuclear extract.
hnRNP P (TLS)UUUUUUU-7Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)CGGUGG-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)GGGUGA-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)GGGUGC-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)GGCGAAUUCGCUGGGGCUGGGCAGAGCGCGCAGGGUUGAGGGGAGCAGGGUCCUUCACUGGGGUGAA-5Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)UUAGGGUUAGGGUUAGGGUUAGGG-10Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 18776329Takahama K, Kino K, Arai S, Kurokawa R, Oyoshi T. (2008)
Identification of RNA binding specificity for the TET-family proteins.
Nucleic Acids Symp Ser (Oxf). (52):213-214.
 Synthesized oligos EMSA with recombinant protein
hnRNP P (TLS)UUGGGGUUGGGGUUGGGGUUGGGG-1Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 18776329Takahama K, Kino K, Arai S, Kurokawa R, Oyoshi T. (2008)
Identification of RNA binding specificity for the TET-family proteins.
Nucleic Acids Symp Ser (Oxf). (52):213-214.
 Synthesized oligos EMSA with recombinant protein
hnRNP P (TLS)GGGUGG-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)GGGUGU-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)UGGUGA-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)UGGUGG-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)UGGUGU-8Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)CGGUGA-2Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 11098054Lerga A, Hallier M, Delva L, Orvain C, Gallais I, Marie J, Moreau-Gachelin F. (2001)
Identification of an RNA binding specificity for the potential splicing factor TLS.
J Biol Chem. 276(9):6807-6816.
 Homopolymers. Sequences of 25 nt random for SELEX. SELEX of 25nt random with recombinant protein. SDS-PAGE, EMSA, UV crosslink, competition and immunoprecipitation assays with HeLa nuclear extracts.
hnRNP P (TLS)GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP P (TLS)AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP P (TLS)AGGGUUGAGGGGAGCAGGG-5Gene Name and Synonymous: FUS, fusion (involved in t(12,16) in malignant liposarcoma), hnRNP-P2, TLS, CHOP, FUS1, FUS-CHOP, TLS/CHOP. 7567462Sirand-Pugnet P, Durosay P, Brody E, Marie J. (1997)
An intronic (A/U)GGG repeat enhances the splicing of an alternative intron of the chicken beta-tropomyosin pre-mRNA.
Nucleic Acids Res. 23(17):3501-3507.
 Sequences deriving from chicken TPM2 [396430]  UV crosslink and immunoprecipitation in HeLa nuclear extract
hnRNP QAAAAAGGAAAAAAAAAAACAAAAGACAAAAAAAAAAUAAGC-5Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 18045242Duning K, Buck F, Barnekow A, Kremerskothen J. (2008) SYNCRIP, a component of dendritically localized mRNPs, binds to the translation regulator BC200 RNA. J Neurochem. 105(2):351-359.  Sequences deriving from BCYRN1 [618] and homopolymers. RNA af?nity puri?cation assays, Western blot, EMSA with RNA competitors using recombinant protein
hnRNP QAAAAAAA-5Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 18045242Duning K, Buck F, Barnekow A, Kremerskothen J. (2008) SYNCRIP, a component of dendritically localized mRNPs, binds to the translation regulator BC200 RNA. J Neurochem. 105(2):351-359.  Sequences deriving from BCYRN1 [618] and homopolymers. RNA af?nity puri?cation assays, Western blot, EMSA with RNA competitors using recombinant protein
hnRNP QUUUUUUU-1Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 15340051Kim JH, Paek KY, Ha SH, Cho S, Choi K, Kim CS, Ryu SH, Jang SK. (2004)
A cellular RNA-binding protein enhances internal ribosomal entry site-dependent translation through an interaction downstream of the hepatitis C virus polyprotein initiation codon.
Mol Cell Biol. 24(18):7878-7890.
 Sequences deriving from HCV2_gp1 [11027172] and homopolymers. Protein affinity purification, MALDI-TOF, UV cross-linking and mutation analysis with HeLa S10 extracts. Competition assay using homopolymers with purified protein
hnRNP QAUGAGCACAAAUCCUAAACCUCAAAGAAAAACC-5Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 15340051Kim JH, Paek KY, Ha SH, Cho S, Choi K, Kim CS, Ryu SH, Jang SK. (2004)
A cellular RNA-binding protein enhances internal ribosomal entry site-dependent translation through an interaction downstream of the hepatitis C virus polyprotein initiation codon.
Mol Cell Biol. 24(18):7878-7890.
 Sequences deriving from HCV2_gp1 [11027172] and homopolymers. Protein affinity purification, MALDI-TOF, UV cross-linking and mutation analysis with HeLa S10 extracts. Competition assay using homopolymers with purified protein
hnRNP QAUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG5Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP QAUUUUCCUUACAGGGUUUCAGACAAAAUCAAAAAG5Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP QAUUUUCCUUACAGGGCUUUUUGAUUUUGUCUAAAA5Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
hnRNP QGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP QAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: SYNCRIP, synaptotagmin binding cytoplasmic RNA interacting protein, PP68, NSAP1, GRYRBP, HNRPQ1, GRY-RBP, hnRNP-Q, FLJ31626, dJ3J17.2 RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP UGGGGGGG-4Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 1628625Kiledjian M, Dreyfuss G. (1992)
Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box.
EMBO J. 11(7):2655-64.
hnRNP U has no affinity to poly(rC). homopolymers SDS-PAGE of ribonucleotide homopolymers with recombinant protein
hnRNP UAAAAAAA-7Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 1628625Kiledjian M, Dreyfuss G. (1992)
Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box.
EMBO J. 11(7):2655-64.
hnRNP U has no affinity to poly(rC). homopolymers SDS-PAGE of ribonucleotide homopolymers with recombinant protein
hnRNP UUUUUUUU-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 1628625Kiledjian M, Dreyfuss G. (1992)
Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box.
EMBO J. 11(7):2655-64.
hnRNP U has no affinity to poly(rC). homopolymers SDS-PAGE of ribonucleotide homopolymers with recombinant protein
hnRNP UACGAAGACAAACAAA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 9765391Gupta AK, Drazba JA, Banerjee AK. (1998)
Specific interaction of heterogeneous nuclear ribonucleoprotein particle U with the leader RNA sequence of vesicular stomatitis virus.
J Virol. 72(11):8532-8540.
hnRNP U has no affinity to poly(rA). Leader-sense (LS) RNA of Vesicular stomatitis virus (VSV).  EMSA, UV crosslink, immunoprecipitation using HeLa cell nuclear extracts. UV crosslink, competition assay with purified protein.
hnRNP UCCCCCCC-4Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 8174554Fackelmayer FO, Dahm K, Renz A, Ramsperger U, Richter A. (1994)
Nucleic-acid-binding properties of hnRNP-U/SAF-A, a nuclear-matrix protein which binds DNA and RNA in vivo and in vitro.
Eur J Biochem. 221(2):749-757.
hnRNP U has no affinity to poly(rU). homopolymers Filter Binding Assay with purified protein.
hnRNP UUUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UUUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UUUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UUUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UUUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UUUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UCUGAUUUGUAUUUAUUAGACUC-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UAACAGAAAAAGAAAUAUUU-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UCUGAUUUGUACCUAUUAGAUUC-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UUACUGAAGAACAAGUAUUU-5Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
hnRNP UGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
hnRNP UAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: HNRNPU, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), HNRPU, SAF-A, U21.1. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
HTra2alphaAGAAGAACGAGGAACACAA4Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2alphaAAGAA7Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2alphaAAGAAGAA8Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2alphaAAGAAGAAGAA10Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2alphaGACAGAAGAACUCUCGACAGAAGAACUCUCGACAGAAGAACU5Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2alphaGAAGAGGAAG5Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 12403832Seong JY, Han J, Park S, Wuttke W, Jarry H, Kim K. (2002)
Exonic splicing enhancer-dependent splicing of the gonadotropin-releasing hormone premessenger ribonucleic acid is mediated by tra2alpha, a 40-kilodalton serine/arginine-rich protein.
Mol Endocrinol. 16(11):2426-2438.
 Construct of mouse Gnrh1[14714] EX1 - INT1 - EX2 - EX3 - EX4In vitro splicing of of Gnrh1-derived construct in HeLa nuclear extract with different concentrations of the protein.EMSA, UV-crosslink, immunoprecipitation, mutation assay
HTra2alphaGAAAGUCUGAUUGAAGAGGAAGCCAGGCAGAAGAAGA5Gene Name and Synonymous: TRA2A, transformer-2 alpha, HSU53209. 16249178Park E, Han J, Son GH, Lee MS, Chung S, Park SH, Park K, Lee KH, Choi S, Seong JY, Kim K. (2006)
Cooperative actions of Tra2alpha with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript.
J Biol Chem. 281(1):401-409.
 Sequences deriving from mouse Gnrh1 [14714]. Construct of Gnrh1 [14714] EX1 - INT1 - EX2 - EX3 - EX4.In vitro splicing in HeLa NEEMSA, UV cross-linking with recombinant protein
HTra2beta1AGAAGGAAGG3Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 10931943Hofmann Y, Lorson CLL, Stamm S, Androphy EJ, Wirth B. (2000)
Htra2-beta 1 stimulates an exonic splicing enhancer and can restore full-length SMN expression to survival motor neuron 2 (SMN2).
Proc Natl Acad Sci U S A. 97(17): 9618-9623.
 Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 . Sequences and mutants of SMN1 and SMN2 EX7.In vivo splicing in HEK293.UV crosslink, competition assay in HeLa S100 and nuclear extracts. Immunoblotting in C33a extracts.
HTra2beta1GAAAGAAG5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 14709600Stoilov P, Daoud R, Nayler O, Stamm S. (2004)
Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA.
Hum Mol Genet. 13(5): 509-524.
Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. Pull down assay from HEK293 nuclear extract, western blot.
HTra2beta1AGAAGAACGAGGAACACAA4Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2beta1AAGAA7Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2beta1AAGAAGAA8Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2beta1AAGAAGAAGAA10Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2beta1AAUAAGAAG5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 12649279Jiang Z, Tang H, Havlioglu N, Zhang X, Stamm S, Yan R, Wu JY. (2003)
Mutations in tau gene exon 10 associated with FTDP-17 alter the activity of an exonic splicing enhancer to interact with Tra2 beta.
J Biol Chem. 278(21):18997-19007.
 Construct of Tau MAPT [4137] EX10 - INT10 - EX11.In vitro splicing with HeLa, HEK293 and neuroblastoma N2a nuclear extracts.UV crosslink, Western blot, immunoprecipitation, RNA interference with HeLa and HEK293 nuclear extracts.
HTra2beta1AAGAAG5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 16943417Wu JY, Kar A, Kuo D, Yu B, Havlioglu N. (2006)
SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.
Mol Cell Biol. 26(18):6739-6747.
 Construct of Tau MAPT [4137] EX9 - INT9 - EX10 - INT10 - EX11 with GFP cDNA inserted into EX11.In vivo splicing in HEK293.Positive clones identified by fluorescence-activated cell sorting and visual inspection. Confirmed by UV crosslink, immunoprecipitation, SDS-PAGE.
HTra2beta1GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
HTra2beta1GACAGAAGAACUCUCGACAGAAGAACUCUCGACAGAAGAACU5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 9546399Tacke R, Tohyama M, Ogawa S, Manley JL. (1998)
Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing.
Cell. 93(1): 139-148.
 Sequences of 20 nt random for SELEX. Sequence of beta-globin [3043] and constructs of murine IgM-based pre-mRNA for in vitro splicing. In vitro splicing in HeLa S100 and nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by EMSA in HeLa nuclear extract and S100. EMSA with recombinant protein.
HTra2beta1AAGAAGAAG9Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 12649279Jiang Z, Tang H, Havlioglu N, Zhang X, Stamm S, Yan R, Wu JY. (2003)
Mutations in tau gene exon 10 associated with FTDP-17 alter the activity of an exonic splicing enhancer to interact with Tra2 beta.
J Biol Chem. 278(21):18997-19007.
 Construct of Tau MAPT [4137] EX10 - INT10 - EX11.In vitro splicing with HeLa, HEK293 and neuroblastoma N2a nuclear extracts.UV crosslink, Western blot, immunoprecipitation, RNA interference with HeLa and HEK293 nuclear extracts.
HTra2beta1GAAGAAGGAAA5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 14709600Stoilov P, Daoud R, Nayler O, Stamm S. (2004)
Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA.
Hum Mol Genet. 13(5): 509-524.
Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. Pull down assay from HEK293 nuclear extract, western blot.
HTra2beta1GAAAGAAU5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 14709600Stoilov P, Daoud R, Nayler O, Stamm S. (2004)
Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA.
Hum Mol Genet. 13(5): 509-524.
Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. Pull down assay from HEK293 nuclear extract, western blot.
HTra2beta1GAAGAAUGAAGAAA5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 14709600Stoilov P, Daoud R, Nayler O, Stamm S. (2004)
Human tra2-beta1 autoregulates its protein concentration by influencing alternative splicing of its pre-mRNA.
Hum Mol Genet. 13(5): 509-524.
Hyperphosphorylation of HTra2beta1 strongly reduces its binding to RNA. Construct of tra2-beta [6434 ] EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4. Pull down assay from HEK293 nuclear extract, western blot.
HTra2beta1GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
HTra2beta1GAAGAAGAACGAAGAAGAAC5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
HTra2beta1GGCGACUGGGGGGUCAGGG5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 17913700Novoyatleva T, Heinrich B, Tang Y, Benderska N, Butchbach ME, Lorson CL, Lorson MA, Ben-Dov C, Fehlbaum P, Bracco L, Burghes AH, Bollen M, Stamm S.(2008)
Protein phosphatase 1 binds to the RNA recognition motif of several splicing factors and regulates alternative pre-mRNA processing.
Hum Mol Genet. 17(1):52-70.
 Synthesized sequences EMSA with recombinant protein
HTra2beta1CAAAAAGAAGGAAGGUGCUCACAU10Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 19953646Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010)
A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy.
Hum Mutat. 31(1):E1110-1125.
 Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein
HTra2beta1CAAAAAGAACGAAGGUGCUCACAU10Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 19953646Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010)
A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy.
Hum Mutat. 31(1):E1110-1125.
 Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein
HTra2beta1GAAGAA10Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 20926394Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y. (2010)
Structural basis for the dual RNA-recognition modes of human Tra2-ß RRM.
Nucleic Acids Res. 39(4):1538-1553.
 Synthesized sequences NMR spectroscopy
HTra2beta1GACUUCAACAAGUC9Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 20926394Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y. (2010)
Structural basis for the dual RNA-recognition modes of human Tra2-ß RRM.
Nucleic Acids Res. 39(4):1538-1553.
 Synthesized sequences NMR spectroscopy
HTra2beta1UCAAC1Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 20926394Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Güntert P, Yokoyama S, Muto Y. (2010)
Structural basis for the dual RNA-recognition modes of human Tra2-ß RRM.
Nucleic Acids Res. 39(4):1538-1553.
 Synthesized sequences NMR spectroscopy
HTra2beta1AAGAAC5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 24692659Moursy A, Allain FH, Clery A. (2014)
Characterization of the RNA recognition mode of hnRNP G extends its role in SMN2 splicing regulation.
Nucleic Acids Res.
 Sequences deriving from SMN2 [6607]. Synthetic sequences. NMR spectroscopy, isothermal titration calorimetry. EMSA with recombinat protein and other competitor protein (HTra2beta1).
HTra2beta1GAAGGA5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 24632473Cho S, Moon H, Loh TJ, Oh HK, Williams DR, Liao DJ, Zhou J, Green MR, Zheng X, Shen H. (2014)
PSF contacts exon 7 of SMN2 pre-mRNA to promote exon 7 inclusion.
Biochim Biophys Acta.
 Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8In vivo splicing in 293A, C33A, SH-SY5Y along with PSF expression vectorRNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis.
HTra2beta1GAAUUA5Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 24632473Cho S, Moon H, Loh TJ, Oh HK, Williams DR, Liao DJ, Zhou J, Green MR, Zheng X, Shen H. (2014)
PSF contacts exon 7 of SMN2 pre-mRNA to promote exon 7 inclusion.
Biochim Biophys Acta.
 Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8In vivo splicing in 293A, C33A, SH-SY5Y along with PSF expression vectorRNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis.
HTra2beta1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC2Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
HTra2beta1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC2Gene Name and Synonymous: SFRS10, splicing factor arginine/serine-rich 10 (transformer 2 homolog, Drosophila), TRA2B, SRFS10, TRAN2B, TRA2-BETA, Htra2-beta, DKFZp686F18120. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
HuBUUUUUUAUUUU-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 17035636Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006)
A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators.
Mol Biol Cell. 17(12):5105-5114.
 Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts.
HuBAUUUAUUUU-10Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAUUUA-10Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAUUUU-10Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAAUUU-7Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAUUGU-7Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBAUUUACUUU-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUAAUUUU-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUCAUUUU-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBAUUUAUACU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBAUUUAUAUA-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBCUUUAAUUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBCUUUCUUUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUAUUAUUUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUCUUAUAUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUAUACUUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUAUAUUUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUGUAUUGU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUAAUUUG-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUGAUUUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAAGUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAGUUA-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAUGUU-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAUUGA-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBUUUUAUUUG-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 7972035Gao FB, Carson CC, Levine T, Keene JD. (1994)
Selection of a subset of mRNAs from combinatorial 3' untranslated region libraries using neuronal RNA-binding protein Hel-N1.
Proc Natl Acad Sci U S A. 91(23):11207-11211.
 Sequences of 25nt random for SELEX. SELEX of random 25nt with purified protein.
HuBCUUUUU-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBCUUUUA-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBCUUUUUA-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBCUUUC-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBCUUUA-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBCUUUUUG-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUA-10Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUG-9Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUC-3Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUUUC-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUUUA-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUUC-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBAUUUUUUUA-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBGUUUC-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBGUUUUUA-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBGUUUUC-2Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBGUUUA-1Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 8497264Levine TD, Gao F, King PH, Andrews LG, Keene JD. (1993)
Hel-N1: an autoimmune RNA-binding protein with specificity for 3' uridylate-rich untranslated regions of growth factor mRNAs.
Mol Cell Biol. 13(6):3494-3504.
 Sequences of 25 nt random for SELEX. SELEX of 25nt random sequences with recombinant protein.
HuBUUUUUUUUUUUUU-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuBAUUUAUUUAUUUA-5Gene Name and Synonymous: ELAVL2, ELAV (embryonic lethal abnormal vision Drosophila)-like 2 (Hu antigen B), HELN1, HEL-N1. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuCAUUUAUCUACUUUCUG-5Gene Name and Synonymous: ELAVL3, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C), HUC, HUCL, PLE21, MGC20653, DKFZp547J036 10079173Sakai K, Kitagawa Y, Hirose G. (1999)
Analysis of the RNA recognition motifs of human neuronal ELAV-like proteins in binding to a cytokine mRNA.
Biochem Biophys Res Commun. 256(2):263-268.
 Synthesized sequences EMSA with recombinant protein
HuCUUUUUUU-5Gene Name and Synonymous: ELAVL3, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C), HUC, HUCL, PLE21, MGC20653, DKFZp547J036 10710437King PH. (2000)
RNA-binding analyses of HuC and HuD with the VEGF and c-myc 3'-untranslated regions using a novel ELISA-based assay.
Nucleic Acids Res. 28(7):E20.
 Homopolymers ELISA and competition assays with recombinant protein
HuDUUUUUUAUUUU-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 17035636Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006)
A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators.
Mol Biol Cell. 17(12):5105-5114.
 Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts.
HuDAUAUUUAUAUUUUUAUUUUAUUUUUUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 8626712Chung S, Jiang L, Cheng S, Furneaux H. (1996)
Purification and properties of HuD, a neuronal RNA-binding protein.
J Biol Chem. 271(19):11518-11524.
 WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR EMSA, Filter Binding Assay, RNase Protection Assay.
HuDAUACGUAUAUUUUUAUUUUAUUUUUUU-8Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 8626712Chung S, Jiang L, Cheng S, Furneaux H. (1996)
Purification and properties of HuD, a neuronal RNA-binding protein.
J Biol Chem. 271(19):11518-11524.
 WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR EMSA, Filter Binding Assay, RNase Protection Assay.
HuDAUAUUUAUAUCGCUAUUUUAUUUUUUU-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 8626712Chung S, Jiang L, Cheng S, Furneaux H. (1996)
Purification and properties of HuD, a neuronal RNA-binding protein.
J Biol Chem. 271(19):11518-11524.
 WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR EMSA, Filter Binding Assay, RNase Protection Assay.
HuDAUAUUUAUAUUUUUAUGCUAUUUUUUU-6Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 8626712Chung S, Jiang L, Cheng S, Furneaux H. (1996)
Purification and properties of HuD, a neuronal RNA-binding protein.
J Biol Chem. 271(19):11518-11524.
 WT and mutants A/U rich elements (ARE) of c-fos [2353] 3'UTR EMSA, Filter Binding Assay, RNase Protection Assay.
HuDUCCACUUUCCUCUCUAUUUCUCUCUG-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 9045688Chung S, Eckrich M, Perrone-Bizzozero N, Kohn DT, Furneaux H. (1997)
The Elav-like proteins bind to a conserved regulatory element in the 3'-untranslated region of GAP-43 mRNA.
J Biol Chem. 272(10):6593-6598.
 Sequences of human GAP43 [2596] Filter binding assay, competition assay with recombinant protein
HuDUCUUAAUUAUUAUUUGUGUUUUAAUUUAAACACCUCCUCAUG-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 9685407Joseph B, Orlian M, Furneaux H. (1998)
p21(waf1) mRNA contains a conserved element in its 3'-untranslated region that is bound by the Elav-like mRNA-stabilizing proteins.
J Biol Chem. 273(32):20511-20516.
 Sequence deriving from CDKN1A [1026] and FOS [2353] RNase T1 selection, nitrocellulose filter binding assay, EMSA and competition assay with recombinant protein
HuDUUAUUUAUUUAUUUAUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 10848602Park S, Myszka DG, Yu M, Littler SJ, Laird-Offringa IA. (2000)
HuD RNA recognition motifs play distinct roles in the formation of a stable complex with AU-rich RNA.
Mol Cell Biol. 20(13):4765-4772.
 Synthesized sequences EMSA with recombinant protein
HuDUUAUUUAUUUAUU-4Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 10848602Park S, Myszka DG, Yu M, Littler SJ, Laird-Offringa IA. (2000)
HuD RNA recognition motifs play distinct roles in the formation of a stable complex with AU-rich RNA.
Mol Cell Biol. 20(13):4765-4772.
 Synthesized sequences EMSA with recombinant protein
HuDUUAUUUAUUU-3Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 10848602Park S, Myszka DG, Yu M, Littler SJ, Laird-Offringa IA. (2000)
HuD RNA recognition motifs play distinct roles in the formation of a stable complex with AU-rich RNA.
Mol Cell Biol. 20(13):4765-4772.
 Synthesized sequences EMSA with recombinant protein
HuDUUUUUUU-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12384599Kasashima K, Sakashita E, Saito K, Sakamoto H. (2002)
Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins.
Nucleic Acids Res. 30(20):4519-4526.
 Homopolymers Homopolymer binding assay with HeLa cell extracts
HuDAAAAAAA-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12384599Kasashima K, Sakashita E, Saito K, Sakamoto H. (2002)
Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins.
Nucleic Acids Res. 30(20):4519-4526.
 Homopolymers Homopolymer binding assay with HeLa cell extracts
HuDCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUUUUUUUUU-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12605686Wein G, Rossler M, Klug R, Herget T. (2003)
The 3'-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR.
Eur J Biochem. 270(2):350-365.
 Sequences deriving from mouse Marcks [17118] EMSA, UV cross-linking with recombinant protein
HuDUUAUUUAUUUAUUUAUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUAUUUAUUUAUUUAU-9Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUAUUUAUUUAUUUA-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDAUUUAUUUAUUUAU-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDAUUUAUUUAUUUA-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUAUUUAUUUA-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDAUUUAUUUAUUU-6Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUAUUUAUUU-4Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUAUUUAUUUAUUUAUUUAUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUAUUUAUUUAUUUAUUUAUUUAUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUAUUUAUUUAUUUAUUUAUUUAUUUAUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUAUUUAUUUAUUUAUUUAUUUAUUUAUUUAUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDAUUUUUUUUUUUA-8Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUUAUUUAUUUU-8Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUUUUUUUUUUU-10Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDAUUUCAUUAUUUA-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUGUUUCCUUUUAU-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUAUUUACUAAU-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUGGAUUGUAUUCA-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUAUUUCCUUUAU-7Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUGUAUCUAAAU-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUGCGUGCUUGCCU-1Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUUAUUGCCUAA-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDCUUGUUUGCUGUU-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDCUACUGGCACGCA-1Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDCCUACUUGUUUCU-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDGUUGUUUGUUCUA-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDGUUUUUUUCCUAC-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDGUUUUUAUAUCCA-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDAGGUUUGUGUCAG-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDCUUUUGUUCUAGC-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDCUUGAAUUUCUAC-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDUUUGCAUUUUAUC-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuDGUCUUGACUUUUC-5Gene Name and Synonymous: ELAVL4, ELAV (embryonic lethal abnormal vision Drosophila)-like 4 (Hu antigen D), PNEM. 12900401Park-Lee S, Kim S, Laird-Offringa IA. (2003)
Characterization of the interaction between neuronal RNA-binding protein HuD and AU-rich RNA.
J Biol Chem. 278(41):39801-39808.
 Synthesized sequences. Sequences of 13nt random for SELEX. SELEX of random 13nt and EMSA with recombinant protein.
HuRAUUUA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9882309Sokolowski M, Furneaux H, Schwartz S. (1999)
The inhibitory activity of the AU-rich RNA element in the human papillomavirus type 1 late 3' untranslated region correlates with its affinity for the elav-like HuR protein.
J Virol. 73(2):1080-1091.
HuR has no affinity to poly(A), poly(G) and poly(C). Sequence and mutants of human Papillomavirus Type 1 Late 3' UTR h1ARE. UV crosslink, immunoblotting with HeLa nuclear extracts, EMSA with recombinant protein.
HuRUUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9882309Sokolowski M, Furneaux H, Schwartz S. (1999)
The inhibitory activity of the AU-rich RNA element in the human papillomavirus type 1 late 3' untranslated region correlates with its affinity for the elav-like HuR protein.
J Virol. 73(2):1080-1091.
HuR has no affinity to poly(A), poly(G) and poly(C). Sequence and mutants of human Papillomavirus Type 1 Late 3' UTR h1ARE. UV crosslink, immunoblotting with HeLa nuclear extracts, EMSA with recombinant protein.
HuRAUUUAUUUAUUUAUUUA-8Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9155038Myer VE, Fan XC, Steitz JA. (1997)
Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.
EMBO J. 16(8):2130-2139.
HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. synthesized oligos EMSA, UV crosslink, competition assay with HeLa nuclear extracts.
HuRAUGUAUGUAUGUAUGUA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9155038Myer VE, Fan XC, Steitz JA. (1997)
Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.
EMBO J. 16(8):2130-2139.
HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. synthesized oligos EMSA, UV crosslink, competition assay with HeLa nuclear extracts.
HuRAUUUUAUUUUAUUUUA-3Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9155038Myer VE, Fan XC, Steitz JA. (1997)
Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.
EMBO J. 16(8):2130-2139.
HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. synthesized oligos EMSA, UV crosslink, competition assay with HeLa nuclear extracts.
HuRUUAUUUAUUGACCUUAUUUAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9155038Myer VE, Fan XC, Steitz JA. (1997)
Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay.
EMBO J. 16(8):2130-2139.
HuR has no affinity to (AUUU)2A, UUAUUUAUU, UAUUUAUGACCUAUUUAU, (AU3AGC)4A, (AGGU)4A, (CUUU)4C, (AU)8A, (AUU)5A. synthesized oligos EMSA, UV crosslink, competition assay with HeLa nuclear extracts.
HuRAUAUUUAUAUUUUUAUUUUAUUUUUUU-10Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
8626503Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996)
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
J Biol Chem. 271(14):8144-8151.
 WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562].  EMSA, Filter Binding Assay, RNase Protection Assay.
HuRAUACGUAUAUUUUUAUUUUAUUUUUUU-8Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
8626503Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996)
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
J Biol Chem. 271(14):8144-8151.
 WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562].  EMSA, Filter Binding Assay, RNase Protection Assay.
HuRAUAUUUAUAUCGCUAUUUUAUUUUUUU-3Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
8626503Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996)
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
J Biol Chem. 271(14):8144-8151.
 WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562].  EMSA, Filter Binding Assay, RNase Protection Assay.
HuRAUAUUUAUAUUUUUAUGCUAUUUUUUU-3Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
8626503Ma WJ, Cheng S, Campbell C, Wright A, Furneaux H. (1996)
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
J Biol Chem. 271(14):8144-8151.
 WT and mutants A/U rich elements (ARE) of 3'UTR of c-fos [2353], c-myc [4609], n-myc [4613], IL-3 [3562].  EMSA, Filter Binding Assay, RNase Protection Assay.
HuRUUUUUUU-7Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
9882309Sokolowski M, Furneaux H, Schwartz S. (1999)
The inhibitory activity of the AU-rich RNA element in the human papillomavirus type 1 late 3' untranslated region correlates with its affinity for the elav-like HuR protein.
J Virol. 73(2):1080-1091.
HuR has no affinity to poly(A), poly(G) and poly(C). Sequence and mutants of human Papillomavirus Type 1 Late 3' UTR h1ARE. UV crosslink, immunoblotting with HeLa nuclear extracts, EMSA with recombinant protein.
HuRUAUUAAUUUAAUUAUUUAAUAAUAUUUAUAUUAAA-1Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRUAUUUAUUUAUUUAUUUGUUUGUUUGUUUUAUU-10Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRUAUUUAUUUAAAUAUUUAAAUUUUAUAUUUAUU-2Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRAUAUUUUAAUUUAUGAGUUUUUGAUAGCUUUAUUUUUUAAGUAUUUAUAUAUUUAUAA-7Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRUAUUUAUUAUUUAUGUAUUUAUUUAA-9Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUA-10Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRUGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUACAGA-9Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRUGUGAUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUACAGA-7Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRAUUAUUUAUUAUUUAUUUAUUAAAUAUUUAUUUA-4Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRAUUUAUUUAUUUA-9Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRAUUUAUUUAUUUAUUUAUUUA-10Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRCUUUCUUUCUUUCUUUC-9Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15457527Meisner NC, Hackermuller J, Uhl V, Aszodi A, Jaritz M, Auer M. (2004)
mRNA openers and closers: modulating AU-rich element-controlled mRNA stability by a molecular switch in mRNA secondary structure.
Chembiochem. 5(10):1432-1447.
 Synthesized sequences. 2D-FIDA anisotropy with purified protein
HuRUUUGUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
HuRUUAUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
HuRUUUAUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
HuRUUGUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
HuRUUAUUUAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
10075998Maurer F, Tierney M, Medcalf RL. (1999)
An AU-rich sequence in the 3'-UTR of plasminogen activator inhibitor type 2 (PAI-2) mRNA promotes PAI-2 mRNA decay and provides a binding site for nuclear HuR.
Nucleic Acids Res. 27(7):1664-1673.
 Sequences deriving from SERPINB2 [5055] EMSA supershift, mutation analysis with HT-1080 NE
HuRUGUUUUUUUGAGAGU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
10913178Millard SS, Vidal A, Markus M, Koff A. (2000)
A U-rich element in the 5' untranslated region is necessary for the translation of p27 mRNA.
Mol Cell Biol. 20(16):5947-5959.
 Sequences deriving from CDKN1B [1027] UV cross-linking, immunoprecipitation, mutation analysis in 293T cell
HuRUAUUUCUUGUUUGUUUGUUUGGGUAU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11602610Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001)
HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities.
J Biol Chem. 276(51):47958-47965.
 Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] UV cross-linking, immunoprecipitation in HeLa NE
HuRUUUUUUUUUUUUUUUUUUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11602610Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001)
HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities.
J Biol Chem. 276(51):47958-47965.
 Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] UV cross-linking, immunoprecipitation in HeLa NE
HuRUAUUUAUAUUUUUAUUUUAUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11602610Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001)
HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities.
J Biol Chem. 276(51):47958-47965.
 Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] UV cross-linking, immunoprecipitation in HeLa NE
HuRUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11602610Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001)
HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities.
J Biol Chem. 276(51):47958-47965.
 Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] UV cross-linking, immunoprecipitation in HeLa NE
HuRUAUUUAUUUAUGUAUGUAUGUAUUUAUUUAUUUAU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11602610Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001)
HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities.
J Biol Chem. 276(51):47958-47965.
 Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] UV cross-linking, immunoprecipitation in HeLa NE
HuRUUACCAUCUUUUUUUUUUCUUUA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11602610Raghavan A, Robison RL, McNabb J, Miller CR, Williams DA, Bohjanen PR. (2001)
HuA and tristetraprolin are induced following T cell activation and display distinct but overlapping RNA binding specificities.
J Biol Chem. 276(51):47958-47965.
 Sequences deriving from 3' UTR of JUN [3725], FOS [2353], TNF [7124], CSF2 [1437], IL3 [3562], MYC [4609] UV cross-linking, immunoprecipitation in HeLa NE
HuRUUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
11719186Chen CY, Gherzi R, Ong SE, Chan EL, Raijmakers R, Pruijn GJ, Stoecklin G, Moroni C, Mann M, Karin M. (2001)
AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.
Cell. 107(4):451-464.
 Sequences deriving from FOS [2353] UV crosslinking, imunoprecipitation with Jurkat extracts
HuRCUUUCUCUCCUUUCUUUUUCUUCUUC-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
12011088Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002)
Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.
J Biol Chem. 277(30):27183-27192.
 Sequences deriving from AR [367]. Synthesized oligos. Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts
HuRCCCCCCC-7Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
12011088Yeap BB, Voon DC, Vivian JP, McCulloch RK, Thomson AM, Giles KM, Czyzyk-Krzeska MF, Furneaux H, Wilce MC, Wilce JA, Leedman PJ. (2002)
Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.
J Biol Chem. 277(30):27183-27192.
 Sequences deriving from AR [367]. Synthesized oligos. Mutational analysis, UV cross-linking, Immunoprecipitation, competition assay in LNCaP extracts
HuRCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUCUUUUUUUUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
12605686Wein G, Rossler M, Klug R, Herget T. (2003)
The 3'-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing factors HuD and HuR.
Eur J Biochem. 270(2):350-365.
 Sequences deriving from mouse Marcks [17118] EMSA, UV cross-linking with recombinant protein
HuRUCUAUUAAUUUAAUUAUUUAAUAAUAUUUAUAUUAAACUCCUUAU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
12704185Sengupta S, Jang BC, Wu MT, Paik JH, Furneaux H, Hla T. (2003)
The RNA-binding protein HuR regulates the expression of cyclooxygenase-2.
J Biol Chem. 278(27):25227-25233.
 Sequences deriving from PTGS2 [5743] RNase T1 assay with recombinant protein
HuRCUUAAAUUCAUUUCACACAUUAAUUUUAUCUCA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
12704185Sengupta S, Jang BC, Wu MT, Paik JH, Furneaux H, Hla T. (2003)
The RNA-binding protein HuR regulates the expression of cyclooxygenase-2.
J Biol Chem. 278(27):25227-25233.
 Sequences deriving from PTGS2 [5743] RNase T1 assay with recombinant protein
HuRUGCAUGCUGUUCCUUUUCUUUUCUUCUUUUAGCCAUUUUGC-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
12704185Sengupta S, Jang BC, Wu MT, Paik JH, Furneaux H, Hla T. (2003)
The RNA-binding protein HuR regulates the expression of cyclooxygenase-2.
J Biol Chem. 278(27):25227-25233.
 Sequences deriving from PTGS2 [5743] RNase T1 assay with recombinant protein
HuRGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUAG-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
15809297Fialcowitz EJ, Brewer BY, Keenan BP, Wilson GM. (2005)
A hairpin-like structure within an AU-rich mRNA-destabilizing element regulates trans-factor binding selectivity and mRNA decay kinetics.
J Biol Chem. 280(23):22406-22417.
 Synthesized sequences EMSA, fluorescence anisotropy with recombinant protein
HuRUAUUUAUGUUUGCACUUGUGAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUACAGAUGAAUGUAUUUAUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16168373Katsanou V, Papadaki O, Milatos S, Blackshear PJ, Anderson P, Kollias G, Kontoyiannis DL. (2005)
HuR as a negative posttranscriptional modulator in inflammation.
Mol Cell. 19(6):777-789.
 Sequences deriving from wt and mutant TNF [7194] EMSA with recombinant protein
HuRUUAUUUAUAAAUCAUUUCCUUUCUUUUUUUCCCCAAAGUCAGAAUUGCUCAAAGAAAAUUAUUUAUUGUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16289864Sommer S, Cui Y, Brewer G, Fuqua SA. (2005)
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
J Steroid Biochem Mol Biol. 97(3):219-229.
 Sequences deriving from YES1 [7525] EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts.
HuRUUUUUUAAAGUUUCUUGCAUUUAUUAUUCUCAAAAGUUUUUUCUAAGUUAAACAGUCAGUAUGCAAUCUUAAUAUAUGCUUUCUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16289864Sommer S, Cui Y, Brewer G, Fuqua SA. (2005)
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
J Steroid Biochem Mol Biol. 97(3):219-229.
 Sequences deriving from YES1 [7525] EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts.
HuRUUUUUAUGUAAAACAUUUUUAGAACUCCAGUUUUCAAAUCAUGUUUGAAUCUACAUUCACUUUUUUUUGUUUUCUUUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16289864Sommer S, Cui Y, Brewer G, Fuqua SA. (2005)
The c-Yes 3'-UTR contains adenine/uridine-rich elements that bind AUF1 and HuR involved in mRNA decay in breast cancer cells.
J Steroid Biochem Mol Biol. 97(3):219-229.
 Sequences deriving from YES1 [7525] EMSA supershift with MDA-MB-231 protein extract and recombinant protein. UV-cross link, immunoprecipitation with MDA-MB-231 protein extracts.
HuRUGAACUUUAAAGUUAUAGUUGUUUUAUAUGUU-2Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
HuRUGAACUUUAUAGUUGUUUUAUAUGUU-2Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16787927Pullmann R Jr, Abdelmohsen K, Lal A, Martindale JL, Ladner RD, Gorospe M. (2006)
Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1.
J Biol Chem. 281(33):23456-23463.
 Sequences deriving from wt and mutated TYMS [7298] Pulldown assay and Western blotting in RKO whole-cell extract.
HuRUAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
16890199Izquierdo JM. (2006)
Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR.
Biochem Biophys Res Commun. 348(2):703-711.
 Sequences deriving from ATP5B [506] UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein.
HuRUUUUGUUUUGGUUUUUUUUUUUUUUUUGGC-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
17548472Dormoy-Raclet V, Menard I, Clair E, Kurban G, Mazroui R, Di Marco S, von Roretz C, Pause A, Gallouzi IE. (2007)
The RNA-binding protein HuR promotes cell migration and cell invasion by stabilizing the beta-actin mRNA in a U-rich-element-dependent manner.
Mol Cell Biol. 27(15):5365-5380.
 Sequences deriving from ACTB [60] EMSA supershift with HeLa cell extracts
HuRGGGGGGUUUUUUUUUUUUUUUUUGGGGG-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
17682065Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA. (2007)
Elucidation of a C-rich signature motif in target mRNAs of RNA-binding protein TIAR.
Mol Cell Biol. 27(19):6806-6817.
 Synthesized sequences Surface plasmon resonance
HuRUUUGUCUUCUUCUUUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
18463097Izquierdo JM. (2008)
Hu antigen R (HuR) functions as an alternative pre-mRNA splicing regulator of Fas apoptosis-promoting receptor on exon definition.
J Biol Chem. 283(27):19077-19084.
 Sequences deriving from FAS [355]. Constructs of FAS [355] EX5 - INT5 - EX6 - INT6 - EX7.In vivo splicing in HeLa.UV cross-linking, immunoprecipitation with HeLa NE. siRNA.
HuRCUUUUCUGUUUAGUUUUUACUUUUUUUGUUUUGUUUUUUUA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
18644987Scott GK, Marx C, Berger CE, Saunders LR, Verdin E, Schafer S, Jung M, Benz CC. (2008)
Destabilization of ERBB2 transcripts by targeting 3' untranslated region messenger RNA associated HuR and histone deacetylase-6.
Mol Cancer Res. 6(7):1250-1258.
 Sequences deriving from ERBB2 [2064] EMSA supershift with SKBR3 cytosol extract.
HuRCAUAAAUUAUUUUCAAGUGUAACUUAUUAACCUAUUUAUUAUUUAUGUAUUUAUUUAAGC-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
18650551Palanisamy V, Park NJ, Wang J, Wong DT. (2008)
AUF1 and HuR proteins stabilize interleukin-8 mRNA in human saliva.
J Dent Res. 87(8):772-776.
 Sequences deriving from IL8 [3576] UV cross-linking, immunoprecipitation with salivary protein extracts.
HuRGUGUUGUUUGUUGUGUAUAUGUUUGUAUGU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
18986664Cumming SA, Chuen-Im T, Zhang J, Graham SV. (2009)
The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells.
Virology. 383(1):142-149.
 Sequences deriving from HPV-16 LRE. EMSA supershift, UV cross-linking with W12 NE or recombinant protein
HuRGAUGCUGAUUCAUUAUUUAUCAGCCCUAUUCUUUC-8Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19010922Saunus JM, French JD, Edwards SL, Beveridge DJ, Hatchell EC, Wagner SA, Stein SR, Davidson A, Simpson KJ, Francis GD, Leedman PJ, Brown MA. (2008)
Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR.
Cancer Res. 68(22):9469-9478.
 Sequences deriving from BRCA1 [672] EMSA supershift, UV cross-linking with HeLa protein extracts
HuRGAAGCUGAUUCAUUAUUUAUCAGCCCUAUUCUUUC-8Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19010922Saunus JM, French JD, Edwards SL, Beveridge DJ, Hatchell EC, Wagner SA, Stein SR, Davidson A, Simpson KJ, Francis GD, Leedman PJ, Brown MA. (2008)
Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR.
Cancer Res. 68(22):9469-9478.
 Sequences deriving from BRCA1 [672] EMSA supershift, UV cross-linking with HeLa protein extracts
HuRGAUGCUGAGUCAUUAUUUAUCAGCCCUAUUCUUUC-2Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19010922Saunus JM, French JD, Edwards SL, Beveridge DJ, Hatchell EC, Wagner SA, Stein SR, Davidson A, Simpson KJ, Francis GD, Leedman PJ, Brown MA. (2008)
Posttranscriptional regulation of the breast cancer susceptibility gene BRCA1 by the RNA binding protein HuR.
Cancer Res. 68(22):9469-9478.
 Sequences deriving from BRCA1 [672] EMSA supershift, UV cross-linking with HeLa protein extracts
HuRCUAGUAGAACCUUCUUUCCUAAUCCCCUUAUCUUCAUGGAAAUGGACUGACUUUAUGCCUAUGAAGUCC-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19151756Woo HH, Zhou Y, Yi X, David CL, Zheng W, Gilmore-Hebert M, Kluger HM, Ulukus EC, Baker T, Stoffer JB, Chambers SK. (2009)
Regulation of non-AU-rich element containing c-fms proto-oncogene expression by HuR in breast cancer.
Oncogene. 28(9):1176-1186.
 Sequences deriving from CSF1R [1436] UV cross-linking with recombinant protein
HuRCUGUAUUUAUCUGUUUAUUUAUACCUAUUUAUGUAUUUAUUUAUUGAAGUGUGAAAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
19359363Al-Ahmadi W, Al-Ghamdi M, Al-Haj L, Al-Saif M, Khabar KS. (2009)
Alternative polyadenylation variants of the RNA binding protein, HuR: abundance, role of AU-rich elements and auto-Regulation.
Nucleic Acids Res. 37(11):3612-3624.
 Sequences deriving from ELAVL1 [1994] EMSA supershift with HEK293 protein lysate or recombinant protein.
HuRGCAUGCUGUUCCUUUUCUUUUCU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
20086103Doller A, Schlepckow K, Schwalbe H, Pfeilschifter J, Eberhardt W. (2010)
Tandem phosphorylation of serines 221 and 318 by protein kinase Cdelta coordinates mRNA binding and nucleocytoplasmic shuttling of HuR.
Mol Cell Biol. 30(6):1397-1410.
 Sequences deriving from PTGS2 [5743] EMSA with recombinant protein.
HuRUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUUCUACCUUGAGGUCUUUUGA-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
20459669Ahn J, Byeon IJ, Dharmasena S, Huber K, Concel J, Gronenborn AM, Sluis-Cremer N. (2010)
The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro.
Retrovirology. 7:40.
 Synthesized sequences EMSA with recombinant protein
HuRCCAUUUAUAUCAUUUUUUAUAUAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
HuRCCAUUUAUAUCAUAAAAUAUAUAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
HuRCCAUUUAUAUCAUUUUUAAAAAAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
HuRCCAUUUAUAUCAUAAAAAUAUUAUU-5Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
20498276Chang N, Yi J, Guo G, Liu X, Shang Y, Tong T, Cui Q, Zhan M, Gorospe M, Wang W. (2010)
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Mol Cell Biol. 30(15):3875-3886.
 Sequences deriving from CDKN2A [1029] Protein affinity purification, western blotting with HeLa cytoplasmic extracts
HuRGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
HuRAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: ELAVL1, ELAV (embryonic lethal abnormal vision Drosophila)-like 1 (Hu antigen R), Hua, MelG, ELAV1
HuR carries bidirectional shuttling signals that serve for both nuclear localization and export (PMID:9860962).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
KSRPUGCAUG-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
KSRPUUAGGGUCACACCCACCACUGGGAGAU-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 19458619Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009)
The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs.
Nature. 459(7249):1010-1014.
 MIRLET7A1 [406881], MIR21 [406991] and mutants. EMSA with purified protein
KSRPUUAGGGUCACACCCACCACUCCCAGAU-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 19458619Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009)
The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs.
Nature. 459(7249):1010-1014.
 MIRLET7A1 [406881], MIR21 [406991] and mutants. EMSA with purified protein
KSRPCUGUUGAAUCUCAUGG-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 19458619Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009)
The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs.
Nature. 459(7249):1010-1014.
 MIRLET7A1 [406881], MIR21 [406991] and mutants. EMSA with purified protein
KSRPCUGUUGAAUCUCAUCC-2Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 19458619Trabucchi M, Briata P, Garcia-Mayoral M, Haase AD, Filipowicz W, Ramos A, Gherzi R, Rosenfeld MG. (2009)
The RNA-binding protein KSRP promotes the biogenesis of a subset of microRNAs.
Nature. 459(7249):1010-1014.
 MIRLET7A1 [406881], MIR21 [406991] and mutants. EMSA with purified protein
KSRPUUUUAUUGUGUUUUUAAUUUAUUUAUUAAGAUGGAUUCUCAGAUAUUUAUAUUUUUAUUUUAUUUUUUU-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 11719186Chen CY, Gherzi R, Ong SE, Chan EL, Raijmakers R, Pruijn GJ, Stoecklin G, Moroni C, Mann M, Karin M. (2001)
AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.
Cell. 107(4):451-464.
 Sequences deriving from FOS [2353] UV crosslinking, imunoprecipitation with Jurkat extracts
KSRPAUUUUA-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 16126846Linker K, Pautz A, Fechir M, Hubrich T, Greeve J, Kleinert H. (2005)
Involvement of KSRP in the post-transcriptional regulation of human iNOS expression-complex interplay of KSRP with TTP and HuR.
Nucleic Acids Res. 33(15):4813-4827.
 Sequences deriving from NOS2 [4843] UV cross-linking with recombinant protein
KSRPCAUAAAUUAUUUUCAAGUGUAACUUAUUAACCUAUUUAUUAUUUAUGUAUUUAUUUAAGC-10Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17908789Winzen R, Thakur BK, Dittrich-Breiholz O, Shah M, Redich N, Dhamija S, Kracht M, Holtmann H. (2007)
Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets.
Mol Cell Biol. 27(23):8388-8400.
 Sequences deriving from IL8 [3576] EMSA with recombinant protein
KSRPCUAUUUAUUAUUUAUGUAUGUAUUUAAGC-9Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17908789Winzen R, Thakur BK, Dittrich-Breiholz O, Shah M, Redich N, Dhamija S, Kracht M, Holtmann H. (2007)
Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets.
Mol Cell Biol. 27(23):8388-8400.
 Sequences deriving from IL8 [3576] EMSA with recombinant protein
KSRPCUAUUUAUUAUUUAUGUAUUUAUUUAAGC-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17908789Winzen R, Thakur BK, Dittrich-Breiholz O, Shah M, Redich N, Dhamija S, Kracht M, Holtmann H. (2007)
Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets.
Mol Cell Biol. 27(23):8388-8400.
 Sequences deriving from IL8 [3576] EMSA with recombinant protein
KSRPUAUUUAUUAUUUAUUUAUUAUUUAU-10Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17437720Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007)
The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation.
Structure. 15(4):485-498.
 Sequences deriving from TNF [7124] NMR and CD with recombinant protein
KSRPUAUUUAUUAUUU-10Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17437720Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007)
The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation.
Structure. 15(4):485-498.
 Sequences deriving from TNF [7124] NMR and CD with recombinant protein
KSRPUAUUUA-2Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17437720Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007)
The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation.
Structure. 15(4):485-498.
 Sequences deriving from TNF [7124] NMR and CD with recombinant protein
KSRPUAUUAU-2Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 17437720Garcia-Mayoral MF, Hollingworth D, Masino L, Diaz-Moreno I, Kelly G, Gherzi R, Chou CF, Chen CY, Ramos A. (2007)
The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation.
Structure. 15(4):485-498.
 Sequences deriving from TNF [7124] NMR and CD with recombinant protein
KSRPGUCUCUUCCAAUGAUUCCAUUUCAAUAUAUUCUUCUUUUUAAAGUAUUACACAUUUCCACUU-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18583400Nechama M, Ben-Dov IZ, Briata P, Gherzi R, Naveh-Many T. (2008)
The mRNA decay promoting factor K-homology splicing regulator protein post-transcriptionally determines parathyroid hormone mRNA levels.
FASEB J. 22(10):3458-3468.
 Sequences deriving from rat Pth [24694] UV cross-linking, competition assay with HEK293 total extracts.
KSRPUUGGG-2Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18684992Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008)
The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets.
Nucleic Acids Res. 36(16):5290-5296.
 Synthesized oligos NMR, scaffold-independent analysis (SIA) with recombinant protein
KSRPUUUAG-1Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18684992Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008)
The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets.
Nucleic Acids Res. 36(16):5290-5296.
 Synthesized oligos NMR, scaffold-independent analysis (SIA) with recombinant protein
KSRPUGGGU-10Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18684992Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008)
The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets.
Nucleic Acids Res. 36(16):5290-5296.
 Synthesized oligos NMR, scaffold-independent analysis (SIA) with recombinant protein
KSRPUAGGG-5Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18684992Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008)
The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets.
Nucleic Acids Res. 36(16):5290-5296.
 Synthesized oligos NMR, scaffold-independent analysis (SIA) with recombinant protein
KSRPUAGUAU-6Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18684992Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008)
The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets.
Nucleic Acids Res. 36(16):5290-5296.
 Synthesized oligos NMR, scaffold-independent analysis (SIA) with recombinant protein
KSRPUAGGUA-7Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 18684992Garcia-Mayoral MF, Diaz-Moreno I, Hollingworth D, Ramos A. (2008)
The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets.
Nucleic Acids Res. 36(16):5290-5296.
 Synthesized oligos NMR, scaffold-independent analysis (SIA) with recombinant protein
KSRPGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-6Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
KSRPAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-4Gene Name and Synonymous: KHSRP, KH-type splicing regulatory protein, FBP2, FUBP2, MGC99676. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
MBNL1UGUCUCGCU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
15257297Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004)
Muscleblind proteins regulate alternative splicing.
EMBO J. 23(15): 3103-3112.
MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. Mutational analysis, UV-crosslinking assay using purified recombinant protein.
MBNL1CGCUGCGGC-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
15257297Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004)
Muscleblind proteins regulate alternative splicing.
EMBO J. 23(15): 3103-3112.
MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. Mutational analysis, UV-crosslinking assay using purified recombinant protein.
MBNL1CGCUUU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
15257297Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004)
Muscleblind proteins regulate alternative splicing.
EMBO J. 23(15): 3103-3112.
MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. Mutational analysis, UV-crosslinking assay using purified recombinant protein.
MBNL1UGCUGCUUUU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
15257297Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. (2004)
Muscleblind proteins regulate alternative splicing.
EMBO J. 23(15): 3103-3112.
MBNL1 promotes exon skipping of human and chicken cTNT EX5 and inclusion of IR EX11 in primary chicken skeletal muscle cultures. WT and mutated RNA substrates of the human cTNT [TNNT2, 7139] and chicken cTNT [TNNT2, 396433] gene. Mutational analysis, UV-crosslinking assay using purified recombinant protein.
MBNL1CUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUG-6Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUG-8Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCG-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAGCCAG-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAGCAAG-6Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUGCUUG-6Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCGCCCG-8Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CAUGCAUGCAUGCAUGCAUGCAUGCAUG-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CCUGCCUGCCUGUCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CCUGCCUGCCUGCCUGCCUGGCUGCCUGUCUGCCUGCCUGCCUGCCUG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CUGCUGUUCGCUGCUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CUGCUGCUGUUCGCUGCUGCUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CUGCUGCUGCUGUUCGCUGCUGCUGCUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CCUGCCUGUUCGCCUGCCUG-8Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CCUGCCUGCCUGUUCGCCUGCCUGCCUG-9Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CUGCCUGCCUGUUCGCUGCCUGCCUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CCUGCCUGCCUGCCUGUUCGCCUGCCUGCCUGCCUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CCGCCGUUCGCCGCCG-9Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CUGCUGUUCGCCGCCG-9Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CCGCCGUUCGCAGCAG-7Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CAGCAGUUCGCAGCAG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CAGCAGUUCGCGGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CGGCGGUUCGCGGCGG-3Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CGGCGGUUCGCUGCUG-2Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1GUCUCGCGUUCGCGCUGCGGC-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1GUCUCGCUGUUCGCGCUGCGGC-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CUGUCUCGUUCGCGCUGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1UGUCUCGCUUUUCCCCUCCGCUGCGGC-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1UGUCUGCUGUUUUCCCCUCCCUGCUGGC-2Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1UUUCUCCCUUUUCCCCUCCCCUUCGGC-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1UGUCUCUCUUUUCCCCUCCGCGGCGGC-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1GCGCUUGUGC-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17702765Yuan Y, Compton SA, Sobczak K, Stenberg MG, Thornton CA, Griffith JD, Swanson MS. (2007)
Muscleblind-like 1 interacts with RNA hairpins in splicing target and pathogenic RNAs.
Nucleic Acids Res. 35(16): 5474-5486.
 WT and mutated RNA substrates of TNNT3 [7140] INT8. UV-crosslinking, mutational analysis, using HEK293T lysate overexpressing recombinant protein.
MBNL1CCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAGCCUAG-2Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
14722159Kino Y, Mori D, Oma Y, Takeshita Y, Sasagawa N, Ishiura S. (2004)
Muscleblind protein, MBNL1/EXP, binds specifically to CHHG repeats.
Hum. Mol. Genet. 13:495–507.
 Synthesized sequences. Yeast three-hybrid assay confirmed by EMSA using purified recombinant protein. EMSA with competing probes.
MBNL1CUCCUCUUCGGUGGUG-2Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
17942744Warf MB, Berglund JA. (2007)
MBNL binds similar RNA structures in the CUG repeats of myotonic dystrophy and its pre-mRNA substrate cardiac troponin T.
RNA. 13(12): 2238-2251.
 Synthesized sequences. WT and mutated RNA substrates of cTNT [TNNT2, 7139] gene. Construct of TNNT2 [7139] EX4 - INT4 - EX5.In vivo splicing in HeLa cells.EMSA using recombinant protein.
MBNL1CUGCUU-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UCGCUU-8Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCUU-7Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCUG-4Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCUA-3Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1ACGCUU-3Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCGCUG-3Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCCU-3Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCGCUU-3Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1AUGCUU-2Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCUUCUGCUUCUGCUUCUGCUUCUGCUUCUGCUU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCCUCUGCCUCUGCCUCUGCCUCUGCCUCUGCCU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCGCUUCCGCUUCCGCUUCCGCUUCCGCUUCCGCUU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCUCUGCUUGCUUGCUUGCUGUUUAUGUUAAUGCGCUCGCUUGAACCCCACUGGCCC-9Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCUUUAUUGUGCAUGCUUGCUUAGUCUUGUUAUUCGUUGUAUAU-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UGCCACUGCUGCUGCUUGCUGCUGCUGCGCUCGCUUCCAGUCAGGGUGGGCCGC-8Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GCACCUCUGCUUGCUUGCUUGCUGUUUACCUGUAUGUUAAUUCGCUCGCUUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GCCGAGGAGGUGGUGUGAUUGCUUGCUUUAGCGCCGUCAUUUUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GCGGCGGGCAGCUGUGCUUGCUGGAGAGCAGAUGCUUGCUUCACCAAU-2Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GGCUUU5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20519504Sen S, Talukdar I, Liu Y, Tam J, Reddy S, Webster NJ. (2010)
Muscleblind-like 1 (Mbnl1) promotes insulin receptor exon 11 inclusion via binding to a downstream evolutionarily conserved intronic enhancer.
J Biol Chem. 285(33):25426-25437.
 Sequences deriving from INSR [3643] and rat Insr [24954]. Constructs of INSR [3643] EX10 - INT10 - EX11 - INT11 - EX12.In vivo splicing in HeLa, HepG2 and HEK293RNA-affinity purification, western blot with HeLa NE
MBNL1GGGAUUUAUGGGGCUUUUUA5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20519504Sen S, Talukdar I, Liu Y, Tam J, Reddy S, Webster NJ. (2010)
Muscleblind-like 1 (Mbnl1) promotes insulin receptor exon 11 inclusion via binding to a downstream evolutionarily conserved intronic enhancer.
J Biol Chem. 285(33):25426-25437.
 Sequences deriving from INSR [3643] and rat Insr [24954]. Constructs of INSR [3643] EX10 - INT10 - EX11 - INT11 - EX12.In vivo splicing in HeLa, HepG2 and HEK293RNA-affinity purification, western blot with HeLa NE
MBNL1UUGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCUG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCGCUA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UCGCUG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCUA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CCGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UCGCUA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1AUGCCG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCCG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCCU-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCCA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCCC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1AUGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GUGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1ACGCUA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1ACGCUG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GCGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GCGCUG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GCGCUU-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCCC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UUGCCG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GUGCUA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GUGCUG-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1GUGCUU-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1ACGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1UCGCUC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1AUGCCA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1AUGCCC-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CUGCCA-1Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20071745Goers ES, Purcell J, Voelker RB, Gates DP, Berglund JA. (2010)
MBNL1 binds GC motifs embedded in pyrimidines to regulate alternative splicing.
Nucleic Acids Res. 38(7):2467-2484.
 Sequences of 32nt random for SELEX. Synthesized sequences. Sequence deriving from mutated MBNL1 [4154], MBNL2 [10150], mutated ATP2A1 [487], GRIN1 [2902], INSR [3643]. Construct of PLEKHH2 [130271] EX20 - INT20 - EX21 - INT21 - EX22.In vivo splicing in HeLaSELEX of 32nt random and EMSA with recombinant protein.
MBNL1CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
20186122Sellier C, Rau F, Liu Y, Tassone F, Hukema RK, Gattoni R, Schneider A, Richard S, Willemsen R, Elliott DJ, Hagerman PJ, Charlet-Berguerand N. (2010)
Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients.
EMBO J. 29(7):1248-1261.
 Sequences deriving from FMR1 [2332]. FISH/IF and immunohistochemistry in human brain (hippocampal sections) of FXTAS patients.
MBNL1CCCGCUGCUGCUGCUGCUGCUGCUGCUGCUGCUGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
24377303Yadav AR, Mace CR, Miller BL. (2014)
Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method.
Anal Chem. 86(2):1067-1075.
 Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein.
MBNL1CCCGCUGCUGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
24377303Yadav AR, Mace CR, Miller BL. (2014)
Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method.
Anal Chem. 86(2):1067-1075.
 Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein.
MBNL1CCCGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCCUGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
24377303Yadav AR, Mace CR, Miller BL. (2014)
Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method.
Anal Chem. 86(2):1067-1075.
 Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein.
MBNL1CCCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
24377303Yadav AR, Mace CR, Miller BL. (2014)
Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method.
Anal Chem. 86(2):1067-1075.
 Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein.
MBNL1CCCGCUGCUGCUGCUGCUGCAGCAGCAGCAGCAGCGG-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
24377303Yadav AR, Mace CR, Miller BL. (2014)
Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method.
Anal Chem. 86(2):1067-1075.
 Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein.
MBNL1AUAUAUAUAUGUGCGCUUGUGCCCACAUAUAUAUA-5Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
24377303Yadav AR, Mace CR, Miller BL. (2014)
Examining the interactions of the splicing factor MBNL1 with target RNA sequences via a label-free, multiplex method.
Anal Chem. 86(2):1067-1075.
 Synthetic sequences. Sequences deriving from mouse Tnnt3 [21957]. Aqueous arrayed imaging reflectometry on a microarrayed chip, SPR using recombinant protein.
MBNL1CUGCUGCUGCUGCUGCUG-10Gene Name and Synonymous: MBNL1, muscleblind-like (Drosophila), EXP, MBNL, EXP35, EXP40, EXP42, KIAA0428, DKFZp686P06174.
MBNL proteins can act as activators or repressors of splicing on different pre-mRNAs (PMID: 15257297).
MBNLs are dsRNA binding factors that can bind CUG or CCUG repeats (PMID: 14722159).
23423380Reddy K, Zamiri B, Stanley SY, Macgregor RB Jr, Pearson CE. (2013)
The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures.
J Biol Chem. 288(14):9860-9866.
 Synthetic sequences. EMSA with recombinant protein.
Nova-1AUCACC10Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1ACCACC6Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1UCAUAAGUCAUAAACAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUCACAUUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUCAUUCAUUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUCGCAUUUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUUAACAUUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUUCAUCGUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUUCAUUCAU7Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUUUCAUCUACAUAUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUUUCAUCUCAU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUUUCAUUUCAC5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCUGGCCAUGCAUCA5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1GGGGGGG7Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 8558240Buckanovich RJ, Yang YY, Darnell RB. (1996)
The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies.
J Neurosci. 16(3):1114-1122.
Nova-1 has no affinity to poly(A). Homopolymers Immunoblots and Filter Binding Assay.
Nova-1CCCCCCC3Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 8558240Buckanovich RJ, Yang YY, Darnell RB. (1996)
The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies.
J Neurosci. 16(3):1114-1122.
Nova-1 has no affinity to poly(A). Homopolymers Immunoblots and Filter Binding Assay.
Nova-1UUUUUUU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 8558240Buckanovich RJ, Yang YY, Darnell RB. (1996)
The onconeural antigen Nova-1 is a neuron-specific RNA-binding protein, the activity of which is inhibited by paraneoplastic antibodies.
J Neurosci. 16(3):1114-1122.
Nova-1 has no affinity to poly(A). Homopolymers Immunoblots and Filter Binding Assay.
Nova-1UCAUUUUCAUUUUCAUUU5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UCAUAAUCAUAAUCAUAA5Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 9154818Buckanovich RJ, Darnell RB. (1997)
The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo.
Mol Cell Biol. 17(6):3194-3201.
 Sequences of 52 nt random for SELEX. SELEX of 52nt random with recombinant protein. Winners and mutants confermed by Filter Binding Assays and EMSA with recombinant protein.
Nova-1UUCAU8Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 17655233Beuth B, Garcia-Mayoral MF, Taylor IA, Ramos A. (2007)
Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex.
J Am Chem Soc. 129(33):10205-10210.
 Synthesized oligos NMR titrations using recombinant protein
Nova-1UUCAG8Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 17655233Beuth B, Garcia-Mayoral MF, Taylor IA, Ramos A. (2007)
Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex.
J Am Chem Soc. 129(33):10205-10210.
 Synthesized oligos NMR titrations using recombinant protein
Nova-1UGUGU2Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 17655233Beuth B, Garcia-Mayoral MF, Taylor IA, Ramos A. (2007)
Scaffold-independent analysis of RNA-protein interactions: the Nova-1 KH3-RNA complex.
J Am Chem Soc. 129(33):10205-10210.
 Synthesized oligos NMR titrations using recombinant protein
Nova-1AUCAUC6Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1AGCACC3Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1AACACC3Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1AUCAAC3Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1UUCAAC6Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-1CUCAAC6Gene Name and Synonymous: NOVA1, neuro-oncological ventral antigen 1. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2AUCACC10Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2ACCACC6Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2GAGACAU1Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 9789075Yang YY, Yin GL, Darnell RB. (1998)
The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia.
Proc Natl Acad Sci U S A. 95(22):13254-13259.
Nova-2 has no affinity to poly(A) and poly(C). Homopolymers for immunoblot. Sequences 52-nt random for SELEX. SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers.
Nova-2GAGUCAU10Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 9789075Yang YY, Yin GL, Darnell RB. (1998)
The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia.
Proc Natl Acad Sci U S A. 95(22):13254-13259.
Nova-2 has no affinity to poly(A) and poly(C). Homopolymers for immunoblot. Sequences 52-nt random for SELEX. SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers.
Nova-2GAGGCAU1Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 9789075Yang YY, Yin GL, Darnell RB. (1998)
The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia.
Proc Natl Acad Sci U S A. 95(22):13254-13259.
Nova-2 has no affinity to poly(A) and poly(C). Homopolymers for immunoblot. Sequences 52-nt random for SELEX. SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers.
Nova-2UUUUUUU7Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 9789075Yang YY, Yin GL, Darnell RB. (1998)
The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia.
Proc Natl Acad Sci U S A. 95(22):13254-13259.
Nova-2 has no affinity to poly(A) and poly(C). Homopolymers for immunoblot. Sequences 52-nt random for SELEX. SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers.
Nova-2GGGGGGG7Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 9789075Yang YY, Yin GL, Darnell RB. (1998)
The neuronal RNA-binding protein Nova-2 is implicated as the autoantigen targeted in POMA patients with dementia.
Proc Natl Acad Sci U S A. 95(22):13254-13259.
Nova-2 has no affinity to poly(A) and poly(C). Homopolymers for immunoblot. Sequences 52-nt random for SELEX. SELEX of 52-nt random with protein, Filter Binding Assays. Immunoblot of homopolymers.
Nova-2AUCAC5Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10676814Lewis HA, Musunuru K, Jensen KB, Edo C, Chen H, Darnell RB, Burley SK. (2000)
Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome.
Cell. 100(3):323-332.
 Synthesized sequences X-ray crystallography with recombinant protein.
Nova-2AUCAUC6Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2AGCACC3Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2AACACC3Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2AUCAAC3Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2UUCAAC6Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
Nova-2CUCAAC6Gene Name and Synonymous: NOVA2, neuro-oncological ventral antigen 2, ANOVA, NOVA3. 10811881Jensen KB, Musunuru K, Lewis HA, Burley SK, Darnell RB. (2000)
The tetranucleotide UCAY directs the specific recognition of RNA by the nova K-homology 3 domain.
Proc Natl Acad Sci U S A. 97(11): 5740-5745.
 Sequences of 25-nt random. SELEX of 25nt random with recombinant protein. EMSA and Filter Binding Assay with recombinant protein.
nPTBCUCUCU-5Gene Name and Synonymous: PTBP2, polypyrimidine tract binding protein 2, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897.
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11003644Markovtsov V, Nikolic JM, Goldman JA, Turck CW, Chou MY, Black DL. (2000)
Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.
Mol Cell Biol. 20(20):7463-7479.
 Sequences from position 37 to 70 downstream murine c-src [20779] EX_N1 for EMSA and UV crosslink. Synthesized 37-mer RNA for RNA affinity purification. Construct of EX3 - INT - EX4 murine c-src for in vitro splicing.In vitro splicing with WERI-1 extracts.RNA affinity purification with HeLa or WERI-1 nuclear extracts. UV crosslink in HeLa and in WERI-1 extracts. EMSA with purified protein. Immunoprecipitation and Western blot.
nPTBCUGAGGCUGCCCGCUGCUCUCUGCAUGUGCUUCCU-5Gene Name and Synonymous: PTBP2, polypyrimidine tract binding protein 2, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897.
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
11571276Caputi M, Zahler AM. (2001)
Determination of the RNA Binding Specificity of the Heterogeneous Nuclear Ribonucleoprotein (hnRNP) H/H'/F/2H9 Family.
J Biol Chem. 276(47): 43850-43859.
 Synthesized oligos, constructs of Rev-dependent export (p17gag INS), beta-tropomyosin TPM2 [7169], c-src [20779]. RNA affinity binding assays, immunoblot with WT HeLa nuclear extracts or depleted by RNA affinity chromatography.
nPTBUUUAGUCAGCCUUAUAGCUAA-5Gene Name and Synonymous: PTBP2, polypyrimidine tract binding protein 2, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897.
nPTB functionally compensates for PTB and is upregulated when PTB is removed (PMID:17679092).
22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
PSFUUUUUUU-7Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 8449401Patton JG, Porro EB, Galceran J, Tempst P, Nadal-Ginard B. (1993)
Cloning and characterization of PSF, a novel pre-mRNA splicing factor.
Genes Dev. 7(3):393-406.
PSF has no affinity to poly(rA), poly(rC) and poly(rG). Construct of tropomyosin 1 alpha TPM1 [7168] EX2 - INT2 - EX3 for splicing. Construct of branchpoint and polypyrimidine tract element upstream of TPM1 EX3 for UV-crosslink.In vitro splicing in HeLa nuclear extracts.RNA affinity chromatography confirmed by UV crosslink and Western blot using HeLa nuclear extracts.
PSFCACUGCAUUCUCACCCGCAAGCA-5Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
PSFCACUGGAUUCUCACCCGGAAGUA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17664280Melton AA, Jackson J, Wang J, Lynch KW. (2007)
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Mol Cell Biol. 27(19): 6972-6984.
In this context, hnRNP K, hnRNP D, hnRNP A1, hnRNP A2/B1 do no bind CACUGGAUUCUCACCGGAAGUA and hnRNP A1, hnRNP A2/B1 do no bind CACUGCAUUCUCACCGCAAGCA. Synthesized sequences. Construct CD45 [PTPRC, 5788] EX3 - INT - EX4 - INT - EX7.In vitro splicing in JSL1 nuclear extract.Western blotting analysis of direct RNA affinity purifications of JSL1 nuclear extract.
PSFAAGGACC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFAGAGGAA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFAGAGGUA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGAAGAGGAA-6Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGAGAGGA-10Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGGACUGGGA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGGAGGAAC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGGAGGG-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGGGGGGGAUC-6Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGGUAAGAGC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUAAGGGAUC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUAGAGAGG-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUAGAUCGGAA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUAGGGGGAC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUCUAAGGAA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUGCAGGCA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUGGAAGAAC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUGGAGGAC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUGGCAGGGGGAUC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUGGUCUGGAGC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUUGAAGCAA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFUUGUUUGAUUUCUUAAAGU-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17507659Hall-Pogar T, Liang S, Hague LK, Lutz CS. (2007)
Specific trans-acting proteins interact with auxiliary RNA polyadenylation elements in the COX-2 3'-UTR.
RNA. 13(7):1103-1115.
 Sequences deriving from PTGS2 [5743] Western blot with HeLa NE
PSFUUAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUU-4Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
PSFUUUUUAAAUAUUUAUUUAUUUAUUUAUUUAUUUU-4Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
PSFGUUUUUAAUUUAUUUAUUAAGAUGGAUUCU-4Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
PSFAAACCUAUUUAUUAAUAUUUAAAACUAUUUAUAUG-4Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
PSFCUAAUGAUCAUAUUUAUUUAUUUAUAUGAACCAUG-4Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
PSFCCACGCACGCAGACUCGCAGACGCCCUCUGCUGGAACUGACACGCAGACAUUCAGCGGCU-5Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
PSFGAAGGA5Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 24632473Cho S, Moon H, Loh TJ, Oh HK, Williams DR, Liao DJ, Zhou J, Green MR, Zheng X, Shen H. (2014)
PSF contacts exon 7 of SMN2 pre-mRNA to promote exon 7 inclusion.
Biochim Biophys Acta.
 Construct of wt and mutant SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8In vivo splicing in 293A, C33A, SH-SY5Y along with PSF expression vectorRNA pulldown assay in HeLa nuclear extracts. In vivo splicing with deletion and mutation analysis.
PSFGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-10Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
PSFAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-1Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
PSFGAGGGAAC-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
PSFGAGAGGAA-2Gene Name and Synonymous: SFPQ, splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated), POMP100. 12403470Peng R, Dye BT, Perez I, Barnard DC, Thompson AB, Patton JG. (2002)
PSF and p54nrb bind a conserved stem in U5 snRNA.
RNA. 8(10):1334-1347.
 Sequences of 20nt random for SELEX. SELEX of random 20nt with recombinant protein.
QKICGGGGGCCUCAGUGUGCCAGCCUCCGGGCCCUAGCUGGGCUUCGGGGUUGGUG-5Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923.
QKI induces exon skipping [11917126]
11917126Wu JI, Reed RB, Grabowski PJ, Artzt K. (2002)
Function of quaking in myelination: regulation of alternative splicing.
Proc Natl Acad Sci U S A. 99(7):4233-4238.
 Sequences deriving from mouse Mag [17136]. EMSA supershift, competition assay, UV cross-linking with HeLa NE.
QKIAUUAAC-10Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923.
QKI induces exon skipping [11917126]
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
QKICUAAU-10Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923.
QKI induces exon skipping [11917126]
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
QKICGAGU-3Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923.
QKI induces exon skipping [11917126]
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
QKIACUUAU-1Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923.
QKI induces exon skipping [11917126]
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
QKIACUAAU-10Gene Name and Synonymous: QKI, QKI KH domain containing RNA binding, QK, Hqk, QK1, QK3, hqkI, DKFZp586I0923.
QKI induces exon skipping [11917126]
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
RBM25CGGGCA-5Gene Name and Synonymous: RBM25, RNA binding motif protein 25, S164, RNPC7, RED120, fSAP94, MGC105088, MGC117168.
RBM25 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 (PMID: 18663000).
18663000Zhou A, Ou AC, Cho A, Benz EJ Jr, Huang SC. (2008)
Novel splicing factor RBM25 modulates Bcl-x pre-mRNA 5' splice site selection.
Mol Cell Biol. 28(19): 5924-5936.
 Construct of Bcl-X [BCL2L1, 598] EX1 - INT1 - EX2 - INT2 - EX3 with wt or mutated EX2. Construct and variants of Adenovirus E1A unit.In vivo splicing assay in HeLa cellMutational analysis, immuoprecipitation assay in HeLa cells, EMSA and competition assay with recombinant protein.
RBM25AUCGGGCA-5Gene Name and Synonymous: RBM25, RNA binding motif protein 25, S164, RNPC7, RED120, fSAP94, MGC105088, MGC117168.
RBM25 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 (PMID: 18663000).
23190262Gong D, Yang F, Li F, Qian D, Wu M, Shao Z, Wu M, Wu J, Shi Y. (2013)
Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region.
Biochem J. 450(1):85-94.
 Synthetic sequence Fluorescence polarization assay
RBM4CCUUCCUU4Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
16260624Lin JC, Tarn WY. (2005)
Exon selection in alpha-tropomyosin mRNA is regulated by the antagonistic action of RBM4 and PTB.
Mol Cell Biol. 25(22): 10111-10121.
PTB and RBM4 are in competition for CU1 element. PTB appear to bind with more affinity than RBM4. Construct of alpha-TM [TPM1, 7168] EX8 - INT8 - EX9a - INT9a - EX9b and EX1 - INT1 - EX2b - INT2b - EX3.In vivo splicing in HEK293 cells.Mutational analysis.
RBM4UCCUUCUUG5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
16777844Kar A, Havlioglu N, Tarn WY, Wu JY. (2006)
RBM4 interacts with an intronic element and stimulates tau exon 10 inclusion.
J Biol Chem. 281(34): 24479-24488.
 WT and mutant constucts of Tau MAPT [4137] EX9 - INT9 - EX10 - INT10 - EX11.In vivo splicing in HEK293 cells.Mutational analysis, UV cross-linking assay with purified recombinant protein.
RBM4GCGCGCG5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
RBM4GCGCGGG5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
RBM4CGCGCGG5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
RBM4CGCGCGA5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
RBM4GCGCGGU5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
RBM4CCUCUUU-5Gene Name and Synonymous: RBM4, RNA binding motif protein 4, LARK, RBM4A, ZCRB3A, ZCCHC21, MGC75138, DKFZp547K0918.
RBM4 induce exon inclusion of alpha-TM EX9a and EX2b (PMID: 16260624) and tau EX10 (PMID: 16777844).
21518792Lin JC, Tarn WY. (2011)
RBM4 down-regulates PTB and antagonizes its activity in muscle cell-specific alternative splicing.
J Cell Biol. 193(3):509-520.
 Constructs of wt or mutated TPM1 [7168]_EX8 - Ptbp1 [19205]_INT10 - Ptbp1 [19205]_EX11 - Ptbp1 [19205]_INT11 - TPM1 [7168]_ EX9BIn vivo splicing in C2C12 cells overexpressing human recombinant proteinsMutagenesis, in vivo splicing assay
RBM5GGGGGGG-7Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 11029660Edamatsu H, Kaziro Y, Itoh H. (2000)
LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells.
Genes Cells. 5(10):849-858.
 Homopolymers Homopolymer binding assay with recombinant protein.
RBM5CCCCCCC-5Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 11029660Edamatsu H, Kaziro Y, Itoh H. (2000)
LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells.
Genes Cells. 5(10):849-858.
 Homopolymers Homopolymer binding assay with recombinant protein.
RBM5AAAAAAA-1Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 11029660Edamatsu H, Kaziro Y, Itoh H. (2000)
LUCA15, a putative tumour suppressor gene encoding an RNA-binding nuclear protein, is down-regulated in ras-transformed Rat-1 cells.
Genes Cells. 5(10):849-858.
 Homopolymers Homopolymer binding assay with recombinant protein.
RBM5CUCUUCUCU-5Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 18840686Fushimi K, Ray P, Kar A, Wang L, Sutherland LC, Wu JY. (2008)
Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5.
Proc Natl Acad Sci U S A. 105(41):15708-15713.
 Sequences deriving from mouse Casp2 [12366]. Constructs of wt and mutated mouse Casp2 [12366] EX8 - INT8 - EX9 - INT9 - EX10.In vitro splicing assay using HeLa NE and in vivo splicing in HEK293, protein overexpression and siRNA knockdown.EMSA and UV cross-linking with recombinant protein.
RBM5AGGUAA-5Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 22162216Farina B, Fattorusso R, Pellecchia M. (2011)
Targeting zinc finger domains with small molecules: solution structure and binding studies of the RanBP2-type zinc finger of RBM5.
Chembiochem. 12(18):2837-2845.
 Synthesized sequences Chemical shift mapping (NMR)
RBM5GGGGGG-10Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 22517726O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012)
Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA.
Angew Chem Int Ed Engl. 51(22):5371-5375.
 Synthesized sequences Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein
RBM5GGUGGU-3Gene Name and Synonymous: RBM5, RNA binding motif protein 5, G15, H37, RMB5, LUCA15, FLJ39876 22517726O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012)
Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA.
Angew Chem Int Ed Engl. 51(22):5371-5375.
 Synthesized sequences Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein
Sam68UUUUUUU6Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 17764653Rho J, Choi S, Jung CR, Im DS. (2007)
Arginine methylation of Sam68 and SLM proteins negatively regulates their poly(U) RNA binding activity.
Arch Biochem Biophys. 466(1):49-57.
Methylation of Sam68, SLM-1, SLM-2 proteins markedly reduced their poly(rU) binding ability in vitro. Homopolymers Immunoblot analysis with 293 and 293T lysates. Northwestern with recombinant protein.
Sam68UUAAAA5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 12490714Itoh M, Haga I, Li QH, Fujisawa J. (2002)
Identification of cellular mRNA targets for RNA-binding protein Sam68.
Nucleic Acids Res. 30(24):5452-5464.
 synthesized oligos Differential display and cDNA-representational difference analysis (cDNA-RDA) in vitro. In vivo coimmunoprecipitation. Further in vitro binding: protein incubated with labeled RNA.
Sam68CAAAAU2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68GAAAAC2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68UUUUUU5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 12490714Itoh M, Haga I, Li QH, Fujisawa J. (2002)
Identification of cellular mRNA targets for RNA-binding protein Sam68.
Nucleic Acids Res. 30(24):5452-5464.
 synthesized oligos Differential display and cDNA-representational difference analysis (cDNA-RDA) in vitro. In vivo coimmunoprecipitation. Further in vitro binding: protein incubated with labeled RNA.
Sam68UUUUA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 20186123Pedrotti S, Bielli P, Paronetto MP, Ciccosanti F, Fimia GM, Stamm S, Manley JL, Sette C. (2010)
The splicing regulator Sam68 binds to a novel exonic splicing silencer and functions in SMN2 alternative splicing in spinal muscular atrophy.
EMBO J. 29(7):1235-1247.
 Sequences deriving from SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HeLa, HEK293T, SH-SY5Y and SW480RNA pull-down assays with HEK293T NE, UV cross-linking and immunoprecipitation with HeLa and HEK293T NE, mutation analysis, EMSA and Western blot with HEK293T NE.
Sam68UUAAAU10Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68AUAAAA10Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68CUAAAU10Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68CUAAAA10Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68UUUUAC5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68AUUUAA5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68AUUUAC5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 9341174Lin Q, Taylor SJ, Shalloway D. (1997)
Specificity and determinants of Sam68 RNA binding. Implications for the biological function of K homology domains.
J Biol Chem. 272(43):27274-27280.
 Sequences of 40 nt random for SELEX. SELEX of 40nt random with recombinant protein. Winners and mutants confirmed by EMSA with recombinant protein.
Sam68CGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGG5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 20186122Sellier C, Rau F, Liu Y, Tassone F, Hukema RK, Gattoni R, Schneider A, Richard S, Willemsen R, Elliott DJ, Hagerman PJ, Charlet-Berguerand N. (2010)
Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients.
EMBO J. 29(7):1248-1261.
 Sequences deriving from FMR1 [2332]. FISH/IF and immunohistochemistry in human brain (hippocampal sections) of FXTAS patients.
Sam68AAAAUU5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 20876280Valacca C, Bonomi S, Buratti E, Pedrotti S, Baralle FE, Sette C, Ghigna C, Biamonti G. (2010)
Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene.
J Cell Biol. 191(1):87-99.
 Construct of SRSF1 [6426] EX4 - INT4 - EX5. Sequences deriving from SRSF1 [6426].In vivo splicing in SW480 cells, using WT or mutated minigenesMutational analysis and pull-down assay with HeLa nuclear extract. In vivo RNA immunoprecipitation of target RNA in cross-linked SW480 cells.
Sam68AUUAAA-10Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68UAAU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68AAAUAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68AAUAAU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68AUAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68UUAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68CUAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68AAAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68AUAAU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
Sam68UUAAUUAAUUAAUUAACUAACUAACUAACUUUAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 23637638Ehrmann I, Dalgliesh C, Liu Y, Danilenko M, Crosier M, Overman L, Arthur HM, Lindsay S, Clowry GJ, Venables JP, Fort P, Elliott DJ. (2013)
The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain.
PLoS Genet. 9(4):e1003474.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesEMSA with recombinant protein
Sam68AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUUAAUUAAUUAAUUAACCCACCCACCCACUUUAAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68UAAAAUAAAAUAAAAUAAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68ACAGUUUAAAAUUUGAUAAAAUUU-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68UUACAUUUAAAAGAUGAUUUAAAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUCCAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUUAAUCCAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUUAAUUAAUCCAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUUAAUUAAUUAAUCCACUAACUAACUAACUUUAAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUUAAUUAAUUAAUUAACUAACUAACUAACUUCCAAAACACGAUCUUAAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUCCAA-3Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
Sam68AAAAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAAAAAUAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAAU-2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAAUA-2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAAUAU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAAUUU-2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAUA-2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAUAA-2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAUAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAUAUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AAUUUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AUAA-2Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AUUAAUUA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68AUUUUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAAAUUUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAAU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAAUUUUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAUA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAUAAUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAUAUUUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAUUUUUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAUAAAUU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAUUAAU-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UUUAAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UUUAAAUAA-5Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
Sam68UAAAAGCGCCCGGUUUAAA-10Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26695943Rai DK, Lawrence P, Kloc A, Schafer E, Rieder E. (2015)
Analysis of the interaction between host factor Sam68 and viral elements during foot-and-mouth disease virus infections.
Virol J. 12:224.
 Sequences deriving from wt and mutated IRES of FMDV (foot-and-mouth disease virus) EMSA with recombinat protein
Sam68UACGAGCGCCCGGUUUACG-1Gene Name and Synonymous: KHDRBS1, KH domain containing RNA binding signal transduction associated 1, p62, FLJ34027. 26695943Rai DK, Lawrence P, Kloc A, Schafer E, Rieder E. (2015)
Analysis of the interaction between host factor Sam68 and viral elements during foot-and-mouth disease virus infections.
Virol J. 12:224.
 Sequences deriving from wt and mutated IRES of FMDV (foot-and-mouth disease virus) EMSA with recombinat protein
SAP155GAGGGAGGCAGGCGACGAG-5Gene Name and Synonymous: SF3B1, splicing factor 3b, subunit 1, 155kDa, PRP10, PRPF10, SAP155, SF3b155, SF3B1.
SAP155 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 in response to ceramide (PMID: 16790528).
16790528Massiello A, Roesser JR, Chalfant CE. (2006)
SAP155 Binds to ceramide-responsive RNA cis-element 1 and regulates the alternative 5' splice site selection of Bcl-x pre-mRNA.
FASEB J. 20(10): 1680-1682.
 Bcl-X [BCL2L1, 598] RNAs. Mass spectrometric analysis, EMSA supershift, RNAi, pull-down assay, western immunoblotting with A549 nuclear extract.
SAP155GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: SF3B1, splicing factor 3b, subunit 1, 155kDa, PRP10, PRPF10, SAP155, SF3b155, SF3B1.
SAP155 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 in response to ceramide (PMID: 16790528).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
SAP155AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: SF3B1, splicing factor 3b, subunit 1, 155kDa, PRP10, PRPF10, SAP155, SF3b155, SF3B1.
SAP155 stimulated proapoptotic Bcl-X(s) isoform through weak 5'ss selection in EX2 in response to ceramide (PMID: 16790528).
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
SC35AGAAG7Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
SC35AGCAG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
SC35AGGAG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
SC35AGUAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11847131Caputi M, Zahler AM. (2002)
SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D.
EMBO J. 21(4): 845-855.
 Construct of HIV-1 env [155971] EX_6D and part of flanking introns.In vitro splicing with HeLa nuclear extractsRNA affinity chromatography assay and immunoblot.
SC35AAGCAGUAGGG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AAGCAGUAGUC8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35ACGAGAG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35ACGAGAU8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGAGAUGCUG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGGAGAA8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGGAGAG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGGAGAU10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGGCAGU4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGGCAGUAGUC6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGGGUAU6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGUCGAU6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGUUCCAGCC4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AGUUCCAGGG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AUGAAUGCUG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGAACGAGAU4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35CGUGAUGCUG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GAGCAGUAGGG10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GAGCAGUAGUC10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GAGCAGUGGUC8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GAUGAUGCUG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GAUUCCAGCUA8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GAUUCCUGCUA10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GCGCAGUAGGG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GCGCAGUAGUC8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGAACCAGCUA4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGAAUGCCG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGAAUGCUG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGAGCC4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGCAGUAGGG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGUAGCUG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGUAUGCCG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGUAUGCUG10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGGUGAGCUG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGUGAUGCGU2Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGUUCCAGAU8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGUUCCAGUU8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GGUUCCUGUUA6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GUAACCAGCUA6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GUAUCCCGCUA6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GUGCAGUAGGG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GUUACCAGUCC2Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35GUUACGUGUAA4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UCGAGAU6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UCGUAAGCUG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGAUCGAGUA6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGGAGAU8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGGCAGUAGGG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCCAGAA8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCCAGAU10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCCAGCU8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCCAGUG8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCCGGAU8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCGAGAA8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCGAGAU10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCGAGUA8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35UGUUCGUGCG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SC35AAAAGAGAAG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SC35GAGGAGGA4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SC35GGAGGAC5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SC35GGAGGAG4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SC35GUAUUCUA7Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCCUCC5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCCCCUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGAUGGAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GACCGGUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35UGUUACUA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GACUAGAA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35AGCCUCAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCCCACA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGACGCUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35CGCUGCUA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GUUUCGAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GAGCACUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGUCGCCG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGUUAAUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCUGAUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCUCGUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGUCAGUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GAAUACCG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCUCCAA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGACUGUA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GACUCAAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GAUCCCCG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGAUCCGG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGUUGUUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GUCCUCCG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GAUCGCUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGCCGCAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGUUGGCG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35AGCUCCCA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GGACCGUA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GUCCUCAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GUCCCCUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GUCUAACG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35CGCCCUUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GUUCUGUA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GACCUGCG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35UGCUGUU5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9858550Schaal TD, Maniatis T. (1999)
Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA.
Mol Cell Biol. 19(1): 261-73.
 Synthesized oligos for UV-crosslink. Construct of beta-globin [3043] EX1 - INT1 - EX2. Construct and mutants of beta-globin [3043] EX3 - INT3 -partial_EX4 - EX2 for in vitro splicing.In vitro splicing in HeLa S100 extracts.UV crosslink and SDS-PAGE with HeLa S100 and nuclear extracts.
SC35UGCGGUC5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
SC35UGCCGCC5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
SC35ACGAGAGUU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGAAGCGGA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCAGAGAU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCAGAGGA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCAGAGUA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCAGUGUU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCCGAGAA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCGGAGAA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGAAUA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGAAUG3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGAUAA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGCGUG3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGUAUC3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGUGAC3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGCGAGCA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGCGAGUA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGCGUGUU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35CGGAGAGAA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GACGGAGUA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GAUGGAGGA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GCUCGAGUA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUACGAGGA3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUUCCAGAU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUUCCAGGU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUUCCAGUU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUUCGAGUG3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUUCGAGUU3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35UGCAGAGUG3Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGGAGAGGU6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35AGCAGAGUU10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUUCGAGUA10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SC35GUAAGUACGC8Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
14703516Zahler AM, Damgaard CK, Kjems J, Caputi M. (2004)
SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing.
J Biol Chem. 279(11): 10077-84.
 Constructs of HIV-1 Tat [155871] EX2.In Vitro Splicing with HeLa S100 or nuclear extracts.RNA Affinity Chromatography Assays, SDS-PAGE in HeLa cell nuclear extracts, Nuclear Extract Depletion by high affinity RNA. SDS-PAGE, immunoblotting, RNA Footprinting Analysis.
SC35CUAGACUAGA4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
14703516Zahler AM, Damgaard CK, Kjems J, Caputi M. (2004)
SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing.
J Biol Chem. 279(11): 10077-84.
 Constructs of HIV-1 Tat [155871] EX2.In Vitro Splicing with HeLa S100 or nuclear extracts.RNA Affinity Chromatography Assays, SDS-PAGE in HeLa cell nuclear extracts, Nuclear Extract Depletion by high affinity RNA. SDS-PAGE, immunoblotting, RNA Footprinting Analysis.
SC35AUCCCGUG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11779509Zhu J, Mayeda A, Krainer AR. (2001)
Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins.
Mol Cell. 8(6): 1351-1361.
 Sequence of HIV-1 Tat [155871] EX3.In Vitro Splicing with HeLa S100 and HeLa nuclear extracts.UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot.
SC35CACCUCCG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11779509Zhu J, Mayeda A, Krainer AR. (2001)
Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins.
Mol Cell. 8(6): 1351-1361.
 Sequence of HIV-1 Tat [155871] EX3.In Vitro Splicing with HeLa S100 and HeLa nuclear extracts.UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot.
SC35GAAUCCCG6Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11779509Zhu J, Mayeda A, Krainer AR. (2001)
Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins.
Mol Cell. 8(6): 1351-1361.
 Sequence of HIV-1 Tat [155871] EX3.In Vitro Splicing with HeLa S100 and HeLa nuclear extracts.UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot.
SC35GAUUAGUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11779509Zhu J, Mayeda A, Krainer AR. (2001)
Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins.
Mol Cell. 8(6): 1351-1361.
 Sequence of HIV-1 Tat [155871] EX3.In Vitro Splicing with HeLa S100 and HeLa nuclear extracts.UV crosslink, immunoprecipitation, affinity depletion, SDS-PAGE and Western blot.
SC35GGAGGA-5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
SC35GAAGAAGA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
14729981Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004)
RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.
Mol Cell Biol. 24(3):1387-1400.
 Sequences of fibronectin FN1 [2335] EX_EDA and mutants UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts
SC35AGGAGGA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35CGCAGGU5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35GACCCGG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35CGCAGGG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35CAGAGGU5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SC35AGCUGUU5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9858550Schaal TD, Maniatis T. (1999)
Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA.
Mol Cell Biol. 19(1): 261-73.
 Synthesized oligos for UV-crosslink. Construct of beta-globin [3043] EX1 - INT1 - EX2. Construct and mutants of beta-globin [3043] EX3 - INT3 -partial_EX4 - EX2 for in vitro splicing.In vitro splicing in HeLa S100 extracts.UV crosslink and SDS-PAGE with HeLa S100 and nuclear extracts.
SC35CAGUAGA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
14703516Zahler AM, Damgaard CK, Kjems J, Caputi M. (2004)
SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing.
J Biol Chem. 279(11): 10077-84.
 Constructs of HIV-1 Tat [155871] EX2.In Vitro Splicing with HeLa S100 or nuclear extracts.RNA Affinity Chromatography Assays, SDS-PAGE in HeLa cell nuclear extracts, Nuclear Extract Depletion by high affinity RNA. SDS-PAGE, immunoblotting, RNA Footprinting Analysis.
SC35GAUUGAUG-5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
19843576Chandradas S, Deikus G, Tardos JG, Bogdanov VY. (2010)
Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells.
J Leukoc Biol. 87(1):147-152.
In this context, SRp40 and SC35 antagonize other SR proteins by competing for certain sites in exon 5, thereby promoting TF (tissue factor) exon 5 exclusion. Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SC35GAUUCCCU-5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
19843576Chandradas S, Deikus G, Tardos JG, Bogdanov VY. (2010)
Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells.
J Leukoc Biol. 87(1):147-152.
In this context, SRp40 and SC35 antagonize other SR proteins by competing for certain sites in exon 5, thereby promoting TF (tissue factor) exon 5 exclusion. Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SC35CCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC-5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9130721Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997)
The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.
EMBO J. 16(7):1772-1784.
 Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. In vitro splicing with HeLa NEIn vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins.
SC35GAUCCCUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU-5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
9130721Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997)
The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.
EMBO J. 16(7):1772-1784.
 Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. In vitro splicing with HeLa NEIn vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins.
SC35AGAGGAAGGCGA7Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
SC35AGGAGCAGGGGACGAAG7Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
SC35GAAGAAGAA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
SC35AGGGUUGAGGGGAGCAGGGU7Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
SC35GGUGGGGCCGGGGCCAAGG-2Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
15798212Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005)
The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis.
Mol Cell Biol. 25(8):3286-3294.
 Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8In vitro splicing in HeLa NERNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA .
SC35GGUGGGGCCGGAGCCAAGG-10Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
15798212Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005)
The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis.
Mol Cell Biol. 25(8):3286-3294.
 Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8In vitro splicing in HeLa NERNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA .
SC35GAGGAG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
16990281Hallay H, Locker N, Ayadi L, Ropers D, Guittet E, Branlant C. (2006)
Biochemical and NMR study on the competition between proteins SC35, SRp40, and heterogeneous nuclear ribonucleoprotein A1 at the HIV-1 Tat exon 2 splicing site.
J Biol Chem. 281(48):37159-37174.
 Sequences deriving from HIV-1 Tat [155871] Competition assays with recombinant protein
SC35CGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
SC35CGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
SC35UGUGUCACUGUCUGGUUC1Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SC35GGCAGGAAGAAGAGGAGCA7Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SC35CCAGACACCGGAAACCCCUGCCACACCAC4Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SC35GGGACCUGCAGAGACUGUAA5Gene Name and Synonymous: SFRS2, splicing factor arginine/serine-rich 2, SC-35, SFRS2A, SRp30b, PR264.
SC35 accelerates transcriptional elongation (co-transcriptional splicing) (PMID: 18641664).
22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
SF1UACUAAC-10Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9182766Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997)
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
Cell. 89(5):781-787.
 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences.
SF1UGCUAAC-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9182766Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997)
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
Cell. 89(5):781-787.
 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences.
SF1GACUAAC-6Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9182766Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997)
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
Cell. 89(5):781-787.
 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences.
SF1UACUGAC-4Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9182766Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997)
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
Cell. 89(5):781-787.
 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences.
SF1UACUAAG-4Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9182766Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997)
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
Cell. 89(5):781-787.
 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences.
SF1UAGUAAG-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9182766Berglund JA, Chua K, Abovich N, Reed R, Rosbash M. (1997)
The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC.
Cell. 89(5):781-787.
 22 nt sequences containing yeast rp51A [854984] natural branchpoint sequence surrounded from the intron. EMSA, Filter Binding Assay using recombinant protein with WT and mutants sequences.
SF1UGCUGAC-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9512519Berglund JA, Abovich N, Rosbash M. (1998)
A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition.
Genes Dev. 12(6):858-867.
 Synthesized sequences Footprinting assay, EMSA, competition assay with purified protein
SF1UGCUGCC-1Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
9512519Berglund JA, Abovich N, Rosbash M. (1998)
A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition.
Genes Dev. 12(6):858-867.
 Synthesized sequences Footprinting assay, EMSA, competition assay with purified protein
SF1UACUAAU-10Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
11438677Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001)
Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex.
Mol Cell Biol. 21(15):5232-5241.
 Synthesized sequences Reporter gene assay using recombinant protein
SF1UAGUAAC-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
11438677Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001)
Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex.
Mol Cell Biol. 21(15):5232-5241.
 Synthesized sequences Reporter gene assay using recombinant protein
SF1UACUAGC-2Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
11438677Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001)
Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex.
Mol Cell Biol. 21(15):5232-5241.
 Synthesized sequences Reporter gene assay using recombinant protein
SF1UACGAAC-1Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
11438677Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001)
Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex.
Mol Cell Biol. 21(15):5232-5241.
 Synthesized sequences Reporter gene assay using recombinant protein
SF1ACAGUCA-2Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
11438677Peled-Zehavi H, Berglund JA, Rosbash M, Frankel AD. (2001)
Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex.
Mol Cell Biol. 21(15):5232-5241.
 Synthesized sequences Reporter gene assay using recombinant protein
SF1CACUGAC-7Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
15496424Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004)
Branch site haplotypes that control alternative splicing.
Hum Mol Genet. 13(24):3189-3202.
 Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 In vitro splicing in HeLa and HEK293T nuclear extracts.EMSA with recombinant protein.
SF1CGCUGAC-7Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
15496424Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004)
Branch site haplotypes that control alternative splicing.
Hum Mol Genet. 13(24):3189-3202.
 Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 In vitro splicing in HeLa and HEK293T nuclear extracts.EMSA with recombinant protein.
SF1CACCGAC-1Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
15496424Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004)
Branch site haplotypes that control alternative splicing.
Hum Mol Genet. 13(24):3189-3202.
 Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 In vitro splicing in HeLa and HEK293T nuclear extracts.EMSA with recombinant protein.
SF1CACAGAC-1Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
15496424Kralovicova J, Houngninou-Molango S, Kramer A, Vorechovsky I. (2004)
Branch site haplotypes that control alternative splicing.
Hum Mol Genet. 13(24):3189-3202.
 Construc of wt and mutant HLA-DQB1 [3119] EX3 - INT3 - EX4 - INT4 - EX5 - INT5 - EX6 In vitro splicing in HeLa and HEK293T nuclear extracts.EMSA with recombinant protein.
SF1AUUAAC-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
SF1ACUUAU-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
SF1ACUAAU-5Gene Name and Synonymous: SF1, splicing factor 1, ZFM1, ZNF162, D11S636.
Gomafu lncRNA UACUAAC repeats bind to mouse SF1 with a higher affinity than the mammalian branch point consensus regulating splicing efficiency by changing the splicing factors nuclear level (PMID: 21463453)
24722255Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J. (2014)
The RNA-Binding Protein QKI Suppresses Cancer-Associated Aberrant Splicing.
PLoS Genet. 10(4):e1004289.
 Construct of wt and mutant NUMB [8650] EX11 - INT11 - EX12 - INT12 - EX13. Constructs of wt and mutant Ad2 MINX EX1 - INT1 - EX2In vivo splicing in HEK-293 along with QKI expression vector. In vitro splicing assays in HeLa nuclear extracts and recombinant protein.EMSA with WT and mutated sequences and UV crosslinking with recombinant protein
SF2/ASFAACACGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
15302913Chiodi I, Corioni M, Giordano M, Valgardsdottir R, Ghigna C, Cobianchi F, Xu RM, Riva S, Biamonti G. (2004)
RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies.
Nucleic Acids Res. 32(14): 4127-4136.
 Construct of Adenovirus E1A for in vivo splicing. Synthesized 16nt oligos for EMSA.In vivo splicing in HeLa cells.EMSA.
SF2/ASFAAAGAAGAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11278454Chung H , Derse D. (2001)
Binding sites for Rev and ASF/SF2 map to a 55-nucleotide purine-rich exonic element in equine infectious anemia virus RNA.
J Biol Chem. 276 (22): 18960-18967.
 synthesized oligos EMSA, UV crosslink competition, and immunoprecipitation in HeLa nuclear extracts.
SF2/ASFAAAAGAGAAG4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SF2/ASFGAGGAGGA4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SF2/ASFGGAGGAC4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SF2/ASFGGAGGAG4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9848651Dye BT, Buvoli M, Mayer SA, Lin CH, Patton JG. (1998)
Enhancer elements activate the weak 3' splice site of alpha-tropomyosin exon 2.
RNA. 4(12): 1523-1536.
 Contructs of tropomyosin 1 alpha TPM1 [7168] EX1 - INT1 - EX2 - INT2 - EX3 with WT and mutants competitor sequences of TPM1 EX2 for in vitro splicing. Construct of TPM1 EX1 - INT1 - EX2 - INT2 - EX3 - INT3 - EX4 with wt or mutants EX2 for in vivo splicing.In vitro splicing and competition in HeLa cell nuclear extracts. In vivo splicing into smooth muscle cells (SMCs) and HeLa cells.UV crosslink, competition assay, Western blot, EMSA using EX2 alpha-TM and purified proteins or HeLa nuclear extracts.
SF2/ASFAGGAGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SF2/ASFCGCAGGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SF2/ASFGACCCGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
10629063Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. (2000)
Exonic splicing enhancer motif recognized by human SC35 under splicing conditions.
Mol Cell Biol. 20(3): 1063-1071.
 Constructs of mouse IgM EX_M1 - INT - EX_M2 mutated by inserting 20nt randomized in EX_M2. Construct of mouse IgM EX_C3 - INT - EX_C4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by in vitro splicing with HeLa nuclear extracts or with S100 extracts complemented by SC35. 
SF2/ASFCACAGUG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAGACGU10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAGAGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAGAGGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAGAGGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAGCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAGCGGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGCAGGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGCUCGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGGACGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGGACGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGGAGGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGGCCGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGGCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCUCAGGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCUCCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCUGACUA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGACAGGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFAGACAGAGAC4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
12419255Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, Branlant C. (2002)
A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding.
J Mol Biol. 323(4): 629-652.
 Constructs of HIV-1 A7 3' splice siteIn vitro splicing with HeLa cell nuclear or cytoplasmic S100 extracts.Competition assay, UV crosslink, immunoprecipitation with HeLa nuclear extracts, EMSA and supershift assays.
SF2/ASFGAAGAAGAA4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
12419255Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, Branlant C. (2002)
A Janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding.
J Mol Biol. 323(4): 629-652.
 Constructs of HIV-1 A7 3' splice siteIn vitro splicing with HeLa cell nuclear or cytoplasmic S100 extracts.Competition assay, UV crosslink, immunoprecipitation with HeLa nuclear extracts, EMSA and supershift assays.
SF2/ASFAGAAGAAC6Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGACAGAGC4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACAAGGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACGCGCC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGAAGGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGAUGGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGAAAGACC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGACGACGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGACUGAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGAGAAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGAGGAU2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGAGGUAAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAUGACAGAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAUGACUGAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFCCGCGCA2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFCGAAGGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFCGGACAGAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFCGGACGAAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGACGAAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGAUACGAAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGCGCGCA2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGGAAAGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGGAACAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGGAAUGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGGACGAAU2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGGAUGAAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGUGACAGAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUAGACAGAGC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUCGCACA2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUCGGGCA2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUGAAGAAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUGAUGAAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUGUAGGAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACGCGAG3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACGCGCU3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAUGACAGAAC3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFCAGACAGAGC3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGAGGAAC3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGGAGGAAC3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUAGACAGAAC3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUGGACAGAGC3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACGCGCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACGCUCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGACGGAAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGAGACAGAGC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFUUGACAGAGC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFACGCGCA7Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGAAGAGC7Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFAGGACGAAGC10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7543047Tacke R, Manley JL. (1995)
The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities.
EMBO J. 14(14): 3540-3551.
 Construct containing NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa nuclear and S100 extracts.SELEX of random 20nt with recombinant protein. Confirmed by EMSA, competition assay with recombinant protein and SELEX winners. UV-crosslinking and immunoprecipitation with Hela nuclear extract or S100 extract.
SF2/ASFGAGGAAGAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
7651409Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995)
A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer.
Mol Cell Biol. 15(9):4898-4907.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assay, immunoprecipitation
SF2/ASFAAGGUG2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
12612063Rooke N, Markovtsov V, Cagavi E, Black DL. (2003)
Roles for SR proteins and hnRNP A1 in the regulation of c-src exon N1.
Mol Cell Biol. 23(6):1874-1884.
 Construct of c-src [20779] EX_N1.In vitro splicing with Weri-1, Weri-1 S100 and HeLa nuclear extracts.UV crosslink and immunoprecipitation with Weri-1 and HeLa nuclear extracts.
SF2/ASFGGAAGAAGAUAAAGAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
12826680Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003)
The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20.
J Biol Chem. 278(35):32943-32953.
 Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10.In vivo splicing in SVK14 and HEK293-EBNA cells.UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts.
SF2/ASFGAUGAAGAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
10523623Bourgeois CF, Popielarz M, Hildwein G, Stevenin J. (1999)
Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation.
Mol Cell Biol. 19(11):7347-7356.
 Construct and variants of Adenovirus E1A unit.in vitro splicing with Hela nuclear extracts, in vivo splicing in Hela cells, in vitro complementation assays.UV crosslink, immunoprecipitation.
SF2/ASFAUCCAGGAGGGGAACAGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11751603Smith PJ, Spurrell EL, Coakley J, Hinds CJ, Ross RJ, Krainer AR, Chew SL. (2002)
An exonic splicing enhancer in human IGF-I pre-mRNA mediates recognition of alternative exon 5 by the serine-arginine protein splicing factor-2/alternative splicing factor.
Endocrinology. 143(1):146-154.
 Construct of IGF1 [3479] EX4 - INT4 - EX5.In vitro splicing with HeLa nuclear extracts and cotransfectionImmunoprecipitation.
SF2/ASFCAGACAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11925564Cartegni L, Krainer AR. (2002)
Disruption of an SF2/ASF-dependent exonic splicing enhancer in SMN2 causes spinal muscular atrophy in the absence of SMN1.
Nat Genet. 30(4):377-384.
 Construct and mutants of SMN1 [6606] and SMN2 [6607] EX6 - shortened_INT6 - EX7 - INT7 -shortened_EX8.In vivo splicing in 293-HEK.UV crosslink, immunoprecipitation, SDS-PAGE.
SF2/ASFGAAGAAGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
14729981Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004)
RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.
Mol Cell Biol. 24(3):1387-1400.
 Sequences of fibronectin FN1 [2335] EX_EDA and mutants UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts
SF2/ASFCAACCGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCACAGCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCACAGCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCACGGAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCAGUCGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCAACCC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCAACCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCAACGC10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCAAUGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCACAGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCACGCU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCACGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCACUGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCAUGGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCAGCC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCAUGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCCGAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCCGCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCGACU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCGUUU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCUGAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCCUGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGACAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGACCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGACGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGAGCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGAUGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGCUAU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCGGACG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCUAGGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCCUCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGACGCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGACGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGAGCGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGAGGCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGAUGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCCGGA10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCCGUA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGCAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGCCC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGGAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGGAC10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGGAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGGUU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGCGUCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGGAGCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGGAGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGGCACA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCGGCAGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCUACGAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCUCGUGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFCUGAACA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFGCCCACU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFGCCGGAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFUGAACCA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16825284Smith PJ, Zhang C, Wang J, Chew SL, Zhang MQ, Krainer AR. (2006)
An increased specificity score matrix for the prediction of SF2/ASF-specific exonic splicing enhancers.
Hum Mol Genet. 15(16):2490-2508.
 Construct of BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19 with random 7nt and 14nt inserted in EX18. Construct of SMN1 [6606] EX6 - INT6 - EX7 - INT7 - EX8 with 7nt SELEX-winners inserted in EX7.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs in HeLa S100 extract complemented by recombinant protein. Winners are confirmed by in vitro splicing in both HeLa nuclear extract and S100 extract complemented by recombinant protein.Functional SELEX of random 7nt and 14nt with recombinant protein.
SF2/ASFGACUGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGAGACGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGCACGG10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGGACGU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGUGACGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFAUAGGACUGGAUCGAGUUGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFAUCGGACAGGGUCCAGCAGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFAUCGGCCGAUCUGUGAGUUA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFAUGCUCCGGAAUCGGAACGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCAAGCACAGUGACCGAGAAC8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCCAGAGGGCGGAAACGUUGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGAUGACCCUCAGACGUAUA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGAUGUCCCGGAGGUUUUGC8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGCCGGACGACGUGUGUUG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGCGGUUAGGAGGAUGGAAA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGUCGCAGGGCAGGUGGGAA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGUGAAACUGCCCAGAGGUG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCGUGCCCACGUGUCUCAGGU8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFCUCCAGACGUCGUUUGUUGC8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGACGUCCAGUACGCUCGAGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGCGGACCCGGAAAGGACUAA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGAAGUACGGGACGUGCCGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGCACGGCGAGACACCAUCA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGCACGGGGAGGCACCAUCA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGCAGAGGAGAGCCGGGACG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGCAGCGGGCGUACCCGGAU8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGCUUGGUUCGCGGUGACGA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGGUCGCAGGUCAGGUGGGUU8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGUGGGUUCGGCGGAAUCAAG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFGUUGCGGAGACGACCCGAGC8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFUGACAGCGGAAGGUACAGUG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFUGAGUGCGCGGAUAGACUGACUA8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFUUCGGACGGGCUAGGGAUGG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SF2/ASFUGGAAAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
17144911Kammler S, Otte M, Hauber I, Kjems J, Hauber J, Schaal H. (2006)
The strength of the HIV-1 3' splice sites affects Rev function.
Retrovirology. 3:89.
 Constructs of HIV-1 Tat [155871] EX2.In vivo splicing in HeLa-T4+ cells.Mutational analysis and pull-down assay and immunoblot with HeLa nuclear extract.
SF2/ASFUAUUACGGAAU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19111562Goncalves V, Theisen P, Antunes O, Medeira A, Ramos JS, Jordan P, Isidro G. (2009)
A missense mutation in the APC tumor suppressor gene disrupts an ASF/SF2 splicing enhancer motif and causes pathogenic skipping of exon 14.
Mutat Res. 662(1-2): 33-36.
In this context, SF2/ASF and SRp55 do not bind UAUUAGGGAAU. In addiction, SRp55 does not bind UAUUACGGAAU. Construct of APC [324] INT13 - EX14 - INT14.In vivo splicing in DLD-1 or HT29 colorectal cells.RNA interference.
SF2/ASFGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
SF2/ASFGCGAGCG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SF2/ASFGAGCGAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SF2/ASFGAGCGGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SF2/ASFAGCGAGC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SF2/ASFCGGAAGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18315555Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008)
SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells.
J Thromb Haemost. 6(5):877-884.
 Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 and SC cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SF2/ASFCAGACAG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18315555Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008)
SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells.
J Thromb Haemost. 6(5):877-884.
 Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 and SC cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SF2/ASFGGAUAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFGCAUAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFGGAUUC7Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFGGGUAC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFGGAUAU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFCUGAUUUGUAUUUAUUAGACUC2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFAACAGAAAAAGAAAUAUUU2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFCUGAUUUGUACCUAUUAGAUUC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFUACUGAAGAACAAGUAUUU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SF2/ASFCCCCUCUCUCUAUCGCUGUCUCUUGAGCCACGC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9130721Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997)
The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.
EMBO J. 16(7):1772-1784.
 Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. In vitro splicing with HeLa NEIn vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins.
SF2/ASFGAUCCCUUUCUCUUUCUCUCUCCCUCCCUGUCUUUCCCU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9130721Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997)
The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.
EMBO J. 16(7):1772-1784.
 Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. In vitro splicing with HeLa NEIn vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins.
SF2/ASFAGGGAAAGACAGGGAGGGAGAGAGAAAGAGAAAGGGACU5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9130721Gallego ME, Gattoni R, Stevenin J, Marie J, Expert-Bezancon A. (1997)
The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.
EMBO J. 16(7):1772-1784.
 Construct of chicken TPM3 [396430] EX6A - INT6 - EX7. In vitro splicing with HeLa NEIn vitro splicing assay with RNA competitors, UV cross-linking both with HeLa extracts and recombinant proteins.
SF2/ASFAGAGCAGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
9826658Zheng ZM, Huynen M, Baker CC. (1998)
A pyrimidine-rich exonic splicing suppressor binds multiple RNA splicing factors and inhibits spliceosome assembly.
Proc Natl Acad Sci U S A. 95(24):14088-14093.
 BPV-1 sequences. UV cross-linking and immunoprecipitation with HeLa NE
SF2/ASFAGAGGAAGGCGA2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
SF2/ASFAGGAGCAGGGGACGAAG2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11096110Lejeune F, Cavaloc Y, Stevenin J. (2001)
Alternative splicing of intron 3 of the serine/arginine-rich protein 9G8 gene. Identification of flanking exonic splicing enhancers and involvement of 9G8 as a trans-acting factor.
J Biol Chem. 276(11):7850-7858.
 Sequences deriving from SFRS7 [6432] and constructs of SFRS7 [6432] EX3 - INT3 - EX4.In vitro splicing with HeLa extracts.UV cross-linking, immunoprecipitation, complementation assay in HeLa extracts.
SF2/ASFACAACAACUAGCCACGGAUCAU10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11336712Huang Y, Steitz JA. (2001)
Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA.
Mol Cell. 7(4):899-905.
 Sequences wt and mutated deriving from mouse Histone 2A UV cross-linking and immunoprecipitation with HeLa NE
SF2/ASFGGCGGCCAGAGGGUGUGCGGAGCUGGUGGGGAGGAGCCUGGAGAGAAGGGGCAGAGCUGGGGGCUGAGGGAGACCCCCCC10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11421362Hastings ML, Wilson CM, Munroe SH. (2001)
A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.
RNA. 7(6):859-874.
 Constructs of THRA [7067] EX7 - INT7 - EX8 - INT8 - EX9 - INT9 - EX10In vitro splicing with HeLa NEUV cross-linking, northwestern blotting, immunoprecipitation with HeLa NE and recombinant proteins.
SF2/ASFGGGGACCCGACAGGCCCGAAGGAAUAGAAGAAGAAGGUGGAGAGAGAGACAGAGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11575921Tange TO, Kjems J. (2001)
SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain.
J Mol Biol. 312(4):649-662.
 Constructs of wt and mutants of Tat [155871] EX2 - INT2 - EX3In vitro splicing with HeLa NE and recombinant proteinEMSA with recombinant protein, UV cross-linking with HeLa NE.
SF2/ASFAGGGUUGAGGGGAGCAGGGU7Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
15208309Expert-Bezancon A, Sureau A, Durosay P, Salesse R, Groeneveld H, Lecaer JP, Marie J. (2004)
hnRNP A1 and the SR proteins ASF/SF2 and SC35 have antagonistic functions in splicing of beta-tropomyosin exon 6B.
J Biol Chem. 279(37):38249-38259.
 Sequences deriving from TPM3 [396430] and synthesized sequences. Construct of chicken TPM3 [396430] EX6B - INT7 - EX7.In vitro splicing in HeLa NEEMSA with recombinant protein. RNA affinity chromatography and immunoprecipitation in HeLa NE
SF2/ASFGGUGGGGCCGGGGCCAAGG-5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
15798212Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005)
The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis.
Mol Cell Biol. 25(8):3286-3294.
 Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8In vitro splicing in HeLa NERNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA .
SF2/ASFGGUGGGGCCGGAGCCAAGG-5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
15798212Gabut M, Mine M, Marsac C, Brivet M, Tazi J, Soret J. (2005)
The SR protein SC35 is responsible for aberrant splicing of the E1alpha pyruvate dehydrogenase mRNA in a case of mental retardation with lactic acidosis.
Mol Cell Biol. 25(8):3286-3294.
 Construct of wt and mutated PDHA1 [5160] EX7-INT7-EX8In vitro splicing in HeLa NERNA affinity purification with HeLa NE, Western blot. UV cross-linking with HeLa NE or S100 or recombinant protein, siRNA .
SF2/ASFGGGAGGAAGAAAUAGAAGAUGCAGAAGAGG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
SF2/ASFGGUUUCAGACAAAAUCA10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16385450Cartegni L, Hastings ML, Calarco JA, de Stanchina E, Krainer AR. (2006)
Determinants of exon 7 splicing in the spinal muscular atrophy genes, SMN1 and SMN2.
Am J Hum Genet. 78(1):63-77.
 Constructs of SMN1 [6606] and SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8 In vitro splicing in HeLa RNA affinity chromatography with HeLa NE, western blot.
SF2/ASFUAUGGAGGAUUCACUGUACAGAAUGAAGCCAACAAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
16611940Spena S, Tenchini ML, Buratti E. (2006)
Cryptic splice site usage in exon 7 of the human fibrinogen Bbeta-chain gene is regulated by a naturally silent SF2/ASF binding site within this exon.
RNA. 12(6):948-958.
 Sequences deriving from FGB [2244] UV cross-linking, immunoprecipitation in HeLa NE
SF2/ASFCGAGAGCGUCGGUAUUAAGCGGGGGAGAAUUAGAUAAAUG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
SF2/ASFCGAGAGCGUCGGUAUUAAGCGGAUCCGAAUUAGAUAAAUG5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
17337441Schaub MC, Lopez SR, Caputi M. (2007)
Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes.
J Biol Chem. 282(18):13617-13626.
 Synthesized sequences RNA-affinity chromatography assays with HeLa NE and immunoblotting or with recombinant protein.
SF2/ASFUUACAUGAGCAUUAGGAGA-5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
17576688Buratti E, Stuani C, De Prato G, Baralle FE. (2007)
SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer.
Nucleic Acids Res. 35(13):4359-4368.
 Sequences deriving from CFTR [1080]. Constructs of CFTR [1080] INT8 - EX9 - INT9.In vivo splicing in Hep3BUV cross-linking, immunoprecipitation with HeLa NE
SF2/ASFCACACGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
17668007Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA. (2007)
Structural and functional analysis of RNA and TAP binding to SF2/ASF.
EMBO Rep. 8(8):756-762.
 Synthesized oligos UV cross-linking, chemical shift mapping (NMR) with recombinant protein.
SF2/ASFGCACCUGAUGGUGAAGAAGACACUGCAGAGC8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
SF2/ASFUGCAGAUGCUGAGUUUGUGU3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
SF2/ASFUGCAGAUGCUUAGUUUGUGU3Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18391021Goina E, Skoko N, Pagani F. (2008)
Binding of DAZAP1 and hnRNPA1/A2 to an exonic splicing silencer in a natural BRCA1 exon 18 mutant.
Mol Cell Biol. 28(11):3850-3860.
 Sequences deriving from FN1 [2335] and BRCA1 [672]. Constructs of wt and mutant BRCA1 [672] EX17 - INT17 - EX18 - INT18 - EX19.In vivo splicing in HeLa.Pulldown assay, western blot with HeLa NE and EMSA with recombinant protein. siRNA.
SF2/ASFGGCAGGAAGAAGAGGAGCA10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SF2/ASFCCAGACACCGGAAACCCCUGCCACACCAC4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SF2/ASFAAAGGACAAAGGACAAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
8769651Lynch KW, Maniatis T. (1996)
Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer.
Genes Dev. 10(16):2089-2101.
 Sequences deriving from D. melanogaster dsx [40940]. UV-cross link, immunoprecipitation with HeLa extracts.
SF2/ASFGACGACGAGGAGCAGCAG8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11454855Tian H, Kole R. (2001)
Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein.
J Biol Chem. 276(36):33833-33839.
 Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2).In vitro splicing with HeLa NEFilter binding assay, UV cross-linking and immunoprecipitation with HeLa NE
SF2/ASFGACGACUCAGCAG4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
11454855Tian H, Kole R. (2001)
Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein.
J Biol Chem. 276(36):33833-33839.
 Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2).In vitro splicing with HeLa NEFilter binding assay, UV cross-linking and immunoprecipitation with HeLa NE
SF2/ASFGUUGGAGAAACGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19602482Goncalves V, Matos P, Jordan P. (2009)
Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.
Hum Mol Genet. 18(19):3696-3707.
 Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4.In vivo splicing in HT29EMSA with recombinant protein and EMSA supershift with DLD-1 NE
SF2/ASFGUUGGACAAAUGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC1Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19602482Goncalves V, Matos P, Jordan P. (2009)
Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.
Hum Mol Genet. 18(19):3696-3707.
 Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4.In vivo splicing in HT29EMSA with recombinant protein and EMSA supershift with DLD-1 NE
SF2/ASFGUUGGAGAAACGUACGGCAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19602482Goncalves V, Matos P, Jordan P. (2009)
Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.
Hum Mol Genet. 18(19):3696-3707.
 Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4.In vivo splicing in HT29EMSA with recombinant protein and EMSA supershift with DLD-1 NE
SF2/ASFCAAAAAGAAGGAAGGUGCUCACAU10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19953646Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010)
A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy.
Hum Mutat. 31(1):E1110-1125.
 Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein
SF2/ASFCAAAAAGAACGAAGGUGCUCACAU4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
19953646Vezain M, Saugier-Veber P, Goina E, Touraine R, Manel V, Toutain A, Fehrenbach S, Frebourg T, Pagani F, Tosi M, Martins A. (2010)
A rare SMN2 variant in a previously unrecognized composite splicing regulatory element induces exon 7 inclusion and reduces the clinical severity of spinal muscular atrophy.
Hum Mutat. 31(1):E1110-1125.
 Sequences deriving from wt and mutated SMN2 [6607]. Constructs of SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293UV cross-linking and western blot with HeLa NE, EMSA with recombinant protein
SF2/ASFAUUUUCCUUACAGGGUUUUAGACAAAAUCAAAAAG2Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
SF2/ASFAUUUUCCUUACAGGGUUUCAGACAAAAUCAAAAAG10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
18794368Chen HH, Chang JG, Lu RM, Peng TY, Tarn WY. (2008)
The RNA binding protein hnRNP Q modulates the utilization of exon 7 in the survival motor neuron 2 (SMN2) gene.
Mol Cell Biol. 28(22):6929-6938.
 Sequences deriving from SMN1 [6606], SMN2 [6607]. Constructs of wt and mutated SMN2 [6607] EX6 - INT6 - EX7 - INT7 - EX8.In vivo splicing in HEK293.RNA affinity chromatography using HeLa NE, MALDI-TOF. RNA affinity chromatography using HEK293 extracts, immunoblotting, UV cross-linking. EMSA with recombinant protein.
SF2/ASFGAAUGGCCGGAGGAGAUUCAGCCUGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
20120036Homolova K, Zavadakova P, Doktor TK, Schroeder LD, Kozich V, Andresen BS. (2010)
The deep intronic c.903+469T>C mutation in the MTRR gene creates an SF2/ASF binding exonic splicing enhancer, which leads to pseudoexon activation and causes the cblE type of homocystinuria.
Hum Mutat. 31(4):437-444.
 Sequences deriving from wt or mutated MTRR [4552]. Constructs of wt or mutated HBB [3043]_EX1 - MTRR [4552]_INT6 - HBB [3043]_EX2 and Tat [155871]_EX1 - MTRR [4552]_INT6 - Tat [155871]_EX2.In vivo splicing in HEK293, protein overexpression and siRNA knockdownPulldown and Western blotting with HeLa NE
SF2/ASFUGCUUUGGUUAUAUUUAGCUCCAAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
20118245Moulton VR, Tsokos GC. (2010)
Alternative splicing factor/splicing factor 2 regulates the expression of the zeta subunit of the human T cell receptor-associated CD3 complex.
J Biol Chem. 285(17):12490-12496.
 Sequences deriving from CD247 [919] and synthesized sequencesIn vivo splicing in T cells overexpressing SF2/ASFEMSA supershift with human T cells NE or recombinant protein
SF2/ASFGGGACCUGCAGAGACUGUAA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
22434879Zubovic L, Baralle M, Baralle FE. (2012)
Mutually exclusive splicing regulates the Nav 1.6 sodium channel function through a combinatorial mechanism that involves three distinct splicing regulatory elements and their ligands.
Nucleic Acids Res. 40(13):6255-6269.
 Sequences deriving from SCN8A [6334]. Constructs of wt or mutated SCN8A [6334] EX17 - INT17 - EX18N - INT18 - EX18A - INT18 - EX19In vivo splicing in HeLaPulldown, mass spectrophotometry, western blot and siRNA knockdown with HeLa.
SF2/ASFACGCCCUCUGCUGGAACUGA5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
20122404Motta-Mena LB, Heyd F, Lynch KW. (2010)
Context-dependent regulatory mechanism of the splicing factor hnRNP L.
Mol Cell. 37(2):223-234.
 Constructs of wt or mutated PTPRC [5788] EX3 - INT3 - EX4 - INT4 - EX7 and EX3 - INT3 - EX5 - INT5 - EX7.In vitro splicing in JSL1 NEMutational analysis. EMSA with recombinant proteins or JSL1 NE. Pull-down and western blotting with JSL1 NE.
SF2/ASFGGGGCCGGGGCCGGGGCCGGGGCC8Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
23423380Reddy K, Zamiri B, Stanley SY, Macgregor RB Jr, Pearson CE. (2013)
The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures.
J Biol Chem. 288(14):9860-9866.
 Synthetic sequences. EMSA with recombinant protein.
SF2/ASFAGAAGAACAGAAGAACAGAAGAAC4Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
23423380Reddy K, Zamiri B, Stanley SY, Macgregor RB Jr, Pearson CE. (2013)
The disease-associated r(GGGGCC)n repeat from the C9orf72 gene forms tract length-dependent uni- and multimolecular RNA G-quadruplex structures.
J Biol Chem. 288(14):9860-9866.
 Synthetic sequences. EMSA with recombinant protein.
SF2/ASFGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
24371143Zamiri B, Reddy K, Macgregor RB Jr, Pearson CE. (2014) TMPyP4 porphyrin distorts RNA G-quadruplex structures of the disease-associated r(GGGGCC)n repeat of the C9orf72 gene and blocks interaction of RNA-binding proteins. J Biol Chem. 289(8):4653-4659.  Synthetic sequences. EMSA with recombinant protein.
SF2/ASFCAGGUAAGAC5Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
8566754Lou H, Gagel RF, Berget SM. (1996)
An intron enhancer recognized by splicing factors activates polyadenylation.
Genes Dev. 10(2):208-219.
 Sequences deriving from wt and mutated intron 4 of CALCA [796]  UV crosslink and immunoprecipitation
SF2/ASFGCAGUGACGACGCGCUGCUCAAGAACUACGGUCUGCUCUCCUGCUUCCGGAAGGACCUGCAUAAGACGGAGACGUACCUGAGGGUCAUGAAGUGCCGCCGCUUCGGGGAGGCCAG10Gene Name and Synonymous: SFRS1, splicing factor arginine/serine-rich 1 (splicing factor 2, alternate splicing factor), ASF, SF2, SF2p33, SRp30a, MGC5228.
The shuttling protein SF2/ASF binds TAP and can function as export factors (18364396).
8276242Sun Q, Mayeda A, Hampson RK, Krainer AR, Rottman FM. (1993)
General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer.
Genes Dev. 7(12B):2598-2608.
 Construct of cow GH1 [280804] EX4 - INT4 - EX5In vitro splicing in HeLa nuclear extract, using also competing RNAs or protein excessUV crosslinking and immunoprecipitation in HeLa nuclear extract, UV crosslinking with purified HeLa protein or recombinant protein
SLM-1UUUUUUU7Gene Name and Synonymous: KHDRBS2, KH domain containing RNA binding signal transduction associated 2, SLM1, FLJ38664, MGC26664, bA535F17.1. 17764653Rho J, Choi S, Jung CR, Im DS. (2007)
Arginine methylation of Sam68 and SLM proteins negatively regulates their poly(U) RNA binding activity.
Arch Biochem Biophys. 466(1):49-57.
Methylation of Sam68, SLM-1, SLM-2 proteins markedly reduced their poly(rU) binding ability in vitro. Homopolymers Immunoblot analysis with 293 and 293T lysates. Northwestern with recombinant protein.
SLM-2UUUUUUU7Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 17764653Rho J, Choi S, Jung CR, Im DS. (2007)
Arginine methylation of Sam68 and SLM proteins negatively regulates their poly(U) RNA binding activity.
Arch Biochem Biophys. 466(1):49-57.
Methylation of Sam68, SLM-1, SLM-2 proteins markedly reduced their poly(rU) binding ability in vitro. Homopolymers Immunoblot analysis with 293 and 293T lysates. Northwestern with recombinant protein.
SLM-2AGAUAAA5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SLM-2AUAAAAC5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SLM-2UAAAUAA5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SLM-2AAAUAAA5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SLM-2AUUAAAC5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SLM-2AUUAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2UAAU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2AAAUAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2AAUAAU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2AUAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2UUAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2CUAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2AAAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2AUAAU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2ACAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2AUAAC-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 26758068Feracci M, Foot JN, Grellscheid SN, Danilenko M, Stehle R, Gonchar O, Kang HS, Dalgliesh C, Meyer NH, Liu Y, Lahat A, Sattler M, Eperon IC, Elliott DJ, Dominguez C. (2016)
Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.
Nat Commun. 7:10355.
 Synthetic sequences. X-ray crystallography and fluorescence polarization
SLM-2UUAAUUAAUUAAUUAACUAACUAACUAACUUUAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 23637638Ehrmann I, Dalgliesh C, Liu Y, Danilenko M, Crosier M, Overman L, Arthur HM, Lindsay S, Clowry GJ, Venables JP, Fort P, Elliott DJ. (2013)
The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain.
PLoS Genet. 9(4):e1003474.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesEMSA with recombinant protein
SLM-2AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUUAAUUAAUUAAUUAACCCACCCACCCACUUUAAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2UAAAAUAAAAUAAAAUAAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2ACAGUUUAAAAUUUGAUAAAAUUU-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2UUACAUUUAAAAGAUGAUUUAAAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUCCAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUUAAUCCAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUUAAUUAAUCCAUUAACUAACUAACUAACUUUAAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUUAAUUAAUUAAUCCACUAACUAACUAACUUUAAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUUAAUUAAUUAAUUAACUAACUAACUAACUUCCAAAACACGAUCUUAAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAUUAAUUAAUUAAUUAACUAACUAACUAACUUUAAAAACACGAUCUCCAA-10Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 27994030Danilenko M, Dalgliesh C, Pagliarini V, Naro C, Ehrmann I, Feracci M, Kheirollahi-Chadegani M, Tyson-Capper A, Clowry GJ, Fort P, Dominguez C, Sette C, Elliott DJ. (2016)
Binding site density enables paralog-specific activity of SLM2 and Sam68 proteins in Neurexin2 AS4 splicing control.
Nucleic Acids Res.
 Construct of mouse Nrxn2 [18190] INT19 - EX20 - INT20In vivo splicing in HEK293 cells, using WT or mutated minigenesFluorescence polarization
SLM-2AAAAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAAAAAUAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAAU-2Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAAUA-2Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAAUAU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAAUUU-2Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAUA-2Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAUAA-2Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAUAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAUAUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AAUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AUAA-2Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AUUAAUUA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2AUUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAAAUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAAU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAAUUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAUA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAUAAUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAUAUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAAUUUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAUAAAUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UAAUUAAU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UUUAAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UUUAAAUAA-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SLM-2UUUUUU-5Gene Name and Synonymous: KHDRBS3, KH domain containing RNA binding signal transduction associated 3, Etle, SALP, SLM2, TSTAR, T-STAR, etoile. 24096002Foot JN, Feracci M, Dominguez C. (2014)
Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.
Methods. 65(3):288-301.
 Synthetic sequences. X-ray crystallography, NMR spectroscopy and NMR chemical shift
SRm160GGGAACAAAAGCUGGGUACCGGGCCCCCCCUCGA5Gene Name and Synonymous: SRRM1, serine/arginine repetitive matrix 1, POP101, SRM160, MGC39488, 160-KD. 12600940Szymczyna BR, Bowman J, McCracken S, Pineda-Lucena A, Lu Y, Cox B, Lambermon M, Graveley BR, Arrowsmith CH, Blencowe BJ. (2003)
Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.
Genes Dev. 17(4):461-475.
 Synthesized sequences EMSA supershift using recombinant protein
SRp20ACAUCAUCU6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACAUCGACU10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACAUCGAUC9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACAUCGAUU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACAUCGCUU8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACAUCGUUC8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACUACGACG4Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACUACGAUC6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACUUCGACU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CGAUCGACU6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAACGACU8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAACGAUC8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAACGAUU8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAGAGAUU6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAUCGACA8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAUCGACU10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAUCGAUA8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAUCGAUC10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAUCGCUU8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCAUCGUUC8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCUUCGAUC9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20AACUUUAU6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20ACAUUCAU4Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20AUCAUCAU6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20AUCUUCAC6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20AUCUUCAU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CACAUCAU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CACUACAC6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CACUUCAG8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CACUUCAU10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CGCUUCAU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUCUUCAC8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20GACUUCAU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20GACUUCUU6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UACAUCAC6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UACUUCAA8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UUCAUCAC6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UUCAUCAU9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UUCUCCAA6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UUCUUCAA9Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UUCUUCAU10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CAUCAAC8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUACAAA8Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUACAAC10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUACAAU7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUACAGC7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUACGAC7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUUCAAC10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20GAUCAAC3Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UAUCAAC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UCACAAC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UGUCAAC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20UUACGAC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10094314Cavaloc Y, Bourgeois CF, Kister L, Stevenin J. (1999)
The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.
RNA. 5(3): 468-483.
 Sequences of 20 nt random for SELEX. Constructs of EXE1A_Adenovirus - partial_INT_E1A_Adenovirus - partial_INT_FN1 - EX_ED1_FN1 of FIBRONECTIN (FN1) [2335] for in vitro splicing. Construct of Sp1 unit of Adenovirus E1A for in vitro splicing. In vitro splicing in HeLa S100 extracts.SELEX of 20-nt random with recombinant protein. Winners confirmed by EMSA with recombinant protein, UV crosslink, complementation assay, immunoprecipitations with HeLa S100 and nuclear extracts.
SRp20CUCCGCUCCUCUUC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
9710581Lou H, Neugebauer KM, Gagel RF, Berget SM. (1998)
Regulation of alternative polyadenylation by U1 snRNPs and SRp20.
Mol Cell Biol. 18(9): 4977-85.
 Construct of CALCA [796] INT4 and mutants for UV-crosslink and immunoprecipitation. Construct of CALCA EX4 - INT4 - EX5 - INT5 - EX6 fused to a heterologous first exon from adenovirus used for in vivo splicing.In vivo splicing in T98G cells.UV crosslink, competition, immunoprecipitation in HeLa cell nuclear extracts. EMSA with recombinant protein.
SRp20CCUCGUCC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
10022858Schaal TD, Maniatis T. (1999)
Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.
Mol Cell Biol. 19(3): 1705-1719.
 Constructs of Drosophila dsx [40940] EX3 - INT3 - EX4 mutated by inserting 18nt randomized in EX4.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa nuclear extracts. Winners are confirmed by in vitro splicing in HeLa S100 extracts. SELEX winner confermed by immublotting in Hela nuclear extracts.
SRp20GGAAGAAGAUAAAGAC3Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
12826680Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003)
The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20.
J Biol Chem. 278(35):32943-32953.
 Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10.In vivo splicing in SVK14 and HEK293-EBNA cells.UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts.
SRp20UUCUUCUUCUUCUUCUUCUUCUUCUUCUUC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
SRp20ACAACAAGAAGACGCGCAUCAU5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
11336712Huang Y, Steitz JA. (2001)
Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA.
Mol Cell. 7(4):899-905.
 Sequences wt and mutated deriving from mouse Histone 2A UV cross-linking and immunoprecipitation with HeLa NE
SRp20CUGCACCACCACCUGGUUC2Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp20CUGCACCACCACCUAUCUA6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp20CCAGACACCGGAAACCCCUGCCACACCAC6Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp20GUUGGAGAAACGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC-10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
19602482Goncalves V, Matos P, Jordan P. (2009)
Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.
Hum Mol Genet. 18(19):3696-3707.
 Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4.In vivo splicing in HT29EMSA with recombinant protein and EMSA supershift with DLD-1 NE
SRp20GUUGGACAAAUGUACGGUAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC-10Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
19602482Goncalves V, Matos P, Jordan P. (2009)
Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.
Hum Mol Genet. 18(19):3696-3707.
 Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4.In vivo splicing in HT29EMSA with recombinant protein and EMSA supershift with DLD-1 NE
SRp20GUUGGAGAAACGUACGGCAAGGAUAUAACCUCCCGGGGCAAAGACAAGCCGAUUGCC-1Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
19602482Goncalves V, Matos P, Jordan P. (2009)
Antagonistic SR proteins regulate alternative splicing of tumor-related Rac1b downstream of the PI3-kinase and Wnt pathways.
Hum Mol Genet. 18(19):3696-3707.
 Sequences deriving from RAC1 [5879]. Construcs of RAC1 [5879] EX3 - INT3 - EX3b - INT3 - EX4.In vivo splicing in HT29EMSA with recombinant protein and EMSA supershift with DLD-1 NE
SRp20CCUCUUCC7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20CUUCAAC7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20UCUUCAC7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20CAUCAU7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20CUUCAC7Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20UCAUC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20UUCAC5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20CAUC3Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20GAUC1Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
17036044Hargous Y, Hautbergue GM, Tintaru AM, Skrisovska L, Golovanov AP, Stevenin J, Lian LY, Wilson SA, Allain FH. (2006)
Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8.
EMBO J. 25(21):5126-5137.
 Synthesized sequences NMR spectroscopy
SRp20UUCUUCAUCC-5Gene Name and Synonymous: SFRS3, splicing factor arginine/serine-rich 3.
The shuttling protein SRp20 binds TAP and can function as export factors (18364396).
24321384Jang HN, Lee M, Loh TJ, Choi SW, Oh HK, Moon H, Cho S, Hong SE, Kim do H, Sheng Z, Green MR, Park D, Zheng X, Shen H. (2014)
Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRSF3 through interaction with exon 8.
Biochim Biophys Acta. 1839(1):25-32.
 Construct of CASP2 [835] EX8 - INT8 - EX9 - INT9 - EX10In vivo splicing in MDA-MB-231 and HeLaIn vivo splicing with deletion and mutation analysis. RNA pulldown assay in HeLa nuclear extracts.
SRp30cAAGAC1Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cACCAC3Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGAAC1Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGCAC2Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGCAG5Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGGAA2Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGGAC10Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGGAG4Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGGAU1Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cAGGCA3Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cCGGAC1Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cCGGAG1Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cCGGUG1Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cUGGAC2Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cUGGAU2Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 17548433Paradis C, Cloutier P, Shkreta L, Toutant J, Klarskov K, Chabot B. (2007)
hnRNP I/PTB can antagonize the splicing repressor activity of SRp30c.
RNA 13: 1287-1300.
 Synthesized oligos. Sequences of 20nt random for SELEX.In vitro splicing with HeLa nuclear extracts, siRNA.SELEX of 20nt random with recombinant protein. EMSA, UV crosslink, SDS-PAGE with HeLa nuclear extracts.
SRp30cCUGGAUU-5Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 12024014Simard MJ, Chabot B. (2002)
SRp30c is a repressor of 3' splice site utilization.
Mol Cell Biol. 22(12): 4001-4010.
In this context, SRp30c has splicing repressor activity. Construct of Adenovirus Major late EX_L1 - INT - EX_L2 with 3 copies of wt ISE or mutants inserted into the intron.In vitro splicing with HeLa nuclear extract.RNA affinity chromatography, mutation analysis and EMSA with HeLa nuclear extract.
SRp30cGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA5Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
SRp30cUGAGUCGGAUCGCAGCUUGGAUGGCCAC5Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 18534987Cloutier P, Toutant J, Shkreta L, Goekjian S, Revil T, Chabot B. (2008)
Antagonistic effects of the SRp30c protein and cryptic 5' splice sites on the alternative splicing of the apoptotic regulator Bcl-x.
J Biol Chem. 283(31):21315-21324.
 Construct of BCL2L1 [600039] EX1-EX2-INT2-EX3In vitro splicing assays in HeLa nuclear extracts and recombinant proteinEMSA using recombinant protein. UV cross-linking in HeLa and immunoprecipitation.
SRp30cAGAUGGGCAAGGAGGUGGA5Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 16249178Park E, Han J, Son GH, Lee MS, Chung S, Park SH, Park K, Lee KH, Choi S, Seong JY, Kim K. (2006)
Cooperative actions of Tra2alpha with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript.
J Biol Chem. 281(1):401-409.
 Sequences deriving from mouse Gnrh1 [14714]. Construct of Gnrh1 [14714] EX1 - INT1 - EX2 - EX3 - EX4.In vitro splicing in HeLa NEEMSA, UV cross-linking with recombinant protein
SRp30cGAAAGUCUGAUUGAAGAGGAAGCCAGGCAGAAGAAGA5Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 16249178Park E, Han J, Son GH, Lee MS, Chung S, Park SH, Park K, Lee KH, Choi S, Seong JY, Kim K. (2006)
Cooperative actions of Tra2alpha with 9G8 and SRp30c in the RNA splicing of the gonadotropin-releasing hormone gene transcript.
J Biol Chem. 281(1):401-409.
 Sequences deriving from mouse Gnrh1 [14714]. Construct of Gnrh1 [14714] EX1 - INT1 - EX2 - EX3 - EX4.In vitro splicing in HeLa NEEMSA, UV cross-linking with recombinant protein
SRp30cCCAGACACCGGAAACCCCUGCCACACCAC4Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp30cGACGACGAGGAGCAGCAG8Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 11454855Tian H, Kole R. (2001)
Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein.
J Biol Chem. 276(36):33833-33839.
 Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2).In vitro splicing with HeLa NEFilter binding assay, UV cross-linking and immunoprecipitation with HeLa NE
SRp30cGACGACUCAGCAG4Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 11454855Tian H, Kole R. (2001)
Strong RNA splicing enhancers identified by a modified method of cycled selection interact with SR protein.
J Biol Chem. 276(36):33833-33839.
 Synthesized sequences and sequences deriving from S. cerevisiae URA3 [856692]. Constructs of URA3 [856692] EX1 - INT1 - EX2 - INT2 - EX3 (sequence inserted in EX2).In vitro splicing with HeLa NEFilter binding assay, UV cross-linking and immunoprecipitation with HeLa NE
SRp30cGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC2Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
SRp30cAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC2Gene Name and Synonymous: SFRS9, splicing factor arginine/serine-rich 9. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
SRp38GACAAA4Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
18794844Feng Y, Chen M, Manley JL. (2008)
Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator.
Nat Struct Mol Biol. 15(10):1040-1048.
 Constructs of beta-globin [3043].In vitro splicing with HeLa S100 extracts. Splicing-inhibition and spliceosome-assembly assays.EMSA and RNase protection assay with recombinant protein.
SRp38AGACAA7Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
18794844Feng Y, Chen M, Manley JL. (2008)
Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator.
Nat Struct Mol Biol. 15(10):1040-1048.
 Constructs of beta-globin [3043].In vitro splicing with HeLa S100 extracts. Splicing-inhibition and spliceosome-assembly assays.EMSA and RNase protection assay with recombinant protein.
SRp38AAAGACAAA7Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
18794844Feng Y, Chen M, Manley JL. (2008)
Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator.
Nat Struct Mol Biol. 15(10):1040-1048.
 Constructs of beta-globin [3043].In vitro splicing with HeLa S100 extracts. Splicing-inhibition and spliceosome-assembly assays.EMSA and RNase protection assay with recombinant protein.
SRp38ACAAAGACAAA5Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
12419250Shin C, Manley JL. (2002)
The SR protein SRp38 represses splicing in M phase cells.
Cell. 111(3):407-417.
 Sequences of 20 nt random for SELEX. SELEX of random 20nt with recombinant protein with mitotic phase HeLa extracts, Western Blot.
SRp38AAAGAAA5Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SRp38GAAAGAA5Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SRp38AAAGAAG5Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SRp38AAAAGAA5Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SRp38AGAGAAA5Gene Name and Synonymous: FUSIP1, FUS interacting protein (serine/arginine-rich) 1, NSSR, TASR, SRp38, TASR1, TASR2, FUSIP2, SFRS13, SRrp40.
Dephosphorylation converts SRp38 to a splicing repressor (PMID: 12419250)
SRp38 is an atypical SR protein that functions as a general splicing repressor when dephosphorylated, but when phosphorylated it functions as a sequence-specific splicing activator (PMID: 18794844).
19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
SRp40AAAGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACACC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACACG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACAGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACAGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACCGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACGGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACGGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACUGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACUGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40AGAGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GCAGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GCAGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GCUGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UCCGC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UCGGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UCUGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CCGGAGGGGUCGGCUCGU2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UAGGAGGCUGGAUCGU2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UGAGAAGAUAGCACCA2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UGGGAGCAGUCGGCUCGA2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UGGGAGCUGAGCUCGC2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UUGGAGUAACAAGGAGUA2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UGGGAGCAGUCGGCUCAU3Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40CCGGAGGGGUUAGUUCCC5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UGGGAGCAAAGCUCGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40UGGGAGCAGUCGGCUCGU10Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9037021Tacke R, Chen Y, Manley JL. (1997)
Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer.
Proc Natl Acad Sci U S A. 94(4): 1148-1153.
 Construct of NCAM1 [4684] EX18, downstream 5' splice site, alpha globin HBA2 [3040] INT2, HBA2 EX3. Sequences of 20 nt random for SELEX.In vitro splicing in HeLa cell nuclear extracts.SELEX of 20nt random with recombinant protein, confirmed by UV crosslink, EMSA, immunoblot in HeLa nuclear extracts.
SRp40GAGGAAGAA5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 7651409Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995)
A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer.
Mol Cell Biol. 15(9):4898-4907.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assay, immunoprecipitation
SRp40GAAGAAGA5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 14729981Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004)
RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.
Mol Cell Biol. 24(3):1387-1400.
 Sequences of fibronectin FN1 [2335] EX_EDA and mutants UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts
SRp40AACACGGCUGUGAGUGGUCC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACAUGAACACAACGUCGGGG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACCAGGGUCGUCCGUCUGGG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACGCUCAAUAGAAAUCAAG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40ACGGGCGGACUCCUCUGGUA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40AGACCAGUAGCCGCUGCCGG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40AUGGGUCUGACACGCUGACU8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CAGGGCACUUGUUUCACUGG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CCAAUCGGAUCACCUAACGGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CCUCACUGGACUCAGUGGUG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CGACGUGUGGGGACGGCAAG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CGAGGAAUAUAAAGGUGGGA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40CUUGAGGUGAAGGUCAUGUG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GAAAGUUGUAAAGACAGGGG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GAACCUUGCAGGUCGCGCGA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GACGUUGGUGUUAUCCGCCA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GCAGUGACUGCAUUGGCAGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GGCGUUUUCGAGGAUCGGGA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GGUAAGUACUACAGGGUGUG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GUAGGACUGGAUCGCGUUGG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GUAUUUCGACACCAGUGUGA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GUGAUACAUACAGGUGGCGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UAGUAACCGCGACAGUAGGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UCUAAGGCGCUAAGAACGGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UGCAGAGGAUAGCCGGAACG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UGCUCACCCGGCCGCCACAGC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UGGAUGUCAGCGACGGGCCA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UGUGCAGCUUGCGUCACGUC8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40UUACAACUGCACCACGGUCG8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp40GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
SRp40GGAAGAAGAUAAAGAC3Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 12826680Galiana-Arnoux D, Lejeune F, Gesnel MC, Stevenin J, Breathnach R, Del Gatto-Konczak F. (2003)
The CD44 alternative v9 exon contains a splicing enhancer responsive to the SR proteins 9G8, ASF/SF2, and SRp20.
J Biol Chem. 278(35):32943-32953.
 Construct of CD44 [960] EX8 - INT8 - EX9 - INT9 - EX10.In vivo splicing in SVK14 and HEK293-EBNA cells.UV crosslink, immunoprecipitation in HeLa S100 and nuclear extracts.
SRp40AUAAAGG-5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 19843576Chandradas S, Deikus G, Tardos JG, Bogdanov VY. (2010)
Antagonistic roles of four SR proteins in the biosynthesis of alternatively spliced tissue factor transcripts in monocytic cells.
J Leukoc Biol. 87(1):147-152.
In this context, SRp40 and SC35 antagonize other SR proteins by competing for certain sites in exon 5, thereby promoting TF (tissue factor) exon 5 exclusion. Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SRp40CUGAUUUGUACCUAUUAGAUUC2Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp40UACUGAAGAACAAGUAUUU5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp40GGGAGGAAGAAAUAGAAGAUGCAGAAGAGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 16000324Venables JP, Bourgeois CF, Dalgliesh C, Kister L, Stevenin J, Elliott DJ. (2005)
Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon.
Hum Mol Genet. 14(16):2289-22303.
 Constructs of wt or mutated HBB [3043]_EX2 - HIPK3 [10114]_INT3 - HIPK3 [10114]_EX3bis - HIPK3 [10114]_INT3 - HBB [3043]_ EX3. Sequences deriving from HIPK3 [10114].In vivo splicing in HEK293 cells.Pulldown assay and western blot with HeLa NE
SRp40GGAGAGAAAGAAGGGAAGAUGAGAGG5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 16254078Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005)
Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene.
Nucleic Acids Res. 33(18):6000-6010.
 Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3In vivo splicing in Hep3BPull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA.
SRp40GGUAUUUUUGGAGAAAUUC-8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 17576688Buratti E, Stuani C, De Prato G, Baralle FE. (2007)
SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer.
Nucleic Acids Res. 35(13):4359-4368.
 Sequences deriving from CFTR [1080]. Constructs of CFTR [1080] INT8 - EX9 - INT9.In vivo splicing in Hep3BUV cross-linking, immunoprecipitation with HeLa NE
SRp40UGUAUAAUAGAAAUUGUUC-5Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 17576688Buratti E, Stuani C, De Prato G, Baralle FE. (2007)
SR protein-mediated inhibition of CFTR exon 9 inclusion: molecular characterization of the intronic splicing silencer.
Nucleic Acids Res. 35(13):4359-4368.
 Sequences deriving from CFTR [1080]. Constructs of CFTR [1080] INT8 - EX9 - INT9.In vivo splicing in Hep3BUV cross-linking, immunoprecipitation with HeLa NE
SRp40GGCAGGAAGAAGAGGAGCA8Gene Name and Synonymous: SFRS5, splicing factor arginine/serine-rich 5, HRS. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp54AAGAAG-5Gene Name and Synonymous: SFRS11, splicing factor, arginine/serine-rich 11, p54. 16943417Wu JY, Kar A, Kuo D, Yu B, Havlioglu N. (2006)
SRp54 (SFRS11), a regulator for tau exon 10 alternative splicing identified by an expression cloning strategy.
Mol Cell Biol. 26(18):6739-6747.
 Construct of Tau MAPT [4137] EX9 - INT9 - EX10 - INT10 - EX11 with GFP cDNA inserted into EX11.In vivo splicing in HEK293.Positive clones identified by fluorescence-activated cell sorting and visual inspection. Confirmed by UV crosslink, immunoprecipitation, SDS-PAGE.
SRp55ACCGGG5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55ACCGUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55AGCGGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55AUCGUA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CACGGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CCCGGC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGCGUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GACGUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GCCGGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GCCGUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UACGUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UCCGGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UGCGGC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UGCGUA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UGCGUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UGCGUG5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GAGGAAGAA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 7651409Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995)
A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer.
Mol Cell Biol. 15(9):4898-4907.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assay, immunoprecipitation
SRp55AAGAGGAAGAAUGGCUUGAGGAAGACGACG5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9436904Nagel RJ, Lancaster AM, Zahler AM. (1998)
Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55.
RNA. 4(1):11-23.
 Sequences of Gallus gallus cardiac troponin T TNNT2 [396433] EX5 and mutants for EMSA. Construct of beta-globin [3043] EX1 - INT1 - EX2 for in vitro splicing.In vitro splicing with HeLa S100 extracts, also in competition with EX5 and mutants of TNNT2.EMSA with purified protein. Hill plot analysis. Chemical modification interference with kethoxal.
SRp55GCAGCACCUGGC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 12549914Tran Q, Roesser JR. (2003)
SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing.
Biochemistry. 42(4):951-957.
 Construct of calcitonin/calcitonin gene-related peptide CALCA [796] EX3 - INT3 - EX4 - INT4 - EX5.In vitro splicing with HeLa and HEK 293 cells nuclear extracts.EMSA, UV crosslink.
SRp55GAAGAAGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 14729981Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004)
RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.
Mol Cell Biol. 24(3):1387-1400.
 Sequences of fibronectin FN1 [2335] EX_EDA and mutants UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts
SRp55AGACCGUCAACAUGUCUGCC8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55AUAGCGAGCGGAAAACAGGUAA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55AUGCAGACGAUGGUGCGGCU8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CAAACCGUCAAAGUACGUCA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CAAACCUGCGUGGUAUGGUA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CACCAGCGGAGUCCCCAGAGC8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CAUGAAGCCGUCACCAACGUCUAG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGAGCCACGGACCACACGGA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGAUUCAGGUACGUCCAACU8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGCACACUGCGUCCCGGGGC8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGCGUCGUGUCGUAGGGGGC8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGCGUGCGUGCAGUGCCAAG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55CGUGUCGCGUCCUCGUGUGC8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GACCGGGAUUGAAGGAGCU8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GACGUCGCCCCGUGUGUAAG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GAGUUGAGCGAUGGUGCGUA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GAUCGAAUCCGGAACACAGG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GAUCGUAUCCGGAACACGGG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GGAUAACGGUGUGGCCCGGC8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GGAUCGAAUCCGGAACACGG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GGAUCGUAAGUGCAGACGA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GGAUCGUAAGUGCAGAUGA8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GGCCGGACGCAUUGCAGAG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55UCCGAUCUGUGCACGGACG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9649504Liu HX, Zhang M, Krainer AR. (1998)
Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins.
Genes Dev. 12(13): 1998-2012.
 Construct of EX_M1 - INT1 - EX_M2 murine IgM and its variants obtained by replacing the natural ESE in the M2 exon with 20nt random.SELEX imposing a selection of the constructs for splicing rather than for binding. Selection of the constructs by splicing in HeLa S100 extracts complemented by recombinant SR protein. Winners are confirmed by in vitro splicing in HeLa nuclear extracts.UV crosslink, competition and immunoprecipitation assays in HeLa nuclear extracts.
SRp55GAAGAAGAAGAAGAAGAAGAAGAAGAAGAA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 18597733Baralle M, Pastor T, Bussani E, Pagani F. (2008)
Influence of Friedreich ataxia GAA noncoding repeat expansions on pre-mRNA processing.
Am J Hum Genet. 83(1): 77-88.
 Synthesized sequences. Mass spectrometry, immunoblot analysis.
SRp55AAGGGAGGGAAUGGCUUGAGGAAGACGACG2Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9436904Nagel RJ, Lancaster AM, Zahler AM. (1998)
Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55.
RNA. 4(1):11-23.
 Sequences of Gallus gallus cardiac troponin T TNNT2 [396433] EX5 and mutants for EMSA. Construct of beta-globin [3043] EX1 - INT1 - EX2 for in vitro splicing.In vitro splicing with HeLa S100 extracts, also in competition with EX5 and mutants of TNNT2.EMSA with purified protein. Hill plot analysis. Chemical modification interference with kethoxal.
SRp55AAGAGGAAGAAGAAGAAGAGGAAGACGACG8Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 9436904Nagel RJ, Lancaster AM, Zahler AM. (1998)
Specific binding of an exonic splicing enhancer by the pre-mRNA splicing factor SRp55.
RNA. 4(1):11-23.
 Sequences of Gallus gallus cardiac troponin T TNNT2 [396433] EX5 and mutants for EMSA. Construct of beta-globin [3043] EX1 - INT1 - EX2 for in vitro splicing.In vitro splicing with HeLa S100 extracts, also in competition with EX5 and mutants of TNNT2.EMSA with purified protein. Hill plot analysis. Chemical modification interference with kethoxal.
SRp55GAUCAACCUGGC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 12549914Tran Q, Roesser JR. (2003)
SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing.
Biochemistry. 42(4):951-957.
 Construct of calcitonin/calcitonin gene-related peptide CALCA [796] EX3 - INT3 - EX4 - INT4 - EX5.In vitro splicing with HeLa and HEK 293 cells nuclear extracts.EMSA, UV crosslink.
SRp55GCUCAUCCUGGC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 12549914Tran Q, Roesser JR. (2003)
SRp55 is a regulator of calcitonin/CGRP alternative RNA splicing.
Biochemistry. 42(4):951-957.
 Construct of calcitonin/calcitonin gene-related peptide CALCA [796] EX3 - INT3 - EX4 - INT4 - EX5.In vitro splicing with HeLa and HEK 293 cells nuclear extracts.EMSA, UV crosslink.
SRp55UGUGGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 18315555Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008)
SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells.
J Thromb Haemost. 6(5):877-884.
 Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 and SC cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SRp55UGCGGA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 18315555Tardos JG, Eisenreich A, Deikus G, Bechhofer DH, Chandradas S, Zafar U, Rauch U, Bogdanov VY. (2008)
SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells.
J Thromb Haemost. 6(5):877-884.
 Construct of F3 [2152] EX4-INT4-EX5-INT5-EX6 and mutantsIn vivo splicing in THP-1 and SC cells.Mutagenesis, splicing assays, EMSA, Immunoblot.
SRp55UUAGACUCUCCUUUUGGAUACCUAGAUGUUUUAACA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55UUAGACUCUCCUUUUGCAUACCUAGAUGUUUUAACA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55UUAGACUCUCCUUUUGGAUUCCUAGAUGUUUUAACA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55UUAGACUCCCCUUUUGGAUACCUAGAUGUUUUAACA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55UUAGACUCUCCUUUUGGGUACCUAGAUGUUUUAACA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55UUAGACUCUCCUUUUGGAUAUCUAGAUGUUUUAACA5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55CUGAUUUGUAUUUAUUAGACUC1Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55AACAGAAAAAGAAAUAUUU2Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55CUGAUUUGUACCUAUUAGAUUC5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55UACUGAAGAACAAGUAUUU5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp55GGAGAGAAAGAAGGGAAGAUGAGAGG5Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 16254078Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005)
Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene.
Nucleic Acids Res. 33(18):6000-6010.
 Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3In vivo splicing in Hep3BPull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA.
SRp55GGCAGGAAGAAGAGGAGCA3Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp55CCAGACACCGGAAACCCCUGCCACACCAC2Gene Name and Synonymous: SFRS6, splicing factor arginine/serine-rich 6, B52, MGC5045. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp75GAGGAAGAA5Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 7651409Ramchatesingh J, Zahler AM, Neugebauer KM, Roth MB, Cooper TA. (1995)
A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer.
Mol Cell Biol. 15(9):4898-4907.
 Construct of cardiac troponin T TNNT2 [7139] EX4 - INT4 - EX5In vitro splicing in HeLa nuclear extracts.UV crosslink, competition assay, immunoprecipitation
SRp75GAAGAAGA5Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 14729981Buratti E, Muro AF, Giombi M, Gherbassi D, Iaconcig A, Baralle FE. (2004)
RNA folding affects the recruitment of SR proteins by mouse and human polypurinic enhancer elements in the fibronectin EDA exon.
Mol Cell Biol. 24(3):1387-1400.
 Sequences of fibronectin FN1 [2335] EX_EDA and mutants UV crosslink, competition assay, immunoprecipitation with HeLa nuclear extracts
SRp75GGACAGCAGAGAUCCAGU5Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 18272582Exline CM, Feng Z, Stoltzfus CM. (2008)
Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression.
J Virol. 82(8): 3921-3931.
 Constructs of Drosophila dsx [40940] EX3-INT3-20bpEX4 adding wt or mutated ESE at EX4.In vitro-splicing with HeLa nuclear extract.UV cross-linking and immunoprecipitation in HeLa nuclear extract.
SRp75CUGAUUUGUACCUAUUAGAUUC2Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp75UACUGAAGAACAAGUAUUU2Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 19910374Haque A, Buratti E, Baralle FE. (2010)
Functional properties and evolutionary splicing constraints on a composite exonic regulatory element of splicing in CFTR exon 12.
Nucleic Acids Res. 38(2):647-659.
 Constructs of CFTR [1080] INT11-EX12-INT12. Synthesized sequences.In vivo splicing assay in HeLa cells.Mutagenesis, western blot, siRNA knockdown
SRp75UGUGUCACUGUCUGGUUC3Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp75GGCAGGAAGAAGAGGAGCA8Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
SRp75CCAGACACCGGAAACCCCUGCCACACCAC2Gene Name and Synonymous: SFRS4, splicing factor arginine/serine-rich 4. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
TDP43UGUGUGUGUG-3Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
15826655Ayala YM, Pantano S, D'Ambrogio A, Buratti E, Brindisi A, Marchetti C, Romano M, Baralle FE. (2005)
Human, Drosophila, and C.elegans TDP43: nucleic acid binding properties and splicing regulatory function.
J Mol Biol. 348(3):575-588.
 Construct of tropomyosin 1 alpha TPM1 [7168] EX2 and EX3 separated by a synthetic intron with the 5' end partially corresponding to beta-globin [3043].In vitro splicing with HeLa nuclear extracts.UV crosslinking, EMSA.
TDP43UGUGUGUGUGUG-4Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
11470789Buratti E, Baralle FE. (2001)
Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9.
J Biol Chem. 276(39):36337-36343.
 synthesized oligos UV crosslink, SDS-PAGE, EMSA with recombinant protein.
TDP43UGUGUGUGUGUGUGUGUG-6Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
11470789Buratti E, Baralle FE. (2001)
Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9.
J Biol Chem. 276(39):36337-36343.
 synthesized oligos UV crosslink, SDS-PAGE, EMSA with recombinant protein.
TDP43UGUGUGUGUGUGUGUGUGUGUG-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
15826655Ayala YM, Pantano S, D'Ambrogio A, Buratti E, Brindisi A, Marchetti C, Romano M, Baralle FE. (2005)
Human, Drosophila, and C.elegans TDP43: nucleic acid binding properties and splicing regulatory function.
J Mol Biol. 348(3):575-588.
 Construct of tropomyosin 1 alpha TPM1 [7168] EX2 and EX3 separated by a synthetic intron with the 5' end partially corresponding to beta-globin [3043].In vitro splicing with HeLa nuclear extracts.UV crosslinking, EMSA.
TDP43UGUGUGUGUGUGUGUGUGUGUGUGUGUUU-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
16458894Ayala YM, Pagani F, Baralle FE. (2006)
TDP43 depletion rescues aberrant CFTR exon 9 skipping.
FEBS Lett. 580(5):1339-1344.
 Constructs of CFTR [1080] EX9 along with part of the flanking introns.In vivo splicing in HeLa, Hep3B, lymphoblasts and EBV transformed lymphocytes.Western blot, siRNA.
TDP43UGUGUG-2Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
11470789Buratti E, Baralle FE. (2001)
Characterization and functional implications of the RNA binding properties of nuclear factor TDP-43, a novel splicing regulator of CFTR exon 9.
J Biol Chem. 276(39):36337-36343.
 synthesized oligos UV crosslink, SDS-PAGE, EMSA with recombinant protein.
TDP43UGUGUGUG-2Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
15826655Ayala YM, Pantano S, D'Ambrogio A, Buratti E, Brindisi A, Marchetti C, Romano M, Baralle FE. (2005)
Human, Drosophila, and C.elegans TDP43: nucleic acid binding properties and splicing regulatory function.
J Mol Biol. 348(3):575-588.
 Construct of tropomyosin 1 alpha TPM1 [7168] EX2 and EX3 separated by a synthetic intron with the 5' end partially corresponding to beta-globin [3043].In vitro splicing with HeLa nuclear extracts.UV crosslinking, EMSA.
TDP43GUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGU-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
16254078Mercado PA, Ayala YM, Romano M, Buratti E, Baralle FE. (2005)
Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene.
Nucleic Acids Res. 33(18):6000-6010.
 Construct of heterologous exons of alpha-globin, fibronectin and APOA2 [336] INT2 - EX3 - INT3In vivo splicing in Hep3BPull-down assay in HeLa NE, western blot. UV cross-linking, immunoprecipitation in HeLa NE, siRNA.
TDP43UGUGUGUGUGUGUGUGUGUGUGUUUUU-1Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
TDP43UGUGUGUGUGUGUGUGUGUGUGUGUUUUU-2Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
TDP43UGUGUGUGUGUGUGUGUGUGUGUGUGUUUUU-3Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
20631008Dujardin G, Buratti E, Charlet-Berguerand N, Martins de Araujo M, Mbopda A, Le Jossic-Corcos C, Pagani F, Ferec C, Corcos L. (2010)
CELF proteins regulate CFTR pre-mRNA splicing: essential role of the divergent domain of ETR-3.
Nucleic Acids Res. 38(20):7273-7285.
 Constructs of wt and mutated CFTR [1080] INT8 - EX9 - INT9In vivo splicing in HeLa, HGT-1, HCT116EMSA with recombinant protein. siRNA.
TDP43UGAGGUAGUAGGUUGUGUGGUU-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
20423455Buratti E, De Conti L, Stuani C, Romano M, Baralle M, Baralle F. (2010)
Nuclear factor TDP-43 can affect selected microRNA levels.
FEBS J. 277(10):2268-2281.
 Sequences deriving from MIRLET7B [406884] and mutated MIRLET7A1 [406881], mutated MIRLET7A2 [406882], mutated MIRLET7A3 [406883]. EMSA with recombinant protein
TDP43UGAGGUAGUAGGUUGUGUAGUU-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
20423455Buratti E, De Conti L, Stuani C, Romano M, Baralle M, Baralle F. (2010)
Nuclear factor TDP-43 can affect selected microRNA levels.
FEBS J. 277(10):2268-2281.
 Sequences deriving from MIRLET7B [406884] and mutated MIRLET7A1 [406881], mutated MIRLET7A2 [406882], mutated MIRLET7A3 [406883]. EMSA with recombinant protein
TDP43GUUGUGC-10Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
19815002Volkening K, Leystra-Lantz C, Yang W, Jaffee H, Strong MJ. (2009)
Tar DNA binding protein of 43 kDa (TDP-43), 14-3-3 proteins and copper/zinc superoxide dismutase (SOD1) interact to modulate NFL mRNA stability. Implications for altered RNA processing in amyotrophic lateral sclerosis (ALS).
Brain Res. 1305:168-182.
 Sequences deriving fromwt and mutated NEFL [4747] Immunoprecipitation in HEK293 cells.
TDP43GUUUUGC-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
19815002Volkening K, Leystra-Lantz C, Yang W, Jaffee H, Strong MJ. (2009)
Tar DNA binding protein of 43 kDa (TDP-43), 14-3-3 proteins and copper/zinc superoxide dismutase (SOD1) interact to modulate NFL mRNA stability. Implications for altered RNA processing in amyotrophic lateral sclerosis (ALS).
Brain Res. 1305:168-182.
 Sequences deriving fromwt and mutated NEFL [4747] Immunoprecipitation in HEK293 cells.
TDP43GUUGUUC-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
19815002Volkening K, Leystra-Lantz C, Yang W, Jaffee H, Strong MJ. (2009)
Tar DNA binding protein of 43 kDa (TDP-43), 14-3-3 proteins and copper/zinc superoxide dismutase (SOD1) interact to modulate NFL mRNA stability. Implications for altered RNA processing in amyotrophic lateral sclerosis (ALS).
Brain Res. 1305:168-182.
 Sequences deriving fromwt and mutated NEFL [4747] Immunoprecipitation in HEK293 cells.
TDP43UGUGUGUGUGUGUGUGUGUGUGUG-5Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
16157593Buratti E, Brindisi A, Giombi M, Tisminetzky S, Ayala YM, Baralle FE. (2005)
TDP-43 binds heterogeneous nuclear ribonucleoprotein A/B through its C-terminal tail: an important region for the inhibition of cystic fibrosis transmembrane conductance regulator exon 9 splicing.
J Biol Chem. 280(45):37572-37584.
 Synthesized sequences EMSA with recombinant protein
TDP43GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC-2Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
TDP43AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC-2Gene Name and Synonymous: TARDBP, TAR DNA binding protein, ALS10.
It can act as transcriptional repressor (21252238)
23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
TIA-1UUUUUUAUUUU-5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17035636Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006)
A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators.
Mol Biol Cell. 17(12):5105-5114.
 Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts.
TIA-1UUUUUUUUUUUAGUUGCUAAUUUAUUGUUUU8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 11106748Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J. (2000)
The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing.
Mol Cell. 6(5): 1089-1098.
In this context, TIA-1 promotes exon 6 inclusion. Synthesized sequences. Construct of Drosophila msl-2 [33565] INT1 and flanking sequences. Construct of human FAS [355] EX5 - INT5 - EX6 - INT6 - EX7.In vitro splicing of msl-2 construct and reconstitution assay. In vivo splicing of FAS construct in mouse fibroblast Bk6 cell line overexpressing human TIA-1UV-crosslink, Filter binding assay, Mutational analysis, Immunoprecipitation with HeLa nuclear extract.
TIA-1UUCAUUUUUGAUUCUUUGGUUAAAAAA-5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 19339348Choi EY, Pintel D. (2009)
Splicing of the large intron present in the non-structural gene of minute virus of mice (MVM) is governed by TIA-1/TIAR binding downstream of the non-consensus donor.
J Virol. 83(12):6306-6311
 Synthesized sequences. RNA chromatography affinity and immunoblotting in HeLa nuclear extract.
TIA-1UUAUUUUUUUACGGUUAUAUUCUCCUUUCCC5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 11106748Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J. (2000)
The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing.
Mol Cell. 6(5): 1089-1098.
In this context, TIA-1 promotes exon 6 inclusion. Synthesized sequences. Construct of Drosophila msl-2 [33565] INT1 and flanking sequences. Construct of human FAS [355] EX5 - INT5 - EX6 - INT6 - EX7.In vitro splicing of msl-2 construct and reconstitution assay. In vivo splicing of FAS construct in mouse fibroblast Bk6 cell line overexpressing human TIA-1UV-crosslink, Filter binding assay, Mutational analysis, Immunoprecipitation with HeLa nuclear extract.
TIA-1UGUGUGUGUGUAGUUGCUAAUUUAUUGUUUU4Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 11106748Forch P, Puig O, Kedersha N, Martinez C, Granneman S, Seraphin B, Anderson P, Valcarcel J. (2000)
The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing.
Mol Cell. 6(5): 1089-1098.
In this context, TIA-1 promotes exon 6 inclusion. Synthesized sequences. Construct of Drosophila msl-2 [33565] INT1 and flanking sequences. Construct of human FAS [355] EX5 - INT5 - EX6 - INT6 - EX7.In vitro splicing of msl-2 construct and reconstitution assay. In vivo splicing of FAS construct in mouse fibroblast Bk6 cell line overexpressing human TIA-1UV-crosslink, Filter binding assay, Mutational analysis, Immunoprecipitation with HeLa nuclear extract.
TIA-1AUUUA1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1AUUUC4Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1AUUUUC1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1AUUUUG2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUA2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUC10Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUG2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUUA2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUUC5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUUG2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUUUA4Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1CUUUUUC3Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUA2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUC5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUG4Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUUA1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUUC2Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUUG1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUUUC1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUUUG1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1GUUUUUUUUC1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIA-1UCUUGCUUUGUUCAAAC5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 16109372Izquierdo JM, Majos N, Bonnal S, Martinez C, Castelo R, Guigo R, Bilbao D, Valcarcel J. (2005)
Regulation of Fas alternative splicing by antagonistic effects of TIA-1 and PTB on exon definition.
Mol Cell. 19(4):475-484.
 Sequences deriving from wt and mutated FAS [355]. Construct of FAS [355] EX5 - INT5 - EX6 - INT6 - EX7In vitro splicing in HeLa NEUV cross-linking, immunoprecipitation with HeLa NE
TIA-1UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG-5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 16890199Izquierdo JM. (2006)
Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR.
Biochem Biophys Res Commun. 348(2):703-711.
 Sequences deriving from ATP5B [506] UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein.
TIA-1UAUUGCUUGGGUAAUUUUUUUUUUUAGUUGCUAAUUUAUUG8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17488725Izquierdo JM, Valcarcel J. (2007)
Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein.
J Biol Chem. 282(27):19410-19417.
 Sequences deriving from FAS [355] and Drosophila msl-2 [33565] EMSA with recombinant protein
TIA-1GCCAAUUCCACUAAUUGUUUGGGGUAAGUUCUUGCUUUGUUCAAACUGCAGAUUGAAA4Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17488725Izquierdo JM, Valcarcel J. (2007)
Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein.
J Biol Chem. 282(27):19410-19417.
 Sequences deriving from FAS [355] and Drosophila msl-2 [33565] EMSA with recombinant protein
TIA-1GUAAUUUAUUUAUUUCCUGUUCAACAUAAAUAAAUUAC5Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17580305McAlinden A, Liang L, Mukudai Y, Imamura T, Sandell LJ. (2007)
Nuclear protein TIA-1 regulates COL2A1 alternative splicing and interacts with precursor mRNA and genomic DNA.
J Biol Chem. 282(33):24444-24454.
 Sequences deriving from COL2A1 [1280]. Constructs of COL2A1 [1280] EX1 - INT1 - EX2 - INT2 - EX3.In vivo splicing in TC28UV cross-linking with recombinant protein
TIA-1UUAUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUU-8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
TIA-1UUUUUAAAUAUUUAUUUAUUUAUUUAUUUAUUUU-8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
TIA-1GUUUUUAAUUUAUUUAUUAAGAUGGAUUCU-8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
TIA-1AAACCUAUUUAUUAAUAUUUAAAACUAUUUAUAUG-8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
TIA-1CUAAUGAUCAUAUUUAUUUAUUUAUAUGAACCAUG-8Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 17965020Buxade M, Morrice N, Krebs DL, Proud CG. (2008)
The PSF.p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha.
J Biol Chem. 283(1):57-65.
 Sequences deriving from FOS [2353], PTGS2 [5743] and mouse Tnf [21926], Csf2 [12981], Ifng [15978]. Pull-down, western blot with Jurkat extracts.
TIA-1GUUAUAUUCUCCUUUC10Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 24682828Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. (2014) Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res.  Sequences deriving from FAS [355]. Synthetic sequences. NMR, isothermal titration calorimetry and SAXS.
TIA-1UUCUCCUUUC1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 24682828Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. (2014) Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res.  Sequences deriving from FAS [355]. Synthetic sequences. NMR, isothermal titration calorimetry and SAXS.
TIA-1UUUUUUUUU1Gene Name and Synonymous: TIA1, cytotoxic granule-associated RNA binding protein. 24682828Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. (2014) Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res.  Sequences deriving from FAS [355]. Synthetic sequences. NMR, isothermal titration calorimetry and SAXS.
TIAL1UUUUUUAUUUU-5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 17035636Zhu H, Hasman RA, Barron VA, Luo G, Lou H. (2006)
A nuclear function of Hu proteins as neuron-specific alternative RNA processing regulators.
Mol Biol Cell. 17(12):5105-5114.
 Construct from CALCA [796] INT3 to 3'end fused with EX1 and half of INT1 from the major late transcription unit of adenovirus UV crosslink, immunoprecipitation, EMSA, Western blot with HeLa nuclear extracts.
TIAL1UUCAUUUUUGAUUCUUUGGUUAAAAAA-5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 19339348Choi EY, Pintel D. (2009)
Splicing of the large intron present in the non-structural gene of minute virus of mice (MVM) is governed by TIA-1/TIAR binding downstream of the non-consensus donor.
J Virol. 83(12):6306-6311
 Synthesized sequences. RNA chromatography affinity and immunoblotting in HeLa nuclear extract.
TIAL1AUUUA1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUC7Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUUA2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUUC2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUUG2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUUUA1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUUUUUC2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1AUUUUUUUUUUUA2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUA3Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUC10Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUG5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUA5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUC5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUG2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUUA1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUUC3Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUUG5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUUUC2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1CUUUUUUUUUUUC1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUC6Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUG5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUUC1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUUUC2Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUUUG1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUUUUUC1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1GUUUUUUUUG1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 8576255Dember LM, Kim ND, Liu KQ, Anderson P. (1996)
Individual RNA recognition motifs of TIA-1 and TIAR have different RNA binding specificities.
J Biol Chem. 271(5):2783-2788.
 Sequences of 68 nt random for SELEX. SELEX of 68nt random sequences with recombinant protein. Winners confirmed by UV cross-linking, competition assay.
TIAL1UUUUAAAUUUU-5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 12356730Iseni F, Garcin D, Nishio M, Kedersha N, Anderson P, Kolakofsky D. (2002)
Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis.
EMBO J. 21(19):5141-5150.
 Sendai virus (SeV) trailer (tr) RNA and mutants. UV cross-linking and immunoprecipitation with HeLa cytoplasmic extracts
TIAL1UUUUCAGUUUU-5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 12356730Iseni F, Garcin D, Nishio M, Kedersha N, Anderson P, Kolakofsky D. (2002)
Sendai virus trailer RNA binds TIAR, a cellular protein involved in virus-induced apoptosis.
EMBO J. 21(19):5141-5150.
 Sendai virus (SeV) trailer (tr) RNA and mutants. UV cross-linking and immunoprecipitation with HeLa cytoplasmic extracts
TIAL1AAAAUGCUUUUUUUUUUUUA-3Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 16091628Suswam EA, Li YY, Mahtani H, King PH. (2005)
Novel DNA-binding properties of the RNA-binding protein TIAR.
Nucleic Acids Res. 33(14):4507-4518.
 Sequences deriving from VEGFA [7422] UV cross-linking, competition assay with recombinant protein
TIAL1UAUUCUUUCUGAACAGUAUUUAAGGUUUCCAAUAAAAUGUACACCCCUCAG-5Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 16890199Izquierdo JM. (2006)
Control of the ATP synthase beta subunit expression by RNA-binding proteins TIA-1, TIAR, and HuR.
Biochem Biophys Res Commun. 348(2):703-711.
 Sequences deriving from ATP5B [506] UV cross-linking and immunoprecipitation with HeLa cellular extracts and recombinant protein.
TIAL1UUGCCACCUCCUGCUCCUGCCCAGACAG-10Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 17682065Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA. (2007)
Elucidation of a C-rich signature motif in target mRNAs of RNA-binding protein TIAR.
Mol Cell Biol. 27(19):6806-6817.
 Synthesized sequences Surface plasmon resonance
TIAL1GGGGGGUUUUUUUUUUUUUUUUUGGGGG-1Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 17682065Kim HS, Kuwano Y, Zhan M, Pullmann R Jr, Mazan-Mamczarz K, Li H, Kedersha N, Anderson P, Wilce MC, Gorospe M, Wilce JA. (2007)
Elucidation of a C-rich signature motif in target mRNAs of RNA-binding protein TIAR.
Mol Cell Biol. 27(19):6806-6817.
 Synthesized sequences Surface plasmon resonance
TIAL1GGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCCGGGGCC6Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
TIAL1AAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACCAAAACC4Gene Name and Synonymous: TIAL1, TIA1 cytotoxic granule-associated RNA binding protein-like 1, TCBP, TIAR, MGC33401. 23381195Mori K, Lammich S, Mackenzie IR, Forne I, Zilow S, Kretzschmar H, Edbauer D, Janssens J, Kleinberger G, Cruts M, Herms J, Neumann M, Van Broeckhoven C, Arzberger T, Haass C. (2013)
hnRNP A3 binds to GGGGCC repeats and is a constituent of p62-positive/TDP43-negative inclusions in the hippocampus of patients with C9orf72 mutations.
Acta Neuropathol. 125(3):413-423.
 Synthetic sequences. RNA pull down with HEK293 nuclear extract and LC–MS. Some protein confirmed by Western blot.
YB-1ACAAC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18503770Skoko N, Baralle M, Buratti E, Baralle FE. (2008)
The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors.
FEBS Lett. 582(15):2231-2236.
 Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37.In vivo splicing in HeLa, siRNA.UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts.
YB-1CACCAGUCACCGC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 11447123Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001)
The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.
EMBO J. 20(14):3821-3830.
 synthesized oligos UV crosslink and competition assay in HeLa nuclear extracts.
YB-1CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACA5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 12447348Hui J, Stangl K, Lane WS, Bindereif A. (2003)
HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats.
Nat Struct Biol. 10(1): 33-37.
In this context, CA repeats act as an unusual intronic splicing enhancer, whose activity depends on the CA repeat number. Construct of eNOS [NOS3, 4846] EX14 - INT14 - EX15 without CA-repeats or with 19, 32, 38 CA-repeats in INT14. Synthesized sequences.In vitro splicing in HeLa nuclear extract. In vivo splicing in HeLa, HEK293 and HUVEC cells. In vitro splicing in hnRNP L-depleted HeLa nuclear extract and complementation.UV crosslinking in HeLa nuclear extract, affinity purification and mass-spectrometry analysis.
YB-1CAACCACACCAC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 11447123Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001)
The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.
EMBO J. 20(14):3821-3830.
 synthesized oligos UV crosslink and competition assay in HeLa nuclear extracts.
YB-1ACCACACAAAACA5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 11447123Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001)
The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.
EMBO J. 20(14):3821-3830.
 synthesized oligos UV crosslink and competition assay in HeLa nuclear extracts.
YB-1CAACCACAA5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 11447123Stickeler E, Fraser SD, Honig A, Chen AL, Berget SM, Cooper TA. (2001)
The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.
EMBO J. 20(14):3821-3830.
 synthesized oligos UV crosslink and competition assay in HeLa nuclear extracts.
YB-1AGAAC2Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18503770Skoko N, Baralle M, Buratti E, Baralle FE. (2008)
The pathological splicing mutation c.6792C>G in NF1 exon 37 causes a change of tenancy between antagonistic splicing factors.
FEBS Lett. 582(15):2231-2236.
 Constructs spanning from NF1 [4763] EX34 to EX38 for in vivo splicing. Sequences of NF1 EX37.In vivo splicing in HeLa, siRNA.UV crosslink, EMSA, and pull-down assays with HeLa nuclear extracts.
YB-1CCUGCGG5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
YB-1GCCUGCG5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
YB-1CUGCGGU5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
YB-1GGUCUGC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
YB-1CCCUGCG5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 19561594Ray D, Kazan H, Chan ET, Pena Castillo L, Chaudhry S, Talukder S, Blencowe BJ, Morris Q, Hughes TR. (2009)
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Nat Biotechnol. 27(7):667-670.
 Synthesized sequences RNAcompete using recombinant protein
YB-1GGUCUCUCUGGUUAGACCUGAUCUGAGCCUGGGAGCUCUCUGGCUAACUAGGGAACC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 10573156Ansari SA, Safak M, Gallia GL, Sawaya BE, Amini S, Khalili K. (1999)
Interaction of YB-1 with human immunodeficiency virus type 1 Tat and TAR RNA modulates viral promoter activity.
J Gen Virol. 80 (Pt 10):2629-2638.
 Synthesized sequences and HIV-1 TAR EMSA with recombinant protein
YB-1GGCCUGAUCUGAGCCUGGGAGCUCUCUGGCC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 10573156Ansari SA, Safak M, Gallia GL, Sawaya BE, Amini S, Khalili K. (1999)
Interaction of YB-1 with human immunodeficiency virus type 1 Tat and TAR RNA modulates viral promoter activity.
J Gen Virol. 80 (Pt 10):2629-2638.
 Synthesized sequences and HIV-1 TAR EMSA with recombinant protein
YB-1CCCUCUGCCACCCAGGCAGGCCCUGCCUUCAGCCCUGGCCCAGAGCUGGAACACUCUCUGAGAUGCCCCUCUGCCUGGGCUUAUGCCCUCAGAUGGAGACAUUGGAUGUGGAGCUCCUGCUGGAUGCGUGCCCUGACCCCUGCACCAGCCCUUCCCUGCUUUGAG-5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 12600897Skalweit A, Doller A, Huth A, Kahne T, Persson PB, Thiele BJ. (2003)
Posttranscriptional control of renin synthesis: identification of proteins interacting with renin mRNA 3'-untranslated region.
Circ Res. 92(4):419-427.
 Sequences deriving from REN [5972] EMSA, UV cross-linking with Calu-6 cell extracts. RNA-affinity chromatography with MALDI-TOF-MS. Western blot.
YB-1UCUCGGGGGGGUUUUCAUCUAUGAGGGUGUUUCCUCUAAACCUACGAGGGAGGAACACCUGAUCUUACAGAAAAUACCACCUCGAGA5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 16508950Shen Q, Fan L, Newburger PE. (2006)
Nuclease sensitive element binding protein 1 associates with the selenocysteine insertion sequence and functions in mammalian selenoprotein translation.
J Cell Physiol. 207(3):775-783.
 Sequences deriving from GPX1 [2876] UV cross-linking, immunoprecipitation in HeLa
YB-1GCAGCCAGACAGCGAGGGCC-1Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18075498Fraser DJ, Phillips AO, Zhang X, van Roeyen CR, Muehlenberg P, En-Nia A, Mertens PR. (2008)
Y-box protein-1 controls transforming growth factor-beta1 translation in proximal tubular cells.
Kidney Int. 73(6):724-732.
 Sequences deriving from TGFB1 [7040] EMSA supershift, UV cross-linking, competition assay with HK-2 protein extracts.
YB-1GGGCACCCCCCCGGCUCUG-10Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18075498Fraser DJ, Phillips AO, Zhang X, van Roeyen CR, Muehlenberg P, En-Nia A, Mertens PR. (2008)
Y-box protein-1 controls transforming growth factor-beta1 translation in proximal tubular cells.
Kidney Int. 73(6):724-732.
 Sequences deriving from TGFB1 [7040] EMSA supershift, UV cross-linking, competition assay with HK-2 protein extracts.
YB-1CUGCACCACCACCUAUCUA2Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
YB-1GGCAGGAAGAAGAGGAGCA8Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
YB-1CCAGACACCGGAAACCCCUGCCACACCAC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 18945760Jia R, Liu X, Tao M, Kruhlak M, Guo M, Meyers C, Baker CC, Zheng ZM. (2009)
Control of the papillomavirus early-to-late switch by differentially expressed SRp20.
J Virol. 83(1):167-180.
 Sequences deriving from BPV-1 and HPV-16 mRNAs. EMSA, immunoprecipitation with HEK293 NE, U20S NE and U20S total cell extract.
YB-1CAUC10Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
YB-1CACC6Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
YB-1GAUC5Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
YB-1CAUCUG10Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
YB-1CAUCGC8Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
YB-1GAUCUG8Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
YB-1CAUCAUC10Gene Name and Synonymous: YBX1, Y box binding protein 1, YB1, BP-8, CSDB, DBPB, CSDA2, NSEP1, NSEP-1, MDR-NF1, MGC104858, MGC110976, MGC117250. 22730292Wei WJ, Mu SR, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J. (2012)
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.
Nucleic Acids Res. 40(17):8622-8636.
 Sequences of 20 nt random for SELEX. Constructs of wt and mutated CD44 [960] RSV - INTv4 - EXv5 - INTv5 - RSV In vivo splicing in HEK-293 cells.SELEX of 20nt random sequences with recombinant protein. Winners confirmed by EMSA with recombinant protein. In vivo splicing in HEK-293 cells with wt and mutated sequences, EMSA. UV crosslinking.
ZRANB2AGGUAA-10Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2CGGUAA-3Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2UGGUAA-2Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGUAG-2Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2CGGUCU-2Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGUCU-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2CGGUAU-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGUAC-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGUAU-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGUUA-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGUUU-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2CGGUAG-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2GAGGUU-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2GGGUAA-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2GGGUGA-1Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2AGGGAA-5Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
19304800Loughlin FE, Mansfield RE, Vaz PM, McGrath AP, Setiyaputra S, Gamsjaeger R, Chen ES, Morris BJ, Guss JM, Mackay JP. (2009)
The zinc fingers of the SR-like protein ZRANB2 are single-stranded RNA-binding domains that recognize 5' splice site-like sequences.
Proc Natl Acad Sci U S A. 106(14): 5581-5586.
Two close motifs make binding significantly stronger. Synthesized oligos. Sequences of 25nt random for SELEX. SELEX of 25nt random with recombinant protein. EMSA, Fluorescence Anisotropy, NMR Spectroscopy.
ZRANB2GGUGGU-10Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
22517726O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012)
Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA.
Angew Chem Int Ed Engl. 51(22):5371-5375.
 Synthesized sequences Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein
ZRANB2GGUAAAGGU-10Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
22517726O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012)
Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA.
Angew Chem Int Ed Engl. 51(22):5371-5375.
 Synthesized sequences Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein
ZRANB2GGUGGUGGU-10Gene Name and Synonymous: ZRANB2, zinc finger, RAN-binding domain containing 2, ZIS, ZIS1, ZIS2, ZNF265, FLJ41119, DKFZp686J183, DKFZp686N09117.
ZRANB2 (ZNF265) is an SR-like protein that induce exclusion of EX2 and EX3 from the Tra2beta1 pre-mRNA in HEK293 cell (PMID: 11448987).
22517726O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP. (2012)
Modular Assembly of RanBP2-Type Zinc Finger Domains to Target Single-Stranded RNA.
Angew Chem Int Ed Engl. 51(22):5371-5375.
 Synthesized sequences Fluorescence anisotropy titration, isothermal titration calorimetry and HSQC spectroscopy using recombinant protein